Reviewed, UniProtKB/Swiss-Prot P71319 (METF_PECCC)

Names and origin

Protein names

Recommended name:
    5,10-methylenetetrahydrofolate reductase
    EC=1.5.1.20

Gene names

Name:

metF

Organism

Pectobacterium carotovorum subsp. carotovorum (Erwinia carotovora subsp. carotovora)

Taxonomic identifier

555 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Pectobacterium

Protein attributes

Sequence length	298 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	5-methyltetrahydrofolate + NAD(P)(+) = 5,10-methylenetetrahydrofolate + NAD(P)H.
Cofactor	FAD By similarity.
Pathway	One-carbon metabolism; tetrahydrofolate pathway.
Sequence similarities	Belongs to the methylenetetrahydrofolate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Ligand	FAD Flavoprotein NAD NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	methylenetetrahydrofolate reductase (NADPH) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 298	298	5,10-methylenetetrahydrofolate reductase		PRO_0000190262

Sequences

	Sequence		Length	Mass (Da)
	P71319-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 50FE729E4E3E2C56	FASTA	298	33,589
	10 20 30 40 50 60 MSFFHANQRE ALNQSLAELQ GRINVSFEFF PPRTSDMEET LWSSIDRLSS LKPKFVSVTY 70 80 90 100 110 120 GANSGERDRT HSIIKTIKER TGLEAAPHLT CIDASREQLR EIAQDYWESG IRHIVALRGD 130 140 150 160 170 180 LPQEGGKPDM YAADLVSLLK EVGDFDISVA AYPEVHPEAK SAQADLINLK HKIDAGANRA 190 200 210 220 230 240 ITQFFFDVES YLRFRDRCVA TGIDVEIVPG ILPVSNFKQL QKFATMTNVR VPNWMTTMFD 250 260 270 280 290 GLDNDPETRK MVGASIAMDM VKILSREGVK DFHFYTLNRA ELSYAICHTL GVRPDVAR

References

[1]	Calcutt M.J., Lewis M.S., Eisenstark A. Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 71.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U74302 Genomic DNA. Translation: AAC72242.1.
3D structure databases
HSSP	HSSP built from PDB template 1B5T based on UniProtKB P00394.
SMR	P71319. Positions 3-294.
ModBase	Search...
Family and domain databases
InterPro	IPR003171. Mehydrof_redctse. IPR004620. MTHF_reductase_bac. [Graphical view]
Pfam	PF02219. MTHFR. 1 hit. [Graphical view]
TIGRFAMs	TIGR00676. fadh2. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METF_PECCC

Accession

Primary (citable) accession number: P71319

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	February 1, 1997
Last modified:	November 25, 2008
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)