Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Hydrogenase-4 transcriptional activator

Gene

hyfR

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Required for induction of expression of the hydrogenase-4 structural genes.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi375 – 3828ATPPROSITE-ProRule annotation
Nucleotide bindingi438 – 44710ATPPROSITE-ProRule annotation
DNA bindingi641 – 66020H-T-H motifBy similarityAdd
BLAST

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • DNA binding Source: EcoliWiki
  • sequence-specific DNA binding Source: InterPro

GO - Biological processi

  • DNA-templated transcription, initiation Source: EcoliWiki
  • phosphorelay signal transduction system Source: UniProtKB-KW
  • regulation of DNA-templated transcription, initiation Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation, Two-component regulatory system

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:G7308-MONOMER.
ECOL316407:JW2476-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydrogenase-4 transcriptional activator
Gene namesi
Name:hyfR
Ordered Locus Names:b2491, JW2476
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14219. hyfR.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 670670Hydrogenase-4 transcriptional activatorPRO_0000081299Add
BLAST

Proteomic databases

EPDiP71229.
PaxDbiP71229.
PRIDEiP71229.

Interactioni

Protein-protein interaction databases

BioGridi4261431. 6 interactions.
DIPiDIP-9993N.
IntActiP71229. 13 interactions.
MINTiMINT-1219276.
STRINGi511145.b2491.

Structurei

3D structure databases

ProteinModelPortaliP71229.
SMRiP71229. Positions 305-661.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini169 – 311143GAFAdd
BLAST
Domaini347 – 576230Sigma-54 factor interactionPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 GAF domain.Curated
Contains 1 sigma-54 factor interaction domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4108JUA. Bacteria.
COG3604. LUCA.
HOGENOMiHOG000058488.
InParanoidiP71229.
KOiK12146.
OMAiSWLNDEA.
OrthoDBiEOG6WHNMG.
PhylomeDBiP71229.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
3.30.450.40. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003018. GAF.
IPR029016. GAF_dom-like.
IPR009057. Homeodomain-like.
IPR002197. HTH_Fis.
IPR027417. P-loop_NTPase.
IPR002078. Sigma_54_int.
IPR025662. Sigma_54_int_dom_ATP-bd_1.
IPR025943. Sigma_54_int_dom_ATP-bd_2.
IPR025944. Sigma_54_int_dom_CS.
[Graphical view]
PfamiPF13185. GAF_2. 1 hit.
PF02954. HTH_8. 1 hit.
PF00158. Sigma54_activat. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00065. GAF. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF55781. SSF55781. 1 hit.
PROSITEiPS00675. SIGMA54_INTERACT_1. 1 hit.
PS00676. SIGMA54_INTERACT_2. 1 hit.
PS00688. SIGMA54_INTERACT_3. 1 hit.
PS50045. SIGMA54_INTERACT_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P71229-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAMSDEAMFA PPQGITIEAV NGMLAERLAQ KHGKASLLRA FIPLPPPFSP
60 70 80 90 100
VQLIELHVLK SNFYYRYHDD GSDVTATTEY QGEMVDYSRH AVLLGSSGMA
110 120 130 140 150
ELRFIRTHGS RFTSQDCTLF NWLARIITPV LQSWLNDEEQ QVALRLLEKD
160 170 180 190 200
RDHHRVLVDI TNAVLSHLDL DDLIADVARE IHHFFGLASV SMVLGDHRKN
210 220 230 240 250
EKFSLWCSDL SASHCACLPR CMPGESVLLT QTLQTRQPTL THRADDLFLW
260 270 280 290 300
QRDPLLLLLA SNGCESALLI PLTFGNHTPG ALLLAHTSST LFSEENCQLL
310 320 330 340 350
QHIADRIAIA VGNADAWRSM TDLQESLQQE NHQLSEQLLS NLGIGDIIYQ
360 370 380 390 400
SQAMEDLLQQ VDIVAKSDST VLICGETGTG KEVIARAIHQ LSPRRDKPLV
410 420 430 440 450
KINCAAIPAS LLESELFGHD KGAFTGAINT HRGRFEIADG GTLFLDEIGD
460 470 480 490 500
LPLELQPKLL RVLQEREIER LGGSRTIPVN VRVIAATNRD LWQMVEDRQF
510 520 530 540 550
RSDLFYRLNV FPLELPPLRD RPEDIPLLAK HFTQKMARHM NRAIDAIPTE
560 570 580 590 600
ALRQLMSWDW PGNVRELENV IERAVLLTRG NSLNLHLNVR QSRLLPTLNE
610 620 630 640 650
DSALRSSMAQ LLHPTTPEND EEERQRIVQV LRETNGIVAG PRGAATRLGM
660 670
KRTTLLSRMQ RLGISVREVL
Length:670
Mass (Da):75,305
Last modified:February 1, 1997 - v1
Checksum:i0EA738D2D6BA96B6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63654 Genomic DNA. Translation: AAB88573.1.
U00096 Genomic DNA. Translation: AAC75544.2.
AP009048 Genomic DNA. Translation: BAA16380.1.
PIRiB65025.
RefSeqiNP_416986.4. NC_000913.3.
WP_001251544.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75544; AAC75544; b2491.
BAA16380; BAA16380; BAA16380.
GeneIDi948886.
KEGGiecj:JW2476.
eco:b2491.
PATRICi32120369. VBIEscCol129921_2587.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63654 Genomic DNA. Translation: AAB88573.1.
U00096 Genomic DNA. Translation: AAC75544.2.
AP009048 Genomic DNA. Translation: BAA16380.1.
PIRiB65025.
RefSeqiNP_416986.4. NC_000913.3.
WP_001251544.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP71229.
SMRiP71229. Positions 305-661.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261431. 6 interactions.
DIPiDIP-9993N.
IntActiP71229. 13 interactions.
MINTiMINT-1219276.
STRINGi511145.b2491.

