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Protein

Putative carboxymethylenebutenolidase

Gene

MexAM1_META1p1735

Organism
Methylobacterium extorquens (strain ATCC 14718 / DSM 1338 / AM1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

4-carboxymethylenebut-2-en-4-olide + H2O = 4-oxohex-2-enedioate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei170 – 1701By similarity
Active sitei227 – 2271By similarity
Active sitei259 – 2591By similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciMEXT272630:GBY6-1659-MONOMER.

Protein family/group databases

ESTHERimetex-dlhh. Dienelactone_hydrolase.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative carboxymethylenebutenolidase (EC:3.1.1.45)
Alternative name(s):
Dienelactone hydrolase
Short name:
DLH
Gene namesi
Ordered Locus Names:MexAM1_META1p1735
OrganismiMethylobacterium extorquens (strain ATCC 14718 / DSM 1338 / AM1)
Taxonomic identifieri272630 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
Proteomesi
  • UP000009081 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 4040Sequence analysisAdd
BLAST
Chaini41 – 291251Putative carboxymethylenebutenolidasePRO_0000007247Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP71505.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the dienelactone hydrolase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105CDZ. Bacteria.
COG0412. LUCA.
HOGENOMiHOG000157123.
KOiK01061.
OMAiCWGGRIS.
OrthoDBiEOG64FKC2.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR002925. Dienelactn_hydro.
[Graphical view]
PfamiPF01738. DLH. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P71505-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAFDADLRS LAAQTTLSRR TVIATSLATG FALAVQPVAA QTTIATDANG
60 70 80 90 100
LIAGEVKIPM QDGVIPAYRA MPAEGGPFPT ILVVQEIFGV HEHIKDVCRR
110 120 130 140 150
LAKLGYFALA PELYARQGDV STLTNIQQIV SEVVSKVPDA QVMSDLDAAV
160 170 180 190 200
AFAKGTGKAD TARLGITGFC WGGRITWLYA AHNPAVKAGV AWYGRLVGDS
210 220 230 240 250
SALMPKNPVD VAADLKAPVL GLYGGADQGI PVATIDRMKE ACRAAGKTCD
260 270 280 290
FVVYPEAGHA FHADYRPSYR AEPAQDGWKR LQDWFRQYGV A
Length:291
Mass (Da):31,053
Last modified:September 22, 2009 - v2
Checksum:i6A51DF49220B8A98
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti125 – 1251N → D in AAB58886 (PubMed:9168622).Curated
Sequence conflicti140 – 1401A → D in AAB58886 (PubMed:9168622).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U72662 Genomic DNA. Translation: AAB58886.1.
CP001510 Genomic DNA. Translation: ACS39579.1.
RefSeqiWP_003597626.1. NC_012808.1.

Genome annotation databases

EnsemblBacteriaiACS39579; ACS39579; MexAM1_META1p1735.
KEGGimea:Mex_1p1735.
PATRICi22509302. VBIMetExt101010_1726.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U72662 Genomic DNA. Translation: AAB58886.1.
CP001510 Genomic DNA. Translation: ACS39579.1.
RefSeqiWP_003597626.1. NC_012808.1.

3D structure databases

ProteinModelPortaliP71505.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

ESTHERimetex-dlhh. Dienelactone_hydrolase.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACS39579; ACS39579; MexAM1_META1p1735.
KEGGimea:Mex_1p1735.
PATRICi22509302. VBIMetExt101010_1726.

Phylogenomic databases

eggNOGiENOG4105CDZ. Bacteria.
COG0412. LUCA.
HOGENOMiHOG000157123.
KOiK01061.
OMAiCWGGRIS.
OrthoDBiEOG64FKC2.

Enzyme and pathway databases

BioCyciMEXT272630:GBY6-1659-MONOMER.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR002925. Dienelactn_hydro.
[Graphical view]
PfamiPF01738. DLH. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular and mutational analysis of a DNA region separating two methylotrophy gene clusters in Methylobacterium extorquens AM1."
    Chistoserdova L.V., Lidstrom M.E.
    Microbiology 143:1729-1736(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 14718 / DSM 1338 / AM1.

Entry informationi

Entry nameiDLHH_METEA
AccessioniPrimary (citable) accession number: P71505
Secondary accession number(s): C5B115
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: September 22, 2009
Last modified: November 11, 2015
This is version 79 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.