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Protein

Putative prophage CP4-6 integrase

Gene

intF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei432 – 4321O-(3'-phospho-DNA)-tyrosine intermediateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

DNA integration, DNA recombination, Viral genome integration, Virus entry into host cell

Enzyme and pathway databases

BioCyciEcoCyc:G6152-MONOMER.
ECOL316407:JW0275-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative prophage CP4-6 integrase
Gene namesi
Name:intF
Synonyms:yagO
Ordered Locus Names:b0281, JW0275
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13554. intF.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 466466Putative prophage CP4-6 integrasePRO_0000197515Add
BLAST

Proteomic databases

PaxDbiP71298.
PRIDEiP71298.

Interactioni

Protein-protein interaction databases

BioGridi4259784. 10 interactions.
IntActiP71298. 1 interaction.
STRINGi511145.b0281.

Structurei

3D structure databases

ProteinModelPortaliP71298.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 'phage' integrase family.Curated

Phylogenomic databases

eggNOGiENOG41067CV. Bacteria.
COG0582. LUCA.
HOGENOMiHOG000258764.
InParanoidiP71298.
OMAiDFRWRSL.

Family and domain databases

Gene3Di1.10.443.10. 1 hit.
InterProiIPR011010. DNA_brk_join_enz.
IPR025166. DUF4102.
IPR013762. Integrase-like_cat.
IPR002104. Integrase_catalytic.
[Graphical view]
PfamiPF13356. DUF4102. 1 hit.
PF00589. Phage_integrase. 1 hit.
[Graphical view]
SUPFAMiSSF56349. SSF56349. 1 hit.

Sequencei

Sequence statusi: Complete.

P71298-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFIPSIYLHQ QLHYCKTAIL NWSRKMALSR QKFTFERLRR FTLPEGKKQT
60 70 80 90 100
FLWDADVTTL ACRATSGAKA FVFQSVYAGK TLRMTIGNIN DWKIDDARAE
110 120 130 140 150
ARRLQTLIDT GIDPRIAKAV KIAEAESLQA ESRKTKVTFS VAWEDYLQEL
160 170 180 190 200
RTGISAKTKR PYSTRYIADH INLSSRGGES KKRGQGPTSA GPLASLLNLP
210 220 230 240 250
LSELTPDYIA AWLSTERQNR PTVTAHAYRL LRAFIKWSNY QKKYQGIIPG
260 270 280 290 300
DLAQDYNVRK MVPVSASKAD DCLQKEQLKS WFSAVRSLNN PIASAYLQVL
310 320 330 340 350
LLTGARREEI ASLRWSDVDF KWSSMRIKDK IEGERIIPLT PYVSELLNVL
360 370 380 390 400
AQSPNSDVNK EGWVFRSNSK SGKIIEPRSA HNRALVLAEL PHISLHGLRR
410 420 430 440 450
SFGTLAEWVE VPTGIVAQIM GHKPSALAEK HYRRRPLDLL RKWHEKIETW
460
ILNEAGITIK NNVDMR
Length:466
Mass (Da):53,074
Last modified:February 1, 1997 - v1
Checksum:i4876E3F1898CE465
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73857 Genomic DNA. Translation: AAB18010.1.
U00096 Genomic DNA. Translation: AAC73384.1.
AP009048 Genomic DNA. Translation: BAE76065.1.
PIRiA64754.
RefSeqiNP_414815.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAC73384; AAC73384; b0281.
BAE76065; BAE76065; BAE76065.
GeneIDi947351.
KEGGiecj:JW0275.
eco:b0281.
PATRICi32115683. VBIEscCol129921_0285.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73857 Genomic DNA. Translation: AAB18010.1.
U00096 Genomic DNA. Translation: AAC73384.1.
AP009048 Genomic DNA. Translation: BAE76065.1.
PIRiA64754.
RefSeqiNP_414815.1. NC_000913.3.

3D structure databases

ProteinModelPortaliP71298.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259784. 10 interactions.
IntActiP71298. 1 interaction.
STRINGi511145.b0281.

Proteomic databases

PaxDbiP71298.
PRIDEiP71298.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73384; AAC73384; b0281.
BAE76065; BAE76065; BAE76065.
GeneIDi947351.
KEGGiecj:JW0275.
eco:b0281.
PATRICi32115683. VBIEscCol129921_0285.

Organism-specific databases

EchoBASEiEB3324.
EcoGeneiEG13554. intF.

Phylogenomic databases

eggNOGiENOG41067CV. Bacteria.
COG0582. LUCA.
HOGENOMiHOG000258764.
InParanoidiP71298.
OMAiDFRWRSL.

Enzyme and pathway databases

BioCyciEcoCyc:G6152-MONOMER.
ECOL316407:JW0275-MONOMER.

Miscellaneous databases

PROiP71298.

Family and domain databases

Gene3Di1.10.443.10. 1 hit.
InterProiIPR011010. DNA_brk_join_enz.
IPR025166. DUF4102.
IPR013762. Integrase-like_cat.
IPR002104. Integrase_catalytic.
[Graphical view]
PfamiPF13356. DUF4102. 1 hit.
PF00589. Phage_integrase. 1 hit.
[Graphical view]
SUPFAMiSSF56349. SSF56349. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiINTF_ECOLI
AccessioniPrimary (citable) accession number: P71298
Secondary accession number(s): Q2MCE1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 1, 1997
Last modified: September 7, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.