P71447 (PGMB_LACLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-phosphoglucomutase
Short name=Beta-PGM
EC=5.4.2.6

Gene names

Name:	pgmB
Ordered Locus Names:	LL0429
ORF Names:	L0001

Organism

Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)

Taxonomic identifier

272623 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Lactococcus

Protein attributes

Sequence length	221 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Reversible transformation of glucose 6-phosphate and beta-glucose 1-phosphate.
Catalytic activity	Beta-D-glucose 1-phosphate = beta-D-glucose 6-phosphate.
Induction	By maltose, trehalose and sucrose and repressed by glucose and lactose.
Sequence similarities	Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family.
Biophysicochemical properties	Kinetic parameters: KM=4.6 µM for beta-glucose 1-phosphate (at pH 7 and 25 degrees Celsius)

Ontologies

Keywords
Molecular function	Isomerase
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Molecular function	beta-phosphoglucomutase activity Inferred from electronic annotation. Source: EC hydrolase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 221

221

Beta-phosphoglucomutase

PRO_0000108053

Experimental info

Mutagenesis

45

1

K → A: 20'000-fold decrease in Kcat/KM. Ref.3

Mutagenesis

46

1

G → A: 1'000'000-fold decrease in Kcat/KM. Ref.3

Mutagenesis

46

1

G → P: 100'000-fold decrease in Kcat/KM. Ref.3

Mutagenesis

46

1

G → V: 10'000-fold decrease in Kcat/KM. Ref.3

Mutagenesis

49

1

R → K: 1'000'000-fold decrease in Kcat/KM. Ref.3

Mutagenesis

52

1

S → A: Wild-type activity. Ref.3

Sequence conflict

125

1

K → R in CAA94734. Ref.1

Sequence conflict

206

1

Y → H in CAA94734. Ref.1

Secondary structure

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221

Helix Strand Turn

Details...

Beta strand

4 – 7

4

Turn

10 – 12

3

Beta strand

13 – 15

3

Helix

16 – 30

15

Helix

38 – 41

4

Turn

42 – 46

5

Helix

49 – 57

9

Beta strand

60 – 62

3

Helix

66 – 83

18

Turn

84 – 86

3

Helix

89 – 91

3

Helix

96 – 105

10

Beta strand

109 – 112

4

Helix

119 – 125

7

Helix

129 – 131

3

Beta strand

133 – 135

3

Turn

138 – 140

3

Beta strand

141 – 143

3

Helix

149 – 157

9

Helix

162 – 164

3

Beta strand

165 – 171

7

Helix

172 – 181

10

Beta strand

184 – 189

6

Helix

191 – 194

4

Beta strand

196 – 203

8

Helix

204 – 206

3

Helix

209 – 216

8

Sequences

	Sequence		Length	Mass (Da)
	P71447 [UniParc]. Last modified April 27, 2001. Version 2. Checksum: 53AC0BF0FA249EFC	FASTA	221	24,209
	10 20 30 40 50 60 MFKAVLFDLD GVITDTAEYH FRAWKALAEE IGINGVDRQF NEQLKGVSRE DSLQKILDLA 70 80 90 100 110 120 DKKVSAEEFK ELAKRKNDNY VKMIQDVSPA DVYPGILQLL KDLRSNKIKI ALASASKNGP 130 140 150 160 170 180 FLLEKMNLTG YFDAIADPAE VAASKPAPDI FIAAAHAVGV APSESIGLED SQAGIQAIKD 190 200 210 220 SGALPIGVGR PEDLGDDIVI VPDTSYYTLE FLKEVWLQKQ K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Product formation and phosphoglucomutase activities in Lactococcus lactis: cloning and characterization of a novel phosphoglucomutase gene." Qian N., Stanley G.A., Bunte A., Raadstroem P. Microbiology 143:855-865(1997) [PubMed: 9084169] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-15. Strain: ATCC 19435 / DSM 20481 / NCDO 604 / NCIB 6681 / NCTC 6681.
[2]	"The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403." Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A. Genome Res. 11:731-753(2001) [PubMed: 11337471] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IL1403.
[3]	"Analysis of the substrate specificity loop of the HAD superfamily cap domain." Lahiri S.D., Zhang G., Dai J., Dunaway-Mariano D., Allen K.N. Biochemistry 43:2812-2820(2004) [PubMed: 15005616] [Abstract] Cited for: BIOPHYSICOCHEMICAL CHARACTERIZATION, MUTAGENESIS OF LYS-45; GLY-46; ARG-49 AND SER-52.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Z70730 Genomic DNA. Translation: CAA94734.1.
AE005176 Genomic DNA. Translation: AAK04527.1.

PIR

E86678.

RefSeq

NP_266585.1. NC_002662.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1LVH	X-ray	2.30	A/B	1-221	[»]
1O03	X-ray	1.40	A	1-221	[»]
1O08	X-ray	1.20	A	1-221	[»]
1Z4N	X-ray	1.97	A/B	1-221	[»]
1Z4O	X-ray	1.90	A/B	1-221	[»]
1ZOL	X-ray	1.90	A	1-221	[»]
2WF5	X-ray	1.30	A	1-221	[»]
2WF6	X-ray	1.40	A	1-221	[»]
2WF7	X-ray	1.05	A	1-221	[»]
2WF8	X-ray	1.20	A	1-221	[»]
2WF9	X-ray	1.40	A	1-221	[»]
2WFA	X-ray	1.65	A	1-221	[»]
2WHE	X-ray	1.55	A	1-221	[»]
3FM9	X-ray	2.70	A	1-221	[»]

ProteinModelPortal

P71447.

SMR

P71447. Positions 1-221.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

1114041.

GenomeReviews

Gene locus LL0429 in contig AE005176_GR.

KEGG

lla:L0001.

NMPDR

fig|272623.1.peg.438.

PATRIC

22293074. VBILacLac136773_0467.

Phylogenomic databases

HOGENOM

HBG742904.

OMA

YIVDANE.

ProtClustDB

CLSK876745.

Enzyme and pathway databases

BioCyc

LLAC272623:L0001-MONOMER.
MetaCyc:MONOMER-5821.

BRENDA

5.4.2.6. 2903.

Family and domain databases

InterPro

IPR010976. B-phosphoglucomutase_hydrolase.
IPR010972. Beta-phosphoglucomutase.
IPR005834. Dehalogen-like_hydro.
IPR023214. HAD-like_dom.
IPR006402. HAD-SF_hydro_IA_v3.
IPR005833. Haloacid_DH/epoxide_hydro.
[Graphical view]

Gene3D

G3DSA:3.40.50.1000. HAD-like_dom. 2 hits.

KO

K01838.

Pfam

PF00702. Hydrolase. 1 hit.
[Graphical view]

PRINTS

PR00413. HADHALOGNASE.

SUPFAM

SSF56784. HAD-like_dom. 1 hit.

TIGRFAMs

TIGR01990. BPGM. 1 hit.
TIGR01509. HAD-SF-IA-v3. 1 hit.
TIGR02009. PGMB-YQAB-SF. 1 hit.

ProtoNet

Search...

Entry information

Entry name

PGMB_LACLA

Accession

Primary (citable) accession number: P71447

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	April 27, 2001
Last modified:	January 25, 2012
This is version 91 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program