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Protein

UDP-glucose:undecaprenyl-phosphate glucose-1-phosphate transferase

Gene

wcaJ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Is the initiating enzyme for colanic acid (CA) synthesis. Catalyzes the transfer of the glucose-1-phosphate moiety from UDP-Glc onto the carrier lipid undecaprenyl phosphate (C55-P), forming a phosphoanhydride bond yielding to glucosyl-pyrophosphoryl-undecaprenol (Glc-PP-C55). Also possesses a weak galactose-1-P transferase activity.1 Publication

Catalytic activityi

UDP-glucose + ditrans,octacis-undecaprenyl phosphate = UMP + alpha-D-glucopyranosyl-diphospho-ditrans,octacis-undecaprenol.1 Publication

Pathwayi: colanic acid biosynthesis

This protein is involved in the pathway colanic acid biosynthesis, which is part of Exopolysaccharide biosynthesis.1 Publication
View all proteins of this organism that are known to be involved in the pathway colanic acid biosynthesis and in Exopolysaccharide biosynthesis.

GO - Molecular functioni

GO - Biological processi

  • colanic acid biosynthetic process Source: EcoCyc
  • lipopolysaccharide biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Exopolysaccharide synthesis, Lipopolysaccharide biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:G7098-MONOMER.
ECOL316407:JW2032-MONOMER.
UniPathwayiUPA00980.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-glucose:undecaprenyl-phosphate glucose-1-phosphate transferase (EC:2.7.8.31)
Short name:
UDP-Glc:Und-P Glc-1-P transferase
Alternative name(s):
Colanic acid biosynthesis UDP-glucose lipid carrier transferase
Glucosyl-P-P-undecaprenol synthase
Gene namesi
Name:wcaJ
Ordered Locus Names:b2047, JW2032
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13575. wcaJ.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1515CytoplasmicSequence analysisAdd
BLAST
Transmembranei16 – 3621HelicalSequence analysisAdd
BLAST
Topological domaini37 – 382PeriplasmicSequence analysis
Transmembranei39 – 5921HelicalSequence analysisAdd
BLAST
Topological domaini60 – 8021CytoplasmicSequence analysisAdd
BLAST
Transmembranei81 – 10121HelicalSequence analysisAdd
BLAST
Topological domaini102 – 1043PeriplasmicSequence analysis
Transmembranei105 – 12521HelicalSequence analysisAdd
BLAST
Topological domaini126 – 278153CytoplasmicSequence analysisAdd
BLAST
Transmembranei279 – 29921HelicalSequence analysisAdd
BLAST
Topological domaini300 – 464165PeriplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene show a non-mucoid phenotype.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 464464UDP-glucose:undecaprenyl-phosphate glucose-1-phosphate transferasePRO_0000166470Add
BLAST

Proteomic databases

PaxDbiP71241.
PRIDEiP71241.

Interactioni

Protein-protein interaction databases

BioGridi4263311. 180 interactions.
DIPiDIP-11125N.
IntActiP71241. 1 interaction.
STRINGi511145.b2047.

Structurei

3D structure databases

ProteinModelPortaliP71241.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the bacterial sugar transferase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105D3Q. Bacteria.
COG2148. LUCA.
HOGENOMiHOG000153662.
InParanoidiP71241.
KOiK03606.
OMAiREWSLWF.
OrthoDBiEOG6X10V2.
PhylomeDBiP71241.

Family and domain databases

InterProiIPR003362. Bact_transf.
IPR017475. EPS_sugar_tfrase.
IPR017473. Undecaprenyl-P_gluc_Ptfrase.
[Graphical view]
PfamiPF02397. Bac_transf. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03025. EPS_sugtrans. 1 hit.
TIGR03023. WcaJ_sugtrans. 1 hit.

Sequencei

Sequence statusi: Complete.

