Reviewed, UniProtKB/Swiss-Prot P71505 (DLHH_METEA)

Names and origin

Protein names

Recommended name:
    Putative carboxymethylenebutenolidase
    EC=3.1.1.45
Alternative name(s):
    Dienelactone hydrolase
      Short name=DLH

Gene names

Ordered Locus Names:

MexAM1_META1p1735

Organism

Methylobacterium extorquens (strain ATCC 14718 / DSM 1338 / AM1) [Complete proteome] [HAMAP]

Taxonomic identifier

272630 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Methylobacteriaceae › Methylobacterium

Protein attributes

Sequence length	291 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	4-carboxymethylenebut-2-en-4-olide + H2O = 4-oxohex-2-enedioate.
Sequence similarities	Belongs to the dienelactone hydrolase family.

Ontologies

Keywords
Domain	Signal
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	carboxymethylenebutenolidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 40	40	Potential
	Chain	41 – 291	251	Putative carboxymethylenebutenolidase		PRO_0000007247
Sites
	Active site	170	1	By similarity
	Active site	227	1	By similarity
	Active site	259	1	By similarity
Experimental info
	Sequence conflict	125	1	N → D in AAB58886. Ref.1
	Sequence conflict	140	1	A → D in AAB58886. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P71505-1 [UniParc]. Last modified September 22, 2009. Version 2. Checksum: 6A51DF49220B8A98	FASTA	291	31,053
	10 20 30 40 50 60 MTAFDADLRS LAAQTTLSRR TVIATSLATG FALAVQPVAA QTTIATDANG LIAGEVKIPM 70 80 90 100 110 120 QDGVIPAYRA MPAEGGPFPT ILVVQEIFGV HEHIKDVCRR LAKLGYFALA PELYARQGDV 130 140 150 160 170 180 STLTNIQQIV SEVVSKVPDA QVMSDLDAAV AFAKGTGKAD TARLGITGFC WGGRITWLYA 190 200 210 220 230 240 AHNPAVKAGV AWYGRLVGDS SALMPKNPVD VAADLKAPVL GLYGGADQGI PVATIDRMKE 250 260 270 280 290 ACRAAGKTCD FVVYPEAGHA FHADYRPSYR AEPAQDGWKR LQDWFRQYGV A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular and mutational analysis of a DNA region separating two methylotrophy gene clusters in Methylobacterium extorquens AM1." Chistoserdova L.V., Lidstrom M.E. Microbiology 143:1729-1736(1997) [PubMed: 9168622] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Methylobacterium genome sequences: a reference blueprint to investigate microbial metabolism of C1 compounds from natural and industrial sources." Vuilleumier S., Chistoserdova L., Lee M.-C., Bringel F., Lajus A., Zhou Y., Gourion B., Barbe V., Chang J., Cruveiller S., Dossat C., Gillett W., Gruffaz C., Haugen E., Hourcade E., Levy R., Mangenot S., Muller E. , Nadalig T., Pagni M., Penny C., Peyraud R., Robinson D.G., Roche D., Rouy Z., Saenampechek C., Salvignol G., Vallenet D., Wu Z., Marx C.J., Vorholt J.A., Olson M.V., Kaul R., Weissenbach J., Medigue C., Lidstrom M.E. PLoS ONE 4:E5584-E5584(2009) [PubMed: 19440302] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	U72662 Genomic DNA. Translation: AAB58886.1. CP001510 Genomic DNA. Translation: ACS39579.1.
RefSeq	YP_002962856.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	7991698.
Organism-specific databases
CMR	Search...
Enzyme and pathway databases
BRENDA	3.1.1.45. 20440.
Family and domain databases
InterPro	IPR006311. TAT_signal. [Graphical view]
TIGRFAMs	TIGR01409. TAT_signal_seq. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DLHH_METEA

Accession

Primary (citable) accession number: P71505
Secondary accession number(s): C5B115

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	September 22, 2009
Last modified:	February 9, 2010
This is version 47 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)