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Protein

Colanic acid biosynthesis protein WcaM

Gene

wcaM

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Pathwayi: slime polysaccharide biosynthesis

This protein is involved in the pathway slime polysaccharide biosynthesis, which is part of Slime biogenesis.
View all proteins of this organism that are known to be involved in the pathway slime polysaccharide biosynthesis and in Slime biogenesis.

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Lipopolysaccharide biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:G7094-MONOMER.
ECOL316407:JW2028-MONOMER.
UniPathwayiUPA00936.

Names & Taxonomyi

Protein namesi
Recommended name:
Colanic acid biosynthesis protein WcaM
Gene namesi
Name:wcaM
Synonyms:yefK
Ordered Locus Names:b2043, JW2028
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12651. wcaM.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2828Tat-type signalSequence analysisAdd
BLAST
Chaini29 – 464436Colanic acid biosynthesis protein WcaMPRO_0000065958Add
BLAST

Post-translational modificationi

Exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Proteomic databases

PaxDbiP71244.
PRIDEiP71244.

Interactioni

Protein-protein interaction databases

BioGridi4259677. 196 interactions.
IntActiP71244. 4 interactions.
STRINGi511145.b2043.

Structurei

3D structure databases

ProteinModelPortaliP71244.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105G1A. Bacteria.
ENOG4110WUJ. LUCA.
HOGENOMiHOG000121919.
KOiK16711.
OMAiVRGKFMA.
OrthoDBiEOG66XB8Z.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR023882. Colanic_acid_synth_WcaM.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
TIGRFAMsiTIGR04004. WcaM. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P71244-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPFKKLSRRT FLTASSALAF LHTPFARALP ARQSVNINDY NPHDWIASFK
60 70 80 90 100
QAFSEGQTVV VPAGLVCDNI NTGIFIPPGK TLHILGSLRG NGRGRFVLQD
110 120 130 140 150
GSQVTGEDGG SMHNITLDVR GSDCTIKGLT MSGFGPVTQI YIGGKNKRVM
160 170 180 190 200
RNLIIDNLTV SHANYAILRQ GFHNQIIGAN ITNCKFSDLQ GDAIEWNVAI
210 220 230 240 250
NDRDILISDH VIERINCTNG KINWGIGIGL AGSTYDNNYP EDQAVKNFVV
260 270 280 290 300
ANITGSDCRQ LIHVENGKHF VIRNIKARNI TPDFSKKAGI DNATVAIYGC
310 320 330 340 350
DNFVIDNIEM INSAGMLIGY GVIKGKYLSI PQNFRVNDIQ LDNTHLAYKL
360 370 380 390 400
RGIQISAGNA VSFVALTNIE MKRASLELHN KPQHFFMRNI NVMQESSVGP
410 420 430 440 450
ALSMNFDMRK DVRGVFMAKK ETLLSLANVH AVNEKGQSSV DIDRINHHIV
460
NVEKINFRLP ELRE
Length:464
Mass (Da):51,316
Last modified:November 1, 1997 - v2
Checksum:i72A7655DC07368BE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U38473 Genomic DNA. Translation: AAC77852.1.
U00096 Genomic DNA. Translation: AAC75104.1.
AP009048 Genomic DNA. Translation: BAA15897.1.
PIRiB64970.
RefSeqiNP_416547.1. NC_000913.3.
WP_001116026.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75104; AAC75104; b2043.
BAA15897; BAA15897; BAA15897.
GeneIDi946561.
KEGGiecj:JW2028.
eco:b2043.
PATRICi32119419. VBIEscCol129921_2120.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U38473 Genomic DNA. Translation: AAC77852.1.
U00096 Genomic DNA. Translation: AAC75104.1.
AP009048 Genomic DNA. Translation: BAA15897.1.
PIRiB64970.
RefSeqiNP_416547.1. NC_000913.3.
WP_001116026.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP71244.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259677. 196 interactions.
IntActiP71244. 4 interactions.
STRINGi511145.b2043.

Proteomic databases

PaxDbiP71244.
PRIDEiP71244.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75104; AAC75104; b2043.
BAA15897; BAA15897; BAA15897.
GeneIDi946561.
KEGGiecj:JW2028.
eco:b2043.
PATRICi32119419. VBIEscCol129921_2120.

Organism-specific databases

EchoBASEiEB2521.
EcoGeneiEG12651. wcaM.

Phylogenomic databases

eggNOGiENOG4105G1A. Bacteria.
ENOG4110WUJ. LUCA.
HOGENOMiHOG000121919.
KOiK16711.
OMAiVRGKFMA.
OrthoDBiEOG66XB8Z.

Enzyme and pathway databases

UniPathwayiUPA00936.
BioCyciEcoCyc:G7094-MONOMER.
ECOL316407:JW2028-MONOMER.

Miscellaneous databases

PROiP71244.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR023882. Colanic_acid_synth_WcaM.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
TIGRFAMsiTIGR04004. WcaM. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Organization of the Escherichia coli K-12 gene cluster responsible for production of the extracellular polysaccharide colanic acid."
    Stevenson G., Andrianopoulos K., Hobbs M., Reeves P.R.
    J. Bacteriol. 178:4885-4893(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. Reeves P.R.
    Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
    Strain: K12.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Export pathway selectivity of Escherichia coli twin arginine translocation signal peptides."
    Tullman-Ercek D., DeLisa M.P., Kawarasaki Y., Iranpour P., Ribnicky B., Palmer T., Georgiou G.
    J. Biol. Chem. 282:8309-8316(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: EXPORT VIA THE TAT-SYSTEM.

Entry informationi

Entry nameiWCAM_ECOLI
AccessioniPrimary (citable) accession number: P71244
Secondary accession number(s): P76378
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: January 20, 2016
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.