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P71319

- METF_PECCC

UniProt

P71319 - METF_PECCC

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Protein
5,10-methylenetetrahydrofolate reductase
Gene
metF
Organism
Pectobacterium carotovorum subsp. carotovorum (Erwinia carotovora subsp. carotovora)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

Cofactori

FAD By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei28 – 281Proton donor/acceptor By similarity
Binding sitei88 – 881FAD By similarity
Binding sitei120 – 1201Substrate By similarity
Binding sitei152 – 1521FAD By similarity
Binding sitei183 – 1831Substrate By similarity
Binding sitei219 – 2191Substrate By similarity
Binding sitei223 – 2231Substrate By similarity
Binding sitei275 – 2751Substrate By similarity
Binding sitei279 – 2791Substrate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi28 – 336NAD By similarity
Nucleotide bindingi59 – 624FAD By similarity
Nucleotide bindingi59 – 602NAD By similarity
Nucleotide bindingi118 – 1203FAD By similarity
Nucleotide bindingi131 – 1322FAD By similarity
Nucleotide bindingi156 – 1594FAD By similarity
Nucleotide bindingi165 – 1728FAD By similarity

GO - Molecular functioni

  1. methylenetetrahydrofolate reductase (NAD(P)H) activity Source: UniProtKB-EC

GO - Biological processi

  1. methionine biosynthetic process Source: UniProtKB-KW
  2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

FAD, Flavoprotein, NAD, NADP

Enzyme and pathway databases

UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
5,10-methylenetetrahydrofolate reductase (EC:1.5.1.20)
Gene namesi
Name:metF
OrganismiPectobacterium carotovorum subsp. carotovorum (Erwinia carotovora subsp. carotovora)
Taxonomic identifieri555 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaePectobacterium

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2982985,10-methylenetetrahydrofolate reductase
PRO_0000190262Add
BLAST

Proteomic databases

PRIDEiP71319.

Structurei

3D structure databases

ProteinModelPortaliP71319.
SMRiP71319. Positions 3-294.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.20.20.220. 1 hit.
InterProiIPR029041. FAD-linked_oxidoreductase-like.
IPR003171. Mehydrof_redctse.
IPR004620. MTHF_reductase_bac.
[Graphical view]
PfamiPF02219. MTHFR. 1 hit.
[Graphical view]
SUPFAMiSSF51730. SSF51730. 1 hit.
TIGRFAMsiTIGR00676. fadh2. 1 hit.

Sequencei

Sequence statusi: Complete.

P71319-1 [UniParc]FASTAAdd to Basket

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MSFFHANQRE ALNQSLAELQ GRINVSFEFF PPRTSDMEET LWSSIDRLSS    50
LKPKFVSVTY GANSGERDRT HSIIKTIKER TGLEAAPHLT CIDASREQLR 100
EIAQDYWESG IRHIVALRGD LPQEGGKPDM YAADLVSLLK EVGDFDISVA 150
AYPEVHPEAK SAQADLINLK HKIDAGANRA ITQFFFDVES YLRFRDRCVA 200
TGIDVEIVPG ILPVSNFKQL QKFATMTNVR VPNWMTTMFD GLDNDPETRK 250
MVGASIAMDM VKILSREGVK DFHFYTLNRA ELSYAICHTL GVRPDVAR 298
Length:298
Mass (Da):33,589
Last modified:February 1, 1997 - v1
Checksum:i50FE729E4E3E2C56
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U74302 Genomic DNA. Translation: AAC72242.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U74302 Genomic DNA. Translation: AAC72242.1 .

3D structure databases

ProteinModelPortali P71319.
SMRi P71319. Positions 3-294.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi P71319.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00193 .

Family and domain databases

Gene3Di 3.20.20.220. 1 hit.
InterProi IPR029041. FAD-linked_oxidoreductase-like.
IPR003171. Mehydrof_redctse.
IPR004620. MTHF_reductase_bac.
[Graphical view ]
Pfami PF02219. MTHFR. 1 hit.
[Graphical view ]
SUPFAMi SSF51730. SSF51730. 1 hit.
TIGRFAMsi TIGR00676. fadh2. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Calcutt M.J., Lewis M.S., Eisenstark A.
    Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 71.

Entry informationi

Entry nameiMETF_PECCC
AccessioniPrimary (citable) accession number: P71319
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: February 1, 1997
Last modified: June 11, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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