P71140 (P71140_CLOTM) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Endoglucanase J EMBL BAA12070.1

Gene names

Name:

celJ EMBL BAA12070.1

Organism

Clostridium thermocellum EMBL BAA12070.1

Taxonomic identifier

1515 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	1601 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Ligand	Calcium PDB 2E0P PDB 2E4T PDB 2EEX PDB 2EJ1 PDB 2EO7 PDB 2EQD PDB 2C4X Metal-binding PDB 2E0P PDB 2E4T PDB 2EEX PDB 2EJ1 PDB 2EO7 PDB 2EQD PDB 2C4X Zinc PDB 2E0P PDB 2E4T PDB 2EEX PDB 2EJ1 PDB 2EO7 PDB 2EQD
Technical term	3D-structure PDB 2E0P PDB 2E4T PDB 2EEX PDB 2EJ1 PDB 2EO7 PDB 2EQD PDB 2C4X PDB 1WMX PDB 2C24
Gene Ontology (GO)
Biological process	polysaccharide catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	cellulase activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Metal binding	803	1	Zinc PDB 2E0P PDB 2E4T PDB 2EEX PDB 2EJ1 PDB 2EO7 PDB 2EQD
	Metal binding	822	1	Calcium 1 PDB 2E0P PDB 2E4T PDB 2EEX PDB 2EJ1 PDB 2EO7 PDB 2EQD
	Metal binding	918	1	Calcium 1; via carbonyl oxygen PDB 2E0P PDB 2E4T PDB 2EEX PDB 2EJ1 PDB 2EO7 PDB 2EQD
	Metal binding	921	1	Calcium 1 PDB 2E0P PDB 2E4T PDB 2EEX PDB 2EJ1 PDB 2EO7 PDB 2EQD
	Metal binding	923	1	Calcium 1; via carbonyl oxygen PDB 2E0P PDB 2E4T PDB 2EEX PDB 2EJ1 PDB 2EO7 PDB 2EQD
	Metal binding	1163	1	Zinc PDB 2E0P PDB 2E4T PDB 2EEX PDB 2EJ1 PDB 2EO7 PDB 2EQD
	Metal binding	1169	1	Zinc PDB 2E0P PDB 2E4T PDB 2EEX PDB 2EJ1 PDB 2EO7 PDB 2EQD
	Metal binding	1356	1	Calcium 2 PDB 2C4X
	Metal binding	1357	1	Calcium 2; via carbonyl oxygen PDB 2C4X
	Metal binding	1385	1	Calcium 2 PDB 2C4X
	Metal binding	1387	1	Calcium 2 PDB 2C4X
	Metal binding	1428	1	Calcium 2 PDB 2C4X
	Metal binding	1448	1	Calcium 3 PDB 2C4X
	Metal binding	1453	1	Calcium 3; via carbonyl oxygen PDB 2C4X
	Metal binding	1455	1	Calcium 3 PDB 2C4X
	Metal binding	1482	1	Calcium 3; via carbonyl oxygen PDB 2C4X
	Metal binding	1485	1	Calcium 3; via carbonyl oxygen PDB 2C4X
	Metal binding	1597	1	Calcium 3 PDB 2C4X

