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Protein

HTH-type transcriptional regulator EcpR

Gene

ecpR

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Part of the ecpRABCDE operon, which encodes the E.coli common pilus (ECP). ECP is found in both commensal and pathogenic strains and plays a dual role in early-stage biofilm development and host cell recognition. Positively regulates the expression of the ecp operon (By similarity). Also represses expression of the flagellar master operon flhDC, and consequently prevents flagellum biosynthesis and motility. Acts by binding to the regulatory region of the flhDC operon (Probable).By similarityCurated1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi162 – 18120H-T-H motifPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:G6165-MONOMER.
ECOL316407:JW5031-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
HTH-type transcriptional regulator EcpR
Gene namesi
Name:ecpR
Synonyms:matA, ykgK
Ordered Locus Names:b0294, JW5031
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14324. ecpR.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi179 – 1791H → P: Slightly increases motility. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 196196HTH-type transcriptional regulator EcpRPRO_0000184199Add
BLAST

Proteomic databases

PaxDbiP71301.
PRIDEiP71301.

Expressioni

Inductioni

Negatively regulated by H-NS. Positively autoregulated. Also positively regulated by IHF (By similarity).By similarity

Interactioni

Protein-protein interaction databases

BioGridi4259793. 6 interactions.
IntActiP71301. 1 interaction.
STRINGi511145.b0294.

Structurei

3D structure databases

ProteinModelPortaliP71301.
SMRiP71301. Positions 144-188.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini138 – 19659HTH luxR-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the EcpR/MatA family.Curated
Contains 1 HTH luxR-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4106JB4. Bacteria.
ENOG410Y9ZI. LUCA.
HOGENOMiHOG000059412.
OMAiCIWPAHD.
OrthoDBiEOG69GZGV.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR016032. Sig_transdc_resp-reg_C-effctor.
IPR000792. Tscrpt_reg_LuxR_C.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00196. GerE. 1 hit.
[Graphical view]
PRINTSiPR00038. HTHLUXR.
SMARTiSM00421. HTH_LUXR. 1 hit.
[Graphical view]
SUPFAMiSSF46894. SSF46894. 1 hit.
PROSITEiPS50043. HTH_LUXR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P71301-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTWQSDYSRD YEVKNHMECQ NRSDKYIWSP HDAYFYKGLS ELIVDIDRLI
60 70 80 90 100
YLSLEKIRKD FVFINLSTDS LSEFINRDNE WLSAVKGKQV VLIAARKSEA
110 120 130 140 150
LANYWYYNSN IRGVVYAGLS RDIRKELVYV INGRFLRKDI KKDKITDREM
160 170 180 190
EIIRMTAQGM QPKSIARIEN CSVKTVYTHR RNAEAKLYSK IYKLVQ
Length:196
Mass (Da):23,274
Last modified:February 1, 1997 - v1
Checksum:i5A3676E17E9793B9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73857 Genomic DNA. Translation: AAB18023.1.
U00096 Genomic DNA. Translation: AAC73397.1.
AP009048 Genomic DNA. Translation: BAE76078.1.
PIRiF64755.
RefSeqiNP_414828.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAC73397; AAC73397; b0294.
BAE76078; BAE76078; BAE76078.
GeneIDi944966.
KEGGiecj:JW5031.
eco:b0294.
PATRICi32115711. VBIEscCol129921_0298.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73857 Genomic DNA. Translation: AAB18023.1.
U00096 Genomic DNA. Translation: AAC73397.1.
AP009048 Genomic DNA. Translation: BAE76078.1.
PIRiF64755.
RefSeqiNP_414828.1. NC_000913.3.

3D structure databases

ProteinModelPortaliP71301.
SMRiP71301. Positions 144-188.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259793. 6 interactions.
IntActiP71301. 1 interaction.
STRINGi511145.b0294.

Proteomic databases

PaxDbiP71301.
PRIDEiP71301.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73397; AAC73397; b0294.
BAE76078; BAE76078; BAE76078.
GeneIDi944966.
KEGGiecj:JW5031.
eco:b0294.
PATRICi32115711. VBIEscCol129921_0298.

Organism-specific databases

EchoBASEiEB4070.
EcoGeneiEG14324. ecpR.

Phylogenomic databases

eggNOGiENOG4106JB4. Bacteria.
ENOG410Y9ZI. LUCA.
HOGENOMiHOG000059412.
OMAiCIWPAHD.
OrthoDBiEOG69GZGV.

Enzyme and pathway databases

BioCyciEcoCyc:G6165-MONOMER.
ECOL316407:JW5031-MONOMER.

Miscellaneous databases

PROiP71301.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR016032. Sig_transdc_resp-reg_C-effctor.
IPR000792. Tscrpt_reg_LuxR_C.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00196. GerE. 1 hit.
[Graphical view]
PRINTSiPR00038. HTHLUXR.
SMARTiSM00421. HTH_LUXR. 1 hit.
[Graphical view]
SUPFAMiSSF46894. SSF46894. 1 hit.
PROSITEiPS50043. HTH_LUXR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence of minutes 4-25 of Escherichia coli."
    Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "matB, a common fimbrillin gene of Escherichia coli, expressed in a genetically conserved, virulent clonal group."
    Pouttu R., Westerlund-Wikstrom B., Lang H., Alsti K., Virkola R., Saarela U., Siitonen A., Kalkkinen N., Korhonen T.K.
    J. Bacteriol. 183:4727-4736(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: EXPRESSION.
    Strain: K12.
  5. "The fimbriae activator MatA switches off motility in Escherichia coli by repression of the flagellar master operon flhDC."
    Lehti T.A., Bauchart P., Dobrindt U., Korhonen T.K., Westerlund-Wikstrom B.
    Microbiology 158:1444-1455(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF HIS-179.
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiECPR_ECOLI
AccessioniPrimary (citable) accession number: P71301
Secondary accession number(s): Q2MCC8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 1, 1997
Last modified: January 20, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Expressed in strain K12.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.