P71104 (ACKA_CLOAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetate kinase
EC=2.7.2.1

Alternative name(s):
Acetokinase

Gene names

Name:	ackA
Synonyms:	ack
Ordered Locus Names:	CA_C1743

Organism

Clostridium acetobutylicum

Taxonomic identifier

1488 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	401 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the activation of acetate to acetyl CoA and the secretion of acetate By similarity. HAMAP MF_00020
Catalytic activity	ATP + acetate = ADP + acetyl phosphate. HAMAP MF_00020 ATP + propanoate = ADP + propanoyl phosphate. HAMAP MF_00020
Pathway	Metabolic intermediate biosynthesis; acetyl-CoA biosynthesis; acetyl-CoA from acetate: step 1/2. HAMAP MF_00020
Subcellular location	Cytoplasm By similarity HAMAP MF_00020.
Sequence similarities	Belongs to the acetokinase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	organic acid metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 401	401	Acetate kinase HAMAP MF_00020		PRO_0000107545
Experimental info
	Sequence conflict	61	1	K → R in AAB18301. Ref.1
	Sequence conflict	232	1	L → P in AAB18301. Ref.1
	Sequence conflict	239	1	T → A in AAB18301. Ref.1
	Sequence conflict	306	1	N → T in AAB18301. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P71104 [UniParc]. Last modified August 29, 2001. Version 2. Checksum: D005072C030B8774	FASTA	401	44,342
	10 20 30 40 50 60 MKNLVINCGS SSIKYQFIDM KDETVLAKGL VERIGIKGSV ITHKVNGEKY VTETPMEDHK 70 80 90 100 110 120 KAIKLVLDAL LNDEYGVIKN IDEISAVGHR IVHGGEKYAN SVLIDEDVMK SIEDCVSLAP 130 140 150 160 170 180 LHNPPHIIGI NACKELMPNV PMVAVFDTAF HQTIPDYAYM YAIPYEYYDK YKIRKYGFHG 190 200 210 220 230 240 TSHKYVSRTA AEFIGKKVED LKMVVCHMGN GASITAVENG KSVDTSMGFT PLGGLAMGTR 250 260 270 280 290 300 SGDMDPAVVT FLMDKLNINA SEVNNLLNKK SGIEGLSGIS SDMRDIKKGN YVDKDPKAML 310 320 330 340 350 360 AYSVFNYKIK QFIGSYTAVM NGLDCLVFTG GIGENSFENR REICKNMDYL GIKIDDKKND 370 380 390 400 ETMGIPMDIS AEGSKVRVLV IPTNEELMIA RDTKDIVGKL K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, sequencing, and expression of genes encoding phosphotransacetylase and acetate kinase from Clostridium acetobutylicum ATCC 824." Boynton Z.L., Bennett G.N., Rudolph F.B. Appl. Environ. Microbiol. 62:2758-2766(1996) [PubMed: 8702268] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
[2]	"Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum." Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V., Smith D.R. J. Bacteriol. 183:4823-4838(2001) [PubMed: 11466286] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U38234 Genomic DNA. Translation: AAB18301.1. AE001437 Genomic DNA. Translation: AAK79709.1.
PIR	B97115.
RefSeq	NP_348369.1. NC_003030.1.
3D structure databases
ProteinModelPortal	P71104.
SMR	P71104. Positions 1-397.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1117926.
GenomeReviews	Gene locus CA_C1743 in contig AE001437_GR.
KEGG	cac:CA_C1743.
NMPDR	fig|272562.1.peg.1898.
PATRIC	32037864. VBICloAce74127_1945.
Phylogenomic databases
HOGENOM	HBG285863.
OMA	HQTIPDY.
PhylomeDB	P71104.
Enzyme and pathway databases
BioCyc	CACE272562:CAC1743-MONOMER. MetaCyc:ACKCLOS-MONOMER.
Family and domain databases
HAMAP	MF_00020. Acetate_kinase. [Tree]
InterPro	IPR004372. Ac/Proprionate_kinase. IPR000890. Aliphatic_acid_kin_short-chain. IPR023865. Aliphatic_acid_kinase_CS. [Graphical view]
KO	K00925.
PANTHER	PTHR21060. Acetate_kin. 1 hit.
Pfam	PF00871. Acetate_kinase. 1 hit. [Graphical view]
PIRSF	PIRSF000722. Acetate_prop_kin. 1 hit.
PRINTS	PR00471. ACETATEKNASE.
TIGRFAMs	TIGR00016. AckA. 1 hit.
PROSITE	PS01075. ACETATE_KINASE_1. 1 hit. PS01076. ACETATE_KINASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACKA_CLOAB

Accession

Primary (citable) accession number: P71104

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 29, 2001
Last modified:	January 25, 2012
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program