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Protein

Alcohol dehydrogenase

Gene

gbsB

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Essential for the utilization of choline as a precursor.1 Publication

Catalytic activityi

An alcohol + NAD+ = an aldehyde or ketone + NADH.

Pathwayi: betaine biosynthesis via choline pathway

This protein is involved in step 1 of the subpathway that synthesizes betaine aldehyde from choline (dehydrogenase route).1 Publication
Proteins known to be involved in this subpathway in this organism are:
  1. Alcohol dehydrogenase (gbsB)
This subpathway is part of the pathway betaine biosynthesis via choline pathway, which is itself part of Amine and polyamine biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes betaine aldehyde from choline (dehydrogenase route), the pathway betaine biosynthesis via choline pathway and in Amine and polyamine biosynthesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
LigandNAD

Enzyme and pathway databases

BioCyciBSUB:BSU31050-MONOMER.
MetaCyc:MONOMER-8601.
UniPathwayiUPA00529; UER00587.

Names & Taxonomyi

Protein namesi
Recommended name:
Alcohol dehydrogenase (EC:1.1.1.1)
Gene namesi
Name:gbsB
Ordered Locus Names:BSU31050
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000008751 – 402Alcohol dehydrogenaseAdd BLAST402

Proteomic databases

PaxDbiP71017.

Expressioni

Inductioni

By choline and by high osmolarity in the presence of choline. Repressed by GbsR.2 Publications

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100016886.

Structurei

3D structure databases

ProteinModelPortaliP71017.
SMRiP71017.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4108JB8. Bacteria.
COG1454. LUCA.
HOGENOMiHOG000243333.
InParanoidiP71017.
KOiK11440.
OMAiRAACQCP.
PhylomeDBiP71017.

Family and domain databases

InterProiView protein in InterPro
IPR001670. ADH_Fe.
IPR018211. ADH_Fe_CS.
PfamiView protein in Pfam
PF00465. Fe-ADH. 1 hit.
PROSITEiView protein in PROSITE
PS00913. ADH_IRON_1. 1 hit.
PS00060. ADH_IRON_2. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform Long (identifier: P71017-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTLNMKVESM QKFHTFEIPT VIKHGIGAIK HTGEEVAALG VSKALLVTDP
60 70 80 90 100
GIYKAGVADP VIESLKEAGI EVVLFNKVEP NPPVRLVNEG SELYKKENCN
110 120 130 140 150
GLVAVGGGSS MDTAKAIGVE ATHEGSVLDY EAADGKKPLE NRIPPLTTIP
160 170 180 190 200
TTAGTGSEVT QWAVITDEER EFKFNTGGPL IAAHLTIIDP ELHVSMPPHV
210 220 230 240 250
TAMTGIDALA HAIECYTMKF AQPITDAVAL MAIEYAAHYI KRAFADGEDL
260 270 280 290 300
EARYGMAQAA MLAGLSYGSE SAGAAHAMSQ TLGGIIPVAH GQCVAAMMGP
310 320 330 340 350
VMEYNWKGYP EKFARIAKAF GIDTSKMTTE EAAKASVNWM YDLVEDLEVP
360 370 380 390 400
TLEEQGVSPD MIERLSKEAM KDPQTFGNPR DLNEKAYNWI YKRCFNLTPK

TV
Length:402
Mass (Da):43,421
Last modified:July 15, 1998 - v2
Checksum:i98B1F3B3FC75748B
GO
Isoform Short (identifier: P71017-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-9: Missing.

Show »
Length:393
Mass (Da):42,386
Checksum:i1CCB2529C3FF9AE8
GO

Sequence cautioni

The sequence AAC44365 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0186371 – 9Missing in isoform Short. Curated9

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U47861 Genomic DNA. Translation: AAC44365.1. Different initiation.
AL009126 Genomic DNA. Translation: CAB15083.2.
PIRiB69629.
RefSeqiNP_390983.2. NC_000964.3. [P71017-1]
WP_003243227.1. NZ_JNCM01000033.1.

Genome annotation databases

EnsemblBacteriaiCAB15083; CAB15083; BSU31050.
GeneIDi938832.
KEGGibsu:BSU31050.
PATRICifig|224308.179.peg.3365.

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U47861 Genomic DNA. Translation: AAC44365.1. Different initiation.
AL009126 Genomic DNA. Translation: CAB15083.2.
PIRiB69629.
RefSeqiNP_390983.2. NC_000964.3. [P71017-1]
WP_003243227.1. NZ_JNCM01000033.1.

3D structure databases

ProteinModelPortaliP71017.
SMRiP71017.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100016886.

Proteomic databases

PaxDbiP71017.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB15083; CAB15083; BSU31050.
GeneIDi938832.
KEGGibsu:BSU31050.
PATRICifig|224308.179.peg.3365.

Phylogenomic databases

eggNOGiENOG4108JB8. Bacteria.
COG1454. LUCA.
HOGENOMiHOG000243333.
InParanoidiP71017.
KOiK11440.
OMAiRAACQCP.
PhylomeDBiP71017.

Enzyme and pathway databases

UniPathwayiUPA00529; UER00587.
BioCyciBSUB:BSU31050-MONOMER.
MetaCyc:MONOMER-8601.

Family and domain databases

InterProiView protein in InterPro
IPR001670. ADH_Fe.
IPR018211. ADH_Fe_CS.
PfamiView protein in Pfam
PF00465. Fe-ADH. 1 hit.
PROSITEiView protein in PROSITE
PS00913. ADH_IRON_1. 1 hit.
PS00060. ADH_IRON_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGBSB_BACSU
AccessioniPrimary (citable) accession number: P71017
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 15, 1998
Last modified: June 7, 2017
This is version 113 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.