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Protein

ATP-dependent zinc metalloprotease FtsH

Gene

ftsH

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins. Plays a role in the quality control of integral membrane proteins.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei173 – 1731ATP; via amide nitrogen and carbonyl oxygen
Binding sitei354 – 3541ATP
Metal bindingi434 – 4341Zinc; catalyticUniRule annotation
Active sitei435 – 4351UniRule annotation
Metal bindingi438 – 4381Zinc; catalyticUniRule annotation
Metal bindingi511 – 5111Zinc; catalyticUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi213 – 2175ATP

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciHPY:HP1069-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent zinc metalloprotease FtsHUniRule annotation (EC:3.4.24.-UniRule annotation)
Gene namesi
Name:ftsHUniRule annotation
Ordered Locus Names:HP_1069
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
Proteomesi
  • UP000000429 Componenti: Chromosome

Subcellular locationi

  • Cell inner membrane 1 Publication; Multi-pass membrane protein 1 Publication; Cytoplasmic side 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 99CytoplasmicUniRule annotation
Transmembranei10 – 3021HelicalUniRule annotationAdd
BLAST
Topological domaini31 – 11686PeriplasmicUniRule annotationAdd
BLAST
Transmembranei117 – 13721HelicalUniRule annotationAdd
BLAST
Topological domaini138 – 632495CytoplasmicCuratedAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

A homozygous disruption strain was not identified, suggesting this gene may be essential.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 632632ATP-dependent zinc metalloprotease FtsHPRO_0000084636Add
BLAST

Proteomic databases

PaxDbiP71408.
PRIDEiP71408.

Interactioni

Subunit structurei

Homohexamer.UniRule annotation

Protein-protein interaction databases

DIPiDIP-3549N.
IntActiP71408. 4 interactions.
MINTiMINT-188002.
STRINGi85962.HP1069.

Structurei

Secondary structure

1
632
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi169 – 1713Combined sources
Beta strandi172 – 1743Combined sources
Turni176 – 1783Combined sources
Helixi179 – 19012Combined sources
Helixi192 – 1987Combined sources
Beta strandi206 – 2094Combined sources
Beta strandi211 – 2155Combined sources
Helixi216 – 2249Combined sources
Turni225 – 2284Combined sources
Beta strandi231 – 2344Combined sources
Turni238 – 2414Combined sources
Beta strandi244 – 2463Combined sources
Beta strandi248 – 2525Combined sources
Helixi255 – 2606Combined sources
Beta strandi265 – 2695Combined sources
Helixi271 – 2733Combined sources
Turni291 – 2933Combined sources
Turni294 – 3029Combined sources
Beta strandi307 – 3093Combined sources
Beta strandi312 – 3154Combined sources
Helixi325 – 3284Combined sources
Beta strandi329 – 3335Combined sources
Beta strandi335 – 3384Combined sources
Turni344 – 3463Combined sources
Helixi347 – 3548Combined sources
Beta strandi356 – 3583Combined sources
Turni366 – 3705Combined sources
Beta strandi371 – 3733Combined sources
Helixi378 – 39013Combined sources
Beta strandi393 – 3953Combined sources
Helixi402 – 4065Combined sources
Beta strandi408 – 4114Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2R62X-ray3.30A/B160-419[»]
2R65X-ray3.30A/B/C/D/E160-419[»]
ProteinModelPortaliP71408.
SMRiP71408. Positions 163-408.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP71408.

