Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P71319 (METF_PECCC) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
5,10-methylenetetrahydrofolate reductase

EC=1.5.1.20
Gene names
Name:metF
OrganismPectobacterium carotovorum subsp. carotovorum (Erwinia carotovora subsp. carotovora)
Taxonomic identifier555 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaePectobacterium

Protein attributes

Sequence length298 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

Cofactor

FAD By similarity.

Pathway

One-carbon metabolism; tetrahydrofolate interconversion.

Sequence similarities

Belongs to the methylenetetrahydrofolate reductase family.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Methionine biosynthesis
   LigandFAD
Flavoprotein
NAD
NADP
   Molecular functionOxidoreductase
Gene Ontology (GO)
   Biological_processmethionine biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

tetrahydrofolate interconversion

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytosol

Inferred from electronic annotation. Source: InterPro

   Molecular_functionmethylenetetrahydrofolate reductase (NAD(P)H) activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 2982985,10-methylenetetrahydrofolate reductase
PRO_0000190262

Regions

Nucleotide binding28 – 336NAD By similarity
Nucleotide binding59 – 624FAD By similarity
Nucleotide binding59 – 602NAD By similarity
Nucleotide binding118 – 1203FAD By similarity
Nucleotide binding131 – 1322FAD By similarity
Nucleotide binding156 – 1594FAD By similarity
Nucleotide binding165 – 1728FAD By similarity

Sites

Active site281Proton donor/acceptor By similarity
Binding site881FAD By similarity
Binding site1201Substrate By similarity
Binding site1521FAD By similarity
Binding site1831Substrate By similarity
Binding site2191Substrate By similarity
Binding site2231Substrate By similarity
Binding site2751Substrate By similarity
Binding site2791Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
P71319 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 50FE729E4E3E2C56

FASTA29833,589
        10         20         30         40         50         60 
MSFFHANQRE ALNQSLAELQ GRINVSFEFF PPRTSDMEET LWSSIDRLSS LKPKFVSVTY 

        70         80         90        100        110        120 
GANSGERDRT HSIIKTIKER TGLEAAPHLT CIDASREQLR EIAQDYWESG IRHIVALRGD 

       130        140        150        160        170        180 
LPQEGGKPDM YAADLVSLLK EVGDFDISVA AYPEVHPEAK SAQADLINLK HKIDAGANRA 

       190        200        210        220        230        240 
ITQFFFDVES YLRFRDRCVA TGIDVEIVPG ILPVSNFKQL QKFATMTNVR VPNWMTTMFD 

       250        260        270        280        290 
GLDNDPETRK MVGASIAMDM VKILSREGVK DFHFYTLNRA ELSYAICHTL GVRPDVAR 

« Hide

References

[1]Calcutt M.J., Lewis M.S., Eisenstark A.
Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 71.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U74302 Genomic DNA. Translation: AAC72242.1.

3D structure databases

ProteinModelPortalP71319.
SMRP71319. Positions 3-294.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEP71319.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00193.

Family and domain databases

Gene3D3.20.20.220. 1 hit.
InterProIPR029041. FAD-linked_oxidoreductase-like.
IPR003171. Mehydrof_redctse.
IPR004620. MTHF_reductase_bac.
[Graphical view]
PfamPF02219. MTHFR. 1 hit.
[Graphical view]
SUPFAMSSF51730. SSF51730. 1 hit.
TIGRFAMsTIGR00676. fadh2. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMETF_PECCC
AccessionPrimary (citable) accession number: P71319
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: February 1, 1997
Last modified: June 11, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways