P71017 (GBSB_BACSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alcohol dehydrogenase
EC=1.1.1.1

Gene names

Name:	gbsB
Ordered Locus Names:	BSU31050

Organism

Bacillus subtilis

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	402 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential for the utilization of choline as a precursor.
Catalytic activity	An alcohol + NAD+ = an aldehyde or ketone + NADH.
Pathway	Amine and polyamine biosynthesis; betaine biosynthesis via choline pathway; betaine aldehyde from choline (dehydrogenase route): step 1/1.
Induction	By choline and by high osmolarity in the presence of choline.
Sequence similarities	Belongs to the iron-containing alcohol dehydrogenase family.
Sequence caution	The sequence AAC44365.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Coding sequence diversity	Alternative initiation
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Molecular function	alcohol dehydrogenase (NAD) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]

Isoform Long (identifier: P71017-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Short (identifier: P71017-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-9: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 402	402	Alcohol dehydrogenase		PRO_0000000875
Natural variations
	Alternative sequence	1 – 9	9	Missing in isoform Short.		VSP_018637

Sequences

	Sequence		Length	Mass (Da)
	Isoform Long [UniParc]. Last modified July 15, 1998. Version 2. Checksum: 98B1F3B3FC75748B	FASTA	402	43,421
	10 20 30 40 50 60 MTLNMKVESM QKFHTFEIPT VIKHGIGAIK HTGEEVAALG VSKALLVTDP GIYKAGVADP 70 80 90 100 110 120 VIESLKEAGI EVVLFNKVEP NPPVRLVNEG SELYKKENCN GLVAVGGGSS MDTAKAIGVE 130 140 150 160 170 180 ATHEGSVLDY EAADGKKPLE NRIPPLTTIP TTAGTGSEVT QWAVITDEER EFKFNTGGPL 190 200 210 220 230 240 IAAHLTIIDP ELHVSMPPHV TAMTGIDALA HAIECYTMKF AQPITDAVAL MAIEYAAHYI 250 260 270 280 290 300 KRAFADGEDL EARYGMAQAA MLAGLSYGSE SAGAAHAMSQ TLGGIIPVAH GQCVAAMMGP 310 320 330 340 350 360 VMEYNWKGYP EKFARIAKAF GIDTSKMTTE EAAKASVNWM YDLVEDLEVP TLEEQGVSPD 370 380 390 400 MIERLSKEAM KDPQTFGNPR DLNEKAYNWI YKRCFNLTPK TV
	Isoform Short [UniParc]. Checksum: 1CCB2529C3FF9AE8	FASTA	393	42,386
	10 20 30 40 50 60 MQKFHTFEIP TVIKHGIGAI KHTGEEVAAL GVSKALLVTD PGIYKAGVAD PVIESLKEAG 70 80 90 100 110 120 IEVVLFNKVE PNPPVRLVNE GSELYKKENC NGLVAVGGGS SMDTAKAIGV EATHEGSVLD 130 140 150 160 170 180 YEAADGKKPL ENRIPPLTTI PTTAGTGSEV TQWAVITDEE REFKFNTGGP LIAAHLTIID 190 200 210 220 230 240 PELHVSMPPH VTAMTGIDAL AHAIECYTMK FAQPITDAVA LMAIEYAAHY IKRAFADGED 250 260 270 280 290 300 LEARYGMAQA AMLAGLSYGS ESAGAAHAMS QTLGGIIPVA HGQCVAAMMG PVMEYNWKGY 310 320 330 340 350 360 PEKFARIAKA FGIDTSKMTT EEAAKASVNW MYDLVEDLEV PTLEEQGVSP DMIERLSKEA 370 380 390 MKDPQTFGNP RDLNEKAYNW IYKRCFNLTP KTV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Synthesis of the osmoprotectant glycine betaine in Bacillus subtilis: characterization of the gbsAB genes." Boch J., Kempf B., Schmid R., Bremer E. J. Bacteriol. 178:5121-5129(1996) [PubMed: 8752328] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF N-TERMINUS. Strain: 168 / JH642.
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[3]	"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later." Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A. Microbiology 155:1758-1775(2009) [PubMed: 19383706] [Abstract] Cited for: SEQUENCE REVISION TO N-TERMINUS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U47861 Genomic DNA. Translation: AAC44365.1. Different initiation. AL009126 Genomic DNA. Translation: CAB15083.2.
PIR	B69629.
RefSeq	NP_390983.2. NC_000964.3.
3D structure databases
ProteinModelPortal	P71017.
SMR	P71017. Positions 14-395.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	938832.
GenomeReviews	Gene locus BSU31050 in contig AL009126_GR.
KEGG	bsu:BSU31050.
NMPDR	fig|224308.1.peg.3108.
PATRIC	18978164. VBIBacSub10457_3246.
Organism-specific databases
GenoList	BSU31050. [Micado]
Phylogenomic databases
GeneTree	EBGT00050000000021.
HOGENOM	HBG742415.
OMA	IDPELHV.
PhylomeDB	P71017.
ProtClustDB	CLSK887778.
Enzyme and pathway databases
BioCyc	BSUB:BSU31050-MONOMER. MetaCyc:MONOMER-8601.
Family and domain databases
InterPro	IPR001670. ADH_Fe. IPR018211. ADH_Fe_CS. [Graphical view]
KO	K11440.
Pfam	PF00465. Fe-ADH. 1 hit. [Graphical view]
PROSITE	PS00913. ADH_IRON_1. 1 hit. PS00060. ADH_IRON_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GBSB_BACSU

Accession

Primary (citable) accession number: P71017

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	July 15, 1998
Last modified:	January 25, 2012
This is version 86 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program