Family & Domainsi

Sequence similaritiesi

In the central section; belongs to the AAA ATPase family.UniRule annotation
In the C-terminal section; belongs to the peptidase M41 family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105C3H. Bacteria.
COG0465. LUCA.
KOiK03798.
OMAiFFQSPIV.
OrthoDBiEOG6PKFBJ.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_01458. FtsH.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR005936. FtsH.
IPR027417. P-loop_NTPase.
IPR000642. Peptidase_M41.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF01434. Peptidase_M41. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01241. FtsH_fam. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P71408-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKPTNEPKKP FFQSPIILAV LGGILLIFFL RSFNSDGSFS DNFLASSTKN
60 70 80 90 100
VSYHEIKQLI SNNEVENVSI GQTLIKASHK EGNNRVIYIA KRVPDLTLVP
110 120 130 140 150
LLDEKKINYS GFSESNFFTD MLGWLMPILV ILGLWMFMAN RMQKNMGGGI
160 170 180 190 200
FGMGSAKKLI NAEKPNVRFN DMAGNEEAKE EVVEIVDFLK YPERYANLGA
210 220 230 240 250
KIPKGVLLVG PPGTGKTLLA KAVAGEAHVP FFSMGGSSFI EMFVGLGASR
260 270 280 290 300
VRDLFETAKK QAPSIIFIDE IDAIGKSRAA GGVVSGNDER EQTLNQLLAE
310 320 330 340 350
MDGFGSENAP VIVLAATNRP EILDPALMRP GRFDRQVLVD KPDFNGRVEI
360 370 380 390 400
LKVHIKGVKL ANDVNLQEVA KLTAGLAGAD LANIINEAAL LAGRNNQKEV
410 420 430 440 450
RQQHLKEAVE RGIAGLEKKS RRISPKEKKI VAYHESGHAV ISEMTKGSAR
460 470 480 490 500
VNKVSIIPRG MAALGYTLNT PEENKYLMQK HELIAEIDVL LGGRAAEDVF
510 520 530 540 550
LEEISTGASN DLERATDIIK GMVSYYGMSS VSGLMVLEKQ RNAFLGGGYG
560 570 580 590 600
SSREFSEKTA EEMDLFIKNL LEERYKHVKQ TLSDYREAIE IMVKELFDKE
610 620 630
VITGERVREI ISEYEVANNL ESRLIPLEEQ AS
Length:632
Mass (Da):69,745
Last modified:November 1, 1997 - v2
Checksum:i1D0674163FFC206D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti275 – 2751G → P in AAC44563 (PubMed:8892813).Curated
Sequence conflicti283 – 2842VV → MI in AAC44563 (PubMed:8892813).Curated
Sequence conflicti297 – 2971L → F in AAB66377 (PubMed:9244252).Curated
Sequence conflicti354 – 3541H → D in AAC44563 (PubMed:8892813).Curated
Sequence conflicti366 – 3672LQ → FE in AAC44563 (PubMed:8892813).Curated
Sequence conflicti401 – 4022RQ → KL in AAB66377 (PubMed:9244252).Curated
Sequence conflicti401 – 4011R → K in AAC44563 (PubMed:8892813).Curated
Sequence conflicti430 – 4301I → M in AAB66377 (PubMed:9244252).Curated
Sequence conflicti449 – 4491A → G in AAB66377 (PubMed:9244252).Curated
Sequence conflicti455 – 4551S → Y in AAB66377 (PubMed:9244252).Curated
Sequence conflicti499 – 4991V → F in AAB66377 (PubMed:9244252).Curated
Sequence conflicti576 – 5761K → E in AAC44563 (PubMed:8892813).Curated
Sequence conflicti576 – 5761K → E in AAB05472 (PubMed:8550601).Curated
Sequence conflicti616 – 6161V → A in AAC44563 (PubMed:8892813).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U59452 Genomic DNA. Translation: AAC44563.1.
U97567 Genomic DNA. Translation: AAB66377.1.
AE000511 Genomic DNA. Translation: AAD08115.1.
U59625 Genomic DNA. Translation: AAB05472.1.
PIRiE64653.
RefSeqiNP_207860.1. NC_000915.1.
WP_000805281.1. NC_018939.1.

Genome annotation databases

EnsemblBacteriaiAAD08115; AAD08115; HP_1069.
GeneIDi899605.
KEGGiheo:C694_05525.
hpy:HP1069.
PATRICi20593477. VBIHelPyl33062_1118.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U59452 Genomic DNA. Translation: AAC44563.1.
U97567 Genomic DNA. Translation: AAB66377.1.
AE000511 Genomic DNA. Translation: AAD08115.1.
U59625 Genomic DNA. Translation: AAB05472.1.
PIRiE64653.
RefSeqiNP_207860.1. NC_000915.1.
WP_000805281.1. NC_018939.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2R62X-ray3.30A/B160-419[»]
2R65X-ray3.30A/B/C/D/E160-419[»]
ProteinModelPortaliP71408.
SMRiP71408. Positions 163-408.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-3549N.
IntActiP71408. 4 interactions.
MINTiMINT-188002.
STRINGi85962.HP1069.

Proteomic databases

PaxDbiP71408.
PRIDEiP71408.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD08115; AAD08115; HP_1069.
GeneIDi899605.
KEGGiheo:C694_05525.
hpy:HP1069.
PATRICi20593477. VBIHelPyl33062_1118.

Phylogenomic databases

eggNOGiENOG4105C3H. Bacteria.
COG0465. LUCA.
KOiK03798.
OMAiFFQSPIV.
OrthoDBiEOG6PKFBJ.

Enzyme and pathway databases

BioCyciHPY:HP1069-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP71408.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_01458. FtsH.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR005936. FtsH.
IPR027417. P-loop_NTPase.
IPR000642. Peptidase_M41.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF01434. Peptidase_M41. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01241. FtsH_fam. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequencing, expression, and genetic characterization of the Helicobacter pylori ftsH gene encoding a protein homologous to members of a novel putative ATPase family."
    Ge Z., Taylor D.E.
    J. Bacteriol. 178:6151-6157(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
    Strain: ATCC 43629 / JCM 7656 / NCTC 11639 / UA802.
  2. "Identification and characterization of an operon of Helicobacter pylori that is involved in motility and stress adaptation."
    Beier D., Spohn G., Rappuoli R., Scarlato V.
    J. Bacteriol. 179:4676-4683(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: DSM 4867 / CCUG 17874 / NCTC 11638.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700392 / 26695.
  4. "Cloning and membrane topology of a P type ATPase from Helicobacter pylori."
    Melchers K., Weitzenegger T., Buhmann A., Steinhilber W., Sachs G., Schaefer K.P.
    J. Biol. Chem. 271:446-457(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 324-632.
    Strain: 69A.
  5. "Structural studies on Helicobacter pylori ATP-dependent protease, FtsH."
    Kim S.H., Kang G.B., Song H.E., Park S.J., Bea M.H., Eom S.H.
    J. Synchrotron Radiat. 15:208-210(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.3 ANGSTROMS) OF 160-419 NUCLEOTIDE-FREE AND COMPLEXED WITH ADP.

Entry informationi

Entry nameiFTSH_HELPY
AccessioniPrimary (citable) accession number: P71408
Secondary accession number(s): O07679, Q48268
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 11, 2015
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.