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Protein

PTS system fructose-specific EIIABC component

Gene

fruA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in fructose transport.By similarity

Catalytic activityi

[Protein]-N(pi)-phospho-L-histidine + D-fructose(Side 1) = [protein]-L-histidine + D-fructose 1-phosphate(Side 2).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei67Tele-phosphohistidine intermediate; for EIIA activityPROSITE-ProRule annotation1
Active sitei178Phosphocysteine intermediate; for EIIB activityCurated1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Phosphotransferase system, Sugar transport, Transport

Enzyme and pathway databases

BioCyciBSUB:BSU14400-MONOMER.

Protein family/group databases

TCDBi4.A.2.1.4. the pts fructose-mannitol (fru) family.

Names & Taxonomyi

Protein namesi
Recommended name:
PTS system fructose-specific EIIABC componentBy similarity
Alternative name(s):
EIIABC-FruBy similarity
Including the following 3 domains:
PTS system fructose-specific EIIA componentBy similarity (EC:2.7.1.202By similarity)
Alternative name(s):
EII-FruBy similarity
Fructose-specific phosphotransferase enzyme IIA componentBy similarity
PTS system fructose-specific EIIB componentBy similarity (EC:2.7.1.202By similarity)
Alternative name(s):
EIII-FruBy similarity
Fructose-specific phosphotransferase enzyme IIB componentBy similarity
PTS system fructose-specific EIIC componentBy similarity
Alternative name(s):
Fructose permease IIC componentBy similarity
Gene namesi
Name:fruA
Ordered Locus Names:BSU14400
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

  • Cell membrane PROSITE-ProRule annotation; Multi-pass membrane protein PROSITE-ProRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei312 – 332HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei350 – 370HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei392 – 412HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei428 – 448HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei470 – 490HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei511 – 531HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei544 – 564HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei569 – 589HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei608 – 628HelicalPROSITE-ProRule annotationAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003606651 – 635PTS system fructose-specific EIIABC componentAdd BLAST635

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei67Phosphohistidine; by HPrCurated1
Modified residuei178Phosphocysteine; by EIIAPROSITE-ProRule annotation1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP71012.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
ykoTO347553EBI-5242378,EBI-5242987

Protein-protein interaction databases

IntActiP71012. 39 interactors.
STRINGi224308.Bsubs1_010100007986.

Structurei

Secondary structure

1635
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi172 – 177Combined sources6
Helixi185 – 199Combined sources15
Beta strandi203 – 209Combined sources7
Beta strandi212 – 215Combined sources4
Helixi219 – 224Combined sources6
Beta strandi228 – 234Combined sources7
Helixi239 – 241Combined sources3
Beta strandi244 – 249Combined sources6
Helixi251 – 256Combined sources6
Helixi258 – 266Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2R4QX-ray1.60A171-273[»]
ProteinModelPortaliP71012.
SMRiP71012.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP71012.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 149PTS EIIA type-2PROSITE-ProRule annotationAdd BLAST145
Domaini172 – 267PTS EIIB type-2PROSITE-ProRule annotationAdd BLAST96
Domaini301 – 635PTS EIIC type-2PROSITE-ProRule annotationAdd BLAST335

Domaini

The PTS EIIA type-2 domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the PTS EIIB type-2 domain.PROSITE-ProRule annotation
The PTS EIIB type-2 domain is phosphorylated by phospho-EIIA on a cysteinyl residue. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the PTS EIIC type-2 domain.PROSITE-ProRule annotation
The EIIC type-2 domain forms the PTS system translocation channel and contains the specific substrate-binding site.PROSITE-ProRule annotation

Sequence similaritiesi

Contains 1 PTS EIIA type-2 domain.PROSITE-ProRule annotation
Contains 1 PTS EIIB type-2 domain.PROSITE-ProRule annotation
Contains 1 PTS EIIC type-2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105DM4. Bacteria.
COG1299. LUCA.
COG1445. LUCA.
COG1762. LUCA.
HOGENOMiHOG000227678.
InParanoidiP71012.
KOiK02768.
K02769.
K02770.
OMAiNDRQAYK.
PhylomeDBiP71012.

