Reviewed, UniProtKB/Swiss-Prot P71128 (CYSM_CAMJE)

Names and origin

Protein names

Recommended name:
    Cysteine synthase B
      Short name=CSase B
    EC=2.5.1.47
Alternative name(s):
    O-acetylserine sulfhydrylase B
    O-acetylserine (thiol)-lyase B
      Short name=OAS-TL B

Gene names

Name:	cysM
Ordered Locus Names:	Cj0912c

Organism

Campylobacter jejuni [Complete proteome] [HAMAP]

Taxonomic identifier

197 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	299 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	O(3)-acetyl-L-serine + H2S = L-cysteine + acetate.
Cofactor	Pyridoxal phosphate.
Pathway	Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 2/2.
Sequence similarities	Belongs to the cysteine synthase/cystathionine beta-synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process from serine Inferred from electronic annotation. Source: InterPro
Molecular function	cysteine synthase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 299	299	Cysteine synthase B		PRO_0000167107
Regions
	Region	174 – 178	5	Pyridoxal phosphate binding By similarity
Sites
	Binding site	70	1	Pyridoxal phosphate By similarity
	Binding site	261	1	Pyridoxal phosphate By similarity
Amino acid modifications
	Modified residue	40	1	N6-(pyridoxal phosphate)lysine By similarity
Experimental info
	Sequence conflict	87	1	I → V in AAC44853. Ref.1
	Sequence conflict	99	1	R → K in AAC44853. Ref.1
	Sequence conflict	235	1	E → Q in AAC44853. Ref.1
	Sequence conflict	254	1	S → I in AAC44853. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P71128-1 [UniParc]. Last modified December 1, 2000. Version 2. Checksum: 8298782DA1852ACB	FASTA	299	32,385
	10 20 30 40 50 60 MKVHEKVSEL IGNTPIIHLK KFGINVFAKC EFLNPSHSIK DRAAFEMIKD ALDSKKINQD 70 80 90 100 110 120 TTIVEATSGN TGISLAMICA DLGLKFIAVM PESMSLERRK MITLFGARLE LTPANLGMKG 130 140 150 160 170 180 AVDKANEILL NTPNSFMVSQ FENISNKNAH RKNTALEILR DLDNELDIFV AGFGTGGTIS 190 200 210 220 230 240 GVGEILKEKL EKVHIVGVEP LNSPLLSKGE AGSHKIQGIG ANFIPAILNK EVIDEVITVS 250 260 270 280 290 NEDAINTAKE LAKSGLMVGI SSGANVFAAS MLAKKFPDKR ILTMLNDTAE RYLSTDLFA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a functional homolog of the Escherichia coli and Salmonella typhimurium cysM gene encoding O-acetylserine sulfhydrylase B in Campylobacter jejuni." Garvis S.G., Tipton S.L., Konkel M.E. Gene 185:63-67(1997) [PubMed: 9034314] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: F38011.
[2]	"The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences." Parkhill J., Wren B.W., Mungall K.L., Ketley J.M., Churcher C.M., Basham D., Chillingworth T., Davies R.M., Feltwell T., Holroyd S., Jagels K., Karlyshev A.V., Moule S., Pallen M.J., Penn C.W., Quail M.A., Rajandream M.A., Rutherford K.M. , van Vliet A.H.M., Whitehead S., Barrell B.G. Nature 403:665-668(2000) [PubMed: 10688204] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 11168 / Serotype O:2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U63157 Genomic DNA. Translation: AAC44853.1. AL111168 Genomic DNA. Translation: CAL35032.1.
PIR	G81364. JC6185.
RefSeq	YP_002344310.1.
3D structure databases
SMR	P71128. Positions 4-295.
ModBase	Search...
Genome annotation databases
GeneID	906009.
GenomeReviews	Gene locus Cj0912c in contig AL111168_GR.
KEGG	cje:Cj0912c.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG748215.
OMA	GINVFAK.
Enzyme and pathway databases
BioCyc	CJEJ192222:CJ0912C-MONOMER.
BRENDA	2.5.1.47. 255835.
Family and domain databases
InterPro	IPR001216. Cys_synth_BS. IPR005856. Cys_synthKM. IPR005859. CysK. IPR001926. PyrdxlP-dep_enz_bsu. IPR000634. Ser/Thr_deHydtase_PyrdxlP-BS. [Graphical view]
Pfam	PF00291. PALP. 1 hit. [Graphical view]
TIGRFAMs	TIGR01139. cysK. 1 hit. TIGR01136. cysKM. 1 hit.
PROSITE	PS00901. CYS_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSM_CAMJE

Accession

Primary (citable) accession number: P71128
Secondary accession number(s): Q0P9Y7, Q9PP20

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	December 1, 2000
Last modified:	February 9, 2010
This is version 73 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)