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Protein

5,10-methylenetetrahydrofolate reductase

Gene

metF

Organism
Pectobacterium carotovorum subsp. carotovorum (Erwinia carotovora subsp. carotovora)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

Cofactori

FADBy similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei28 – 281Proton donor/acceptorBy similarity
Binding sitei88 – 881FADBy similarity
Binding sitei120 – 1201SubstrateBy similarity
Binding sitei152 – 1521FADBy similarity
Binding sitei183 – 1831SubstrateBy similarity
Binding sitei219 – 2191SubstrateBy similarity
Binding sitei223 – 2231SubstrateBy similarity
Binding sitei275 – 2751SubstrateBy similarity
Binding sitei279 – 2791SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi28 – 336NADBy similarity
Nucleotide bindingi59 – 624FADBy similarity
Nucleotide bindingi59 – 602NADBy similarity
Nucleotide bindingi118 – 1203FADBy similarity
Nucleotide bindingi131 – 1322FADBy similarity
Nucleotide bindingi156 – 1594FADBy similarity
Nucleotide bindingi165 – 1728FADBy similarity

GO - Molecular functioni

  1. methylenetetrahydrofolate reductase (NAD(P)H) activity Source: UniProtKB-EC

GO - Biological processi

  1. methionine biosynthetic process Source: UniProtKB-KW
  2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

FAD, Flavoprotein, NAD, NADP

Enzyme and pathway databases

UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
5,10-methylenetetrahydrofolate reductase (EC:1.5.1.20)
Gene namesi
Name:metF
OrganismiPectobacterium carotovorum subsp. carotovorum (Erwinia carotovora subsp. carotovora)
Taxonomic identifieri555 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaePectobacterium

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2982985,10-methylenetetrahydrofolate reductasePRO_0000190262Add
BLAST

Proteomic databases

PRIDEiP71319.

Structurei

3D structure databases

ProteinModelPortaliP71319.
SMRiP71319. Positions 3-294.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.20.20.220. 1 hit.
InterProiIPR029041. FAD-linked_oxidoreductase-like.
IPR003171. Mehydrof_redctse.
IPR004620. MTHF_reductase_bac.
[Graphical view]
PfamiPF02219. MTHFR. 1 hit.
[Graphical view]
SUPFAMiSSF51730. SSF51730. 1 hit.
TIGRFAMsiTIGR00676. fadh2. 1 hit.

Sequencei

Sequence statusi: Complete.

P71319-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSFFHANQRE ALNQSLAELQ GRINVSFEFF PPRTSDMEET LWSSIDRLSS
60 70 80 90 100
LKPKFVSVTY GANSGERDRT HSIIKTIKER TGLEAAPHLT CIDASREQLR
110 120 130 140 150
EIAQDYWESG IRHIVALRGD LPQEGGKPDM YAADLVSLLK EVGDFDISVA
160 170 180 190 200
AYPEVHPEAK SAQADLINLK HKIDAGANRA ITQFFFDVES YLRFRDRCVA
210 220 230 240 250
TGIDVEIVPG ILPVSNFKQL QKFATMTNVR VPNWMTTMFD GLDNDPETRK
260 270 280 290
MVGASIAMDM VKILSREGVK DFHFYTLNRA ELSYAICHTL GVRPDVAR
Length:298
Mass (Da):33,589
Last modified:February 1, 1997 - v1
Checksum:i50FE729E4E3E2C56
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U74302 Genomic DNA. Translation: AAC72242.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U74302 Genomic DNA. Translation: AAC72242.1.

3D structure databases

ProteinModelPortaliP71319.
SMRiP71319. Positions 3-294.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP71319.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00193.

Family and domain databases

Gene3Di3.20.20.220. 1 hit.
InterProiIPR029041. FAD-linked_oxidoreductase-like.
IPR003171. Mehydrof_redctse.
IPR004620. MTHF_reductase_bac.
[Graphical view]
PfamiPF02219. MTHFR. 1 hit.
[Graphical view]
SUPFAMiSSF51730. SSF51730. 1 hit.
TIGRFAMsiTIGR00676. fadh2. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Calcutt M.J., Lewis M.S., Eisenstark A.
    Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 71.

Entry informationi

Entry nameiMETF_PECCC
AccessioniPrimary (citable) accession number: P71319
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: February 1, 1997
Last modified: November 26, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.