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Protein

UDP-glucose:undecaprenyl-phosphate glucose-1-phosphate transferase

Gene

wcaJ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Is the initiating enzyme for colanic acid (CA) synthesis. Catalyzes the transfer of the glucose-1-phosphate moiety from UDP-Glc onto the carrier lipid undecaprenyl phosphate (C55-P), forming a phosphoanhydride bond yielding to glucosyl-pyrophosphoryl-undecaprenol (Glc-PP-C55). Also possesses a weak galactose-1-P transferase activity.1 Publication

Miscellaneous

Complements holdfast synthesis in the C.crescentus mutant lacking hfsE, pssY and pssZ genes. Can also partially complement the O-antigen defect in the S.typhimurium wbaP deletion strain (PubMed:22408159).1 Publication

Catalytic activityi

UDP-glucose + ditrans,octacis-undecaprenyl phosphate = UMP + alpha-D-glucopyranosyl-diphospho-ditrans,octacis-undecaprenol.1 Publication

Pathwayi: colanic acid biosynthesis

This protein is involved in the pathway colanic acid biosynthesis, which is part of Exopolysaccharide biosynthesis.1 Publication
View all proteins of this organism that are known to be involved in the pathway colanic acid biosynthesis and in Exopolysaccharide biosynthesis.

GO - Molecular functioni

GO - Biological processi

  • colanic acid biosynthetic process Source: EcoCyc
  • lipopolysaccharide biosynthetic process Source: UniProtKB-KW

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processExopolysaccharide synthesis, Lipopolysaccharide biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:G7098-MONOMER
MetaCyc:G7098-MONOMER
UniPathwayiUPA00980

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-glucose:undecaprenyl-phosphate glucose-1-phosphate transferase (EC:2.7.8.31)
Short name:
UDP-Glc:Und-P Glc-1-P transferase
Alternative name(s):
Colanic acid biosynthesis UDP-glucose lipid carrier transferase
Glucosyl-P-P-undecaprenol synthase
Gene namesi
Name:wcaJ
Ordered Locus Names:b2047, JW2032
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13575 wcaJ

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 15CytoplasmicSequence analysisAdd BLAST15
Transmembranei16 – 36HelicalSequence analysisAdd BLAST21
Topological domaini37 – 38PeriplasmicSequence analysis2
Transmembranei39 – 59HelicalSequence analysisAdd BLAST21
Topological domaini60 – 80CytoplasmicSequence analysisAdd BLAST21
Transmembranei81 – 101HelicalSequence analysisAdd BLAST21
Topological domaini102 – 104PeriplasmicSequence analysis3
Transmembranei105 – 125HelicalSequence analysisAdd BLAST21
Topological domaini126 – 278CytoplasmicSequence analysisAdd BLAST153
Transmembranei279 – 299HelicalSequence analysisAdd BLAST21
Topological domaini300 – 464PeriplasmicSequence analysisAdd BLAST165

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene show a non-mucoid phenotype.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001664701 – 464UDP-glucose:undecaprenyl-phosphate glucose-1-phosphate transferaseAdd BLAST464

Proteomic databases

PaxDbiP71241
PRIDEiP71241

Interactioni

Protein-protein interaction databases

BioGridi4263311, 198 interactors
DIPiDIP-11125N
IntActiP71241, 1 interactor
STRINGi316385.ECDH10B_2197

Structurei

3D structure databases

ProteinModelPortaliP71241
SMRiP71241
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the bacterial sugar transferase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105D3Q Bacteria
COG2148 LUCA
HOGENOMiHOG000153662
InParanoidiP71241
KOiK03606
OMAiRPRAYSY
PhylomeDBiP71241

Family and domain databases

InterProiView protein in InterPro
IPR003362 Bact_transf
IPR017475 EPS_sugar_tfrase
IPR017473 Undecaprenyl-P_gluc_Ptfrase
PfamiView protein in Pfam
PF02397 Bac_transf, 1 hit
TIGRFAMsiTIGR03025 EPS_sugtrans, 1 hit
TIGR03023 WcaJ_sugtrans, 1 hit

Sequencei

Sequence statusi: Complete.

P71241-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNLKKRERA KTNASLISMV QRFSDITIMF AGLWLVCEVS GLSFLYMHLL
60 70 80 90 100
VALITLVVFQ MLGGITDFYR SWRGVRAATE FALLLQNWTL SVIFSAGLVA
110 120 130 140 150
FNNDFDTQLK IWLAWYALTS IGLVVCRSCI RIGAGWLRNH GYNKRMVAVA
160 170 180 190 200
GDLAAGQMLM ESFRNQPWLG FEVVGVYHDP KPGGVSNDWA GNLQQLVEDA
210 220 230 240 250
KAGKIHNVYI AMQMCDGARV KKLVHQLADT TCSVLLIPDV FTFNILHSRL
260 270 280 290 300
EEMNGVPVVP LYDTPLSGVN RLLKRAEDIV LATLILLLIS PVLCCIALAV
310 320 330 340 350
KLSSPGPVIF RQTRYGMDGK PIKVWKFRSM KVMENDKVVT QATQNDPRVT
360 370 380 390 400
KVGNFLRRTS LDELPQFINV LTGGMSIVGP RPHAVAHNEQ YRQLIEGYML
410 420 430 440 450
RHKVKPGITG WAQINGWRGE TDTLEKMEKR VEFDLEYIRE WSVWFDIKIV
460
FLTVFKGFVN KAAY
Length:464
Mass (Da):52,408
Last modified:November 1, 1997 - v2
Checksum:i0103B570EE51EBDB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti202 – 203AG → RA in AAC77848 (PubMed:8759852).Curated2
Sequence conflicti297A → P in AAC77848 (PubMed:8759852).Curated1
Sequence conflicti312Q → K in AAC77848 (PubMed:8759852).Curated1
Sequence conflicti399 – 400ML → IV in AAC77848 (PubMed:8759852).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U38473 Genomic DNA Translation: AAC77848.1
U00096 Genomic DNA Translation: AAC75108.1
AP009048 Genomic DNA Translation: BAA15900.1
PIRiF64970
RefSeqiNP_416551.1, NC_000913.3
WP_000183107.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75108; AAC75108; b2047
BAA15900; BAA15900; BAA15900
GeneIDi946583
KEGGiecj:JW2032
eco:b2047
PATRICifig|1411691.4.peg.204

Entry informationi

Entry nameiWCAJ_ECOLI
AccessioniPrimary (citable) accession number: P71241
Secondary accession number(s): P76381
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: April 25, 2018
This is version 126 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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