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Protein

Anthranilate synthase component 2

Gene

trpG

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Part of a heterotetrameric complex that catalyzes the two-step biosynthesis of anthranilate, an intermediate in the biosynthesis of L-tryptophan. In the first step, the glutamine-binding beta subunit (TrpG) of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by the large alpha subunit of AS (TrpE) to produce anthranilate. In the absence of TrpG, TrpE can synthesize anthranilate directly from chorismate and high concentrations of ammonia (By similarity).By similarity

Catalytic activityi

Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.

Pathwayi: L-tryptophan biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-tryptophan from chorismate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Putative anthranilate synthase component II (HI_1171), Anthranilate synthase component 2 (trpG), Anthranilate synthase component 1 (trpE)
  2. Anthranilate phosphoribosyltransferase (trpD)
  3. Tryptophan biosynthesis protein TrpCF (trpC)
  4. Tryptophan biosynthesis protein TrpCF (trpC)
  5. Tryptophan synthase alpha chain (trpA), Tryptophan synthase beta chain (trpB)
This subpathway is part of the pathway L-tryptophan biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tryptophan from chorismate, the pathway L-tryptophan biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei84Nucleophile; for GATase activityPROSITE-ProRule annotation1
Binding sitei88GlutamineBy similarity1
Active sitei170For GATase activityPROSITE-ProRule annotation1
Active sitei172For GATase activityPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processAmino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Enzyme and pathway databases

BioCyciHINF71421:G1GJ1-1414-MONOMER
UniPathwayiUPA00035; UER00040

Names & Taxonomyi

Protein namesi
Recommended name:
Anthranilate synthase component 2 (EC:4.1.3.27)
Short name:
AS
Short name:
ASII
Alternative name(s):
Anthranilate synthase, GATase component
Anthranilate synthase, glutamine amidotransferase component
Gene namesi
Name:trpG
Ordered Locus Names:HI_1388
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
Proteomesi
  • UP000000579 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000568781 – 193Anthranilate synthase component 2Add BLAST193

Interactioni

Subunit structurei

Heterotetramer consisting of two non-identical subunits: a beta subunit (TrpG) and a large alpha subunit (TrpE).By similarity

Protein-protein interaction databases

STRINGi71421.HI1388

Structurei

3D structure databases

ProteinModelPortaliP71381
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 193Glutamine amidotransferase type-1PROSITE-ProRule annotationAdd BLAST191

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni57 – 59Glutamine bindingBy similarity3
Regioni134 – 135Glutamine bindingBy similarity2

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiENOG4105DDQ Bacteria
COG0512 LUCA
KOiK01658
OMAiHQVVIYR
PhylomeDBiP71381

Family and domain databases

Gene3Di3.40.50.880, 1 hit
InterProiView protein in InterPro
IPR029062 Class_I_gatase-like
IPR017926 GATASE
IPR006221 TrpG/PapA_dom
PfamiView protein in Pfam
PF00117 GATase, 1 hit
SUPFAMiSSF52317 SSF52317, 1 hit
TIGRFAMsiTIGR00566 trpG_papA, 1 hit
PROSITEiView protein in PROSITE
PS51273 GATASE_TYPE_1, 1 hit

Sequencei

Sequence statusi: Complete.

P71381-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANILFLDNF DSFTYNLVDQ FRVLGHNVTI YRNDCDLEKL VETALNTPDT
60 70 80 90 100
ILALSPGPGT PSEAGILLPL IERLKNQVPI IGVCLGHQAL IQAFGGKVVH
110 120 130 140 150
AGEVLHGKVS RISHDNEAMF KDLANPMPVA RYHSLMGQDL PKEFIVNAEY
160 170 180 190
NGIIMAIRHR DLPICAFQFH PESILTVQGS QLLQQSIEWL LNR
Length:193
Mass (Da):21,509
Last modified:February 1, 1997 - v1
Checksum:i53FF77844D568B51
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA Translation: AAC23034.1
PIRiD64121
RefSeqiNP_439540.1, NC_000907.1
WP_005693974.1, NC_000907.1

Genome annotation databases

EnsemblBacteriaiAAC23034; AAC23034; HI_1388
GeneIDi950293
KEGGihin:HI1388
PATRICifig|71421.8.peg.1445

Similar proteinsi

Entry informationi

Entry nameiTRPG_HAEIN
AccessioniPrimary (citable) accession number: P71381
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: February 1, 1997
Last modified: March 28, 2018
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health