Sequences

	Sequence		Length	Mass (Da)
	P71140 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 31E85D77F8642565	FASTA	1,601	178,059
	10 20 30 40 50 60 MAKRRLSLLL VLAIMFTMVV PQISASAETV APEGYRKLLD VQIFKDSPVV GWSGSGMGEL 70 80 90 100 110 120 ETIGDTLPVD TTVTYNGLPT LRLNVQTTVQ SGWWISLLTL RGWNTHDLSQ YVENGYLEFD 130 140 150 160 170 180 IKGKEGGEDF VIGFRDKVYE RVYGLEIDVT TVISNYVTVT TDWQHVKIPL RDLMKINNGF 190 200 210 220 230 240 DPSSVTCLVF SKRYADPFTV WFSDIKITSE DNEKSAPAIK VNQLGFIPEA EKYALVTGFA 250 260 270 280 290 300 EELAVSEGDE FAVINAADNS VAYTGKLTLV TEYEPLDSGE KILKADFSDL TVPGKYYISI 310 320 330 340 350 360 EGLDNSPKFE IGEGIYGPLV VDAARYFYYQ RQGIELEEPY AQGYPRKDVT PQDAYAVFAS 370 380 390 400 410 420 GKKDPIDITK GWYDAGDFGK YVNAGATGVS DLFWAYEMFP SQFVDGQFNI PESGNGVPDI 430 440 450 460 470 480 LDEARWELEW MLKMQDKESG GFYPRVQSDN DENIKSRIIR DQNGCTTDDT ACAAGILAHA 490 500 510 520 530 540 YLIYKDIDPD FAQECLDAAI NAWKFLEKNP ENIVSPPGPY NVYDDSGDRL WAAASLYRAT 550 560 570 580 590 600 GEEVYHTYFK QNYKSFAQKF ESPTAYAHTW GDMWLTAFLS YLKAENKDQE VVDWIDTEFG 610 620 630 640 650 660 IWLENILTRY ENNPWKNAIV PGNYFWGINM QVMNVPMDAI IGSQLLGKYS DRIEKLGFGS 670 680 690 700 710 720 LNWLLGTNPL RFSFVSGYGE DSVKGVFSNI YNTDGKQGIP KGYMPGGPNA YEGAGLSRFA 730 740 750 760 770 780 AKCYTRSTGD WVANEHTVYW NSALVFMAAF ANQGSEVNPG PAPEPGVTPN PTEPAKVVDI 790 800 810 820 830 840 RIDTSAERKP ISPYIYGSNQ ELDATVTAKR FGGNRTTGYN WENNFSNAGS DWLHYSDTYL 850 860 870 880 890 900 LEDGGVPKGE WSTPASVVTT FHDKALSKNV PYTLITLQAA GYVSADGNGP VSQEETAPSS 910 920 930 940 950 960 RWKEVKFEKG APFSLTPDTE DDYVYMDEFV NYLVNKYGNA STPTGIKGYS IDNEPALWSH 970 980 990 1000 1010 1020 THPRIHPDNV TAKELIEKSV ALSKAVKKVD PYAEIFGPAL YGFAAYETLQ SAPDWGTEGE 1030 1040 1050 1060 1070 1080 GYRWFIDYYL DKMKKASDEE GKRLLDVLDV HWYPEARGGG ERICFGADPR NIETNKARLQ 1090 1100 1110 1120 1130 1140 APRTLWDPTY IEDSWIGQWK KDFLPILPNL LDSIEKYYPG TKLAITEYDY GGGNHITGGI 1150 1160 1170 1180 1190 1200 AQADVLGIFG KYGVYLATFW GDASNNYTEA GINLYTNYDG KGGKFGDTSV KCETSDIEVS 1210 1220 1230 1240 1250 1260 SAYASIVGED DSKLHIILLN KNYDQPTTFN FSIDSSKNYT IGNVWAFDRG SSNITQRTPI 1270 1280 1290 1300 1310 1320 VNIKDNTFTY TVPALTACHI VLEAAEPVVY GDLNNDSKVN AVDIMMLKRY ILGIIDNINL 1330 1340 1350 1360 1370 1380 TAADIYFDGV VNSSDYNIMK RYLLKAIEDI PYVPENQAPK AIFTFSPEDP VTDENVVFNA 1390 1400 1410 1420 1430 1440 SNSIDEDGTI AYYAWDFGDG YEGTSTTPTI TYKYKNPGTY KVKLIVTDNQ GASSSFTATI 1450 1460 1470 1480 1490 1500 KVTSATGDNS KFNFEDGTLG GFTTSGTNAT GVVVNTTEKA FKGERGLKWT VTSEGEGTAE 1510 1520 1530 1540 1550 1560 LKLDGGTIVV PGTTMTFRIW IPSGAPIAAI QPYIMPHTPD WSEVLWNSTW KGYTMVKTDD 1570 1580 1590 1600 WNEITLTLPE DVDPTWPQQM GIQVQTIDEG EFTIYVDAID W

