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Protein

Putative colanic acid biosynthesis glycosyltransferase WcaL

Gene

wcaL

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Pathwayi: slime polysaccharide biosynthesis

This protein is involved in the pathway slime polysaccharide biosynthesis, which is part of Slime biogenesis.
View all proteins of this organism that are known to be involved in the pathway slime polysaccharide biosynthesis and in Slime biogenesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Lipopolysaccharide biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:G7095-MONOMER.
ECOL316407:JW2029-MONOMER.
UniPathwayiUPA00936.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative colanic acid biosynthesis glycosyltransferase WcaL (EC:2.4.-.-)
Gene namesi
Name:wcaL
Synonyms:yefL
Ordered Locus Names:b2044, JW2029
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12652. wcaL.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 406406Putative colanic acid biosynthesis glycosyltransferase WcaLPRO_0000080311Add
BLAST

Proteomic databases

PaxDbiP71243.
PRIDEiP71243.

Interactioni

Protein-protein interaction databases

BioGridi4259676. 168 interactions.
DIPiDIP-11127N.
IntActiP71243. 1 interaction.
MINTiMINT-1263848.
STRINGi511145.b2044.

Structurei

3D structure databases

ProteinModelPortaliP71243.
SMRiP71243. Positions 103-405.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4107T8Q. Bacteria.
COG0438. LUCA.
HOGENOMiHOG000121940.
InParanoidiP71243.
KOiK16703.
OMAiYGDEARN.
OrthoDBiEOG6XM7CJ.
PhylomeDBiP71243.

Family and domain databases

InterProiIPR023884. Colanic_acid_synth_WcaL.
IPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR04005. wcaL. 1 hit.

Sequencei

Sequence statusi: Complete.

P71243-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVGFFLLKF PLSSETFVLN QITAFIDMGF EVEILALQKG DTQNTHAAWT
60 70 80 90 100
KYNLAARTRW LQDEPTGKVA KLRHRASQTL RGIHRKNTWQ ALNLKRYGAE
110 120 130 140 150
SRNLILSAIC GQVATPFRAD VFIAHFGPAG VTAAKLRELG VIRGKIATIF
160 170 180 190 200
HGIDISSREV LNHYTPEYQQ LFRRGDLMLP ISDLWAGRLQ KMGCPREKIA
210 220 230 240 250
VSRMGVDMTR FSPRPVKAPA TPLEIISVAR LTEKKGLHVA IEACRQLKEQ
260 270 280 290 300
GVAFRYRILG IGPWERRLRT LIEQYQLEDV VEMPGFKPSH EVKAMLDDAD
310 320 330 340 350
VFLLPSVTGA DGDMEGIPVA LMEAMAVGIP VVSTLHSGIP ELVEADKSGW
360 370 380 390 400
LVPENDARAL AQRLAAFSQL DTDELAPVVK RAREKVEHDF NQQVINRELA

SLLQAL
Length:406
Mass (Da):45,362
Last modified:November 1, 1997 - v2
Checksum:iEBC64DB398677D65
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti126 – 1261F → Y in AAC77851 (Ref. 2) Curated
Sequence conflicti129 – 1291A → T in AAC77851 (Ref. 2) Curated
Sequence conflicti144 – 1441G → A in AAC77851 (Ref. 2) Curated
Sequence conflicti205 – 2051G → A in AAC77851 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U38473 Genomic DNA. Translation: AAC77851.1.
U00096 Genomic DNA. Translation: AAC75105.1.
AP009048 Genomic DNA. Translation: BAA15898.1.
PIRiC64970.
RefSeqiNP_416548.1. NC_000913.3.
WP_000862592.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75105; AAC75105; b2044.
BAA15898; BAA15898; BAA15898.
GeneIDi946565.
KEGGiecj:JW2029.
eco:b2044.
PATRICi32119421. VBIEscCol129921_2121.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U38473 Genomic DNA. Translation: AAC77851.1.
U00096 Genomic DNA. Translation: AAC75105.1.
AP009048 Genomic DNA. Translation: BAA15898.1.
PIRiC64970.
RefSeqiNP_416548.1. NC_000913.3.
WP_000862592.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP71243.
SMRiP71243. Positions 103-405.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259676. 168 interactions.
DIPiDIP-11127N.
IntActiP71243. 1 interaction.
MINTiMINT-1263848.
STRINGi511145.b2044.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Proteomic databases

PaxDbiP71243.
PRIDEiP71243.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75105; AAC75105; b2044.
BAA15898; BAA15898; BAA15898.
GeneIDi946565.
KEGGiecj:JW2029.
eco:b2044.
PATRICi32119421. VBIEscCol129921_2121.

Organism-specific databases

EchoBASEiEB2522.
EcoGeneiEG12652. wcaL.

Phylogenomic databases

eggNOGiENOG4107T8Q. Bacteria.
COG0438. LUCA.
HOGENOMiHOG000121940.
InParanoidiP71243.
KOiK16703.
OMAiYGDEARN.
OrthoDBiEOG6XM7CJ.
PhylomeDBiP71243.

Enzyme and pathway databases

UniPathwayiUPA00936.
BioCyciEcoCyc:G7095-MONOMER.
ECOL316407:JW2029-MONOMER.

Miscellaneous databases

PROiP71243.

Family and domain databases

InterProiIPR023884. Colanic_acid_synth_WcaL.
IPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR04005. wcaL. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Organization of the Escherichia coli K-12 gene cluster responsible for production of the extracellular polysaccharide colanic acid."
    Stevenson G., Andrianopoulos K., Hobbs M., Reeves P.R.
    J. Bacteriol. 178:4885-4893(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. Reeves P.R.
    Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
    Strain: K12.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiWCAL_ECOLI
AccessioniPrimary (citable) accession number: P71243
Secondary accession number(s): P76379
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: May 11, 2016
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.