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P71319

- METF_PECCC

UniProt

P71319 - METF_PECCC

Protein

5,10-methylenetetrahydrofolate reductase

Gene

metF

Organism
Pectobacterium carotovorum subsp. carotovorum (Erwinia carotovora subsp. carotovora)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 63 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

    Cofactori

    FAD.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei28 – 281Proton donor/acceptorBy similarity
    Binding sitei88 – 881FADBy similarity
    Binding sitei120 – 1201SubstrateBy similarity
    Binding sitei152 – 1521FADBy similarity
    Binding sitei183 – 1831SubstrateBy similarity
    Binding sitei219 – 2191SubstrateBy similarity
    Binding sitei223 – 2231SubstrateBy similarity
    Binding sitei275 – 2751SubstrateBy similarity
    Binding sitei279 – 2791SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi28 – 336NADBy similarity
    Nucleotide bindingi59 – 624FADBy similarity
    Nucleotide bindingi59 – 602NADBy similarity
    Nucleotide bindingi118 – 1203FADBy similarity
    Nucleotide bindingi131 – 1322FADBy similarity
    Nucleotide bindingi156 – 1594FADBy similarity
    Nucleotide bindingi165 – 1728FADBy similarity

    GO - Molecular functioni

    1. methylenetetrahydrofolate reductase (NAD(P)H) activity Source: UniProtKB-EC

    GO - Biological processi

    1. methionine biosynthetic process Source: UniProtKB-KW
    2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Amino-acid biosynthesis, Methionine biosynthesis

    Keywords - Ligandi

    FAD, Flavoprotein, NAD, NADP

    Enzyme and pathway databases

    UniPathwayiUPA00193.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    5,10-methylenetetrahydrofolate reductase (EC:1.5.1.20)
    Gene namesi
    Name:metF
    OrganismiPectobacterium carotovorum subsp. carotovorum (Erwinia carotovora subsp. carotovora)
    Taxonomic identifieri555 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaePectobacterium

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 2982985,10-methylenetetrahydrofolate reductasePRO_0000190262Add
    BLAST

    Proteomic databases

    PRIDEiP71319.

    Structurei

    3D structure databases

    ProteinModelPortaliP71319.
    SMRiP71319. Positions 3-294.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    Gene3Di3.20.20.220. 1 hit.
    InterProiIPR029041. FAD-linked_oxidoreductase-like.
    IPR003171. Mehydrof_redctse.
    IPR004620. MTHF_reductase_bac.
    [Graphical view]
    PfamiPF02219. MTHFR. 1 hit.
    [Graphical view]
    SUPFAMiSSF51730. SSF51730. 1 hit.
    TIGRFAMsiTIGR00676. fadh2. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P71319-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSFFHANQRE ALNQSLAELQ GRINVSFEFF PPRTSDMEET LWSSIDRLSS    50
    LKPKFVSVTY GANSGERDRT HSIIKTIKER TGLEAAPHLT CIDASREQLR 100
    EIAQDYWESG IRHIVALRGD LPQEGGKPDM YAADLVSLLK EVGDFDISVA 150
    AYPEVHPEAK SAQADLINLK HKIDAGANRA ITQFFFDVES YLRFRDRCVA 200
    TGIDVEIVPG ILPVSNFKQL QKFATMTNVR VPNWMTTMFD GLDNDPETRK 250
    MVGASIAMDM VKILSREGVK DFHFYTLNRA ELSYAICHTL GVRPDVAR 298
    Length:298
    Mass (Da):33,589
    Last modified:February 1, 1997 - v1
    Checksum:i50FE729E4E3E2C56
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U74302 Genomic DNA. Translation: AAC72242.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U74302 Genomic DNA. Translation: AAC72242.1 .

    3D structure databases

    ProteinModelPortali P71319.
    SMRi P71319. Positions 3-294.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P71319.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00193 .

    Family and domain databases

    Gene3Di 3.20.20.220. 1 hit.
    InterProi IPR029041. FAD-linked_oxidoreductase-like.
    IPR003171. Mehydrof_redctse.
    IPR004620. MTHF_reductase_bac.
    [Graphical view ]
    Pfami PF02219. MTHFR. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51730. SSF51730. 1 hit.
    TIGRFAMsi TIGR00676. fadh2. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Calcutt M.J., Lewis M.S., Eisenstark A.
      Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 71.

    Entry informationi

    Entry nameiMETF_PECCC
    AccessioniPrimary (citable) accession number: P71319
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1999
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 63 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3