P71454 (P71454_LEUME) Unreviewed, UniProtKB/TrEMBL
Last modified
December 14, 2011.
Version 62.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize orderNames and origin
| Protein names | Recommended name: D-alanine--D-alanine ligase 1 HAMAP MF_00047 EC=6.3.2.4 HAMAP MF_00047 | ||
| Gene names |
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| Organism | Leuconostoc mesenteroides EMBL AAB18338.1 | ||
| Taxonomic identifier | 1245 [NCBI] | ||
| Taxonomic lineage | Bacteria › Firmicutes › Lactobacillales › Leuconostoc |
Protein attributes
| Sequence length | 377 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Cell wall formation By similarity. HAMAP MF_00047 |
| Catalytic activity | ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP MF_00047 |
| Cofactor | Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP MF_00047 |
| Pathway | Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00047 |
| Subcellular location | Cytoplasm By similarity HAMAP MF_00047. |
| Sequence similarities | Belongs to the D-alanine--D-alanine ligase family. HAMAP MF_00047 Contains 1 ATP-grasp domain. HAMAP MF_00047 |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Regions | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Domain | 140 – 349 | 210 | ATP-grasp By similarity HAMAP MF_00047 | ||||||
| Nucleotide binding | 170 – 225 | 56 | ATP By similarity HAMAP MF_00047 | ||||||
| Nucleotide binding | 178 – 180 | 3 | ADP PDB 1EHI | ||||||
| Nucleotide binding | 216 – 219 | 4 | ADP PDB 1EHI | ||||||
Sites | |||||||||
| Metal binding | 303 | 1 | Magnesium or manganese 1 By similarity HAMAP MF_00047 | ||||||
| Metal binding | 316 | 1 | Magnesium or manganese 1 By similarity HAMAP MF_00047 | ||||||
| Metal binding | 316 | 1 | Magnesium or manganese 2 By similarity HAMAP MF_00047 | ||||||
| Metal binding | 318 | 1 | Magnesium or manganese 2 By similarity HAMAP MF_00047 | ||||||
| Binding site | 187 | 1 | ADP; via amide nitrogen PDB 1EHI | ||||||
| Binding site | 224 | 1 | ADP PDB 1EHI | ||||||
| Binding site | 316 | 1 | ADP PDB 1EHI | ||||||
Sequences
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References
| [1] | Park I.-S., Walsh C.T. Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 8293 EMBL AAB18338.1. |
| [2] | "Enzymes of vancomycin resistance: the structure of D-alanine-D-lactate ligase of naturally resistant Leuconostoc mesenteroides." Kuzin A.P., Sun T., Jorczak-Baillass J., Healy V.L., Walsh C.T., Knox J.R. Structure 8:463-470(2000) [PubMed: 10801495] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.38 ANGSTROMS) IN COMPLEX WITH ADP. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| EMBL GenBank DDBJ | U75444 Genomic DNA. Translation: AAB18338.1. | ||||||||||||
3D structure databases | |||||||||||||
| PDBe RCSB PDB PDBj |
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| ProteinModelPortal | P71454. | ||||||||||||
| SMR | P71454. Positions 3-362. | ||||||||||||
| ModBase | Search... | ||||||||||||
Protocols and materials databases | |||||||||||||
| StructuralBiologyKnowledgebase | Search... | ||||||||||||
Family and domain databases | |||||||||||||
| HAMAP | MF_00047. Dala_Dala_lig. [Tree] | ||||||||||||
| InterPro | IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR000291. D-Ala_lig_Van_CS. IPR005905. D_ala_D_ala. IPR011095. Dala_Dala_lig_C. IPR011127. Dala_Dala_lig_N. IPR013817. Pre-ATP_grasp. IPR016185. PreATP-grasp-like. [Graphical view] | ||||||||||||
| Gene3D | G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit. | ||||||||||||
| PANTHER | PTHR23132. PTHR23132. 1 hit. | ||||||||||||
| Pfam | PF07478. Dala_Dala_lig_C. 1 hit. PF01820. Dala_Dala_lig_N. 1 hit. [Graphical view] | ||||||||||||
| SUPFAM | SSF52440. PreATP-grasp-like. 1 hit. | ||||||||||||
| TIGRFAMs | TIGR01205. D_ala_D_alaTIGR. 1 hit. | ||||||||||||
| PROSITE | PS50975. ATP_GRASP. 1 hit. PS00843. DALA_DALA_LIGASE_1. 1 hit. PS00844. DALA_DALA_LIGASE_2. 1 hit. [Graphical view] | ||||||||||||
| ProtoNet | Search... | ||||||||||||
Entry information
| Entry name | P71454_LEUME | ||||||||
| Accession | Primary (citable) accession number: P71454 | ||||||||
| Entry history |
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| Entry status | Unreviewed (UniProtKB/TrEMBL) | ||||||||

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