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Protein

Betaine aldehyde dehydrogenase

Gene

gbsA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

This is a soluble NAD-dependent betaine aldehyde-specific dehydrogenase. Essential for the utilization of choline as a precursor.1 Publication

Catalytic activityi

Betaine aldehyde + NAD+ + H2O = betaine + NADH.

Pathway: betaine biosynthesis via choline pathway

This protein is involved in step 1 of the subpathway that synthesizes betaine from betaine aldehyde.1 Publication
Proteins known to be involved in this subpathway in this organism are:
  1. Betaine aldehyde dehydrogenase (gbsA)
This subpathway is part of the pathway betaine biosynthesis via choline pathway, which is itself part of Amine and polyamine biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes betaine from betaine aldehyde, the pathway betaine biosynthesis via choline pathway and in Amine and polyamine biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei249 – 2491By similarity
Active sitei283 – 2831By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi227 – 2326NADBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciBSUB:BSU31060-MONOMER.
MetaCyc:MONOMER-8602.
UniPathwayiUPA00529; UER00386.

Names & Taxonomyi

Protein namesi
Recommended name:
Betaine aldehyde dehydrogenase (EC:1.2.1.8)
Short name:
BADH
Gene namesi
Name:gbsA
Ordered Locus Names:BSU31060
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570 Componenti: Chromosome

Organism-specific databases

GenoListiBSU31060. [Micado]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 490490Betaine aldehyde dehydrogenasePRO_0000056533Add
BLAST

Proteomic databases

PaxDbiP71016.

Expressioni

Inductioni

By choline and by high osmolarity in the presence of choline. Repressed by GbsR.2 Publications

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100016891.

Structurei

3D structure databases

ProteinModelPortaliP71016.
SMRiP71016. Positions 1-489.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aldehyde dehydrogenase family.Curated

Phylogenomic databases

eggNOGiCOG1012.
HOGENOMiHOG000271505.
InParanoidiP71016.
KOiK00130.
OMAiLSACFLN.
OrthoDBiEOG6BS8QW.
PhylomeDBiP71016.

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR011264. BADH.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMiSSF53720. SSF53720. 1 hit.
TIGRFAMsiTIGR01804. BADH. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P71016-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQTLFIDGE WISAEKEQIR SIINPFNQEE IATVSEGGRE DAIKAIAAAR
60 70 80 90 100
RAFDKGEWSS LSGLERGKIV LKIAELIRRD LEELAELESL DTGKTLEESK
110 120 130 140 150
ADMDDIANVF QYYAGLADKD GGEIISSPIP DSESKIIREP IGVCGQITPW
160 170 180 190 200
NYPLLQASWK IAPALAAGNT IVMKPSEITP LTTIKVFKLM EEAGVPKGVA
210 220 230 240 250
NLVLGPGATV GDELAVNKDV DLISFTGGIE TGKKIMRAAS GNVKKIALEL
260 270 280 290 300
GGKNPNIVFK DADLEVAVDQ ALNAVFFHAG QVCSAGSRLL VEDAIHDQFL
310 320 330 340 350
AELVKRAKRI KLGNGFHAET ESGPLISAEH RAKVEKYVEI GIEEGAKLET
360 370 380 390 400
GGKRPEDPEL QNGFFYEPTI FSNCNSDMRI VQEEVFGPVL TVETFSSEEE
410 420 430 440 450
VIELANDTIY GLAGAVWSKD IEKCERVAAR LRMGTVWIND FHPYFAQAPW
460 470 480 490
GGYKQSGFGR ELGKIGLEEY TEVKHVYRNT KPAAVNWFNS
Length:490
Mass (Da):53,666
Last modified:February 1, 1997 - v1
Checksum:i67F44C5818B4AD55
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U47861 Genomic DNA. Translation: AAC44364.1.
AL009126 Genomic DNA. Translation: CAB15084.1.
PIRiA69629.
RefSeqiNP_390984.1. NC_000964.3.
WP_003243430.1. NZ_JNCM01000033.1.

Genome annotation databases

EnsemblBacteriaiCAB15084; CAB15084; BSU31060.
GeneIDi938829.
KEGGibsu:BSU31060.
PATRICi18978166. VBIBacSub10457_3247.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U47861 Genomic DNA. Translation: AAC44364.1.
AL009126 Genomic DNA. Translation: CAB15084.1.
PIRiA69629.
RefSeqiNP_390984.1. NC_000964.3.
WP_003243430.1. NZ_JNCM01000033.1.

3D structure databases

ProteinModelPortaliP71016.
SMRiP71016. Positions 1-489.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100016891.

Proteomic databases

PaxDbiP71016.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB15084; CAB15084; BSU31060.
GeneIDi938829.
KEGGibsu:BSU31060.
PATRICi18978166. VBIBacSub10457_3247.

Organism-specific databases

GenoListiBSU31060. [Micado]

Phylogenomic databases

eggNOGiCOG1012.
HOGENOMiHOG000271505.
InParanoidiP71016.
KOiK00130.
OMAiLSACFLN.
OrthoDBiEOG6BS8QW.
PhylomeDBiP71016.

Enzyme and pathway databases

UniPathwayiUPA00529; UER00386.
BioCyciBSUB:BSU31060-MONOMER.
MetaCyc:MONOMER-8602.

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR011264. BADH.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMiSSF53720. SSF53720. 1 hit.
TIGRFAMsiTIGR01804. BADH. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Synthesis of the osmoprotectant glycine betaine in Bacillus subtilis: characterization of the gbsAB genes."
    Boch J., Kempf B., Schmid R., Bremer E.
    J. Bacteriol. 178:5121-5129(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-25, FUNCTION, PATHWAY, INDUCTION.
    Strain: 168 / JH642.
  2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  3. "Genetic control of osmoadaptive glycine betaine synthesis in Bacillus subtilis through the choline-sensing and glycine betaine-responsive GbsR repressor."
    Nau-Wagner G., Opper D., Rolbetzki A., Boch J., Kempf B., Hoffmann T., Bremer E.
    J. Bacteriol. 194:2703-2714(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
    Strain: 168 / JH642.

Entry informationi

Entry nameiBETB_BACSU
AccessioniPrimary (citable) accession number: P71016
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: June 24, 2015
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.