ID   METF_PECCC              Reviewed;         298 AA.
AC   P71319;
DT   15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   16-JUN-2009, entry version 45.
DE   RecName: Full=5,10-methylenetetrahydrofolate reductase;
DE            EC=1.5.1.20;
GN   Name=metF;
OS   Pectobacterium carotovorum subsp. carotovorum (Erwinia carotovora
OS   subsp. carotovora).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Pectobacterium.
OX   NCBI_TaxID=555;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=71;
RA   Calcutt M.J., Lewis M.S., Eisenstark A.;
RL   Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY: 5-methyltetrahydrofolate + NAD(P)(+) = 5,10-
CC       methylenetetrahydrofolate + NAD(P)H.
CC   -!- COFACTOR: FAD (By similarity).
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate pathway.
CC   -!- SIMILARITY: Belongs to the methylenetetrahydrofolate reductase
CC       family.
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DR   EMBL; U74302; AAC72242.1; -; Genomic_DNA.
DR   HSSP; P00394; 1B5T.
DR   SMR; P71319; 3-294.
DR   BRENDA; 1.5.1.20; 290410.
DR   GO; GO:0004489; F:methylenetetrahydrofolate reductase (NADPH)...; IEA:EC.
DR   GO; GO:0009086; P:methionine biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR003171; Mehydrof_redctse.
DR   InterPro; IPR004620; MTHF_reductase_bac.
DR   Pfam; PF02219; MTHFR; 1.
DR   TIGRFAMs; TIGR00676; fadh2; 1.
PE   3: Inferred from homology;
KW   Amino-acid biosynthesis; FAD; Flavoprotein; Methionine biosynthesis;
KW   NAD; NADP; Oxidoreductase.
FT   CHAIN         1    298       5,10-methylenetetrahydrofolate reductase.
FT                                /FTId=PRO_0000190262.
SQ   SEQUENCE   298 AA;  33589 MW;  50FE729E4E3E2C56 CRC64;
     MSFFHANQRE ALNQSLAELQ GRINVSFEFF PPRTSDMEET LWSSIDRLSS LKPKFVSVTY
     GANSGERDRT HSIIKTIKER TGLEAAPHLT CIDASREQLR EIAQDYWESG IRHIVALRGD
     LPQEGGKPDM YAADLVSLLK EVGDFDISVA AYPEVHPEAK SAQADLINLK HKIDAGANRA
     ITQFFFDVES YLRFRDRCVA TGIDVEIVPG ILPVSNFKQL QKFATMTNVR VPNWMTTMFD
     GLDNDPETRK MVGASIAMDM VKILSREGVK DFHFYTLNRA ELSYAICHTL GVRPDVAR
//