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Protein

PTS system fructose-specific EIIABC component

Gene

fruA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in fructose transport.By similarity

Catalytic activityi

[Protein]-N(pi)-phospho-L-histidine + D-fructose(Side 1) = [protein]-L-histidine + D-fructose 1-phosphate(Side 2).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei67 – 671Tele-phosphohistidine intermediate; for EIIA activityPROSITE-ProRule annotation
Active sitei178 – 1781Phosphocysteine intermediate; for EIIB activityBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Phosphotransferase system, Sugar transport, Transport

Enzyme and pathway databases

BioCyciBSUB:BSU14400-MONOMER.

Protein family/group databases

TCDBi4.A.2.1.4. the pts fructose-mannitol (fru) family.

Names & Taxonomyi

Protein namesi
Recommended name:
PTS system fructose-specific EIIABC component
Alternative name(s):
EIIABC-Fru
Including the following 3 domains:
Fructose-specific phosphotransferase enzyme IIA component
Alternative name(s):
EII-Fru
PTS system fructose-specific EIIA component
Fructose-specific phosphotransferase enzyme IIB component (EC:2.7.1.202)
Alternative name(s):
EIII-Fru
PTS system fructose-specific EIIB component
Fructose permease IIC component
Alternative name(s):
PTS system fructose-specific EIIC component
Gene namesi
Name:fruA
Ordered Locus Names:BSU14400
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

  • Cell membrane PROSITE-ProRule annotation; Multi-pass membrane protein PROSITE-ProRule annotation

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei312 – 33221HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei350 – 37021HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei392 – 41221HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei428 – 44821HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei470 – 49021HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei511 – 53121HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei544 – 56421HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei569 – 58921HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei608 – 62821HelicalPROSITE-ProRule annotationAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 635635PTS system fructose-specific EIIABC componentPRO_0000360665Add
BLAST

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP71012.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
ykoTO347553EBI-5242378,EBI-5242987

Protein-protein interaction databases

IntActiP71012. 39 interactions.
STRINGi224308.Bsubs1_010100007986.

Structurei

Secondary structure

1
635
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi172 – 1776Combined sources
Helixi185 – 19915Combined sources
Beta strandi203 – 2097Combined sources
Beta strandi212 – 2154Combined sources
Helixi219 – 2246Combined sources
Beta strandi228 – 2347Combined sources
Helixi239 – 2413Combined sources
Beta strandi244 – 2496Combined sources
Helixi251 – 2566Combined sources
Helixi258 – 2669Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2R4QX-ray1.60A171-273[»]
ProteinModelPortaliP71012.
SMRiP71012. Positions 3-147, 170-273.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP71012.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 149145PTS EIIA type-2PROSITE-ProRule annotationAdd
BLAST
Domaini172 – 26796PTS EIIB type-2PROSITE-ProRule annotationAdd
BLAST
Domaini301 – 635335PTS EIIC type-2PROSITE-ProRule annotationAdd
BLAST

Domaini

The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain.
The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.
The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.

Sequence similaritiesi

Contains 1 PTS EIIA type-2 domain.PROSITE-ProRule annotation
Contains 1 PTS EIIB type-2 domain.PROSITE-ProRule annotation
Contains 1 PTS EIIC type-2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105DM4. Bacteria.
COG1299. LUCA.
COG1445. LUCA.
COG1762. LUCA.
HOGENOMiHOG000227678.
InParanoidiP71012.
KOiK02768.
K02769.
K02770.
OMAiNDRQAYK.
PhylomeDBiP71012.