Proteomic databases

EPDiP71229.
PaxDbiP71229.
PRIDEiP71229.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75544; AAC75544; b2491.
BAA16380; BAA16380; BAA16380.
GeneIDi948886.
KEGGiecj:JW2476.
eco:b2491.
PATRICi32120369. VBIEscCol129921_2587.

Organism-specific databases

EchoBASEiEB3971.
EcoGeneiEG14219. hyfR.

Phylogenomic databases

eggNOGiENOG4108JUA. Bacteria.
COG3604. LUCA.
HOGENOMiHOG000058488.
InParanoidiP71229.
KOiK12146.
OMAiSWLNDEA.
OrthoDBiEOG6WHNMG.
PhylomeDBiP71229.

Enzyme and pathway databases

BioCyciEcoCyc:G7308-MONOMER.
ECOL316407:JW2476-MONOMER.

Miscellaneous databases

PROiP71229.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
3.30.450.40. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003018. GAF.
IPR029016. GAF_dom-like.
IPR009057. Homeodomain-like.
IPR002197. HTH_Fis.
IPR027417. P-loop_NTPase.
IPR002078. Sigma_54_int.
IPR025662. Sigma_54_int_dom_ATP-bd_1.
IPR025943. Sigma_54_int_dom_ATP-bd_2.
IPR025944. Sigma_54_int_dom_CS.
[Graphical view]
PfamiPF13185. GAF_2. 1 hit.
PF02954. HTH_8. 1 hit.
PF00158. Sigma54_activat. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00065. GAF. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF55781. SSF55781. 1 hit.
PROSITEiPS00675. SIGMA54_INTERACT_1. 1 hit.
PS00676. SIGMA54_INTERACT_2. 1 hit.
PS00688. SIGMA54_INTERACT_3. 1 hit.
PS50045. SIGMA54_INTERACT_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Andrews S.C., McClay J., Ambler A., Quail M., Berks B.C., Guest J.R.
    Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
    Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
    , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
    DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiHYFR_ECOLI
AccessioniPrimary (citable) accession number: P71229
Secondary accession number(s): P76567, P76976, P76977
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: May 11, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.