P71335 (RRMF_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable rRNA maturation factor

Gene names

Ordered Locus Names:

HI_0004

Organism

Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

Taxonomic identifier

71421 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	154 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in rRNA and/or ribosome maturation and assembly. Required for normal 5' and 3' processing of 16S, 23S and 5S rRNAs. May have metal-dependent hydrolase activity By similarity. Ref.2
Cofactor	Binds 1 zinc ion.
Subcellular location	Cytoplasm By similarity HAMAP MF_00009.
Sequence similarities	Belongs to the rRNA maturation factor YbeY family.

Ontologies

Keywords
Biological process	Ribosome biogenesis rRNA processing
Cellular component	Cytoplasm
Ligand	Metal-binding Zinc
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	rRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 154

154

Probable rRNA maturation factor HAMAP MF_00009

PRO_0000102463

Sites

Metal binding

114

1

Zinc; catalytic By similarity

Metal binding

118

1

Zinc; catalytic By similarity

Metal binding

124

1

Zinc; catalytic By similarity

Secondary structure

1

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154

Helix Strand Turn

Details...

Beta strand

4 – 10

7

Helix

21 – 32

12

Beta strand

33 – 35

3

Beta strand

39 – 45

7

Helix

48 – 58

11

Beta strand

66 – 70

5

Beta strand

84 – 88

5

Helix

90 – 100

11

Helix

104 – 117

14

Turn

118 – 120

3

Turn

126 – 128

3

Helix

129 – 141

13

Turn

142 – 145

4

Sequences

	Sequence		Length	Mass (Da)
	P71335 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: C066F7AB7F9CA7AD	FASTA	154	17,355
	10 20 30 40 50 60 MGSVLVDLQI ATENIEGLPT EEQIVQWATG AVQPEGNEVE MTVRIVDEAE SHELNLTYRG 70 80 90 100 110 120 KDRPTNVLSF PFECPDEVEL PLLGDLVICR QVVEREASEQ EKPLMAHWAH MVVHGSLHLL 130 140 150 GYDHIEDDEA EEMESLETQI MQGLGFDDPY LAEK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed: 7542800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
[2]	"NMR structure of HI0004, a putative essential gene product from Haemophilus influenzae, and comparison with the X-ray structure of an Aquifex aeolicus homolog." Yeh D.C., Parsons L.M., Parsons J.F., Liu F., Eisenstein E., Orban J. Protein Sci. 14:424-430(2005) [PubMed: 15632286] [Abstract] Cited for: STRUCTURE BY NMR, FUNCTION, ZINC BINDING.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L42023 Genomic DNA. Translation: AAC21683.1.

PIR

A64140.

RefSeq

NP_438177.1. NC_000907.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1XAX	NMR	-	A	1-154	[»]

ProteinModelPortal

P71335.

SMR

P71335. Positions 3-146.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

950900.

GenomeReviews

Gene locus HI_0004 in contig L42023_GR.

KEGG

hin:HI0004.

NMPDR

fig|71421.1.peg.4.

PATRIC

20188459. VBIHaeInf48452_0004.

TIGR

HI_0004.

Phylogenomic databases

HOGENOM

HBG734136.

OMA

FTDPYKE.

ProtClustDB

PRK00016.

Enzyme and pathway databases

BioCyc

HINF71421:HI_0004-MONOMER.

Family and domain databases

HAMAP

MF_00009. rRNA_matur_factor.
[Tree]

InterPro

IPR023091. MetalPrtase_cat_dom_prd.
IPR020549. UPF0054_CS.
IPR002036. UPF0054_metalloprotease_prd.
[Graphical view]

Gene3D

G3DSA:3.40.390.30. MetalPrtase_cat_dom_prd. 1 hit.

KO

K07042.

Pfam

PF02130. UPF0054. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00043. TIGR00043. 1 hit.

PROSITE

PS01306. UPF0054. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

RRMF_HAEIN

Accession

Primary (citable) accession number: P71335

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	January 25, 2012
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program