Reviewed, UniProtKB/Swiss-Prot P71079 (FABL_BACSU)

Names and origin

Protein names

Recommended name:
    Enoyl-[acyl-carrier-protein] reductase [NADPH]
    EC=1.3.1.10
Alternative name(s):
    Enoyl-acyl carrier protein reductase III
    NADPH-dependent enoyl-ACP reductase

Gene names

Name:	fabL
Synonyms:	yfhR, ygaA
Ordered Locus Names:	BSU08650

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	250 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Acyl-[acyl-carrier-protein] + NADP+ = trans-2,3-dehydroacyl-[acyl-carrier-protein] + NADPH. Ref.5
Enzyme regulation	Inhibited by triclosan. Ref.5
Induction	Expressed during exponential growth. Ref.4
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.
Biophysicochemical properties	Kinetic parameters: KM=16 µM for NADPH

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 250	250	Enoyl-[acyl-carrier-protein] reductase [NADPH]		PRO_0000377006
Regions
	Nucleotide binding	10 – 17	8	NADP By similarity
Sites
	Active site	151	1	Proton acceptor By similarity
	Binding site	141	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P71079-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 4314962C70E19C49	FASTA	250	27,178
	10 20 30 40 50 60 MEQNKCALVT GSSRGVGKAA AIRLAENGYN IVINYARSKK AALETAEEIE KLGVKVLVVK 70 80 90 100 110 120 ANVGQPAKIK EMFQQIDETF GRLDVFVNNA ASGVLRPVME LEETHWDWTM NINAKALLFC 130 140 150 160 170 180 AQEAAKLMEK NGGGHIVSIS SLGSIRYLEN YTTVGVSKAA LEALTRYLAV ELSPKQIIVN 190 200 210 220 230 240 AVSGGAIDTD ALKHFPNRED LLEDARQNTP AGRMVEIKDM VDTVEFLVSS KADMIRGQTI 250 IVDGGRSLLV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequencing of a 27.8-kb nucleotide sequence of the 79 degrees-81 degrees region of the Bacillus subtilis genome containing the sspE locus." Yamamoto H., Uchiyama S., Sekiguchi J. DNA Res. 3:257-262(1996) [PubMed: 8946165] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The Bacillus subtilis 168 chromosome from sspE to katA." Cummings N.J., Connerton I.F. Microbiology 143:1855-1859(1997) [PubMed: 9202460] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[3]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[4]	"Transcription of genes near the sspE locus of the Bacillus subtilis genome." Yamamoto H., Mori M., Sekiguchi J. Microbiology 145:2171-2180(1999) [PubMed: 10463184] [Abstract] Cited for: INDUCTION. Strain: 168.
[5]	"The enoyl-[acyl-carrier-protein] reductases FabI and FabL from Bacillus subtilis." Heath R.J., Su N., Murphy C.K., Rock C.O. J. Biol. Chem. 275:40128-40133(2000) [PubMed: 11007778] [Abstract] Cited for: CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION.
[6]	"Crystallization and preliminary X-ray crystallographic analysis of enoyl-ACP reductase III (FabL) from Bacillus subtilis." Kim K.-H., Park J.K., Ha B.H., Moon J.H., Kim E.E. Acta Crystallogr. F 63:246-248(2007) [PubMed: 17329825] [Abstract] Cited for: CRYSTALLIZATION. Strain: 168.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D85082 Genomic DNA. Translation: BAA24484.1. Z82044 Genomic DNA. Translation: CAB04808.1. AL009126 Genomic DNA. Translation: CAB12693.1.
PIR	B69802.
RefSeq	NP_388745.1.
3D structure databases
HSSP	HSSP built from PDB template 1E7W based on UniProtKB Q01782.
ModBase	Search...
Genome annotation databases
GeneID	939223.
GenomeReviews	Gene locus BSU08650 in contig AL009126_GR.
KEGG	bsu:BSU08650.
NMPDR	fig|224308.1.peg.864.
Organism-specific databases
SubtiList	BG12221. fabL. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	P71079.
OMA	RPLMELE.
Enzyme and pathway databases
BioCyc	BSUB224308:BSU0864-MON.
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
PROSITE	PS00061. ADH_SHORT. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FABL_BACSU

Accession

Primary (citable) accession number: P71079
Secondary accession number(s): Q796Z2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 16, 2009
Last sequence update:	February 1, 1997
Last modified:	November 3, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)