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Protein

ATP-dependent zinc metalloprotease FtsH

Gene

ftsH

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins. Plays a role in the quality control of integral membrane proteins.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei173ATP; via amide nitrogen and carbonyl oxygen1
Binding sitei354ATP1
Metal bindingi434Zinc; catalyticUniRule annotation1
Active sitei435UniRule annotation1
Metal bindingi438Zinc; catalyticUniRule annotation1
Metal bindingi511Zinc; catalyticUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi213 – 217ATP5

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciHPY:HP1069-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent zinc metalloprotease FtsHUniRule annotation (EC:3.4.24.-UniRule annotation)
Gene namesi
Name:ftsHUniRule annotation
Ordered Locus Names:HP_1069
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
Proteomesi
  • UP000000429 Componenti: Chromosome

Subcellular locationi

  • Cell inner membrane 1 Publication; Multi-pass membrane protein 1 Publication; Cytoplasmic side 1 Publication

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 9CytoplasmicUniRule annotation9
Transmembranei10 – 30HelicalUniRule annotationAdd BLAST21
Topological domaini31 – 116PeriplasmicUniRule annotationAdd BLAST86
Transmembranei117 – 137HelicalUniRule annotationAdd BLAST21
Topological domaini138 – 632CytoplasmicCuratedAdd BLAST495

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

A homozygous disruption strain was not identified, suggesting this gene may be essential.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000846361 – 632ATP-dependent zinc metalloprotease FtsHAdd BLAST632

Proteomic databases

PaxDbiP71408.

Interactioni

Subunit structurei

Homohexamer.UniRule annotation

Protein-protein interaction databases

DIPiDIP-3549N.
IntActiP71408. 4 interactors.
MINTiMINT-188002.
STRINGi85962.HP1069.

Structurei

Secondary structure

1632
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi169 – 171Combined sources3
Beta strandi172 – 174Combined sources3
Turni176 – 178Combined sources3
Helixi179 – 190Combined sources12
Helixi192 – 198Combined sources7
Beta strandi206 – 209Combined sources4
Beta strandi211 – 215Combined sources5
Helixi216 – 224Combined sources9
Turni225 – 228Combined sources4
Beta strandi231 – 234Combined sources4
Turni238 – 241Combined sources4
Beta strandi244 – 246Combined sources3
Beta strandi248 – 252Combined sources5
Helixi255 – 260Combined sources6
Beta strandi265 – 269Combined sources5
Helixi271 – 273Combined sources3
Turni291 – 293Combined sources3
Turni294 – 302Combined sources9
Beta strandi307 – 309Combined sources3
Beta strandi312 – 315Combined sources4
Helixi325 – 328Combined sources4
Beta strandi329 – 333Combined sources5
Beta strandi335 – 338Combined sources4
Turni344 – 346Combined sources3
Helixi347 – 354Combined sources8
Beta strandi356 – 358Combined sources3
Turni366 – 370Combined sources5
Beta strandi371 – 373Combined sources3
Helixi378 – 390Combined sources13
Beta strandi393 – 395Combined sources3
Helixi402 – 406Combined sources5
Beta strandi408 – 411Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2R62X-ray3.30A/B160-419[»]
2R65X-ray3.30A/B/C/D/E160-419[»]
ProteinModelPortaliP71408.
SMRiP71408.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP71408.