References

[1]	"Cloning, DNA sequencing, and expression of the gene encoding Clostridium thermocellum cellulase CelJ, the largest catalytic component of the cellulosome." Ahsan M.M., Kimura T., Karita S., Sakka K., Ohmiya K. J. Bacteriol. 178:5732-5740(1996) [PubMed: 8824619] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: F1 EMBL BAA12070.1.
[2]	"Crystal Structure of Family 30 Carbohydrate Binding Module." Horiguchi Y., Kono M., Suzuki A., Yamane T., Arai M., Sakka K., Omiya K. Submitted (JUL-2004) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 31-235.
[3]	"Crystal structure of Cel44A, a glycoside hydrolase family 44 endoglucanase from Clostridium thermocellum." Kitago Y., Karita S., Watanabe N., Kamiya M., Aizawa T., Sakka K., Tanaka I. J. Biol. Chem. 282:35703-35711(2007) [PubMed: 17905739] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (0.96 ANGSTROMS) OF 773-1287 IN COMPLEX WITH CALCIUM AND ZINC.
[4]	"Xyloglucan is recognized by carbohydrate-binding modules that interact with beta-glucan chains." Najmudin S., Guerreiro C.I., Carvalho A.L., Prates J.A., Correia M.A., Alves V.D., Ferreira L.M., Romao M.J., Gilbert H.J., Bolam D.N., Fontes C.M. J. Biol. Chem. 281:8815-8828(2006) [PubMed: 16314409] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 1353-1601 IN COMPLEX WITH CALCIUM.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D83704 Genomic DNA. Translation: BAA12070.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1WMX	X-ray	2.00	A/B	31-235	[»]
2C24	X-ray	2.27	A/B	24-220	[»]
2C4X	X-ray	2.00	A	1353-1601	[»]
2E0P	X-ray	1.60	A	773-1287	[»]
2E4T	X-ray	0.96	A	773-1287	[»]
2EEX	X-ray	2.00	A	773-1287	[»]
2EJ1	X-ray	1.80	A	773-1287	[»]
2EO7	X-ray	1.75	A	773-1287	[»]
2EQD	X-ray	2.80	A	773-1287	[»]

ProteinModelPortal

P71140.

SMR

P71140. Positions 34-228, 775-1283, 1354-1601.

ModBase

Search...

Protein family/group databases

CAZy

CBM30. Carbohydrate-Binding Module Family 30.
CBM44. Carbohydrate-Binding Module Family 44.
GH44. Glycoside Hydrolase Family 44.
GH9. Glycoside Hydrolase Family 9.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR016134. Cellulos_enz_dockerin_1.
IPR018242. Dockerin_1.
IPR008979. Galactose-bd-like.
IPR001701. Glyco_hydro_9.
IPR004197. Glyco_hydro_9_Ig-like.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR022409. PKD/Chitinase_dom.
IPR000601. PKD_dom.
[Graphical view]

Gene3D

G3DSA:1.50.10.10. CelA/Cel48F_cat. 1 hit.
G3DSA:1.10.1330.10. Cellulos_enz_dockerin_1. 1 hit.
G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
G3DSA:2.60.40.670. PKD. 1 hit.

Pfam

PF02927. CelD_N. 1 hit.
PF00404. Dockerin_1. 2 hits.
PF00759. Glyco_hydro_9. 1 hit.
PF00801. PKD. 1 hit.
[Graphical view]

SMART

SM00089. PKD. 1 hit.
[Graphical view]

SUPFAM

SSF63446. Cellulos_enz_dockerin_1. 1 hit.
SSF49785. Gal_bind_like. 2 hits.
SSF51445. Glyco_hydro_cat. 1 hit.
SSF48208. Glyco_trans_6hp. 1 hit.
SSF81296. Ig_E-set. 1 hit.
SSF49299. PKD. 1 hit.

PROSITE

PS50093. PKD. 1 hit.
[Graphical view]

ProtoNet

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Entry information

Entry name

P71140_CLOTM

Accession

Primary (citable) accession number: P71140

Entry history

Integrated into UniProtKB/TrEMBL:	February 1, 1997
Last sequence update:	February 1, 1997
Last modified:	September 21, 2011
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)