Family and domain databases

CDDicd05569. PTS_IIB_fructose. 1 hit.
Gene3Di3.40.930.10. 1 hit.
InterProiIPR016152. PTrfase/Anion_transptr.
IPR002178. PTS_EIIA_type-2_dom.
IPR013011. PTS_EIIB_2.
IPR003501. PTS_EIIB_2/3.
IPR003352. PTS_EIIC.
IPR013014. PTS_EIIC_2.
IPR004715. PTS_IIA_fruc.
IPR003353. PTS_IIB_fruc.
IPR006327. PTS_IIC_fruc.
[Graphical view]
PfamiPF00359. PTS_EIIA_2. 1 hit.
PF02378. PTS_EIIC. 1 hit.
PF02302. PTS_IIB. 1 hit.
[Graphical view]
SUPFAMiSSF52794. SSF52794. 1 hit.
SSF55804. SSF55804. 1 hit.
TIGRFAMsiTIGR00829. FRU. 1 hit.
TIGR00848. fruA. 1 hit.
TIGR01427. PTS_IIC_fructo. 1 hit.
PROSITEiPS51094. PTS_EIIA_TYPE_2. 1 hit.
PS00372. PTS_EIIA_TYPE_2_HIS. 1 hit.
PS51099. PTS_EIIB_TYPE_2. 1 hit.
PS51104. PTS_EIIC_TYPE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P71012-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKITELLTKH TIKLNIESKE KENVIDEMVT VLDKAGKLND RQAYKEAILN
60 70 80 90 100
RESQSSTGIG EGIAIPHAKT ASVINPAIAF GRSKDGVDYE SLDGQPAHLV
110 120 130 140 150
FMIAATEGAN NTHLEALSRL STLLMREEIR KQLLEAESED AIIDIINQHD
160 170 180 190 200
KDDDEEEEEE EAAPAPAGKG KILAVTACPT GIAHTFMAAD ALKEKAKELG
210 220 230 240 250
VEIKVETNGS SGIKHKLTAQ EIEDAPAIIV AADKQVEMER FKGKRVLQVP
260 270 280 290 300
VTAGIRRPQE LIEKAMNQDA PIYQGSGGGS AASNDDEEAK GKSGSGIGNT
310 320 330 340 350
FYKHLMSGVS NMLPFVVGGG ILVAISFFWG IHSADPNDPS YNTFAAALNF
360 370 380 390 400
IGGDNALKLI VAVLAGFIAM SIADRPGFAP GMVGGFMATQ ANAGFLGGLI
410 420 430 440 450
AGFLAGYVVI LLKKVFTFIP QSLDGLKPVL IYPLFGIFIT GVLMQFVVNT
460 470 480 490 500
PVAAFMNFLT NWLESLGTGN LVLMGIILGG MMAIDMGGPL NKAAFTFGIA
510 520 530 540 550
MIDAGNYAPH AAIMAGGMVP PLGIALATTI FRNKFTQRDR EAGITCYFMG
560 570 580 590 600
AAFVTEGAIP FAAADPLRVI PAAVVGAAVA GGLTEFFRVT LPAPHGGVFV
610 620 630
AFITNHPMLY LLSIVIGAVV MAIILGIVKK PVTEK
Length:635
Mass (Da):67,184
Last modified:June 1, 1998 - v2
Checksum:i753F12C2AFDD7F84
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF012285 Genomic DNA. Translation: AAC24915.1.
AL009126 Genomic DNA. Translation: CAB13313.1.
PIRiH69626.
RefSeqiNP_389323.1. NC_000964.3.
WP_003232350.1. NZ_JNCM01000035.1.

Genome annotation databases

EnsemblBacteriaiCAB13313; CAB13313; BSU14400.
GeneIDi938757.
KEGGibsu:BSU14400.
PATRICi18974669. VBIBacSub10457_1526.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF012285 Genomic DNA. Translation: AAC24915.1.
AL009126 Genomic DNA. Translation: CAB13313.1.
PIRiH69626.
RefSeqiNP_389323.1. NC_000964.3.
WP_003232350.1. NZ_JNCM01000035.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2R4QX-ray1.60A171-273[»]
ProteinModelPortaliP71012.
SMRiP71012. Positions 3-147, 170-273.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP71012. 39 interactions.
STRINGi224308.Bsubs1_010100007986.

Protein family/group databases

TCDBi4.A.2.1.4. the pts fructose-mannitol (fru) family.

Proteomic databases

PaxDbiP71012.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB13313; CAB13313; BSU14400.
GeneIDi938757.
KEGGibsu:BSU14400.
PATRICi18974669. VBIBacSub10457_1526.

Phylogenomic databases

eggNOGiENOG4105DM4. Bacteria.
COG1299. LUCA.
COG1445. LUCA.
COG1762. LUCA.
HOGENOMiHOG000227678.
InParanoidiP71012.
KOiK02768.
K02769.
K02770.
OMAiNDRQAYK.
PhylomeDBiP71012.

Enzyme and pathway databases

BioCyciBSUB:BSU14400-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP71012.

Family and domain databases

CDDicd05569. PTS_IIB_fructose. 1 hit.
Gene3Di3.40.930.10. 1 hit.
InterProiIPR016152. PTrfase/Anion_transptr.
IPR002178. PTS_EIIA_type-2_dom.
IPR013011. PTS_EIIB_2.
IPR003501. PTS_EIIB_2/3.
IPR003352. PTS_EIIC.
IPR013014. PTS_EIIC_2.
IPR004715. PTS_IIA_fruc.
IPR003353. PTS_IIB_fruc.
IPR006327. PTS_IIC_fruc.
[Graphical view]
PfamiPF00359. PTS_EIIA_2. 1 hit.
PF02378. PTS_EIIC. 1 hit.
PF02302. PTS_IIB. 1 hit.
[Graphical view]
SUPFAMiSSF52794. SSF52794. 1 hit.
SSF55804. SSF55804. 1 hit.
TIGRFAMsiTIGR00829. FRU. 1 hit.
TIGR00848. fruA. 1 hit.
TIGR01427. PTS_IIC_fructo. 1 hit.
PROSITEiPS51094. PTS_EIIA_TYPE_2. 1 hit.
PS00372. PTS_EIIA_TYPE_2_HIS. 1 hit.
PS51099. PTS_EIIB_TYPE_2. 1 hit.
PS51104. PTS_EIIC_TYPE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTF3A_BACSU
AccessioniPrimary (citable) accession number: P71012
Secondary accession number(s): Q7BVR6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: June 1, 1998
Last modified: September 7, 2016
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.