P71342 (NQRE_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Na(+)-translocating NADH-quinone reductase subunit E
Short name=Na(+)-NQR subunit E
Short name=Na(+)-translocating NQR subunit E
EC=1.6.5.-

Alternative name(s):
NQR complex subunit E
NQR-1 subunit E

Gene names

Name:	nqrE
Ordered Locus Names:	HI_0170

Organism

Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) [Reference proteome] [HAMAP]

Taxonomic identifier

71421 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus › Haemophilus influenzae

Protein attributes

Sequence length	198 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	NQR complex catalyzes the reduction of ubiquinone-1 to ubiquinol by two successive reactions, coupled with the transport of Na+ ions from the cytoplasm to the periplasm. NqrA to NqrE are probably involved in the second step, the conversion of ubisemiquinone to ubiquinol. HAMAP-Rule MF_00429
Catalytic activity	NADH + ubiquinone + Na+(In) = NAD+ + ubiquinol + Na+(Out). HAMAP-Rule MF_00429
Subunit structure	Composed of six subunits; NqrA, NqrB, NqrC, NqrD, NqrE and NqrF By similarity.
Subcellular location	Cell inner membrane; Multi-pass membrane protein Potential HAMAP-Rule MF_00429.
Sequence similarities	Belongs to the NqrDE/RnfAE family.

Ontologies

Keywords
Biological process	Ion transport Sodium transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	NAD Sodium Ubiquinone
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	respiratory electron transport chain Inferred from electronic annotation. Source: InterPro sodium ion transport Inferred from electronic annotation. Source: HAMAP
Cellular_component	Gram-negative-bacterium-type cell wall Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 198	198	Na(+)-translocating NADH-quinone reductase subunit E HAMAP-Rule MF_00429		PRO_0000214252
Regions
	Transmembrane	11 – 31	21	Helical; Potential
	Transmembrane	35 – 55	21	Helical; Potential
	Transmembrane	77 – 97	21	Helical; Potential
	Transmembrane	110 – 130	21	Helical; Potential
	Transmembrane	140 – 160	21	Helical; Potential
	Transmembrane	176 – 196	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	P71342 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 29C6544D4BF71BDE	FASTA	198	21,306
	10 20 30 40 50 60 MEHYISLFVK AVFIENMALS FFLGMCTFLA VSKKVSPAFG LGIAVTFVLG IAVPVNQLIY 70 80 90 100 110 120 ANVLKENALI EGVDLSFLNF ITFIGVIAGL VQILEMVLDK FMPSLYNALG IFLPLIAVNC 130 140 150 160 170 180 AIFGGVSFMV QRDYNFPESI VYGFGSGLGW MLAIVALAGL TEKMKYADIP AGLKGLGITF 190 ISVGLMALGF MSFSGIQL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
[2]	"Existence of Na+-translocating NADH-quinone reductase in Haemophilus influenzae." Hayashi M., Nakayama Y., Unemoto T. FEBS Lett. 381:174-176(1996) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION AS NQR SYSTEM. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L42023 Genomic DNA. Translation: AAC21840.1.
RefSeq	NP_438338.1. NC_000907.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	71421.HI0170.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC21840; AAC21840; HI_0170.
GeneID	951077.
KEGG	hin:HI0170.
PATRIC	20188835. VBIHaeInf48452_0174.
Phylogenomic databases
eggNOG	COG2209.
KO	K00350.
OMA	KYFPALY.
ProtClustDB	PRK02830.
Family and domain databases
HAMAP	MF_00429. NqrE.
InterPro	IPR003667. Elect_transpt_cplx_Rnf/Nqr. IPR010967. NADH_UbQ_OxRdtase_suE. [Graphical view]
Pfam	PF02508. Rnf-Nqr. 1 hit. [Graphical view]
PIRSF	PIRSF006102. NQR_DE. 1 hit.
TIGRFAMs	TIGR01940. nqrE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NQRE_HAEIN

Accession

Primary (citable) accession number: P71342

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	February 1, 1997
Last modified:	May 1, 2013
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program