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Protein

5,10-methylenetetrahydrofolate reductase

Gene

metF

Organism
Pectobacterium carotovorum subsp. carotovorum (Erwinia carotovora subsp. carotovora)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

Cofactori

FADBy similarity

Pathwayi: tetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei28Proton donor/acceptorBy similarity1
Binding sitei88FADBy similarity1
Binding sitei120SubstrateBy similarity1
Binding sitei152FADBy similarity1
Binding sitei183SubstrateBy similarity1
Binding sitei219SubstrateBy similarity1
Binding sitei223SubstrateBy similarity1
Binding sitei275SubstrateBy similarity1
Binding sitei279SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi28 – 33NADBy similarity6
Nucleotide bindingi59 – 62FADBy similarity4
Nucleotide bindingi59 – 60NADBy similarity2
Nucleotide bindingi118 – 120FADBy similarity3
Nucleotide bindingi131 – 132FADBy similarity2
Nucleotide bindingi156 – 159FADBy similarity4
Nucleotide bindingi165 – 172FADBy similarity8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

FAD, Flavoprotein, NAD, NADP

Enzyme and pathway databases

UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
5,10-methylenetetrahydrofolate reductase (EC:1.5.1.20)
Gene namesi
Name:metF
OrganismiPectobacterium carotovorum subsp. carotovorum (Erwinia carotovora subsp. carotovora)
Taxonomic identifieri555 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesPectobacteriaceaePectobacterium

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001902621 – 2985,10-methylenetetrahydrofolate reductaseAdd BLAST298

Proteomic databases

PRIDEiP71319.

Interactioni

Protein-protein interaction databases

STRINGi561230.PC1_0185.

Structurei

3D structure databases

ProteinModelPortaliP71319.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105SYT. Bacteria.
COG0685. LUCA.

Family and domain databases

CDDicd00537. MTHFR. 1 hit.
Gene3Di3.20.20.220. 1 hit.
InterProiIPR029041. FAD-linked_oxidoreductase-like.
IPR003171. Mehydrof_redctse.
IPR004620. MTHF_reductase_bac.
[Graphical view]
PfamiPF02219. MTHFR. 1 hit.
[Graphical view]
SUPFAMiSSF51730. SSF51730. 1 hit.
TIGRFAMsiTIGR00676. fadh2. 1 hit.

Sequencei

Sequence statusi: Complete.

P71319-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFFHANQRE ALNQSLAELQ GRINVSFEFF PPRTSDMEET LWSSIDRLSS
60 70 80 90 100
LKPKFVSVTY GANSGERDRT HSIIKTIKER TGLEAAPHLT CIDASREQLR
110 120 130 140 150
EIAQDYWESG IRHIVALRGD LPQEGGKPDM YAADLVSLLK EVGDFDISVA
160 170 180 190 200
AYPEVHPEAK SAQADLINLK HKIDAGANRA ITQFFFDVES YLRFRDRCVA
210 220 230 240 250
TGIDVEIVPG ILPVSNFKQL QKFATMTNVR VPNWMTTMFD GLDNDPETRK
260 270 280 290
MVGASIAMDM VKILSREGVK DFHFYTLNRA ELSYAICHTL GVRPDVAR
Length:298
Mass (Da):33,589
Last modified:February 1, 1997 - v1
Checksum:i50FE729E4E3E2C56
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U74302 Genomic DNA. Translation: AAC72242.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U74302 Genomic DNA. Translation: AAC72242.1.

3D structure databases

ProteinModelPortaliP71319.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi561230.PC1_0185.

Proteomic databases

PRIDEiP71319.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4105SYT. Bacteria.
COG0685. LUCA.

Enzyme and pathway databases

UniPathwayiUPA00193.

Family and domain databases

CDDicd00537. MTHFR. 1 hit.
Gene3Di3.20.20.220. 1 hit.
InterProiIPR029041. FAD-linked_oxidoreductase-like.
IPR003171. Mehydrof_redctse.
IPR004620. MTHF_reductase_bac.
[Graphical view]
PfamiPF02219. MTHFR. 1 hit.
[Graphical view]
SUPFAMiSSF51730. SSF51730. 1 hit.
TIGRFAMsiTIGR00676. fadh2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMETF_PECCC
AccessioniPrimary (citable) accession number: P71319
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: February 1, 1997
Last modified: November 2, 2016
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.