P71298 (INTF_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative prophage CP4-6 integrase

Gene names

Name:	intF
Synonyms:	yagO
Ordered Locus Names:	b0281, JW0275

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	466 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome.
Sequence similarities	Belongs to the 'phage' integrase family.

Ontologies

Keywords
Biological process	DNA integration DNA recombination Viral genome integration Virus entry into host cell
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA integration Inferred from electronic annotation. Source: UniProtKB-KW DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW establishment of integrated proviral latency Inferred from electronic annotation. Source: UniProtKB-KW viral entry into host cell Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 466	466	Putative prophage CP4-6 integrase		PRO_0000197515
Sites
	Active site	432	1	O-(3'-phospho-DNA)-tyrosine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P71298 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 4876E3F1898CE465	FASTA	466	53,074
	10 20 30 40 50 60 MFIPSIYLHQ QLHYCKTAIL NWSRKMALSR QKFTFERLRR FTLPEGKKQT FLWDADVTTL 70 80 90 100 110 120 ACRATSGAKA FVFQSVYAGK TLRMTIGNIN DWKIDDARAE ARRLQTLIDT GIDPRIAKAV 130 140 150 160 170 180 KIAEAESLQA ESRKTKVTFS VAWEDYLQEL RTGISAKTKR PYSTRYIADH INLSSRGGES 190 200 210 220 230 240 KKRGQGPTSA GPLASLLNLP LSELTPDYIA AWLSTERQNR PTVTAHAYRL LRAFIKWSNY 250 260 270 280 290 300 QKKYQGIIPG DLAQDYNVRK MVPVSASKAD DCLQKEQLKS WFSAVRSLNN PIASAYLQVL 310 320 330 340 350 360 LLTGARREEI ASLRWSDVDF KWSSMRIKDK IEGERIIPLT PYVSELLNVL AQSPNSDVNK 370 380 390 400 410 420 EGWVFRSNSK SGKIIEPRSA HNRALVLAEL PHISLHGLRR SFGTLAEWVE VPTGIVAQIM 430 440 450 460 GHKPSALAEK HYRRRPLDLL RKWHEKIETW ILNEAGITIK NNVDMR

References

[1]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U73857 Genomic DNA. Translation: AAB18010.1. U00096 Genomic DNA. Translation: AAC73384.1. AP009048 Genomic DNA. Translation: BAE76065.1.
PIR	A64754.
RefSeq	NP_414815.1. NC_000913.2. YP_488577.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P71298.
ModBase	Search...
Protein-protein interaction databases
IntAct	P71298. 1 interaction.
STRING	511145.b0281.
Proteomic databases
PaxDb	P71298.
PRIDE	P71298.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC73384; AAC73384; b0281. BAE76065; BAE76065; BAE76065.
GeneID	12930790. 947351.
KEGG	ecj:Y75_p0273. eco:b0281.
PATRIC	32115683. VBIEscCol129921_0285.
Organism-specific databases
EchoBASE	EB3324.
EcoGene	EG13554. intF.
Phylogenomic databases
eggNOG	COG0582.
HOGENOM	HOG000258764.
OMA	YRIERRK.
ProtClustDB	CLSK891713.
Enzyme and pathway databases
BioCyc	EcoCyc:G6152-MONOMER. ECOL316407:JW0275-MONOMER.
Gene expression databases
Genevestigator	P71298.
Family and domain databases
Gene3D	1.10.443.10. 1 hit.
InterPro	IPR011010. DNA_brk_join_enz. IPR025166. DUF4102. IPR013762. Integrase-like_cat-core. IPR002104. Integrase_catalytic. [Graphical view]
Pfam	PF13356. DUF4102. 1 hit. PF00589. Phage_integrase. 1 hit. [Graphical view]
SUPFAM	SSF56349. DNA_brk_join_enz. 1 hit.
ProtoNet	Search...

Entry information

Entry name

INTF_ECOLI

Accession

Primary (citable) accession number: P71298
Secondary accession number(s): Q2MCE1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	February 1, 1997
Last modified:	May 1, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program