Reviewed, UniProtKB/Swiss-Prot P71016 (BETB_BACSU)

Names and origin

Protein names

Recommended name:
    Betaine aldehyde dehydrogenase
      Short name=BADH
    EC=1.2.1.8

Gene names

Name:	gbsA
Ordered Locus Names:	BSU31060

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	490 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This is a soluble NAD-dependent betaine aldehyde-specific dehydrogenase. Essential for the utilization of choline as a precursor.
Catalytic activity	Betaine aldehyde + NAD(+) + H(2)O = betaine + NADH.
Pathway	Amine and polyamine biosynthesis; betaine biosynthesis via choline pathway; betaine from betaine aldehyde: step 1/1.
Induction	By choline and by high osmolarity in the presence of choline.
Sequence similarities	Belongs to the aldehyde dehydrogenase family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	betaine biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	betaine-aldehyde dehydrogenase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 490	490	Betaine aldehyde dehydrogenase		PRO_0000056533
Regions
	Nucleotide binding	227 – 232	6	NAD By similarity
Sites
	Active site	249	1	By similarity
	Active site	283	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P71016-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 67F44C5818B4AD55	FASTA	490	53,666
	10 20 30 40 50 60 MSQTLFIDGE WISAEKEQIR SIINPFNQEE IATVSEGGRE DAIKAIAAAR RAFDKGEWSS 70 80 90 100 110 120 LSGLERGKIV LKIAELIRRD LEELAELESL DTGKTLEESK ADMDDIANVF QYYAGLADKD 130 140 150 160 170 180 GGEIISSPIP DSESKIIREP IGVCGQITPW NYPLLQASWK IAPALAAGNT IVMKPSEITP 190 200 210 220 230 240 LTTIKVFKLM EEAGVPKGVA NLVLGPGATV GDELAVNKDV DLISFTGGIE TGKKIMRAAS 250 260 270 280 290 300 GNVKKIALEL GGKNPNIVFK DADLEVAVDQ ALNAVFFHAG QVCSAGSRLL VEDAIHDQFL 310 320 330 340 350 360 AELVKRAKRI KLGNGFHAET ESGPLISAEH RAKVEKYVEI GIEEGAKLET GGKRPEDPEL 370 380 390 400 410 420 QNGFFYEPTI FSNCNSDMRI VQEEVFGPVL TVETFSSEEE VIELANDTIY GLAGAVWSKD 430 440 450 460 470 480 IEKCERVAAR LRMGTVWIND FHPYFAQAPW GGYKQSGFGR ELGKIGLEEY TEVKHVYRNT 490 KPAAVNWFNS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Synthesis of the osmoprotectant glycine betaine in Bacillus subtilis: characterization of the gbsAB genes." Boch J., Kempf B., Schmid R., Bremer E. J. Bacteriol. 178:5121-5129(1996) [PubMed: 8752328] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-25. Strain: 168 / JH642.
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U47861 Genomic DNA. Translation: AAC44364.1. Z99119 Genomic DNA. Translation: CAB15084.1.
PIR	A69629.
RefSeq	NP_390984.1.
3D structure databases
HSSP	HSSP built from PDB template 1O04 based on UniProtKB P05091.
ModBase	Search...
Genome annotation databases
GeneID	938829.
GenomeReviews	Gene locus BSU31060 in contig AL009126_GR.
KEGG	bsu:BSU31060.
NMPDR	fig|224308.1.peg.3109.
Organism-specific databases
SubtiList	BG11940. gbsA. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	P71016.
Enzyme and pathway databases
BioCyc	BSUB224308:BSU3101-MON. MetaCyc:MON-8602.
Family and domain databases
InterPro	IPR016160. Ald_DHase_CS. IPR016162. Ald_DHase_N. IPR015590. Aldehyde_DHase. IPR011264. Betaine_Ald_DHase. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
TIGRFAMs	TIGR01804. BADH. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BETB_BACSU

Accession

Primary (citable) accession number: P71016

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	November 25, 2008
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)