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P71016

- BETB_BACSU

UniProt

P71016 - BETB_BACSU

Protein

Betaine aldehyde dehydrogenase

Gene

gbsA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
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    Functioni

    This is a soluble NAD-dependent betaine aldehyde-specific dehydrogenase. Essential for the utilization of choline as a precursor.1 Publication

    Catalytic activityi

    Betaine aldehyde + NAD+ + H2O = betaine + NADH.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei249 – 2491By similarity
    Active sitei283 – 2831By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi227 – 2326NADBy similarity

    GO - Molecular functioni

    1. betaine-aldehyde dehydrogenase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glycine betaine biosynthetic process from choline Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BioCyciBSUB:BSU31060-MONOMER.
    MetaCyc:MONOMER-8602.
    UniPathwayiUPA00529; UER00386.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Betaine aldehyde dehydrogenase (EC:1.2.1.8)
    Short name:
    BADH
    Gene namesi
    Name:gbsA
    Ordered Locus Names:BSU31060
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570: Chromosome

    Organism-specific databases

    GenoListiBSU31060. [Micado]

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 490490Betaine aldehyde dehydrogenasePRO_0000056533Add
    BLAST

    Proteomic databases

    PaxDbiP71016.

    Expressioni

    Inductioni

    By choline and by high osmolarity in the presence of choline. Repressed by GbsR.2 Publications

    Interactioni

    Protein-protein interaction databases

    STRINGi224308.BSU31060.

    Structurei

    3D structure databases

    ProteinModelPortaliP71016.
    SMRiP71016. Positions 1-489.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the aldehyde dehydrogenase family.Curated

    Phylogenomic databases

    eggNOGiCOG1012.
    HOGENOMiHOG000271505.
    KOiK00130.
    OMAiFGPVVSM.
    OrthoDBiEOG6BS8QW.
    PhylomeDBiP71016.

    Family and domain databases

    Gene3Di3.40.309.10. 1 hit.
    3.40.605.10. 1 hit.
    InterProiIPR016161. Ald_DH/histidinol_DH.
    IPR016163. Ald_DH_C.
    IPR016160. Ald_DH_CS_CYS.
    IPR029510. Ald_DH_CS_GLU.
    IPR016162. Ald_DH_N.
    IPR015590. Aldehyde_DH_dom.
    IPR011264. BADH.
    [Graphical view]
    PfamiPF00171. Aldedh. 1 hit.
    [Graphical view]
    SUPFAMiSSF53720. SSF53720. 1 hit.
    TIGRFAMsiTIGR01804. BADH. 1 hit.
    PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
    PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P71016-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSQTLFIDGE WISAEKEQIR SIINPFNQEE IATVSEGGRE DAIKAIAAAR    50
    RAFDKGEWSS LSGLERGKIV LKIAELIRRD LEELAELESL DTGKTLEESK 100
    ADMDDIANVF QYYAGLADKD GGEIISSPIP DSESKIIREP IGVCGQITPW 150
    NYPLLQASWK IAPALAAGNT IVMKPSEITP LTTIKVFKLM EEAGVPKGVA 200
    NLVLGPGATV GDELAVNKDV DLISFTGGIE TGKKIMRAAS GNVKKIALEL 250
    GGKNPNIVFK DADLEVAVDQ ALNAVFFHAG QVCSAGSRLL VEDAIHDQFL 300
    AELVKRAKRI KLGNGFHAET ESGPLISAEH RAKVEKYVEI GIEEGAKLET 350
    GGKRPEDPEL QNGFFYEPTI FSNCNSDMRI VQEEVFGPVL TVETFSSEEE 400
    VIELANDTIY GLAGAVWSKD IEKCERVAAR LRMGTVWIND FHPYFAQAPW 450
    GGYKQSGFGR ELGKIGLEEY TEVKHVYRNT KPAAVNWFNS 490
    Length:490
    Mass (Da):53,666
    Last modified:February 1, 1997 - v1
    Checksum:i67F44C5818B4AD55
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U47861 Genomic DNA. Translation: AAC44364.1.
    AL009126 Genomic DNA. Translation: CAB15084.1.
    PIRiA69629.
    RefSeqiNP_390984.1. NC_000964.3.

    Genome annotation databases

    EnsemblBacteriaiCAB15084; CAB15084; BSU31060.
    GeneIDi938829.
    KEGGibsu:BSU31060.
    PATRICi18978166. VBIBacSub10457_3247.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U47861 Genomic DNA. Translation: AAC44364.1 .
    AL009126 Genomic DNA. Translation: CAB15084.1 .
    PIRi A69629.
    RefSeqi NP_390984.1. NC_000964.3.

    3D structure databases

    ProteinModelPortali P71016.
    SMRi P71016. Positions 1-489.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224308.BSU31060.

    Proteomic databases

    PaxDbi P71016.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB15084 ; CAB15084 ; BSU31060 .
    GeneIDi 938829.
    KEGGi bsu:BSU31060.
    PATRICi 18978166. VBIBacSub10457_3247.

    Organism-specific databases

    GenoListi BSU31060. [Micado ]

    Phylogenomic databases

    eggNOGi COG1012.
    HOGENOMi HOG000271505.
    KOi K00130.
    OMAi FGPVVSM.
    OrthoDBi EOG6BS8QW.
    PhylomeDBi P71016.

    Enzyme and pathway databases

    UniPathwayi UPA00529 ; UER00386 .
    BioCyci BSUB:BSU31060-MONOMER.
    MetaCyc:MONOMER-8602.

    Family and domain databases

    Gene3Di 3.40.309.10. 1 hit.
    3.40.605.10. 1 hit.
    InterProi IPR016161. Ald_DH/histidinol_DH.
    IPR016163. Ald_DH_C.
    IPR016160. Ald_DH_CS_CYS.
    IPR029510. Ald_DH_CS_GLU.
    IPR016162. Ald_DH_N.
    IPR015590. Aldehyde_DH_dom.
    IPR011264. BADH.
    [Graphical view ]
    Pfami PF00171. Aldedh. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53720. SSF53720. 1 hit.
    TIGRFAMsi TIGR01804. BADH. 1 hit.
    PROSITEi PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
    PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Synthesis of the osmoprotectant glycine betaine in Bacillus subtilis: characterization of the gbsAB genes."
      Boch J., Kempf B., Schmid R., Bremer E.
      J. Bacteriol. 178:5121-5129(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-25, FUNCTION, PATHWAY, INDUCTION.
      Strain: 168 / JH642.
    2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.
    3. "Genetic control of osmoadaptive glycine betaine synthesis in Bacillus subtilis through the choline-sensing and glycine betaine-responsive GbsR repressor."
      Nau-Wagner G., Opper D., Rolbetzki A., Boch J., Kempf B., Hoffmann T., Bremer E.
      J. Bacteriol. 194:2703-2714(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
      Strain: 168 / JH642.

    Entry informationi

    Entry nameiBETB_BACSU
    AccessioniPrimary (citable) accession number: P71016
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 105 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3