Family & Domainsi

Sequence similaritiesi

In the central section; belongs to the AAA ATPase family.UniRule annotation
In the C-terminal section; belongs to the peptidase M41 family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105C3H. Bacteria.
COG0465. LUCA.
KOiK03798.
OMAiIAMITSF.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_01458. FtsH. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR005936. FtsH.
IPR027417. P-loop_NTPase.
IPR000642. Peptidase_M41.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF01434. Peptidase_M41. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01241. FtsH_fam. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P71408-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKPTNEPKKP FFQSPIILAV LGGILLIFFL RSFNSDGSFS DNFLASSTKN
60 70 80 90 100
VSYHEIKQLI SNNEVENVSI GQTLIKASHK EGNNRVIYIA KRVPDLTLVP
110 120 130 140 150
LLDEKKINYS GFSESNFFTD MLGWLMPILV ILGLWMFMAN RMQKNMGGGI
160 170 180 190 200
FGMGSAKKLI NAEKPNVRFN DMAGNEEAKE EVVEIVDFLK YPERYANLGA
210 220 230 240 250
KIPKGVLLVG PPGTGKTLLA KAVAGEAHVP FFSMGGSSFI EMFVGLGASR
260 270 280 290 300
VRDLFETAKK QAPSIIFIDE IDAIGKSRAA GGVVSGNDER EQTLNQLLAE
310 320 330 340 350
MDGFGSENAP VIVLAATNRP EILDPALMRP GRFDRQVLVD KPDFNGRVEI
360 370 380 390 400
LKVHIKGVKL ANDVNLQEVA KLTAGLAGAD LANIINEAAL LAGRNNQKEV
410 420 430 440 450
RQQHLKEAVE RGIAGLEKKS RRISPKEKKI VAYHESGHAV ISEMTKGSAR
460 470 480 490 500
VNKVSIIPRG MAALGYTLNT PEENKYLMQK HELIAEIDVL LGGRAAEDVF
510 520 530 540 550
LEEISTGASN DLERATDIIK GMVSYYGMSS VSGLMVLEKQ RNAFLGGGYG
560 570 580 590 600
SSREFSEKTA EEMDLFIKNL LEERYKHVKQ TLSDYREAIE IMVKELFDKE
610 620 630
VITGERVREI ISEYEVANNL ESRLIPLEEQ AS
Length:632
Mass (Da):69,745
Last modified:November 1, 1997 - v2
Checksum:i1D0674163FFC206D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti275G → P in AAC44563 (PubMed:8892813).Curated1
Sequence conflicti283 – 284VV → MI in AAC44563 (PubMed:8892813).Curated2
Sequence conflicti297L → F in AAB66377 (PubMed:9244252).Curated1
Sequence conflicti354H → D in AAC44563 (PubMed:8892813).Curated1
Sequence conflicti366 – 367LQ → FE in AAC44563 (PubMed:8892813).Curated2
Sequence conflicti401 – 402RQ → KL in AAB66377 (PubMed:9244252).Curated2
Sequence conflicti401R → K in AAC44563 (PubMed:8892813).Curated1
Sequence conflicti430I → M in AAB66377 (PubMed:9244252).Curated1
Sequence conflicti449A → G in AAB66377 (PubMed:9244252).Curated1
Sequence conflicti455S → Y in AAB66377 (PubMed:9244252).Curated1
Sequence conflicti499V → F in AAB66377 (PubMed:9244252).Curated1
Sequence conflicti576K → E in AAC44563 (PubMed:8892813).Curated1
Sequence conflicti576K → E in AAB05472 (PubMed:8550601).Curated1
Sequence conflicti616V → A in AAC44563 (PubMed:8892813).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U59452 Genomic DNA. Translation: AAC44563.1.
U97567 Genomic DNA. Translation: AAB66377.1.
AE000511 Genomic DNA. Translation: AAD08115.1.
U59625 Genomic DNA. Translation: AAB05472.1.
PIRiE64653.
RefSeqiNP_207860.1. NC_000915.1.
WP_000805281.1. NC_018939.1.

Genome annotation databases

EnsemblBacteriaiAAD08115; AAD08115; HP_1069.
GeneIDi899605.
KEGGiheo:C694_05525.
hpy:HP1069.
PATRICi20593477. VBIHelPyl33062_1118.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U59452 Genomic DNA. Translation: AAC44563.1.
U97567 Genomic DNA. Translation: AAB66377.1.
AE000511 Genomic DNA. Translation: AAD08115.1.
U59625 Genomic DNA. Translation: AAB05472.1.
PIRiE64653.
RefSeqiNP_207860.1. NC_000915.1.
WP_000805281.1. NC_018939.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2R62X-ray3.30A/B160-419[»]
2R65X-ray3.30A/B/C/D/E160-419[»]
ProteinModelPortaliP71408.
SMRiP71408.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-3549N.
IntActiP71408. 4 interactors.
MINTiMINT-188002.
STRINGi85962.HP1069.

Proteomic databases

PaxDbiP71408.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD08115; AAD08115; HP_1069.
GeneIDi899605.
KEGGiheo:C694_05525.
hpy:HP1069.
PATRICi20593477. VBIHelPyl33062_1118.

Phylogenomic databases

eggNOGiENOG4105C3H. Bacteria.
COG0465. LUCA.
KOiK03798.
OMAiIAMITSF.

Enzyme and pathway databases

BioCyciHPY:HP1069-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP71408.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_01458. FtsH. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR005936. FtsH.
IPR027417. P-loop_NTPase.
IPR000642. Peptidase_M41.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF01434. Peptidase_M41. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01241. FtsH_fam. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFTSH_HELPY
AccessioniPrimary (citable) accession number: P71408
Secondary accession number(s): O07679, Q48268
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.