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Protein
Submitted name:

Alpha-amylase

Gene
N/A
Organism
Bacillus sp. MK 716
Status
Unreviewed-Annotation score: Annotation score: 1 out of 5-Protein predictedi

Functioni

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi139Calcium 1UniRule annotation1
Metal bindingi196Calcium 2UniRule annotation1
Metal bindingi218Calcium 2; via carbonyl oxygenUniRule annotation1
Metal bindingi220Calcium 2UniRule annotation1
Metal bindingi231Calcium 1UniRule annotation1
Metal bindingi237Calcium 1UniRule annotation1
Metal bindingi239Calcium 2UniRule annotation1
Active sitei268NucleophileUniRule annotation1
Metal bindingi272Calcium 1; via carbonyl oxygenUniRule annotation1
Active sitei298Proton donorUniRule annotation1
Metal bindingi337Calcium 3; via carbonyl oxygenUniRule annotation1
Metal bindingi441Calcium 3UniRule annotation1
Metal bindingi464Calcium 3UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

LigandCalciumUniRule annotation, Metal-bindingUniRule annotation

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Submitted name:
Alpha-amylaseImported
OrganismiBacillus sp. MK 716Imported
Taxonomic identifieri54116 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 34Sequence analysisAdd BLAST34
ChainiPRO_500416123835 – 521Sequence analysisAdd BLAST487

Structurei

3D structure databases

ProteinModelPortaliP71034.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini40 – 446AamyInterPro annotationAdd BLAST407

Keywords - Domaini

SignalSequence analysis

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiView protein in InterPro
IPR013776. A-amylase_thermo.
IPR015237. Alpha-amylase_C_pro.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
PfamiView protein in Pfam
PF00128. Alpha-amylase. 1 hit.
PF09154. DUF1939. 1 hit.
PIRSFiPIRSF001021. Alph-amls_thrmst. 1 hit.
SMARTiView protein in SMART
SM00642. Aamy. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

P71034-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLTFHRIIRK GWMFLLAFLL TASLFCPTGQ PAKAAAPFNG TMMQYFEWYL
60 70 80 90 100
PDDGTLWTKV ANEANNLSSL GITALWLPPA YKGTSRSDVG YGVYDLYDLG
110 120 130 140 150
EFNQKGAVRT KYGTKAQYLQ AIQAAHAAGM QVYADVVFDH KGGADGTEWV
160 170 180 190 200
DAVEVNPSDR NQEISGTYQI QAWTKFDFPG RGNTYSSFKW RWYHFDGVDW
210 220 230 240 250
DESRKLSRIY KFRGIGKAWD WEVDTENGNY DYLMYADLDM DHPEVVTELK
260 270 280 290 300
NWGKWYVNTT NIDGFRLDAV KHIKFSFFPD WLSYVRSQTG KPLFTVGEYW
310 320 330 340 350
SYDINKLHNY ITKTNGTMSL FDAPLHNKFY TASKSGGAFD MRTLMTNTLM
360 370 380 390 400
KDQPTLAVTF VDNHDTEPGQ ALQSWVDPWF KPLAYAFILT RQEGYPCVFY
410 420 430 440 450
GDYYGIPQYN IPSLKSKIDP LLIARRDYAY GTQHDYLDHS DIIGWTREGV
460 470 480 490 500
TEKPGSGLAA LITDGPGGSK WMYVGKQHAG KVFYDLTGNR SDTVTINSDG
510 520
WGEFKVNGGS VSVWVPRRPV N
Length:521
Mass (Da):59,312
Last modified:February 1, 1997 - v1
Checksum:i5612A88596D922E1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U75445 Genomic DNA. Translation: AAB18785.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U75445 Genomic DNA. Translation: AAB18785.1.

3D structure databases

ProteinModelPortaliP71034.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiView protein in InterPro
IPR013776. A-amylase_thermo.
IPR015237. Alpha-amylase_C_pro.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
PfamiView protein in Pfam
PF00128. Alpha-amylase. 1 hit.
PF09154. DUF1939. 1 hit.
PIRSFiPIRSF001021. Alph-amls_thrmst. 1 hit.
SMARTiView protein in SMART
SM00642. Aamy. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiP71034_9BACI
AccessioniPrimary (citable) accession number: P71034
Entry historyiIntegrated into UniProtKB/TrEMBL: February 1, 1997
Last sequence update: February 1, 1997
Last modified: February 15, 2017
This is version 72 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.