P71241-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNLKKRERA KTNASLISMV QRFSDITIMF AGLWLVCEVS GLSFLYMHLL
60 70 80 90 100
VALITLVVFQ MLGGITDFYR SWRGVRAATE FALLLQNWTL SVIFSAGLVA
110 120 130 140 150
FNNDFDTQLK IWLAWYALTS IGLVVCRSCI RIGAGWLRNH GYNKRMVAVA
160 170 180 190 200
GDLAAGQMLM ESFRNQPWLG FEVVGVYHDP KPGGVSNDWA GNLQQLVEDA
210 220 230 240 250
KAGKIHNVYI AMQMCDGARV KKLVHQLADT TCSVLLIPDV FTFNILHSRL
260 270 280 290 300
EEMNGVPVVP LYDTPLSGVN RLLKRAEDIV LATLILLLIS PVLCCIALAV
310 320 330 340 350
KLSSPGPVIF RQTRYGMDGK PIKVWKFRSM KVMENDKVVT QATQNDPRVT
360 370 380 390 400
KVGNFLRRTS LDELPQFINV LTGGMSIVGP RPHAVAHNEQ YRQLIEGYML
410 420 430 440 450
RHKVKPGITG WAQINGWRGE TDTLEKMEKR VEFDLEYIRE WSVWFDIKIV
460
FLTVFKGFVN KAAY
Length:464
Mass (Da):52,408
Last modified:November 1, 1997 - v2
Checksum:i0103B570EE51EBDB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti202 – 2032AG → RA in AAC77848 (PubMed:8759852).Curated
Sequence conflicti297 – 2971A → P in AAC77848 (PubMed:8759852).Curated
Sequence conflicti312 – 3121Q → K in AAC77848 (PubMed:8759852).Curated
Sequence conflicti399 – 4002ML → IV in AAC77848 (PubMed:8759852).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U38473 Genomic DNA. Translation: AAC77848.1.
U00096 Genomic DNA. Translation: AAC75108.1.
AP009048 Genomic DNA. Translation: BAA15900.1.
PIRiF64970.
RefSeqiNP_416551.1. NC_000913.3.
WP_000183107.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75108; AAC75108; b2047.
BAA15900; BAA15900; BAA15900.
GeneIDi946583.
KEGGiecj:JW2032.
eco:b2047.
PATRICi32119427. VBIEscCol129921_2124.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U38473 Genomic DNA. Translation: AAC77848.1.
U00096 Genomic DNA. Translation: AAC75108.1.
AP009048 Genomic DNA. Translation: BAA15900.1.
PIRiF64970.
RefSeqiNP_416551.1. NC_000913.3.
WP_000183107.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP71241.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263311. 180 interactions.
DIPiDIP-11125N.
IntActiP71241. 1 interaction.
STRINGi511145.b2047.

Proteomic databases

PaxDbiP71241.
PRIDEiP71241.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75108; AAC75108; b2047.
BAA15900; BAA15900; BAA15900.
GeneIDi946583.
KEGGiecj:JW2032.
eco:b2047.
PATRICi32119427. VBIEscCol129921_2124.

Organism-specific databases

EchoBASEiEB3345.
EcoGeneiEG13575. wcaJ.

Phylogenomic databases

eggNOGiENOG4105D3Q. Bacteria.
COG2148. LUCA.
HOGENOMiHOG000153662.
InParanoidiP71241.
KOiK03606.
OMAiREWSLWF.
OrthoDBiEOG6X10V2.
PhylomeDBiP71241.

Enzyme and pathway databases

UniPathwayiUPA00980.
BioCyciEcoCyc:G7098-MONOMER.
ECOL316407:JW2032-MONOMER.

Miscellaneous databases

PROiP71241.

Family and domain databases

InterProiIPR003362. Bact_transf.
IPR017475. EPS_sugar_tfrase.
IPR017473. Undecaprenyl-P_gluc_Ptfrase.
[Graphical view]
PfamiPF02397. Bac_transf. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03025. EPS_sugtrans. 1 hit.
TIGR03023. WcaJ_sugtrans. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Organization of the Escherichia coli K-12 gene cluster responsible for production of the extracellular polysaccharide colanic acid."
    Stevenson G., Andrianopoulos K., Hobbs M., Reeves P.R.
    J. Bacteriol. 178:4885-4893(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.
  6. "Functional characterization of UDP-glucose:undecaprenyl-phosphate glucose-1-phosphate transferases of Escherichia coli and Caulobacter crescentus."
    Patel K.B., Toh E., Fernandez X.B., Hanuszkiewicz A., Hardy G.G., Brun Y.V., Bernards M.A., Valvano M.A.
    J. Bacteriol. 194:2646-2657(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, ROLE IN COLANIC ACID BIOSYNTHESIS, PATHWAY, SUBSTRATE SPECIFICITY, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiWCAJ_ECOLI
AccessioniPrimary (citable) accession number: P71241
Secondary accession number(s): P76381
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: January 20, 2016
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Complements holdfast synthesis in the C.crescentus mutant lacking hfsE, pssY and pssZ genes. Can also partially complement the O-antigen defect in the S.typhimurium wbaP deletion strain (PubMed:22408159).1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.