Family and domain databases

CDDicd05569. PTS_IIB_fructose. 1 hit.
Gene3Di3.40.930.10. 1 hit.
InterProiIPR016152. PTrfase/Anion_transptr.
IPR002178. PTS_EIIA_type-2_dom.
IPR013011. PTS_EIIB_2.
IPR003501. PTS_EIIB_2/3.
IPR003352. PTS_EIIC.
IPR013014. PTS_EIIC_2.
IPR004715. PTS_IIA_fruc.
IPR003353. PTS_IIB_fruc.
IPR006327. PTS_IIC_fruc.
[Graphical view]
PfamiPF00359. PTS_EIIA_2. 1 hit.
PF02378. PTS_EIIC. 1 hit.
PF02302. PTS_IIB. 1 hit.
[Graphical view]
SUPFAMiSSF52794. SSF52794. 1 hit.
SSF55804. SSF55804. 1 hit.
TIGRFAMsiTIGR00829. FRU. 1 hit.
TIGR00848. fruA. 1 hit.
TIGR01427. PTS_IIC_fructo. 1 hit.
PROSITEiPS51094. PTS_EIIA_TYPE_2. 1 hit.
PS00372. PTS_EIIA_TYPE_2_HIS. 1 hit.
PS51099. PTS_EIIB_TYPE_2. 1 hit.
PS51104. PTS_EIIC_TYPE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P71012-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKITELLTKH TIKLNIESKE KENVIDEMVT VLDKAGKLND RQAYKEAILN
60 70 80 90 100
RESQSSTGIG EGIAIPHAKT ASVINPAIAF GRSKDGVDYE SLDGQPAHLV
110 120 130 140 150
FMIAATEGAN NTHLEALSRL STLLMREEIR KQLLEAESED AIIDIINQHD
160 170 180 190 200
KDDDEEEEEE EAAPAPAGKG KILAVTACPT GIAHTFMAAD ALKEKAKELG
210 220 230 240 250
VEIKVETNGS SGIKHKLTAQ EIEDAPAIIV AADKQVEMER FKGKRVLQVP
260 270 280 290 300
VTAGIRRPQE LIEKAMNQDA PIYQGSGGGS AASNDDEEAK GKSGSGIGNT
310 320 330 340 350
FYKHLMSGVS NMLPFVVGGG ILVAISFFWG IHSADPNDPS YNTFAAALNF
360 370 380 390 400
IGGDNALKLI VAVLAGFIAM SIADRPGFAP GMVGGFMATQ ANAGFLGGLI
410 420 430 440 450
AGFLAGYVVI LLKKVFTFIP QSLDGLKPVL IYPLFGIFIT GVLMQFVVNT
460 470 480 490 500
PVAAFMNFLT NWLESLGTGN LVLMGIILGG MMAIDMGGPL NKAAFTFGIA
510 520 530 540 550
MIDAGNYAPH AAIMAGGMVP PLGIALATTI FRNKFTQRDR EAGITCYFMG
560 570 580 590 600
AAFVTEGAIP FAAADPLRVI PAAVVGAAVA GGLTEFFRVT LPAPHGGVFV
610 620 630
AFITNHPMLY LLSIVIGAVV MAIILGIVKK PVTEK
Length:635
Mass (Da):67,184
Last modified:June 1, 1998 - v2
Checksum:i753F12C2AFDD7F84
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF012285 Genomic DNA. Translation: AAC24915.1.
AL009126 Genomic DNA. Translation: CAB13313.1.
PIRiH69626.
RefSeqiNP_389323.1. NC_000964.3.
WP_003232350.1. NZ_JNCM01000035.1.

Genome annotation databases

EnsemblBacteriaiCAB13313; CAB13313; BSU14400.
GeneIDi938757.
KEGGibsu:BSU14400.
PATRICi18974669. VBIBacSub10457_1526.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF012285 Genomic DNA. Translation: AAC24915.1.
AL009126 Genomic DNA. Translation: CAB13313.1.
PIRiH69626.
RefSeqiNP_389323.1. NC_000964.3.
WP_003232350.1. NZ_JNCM01000035.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2R4QX-ray1.60A171-273[»]
ProteinModelPortaliP71012.
SMRiP71012.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP71012. 39 interactors.
STRINGi224308.Bsubs1_010100007986.

Protein family/group databases

TCDBi4.A.2.1.4. the pts fructose-mannitol (fru) family.

Proteomic databases

PaxDbiP71012.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB13313; CAB13313; BSU14400.
GeneIDi938757.
KEGGibsu:BSU14400.
PATRICi18974669. VBIBacSub10457_1526.

Phylogenomic databases

eggNOGiENOG4105DM4. Bacteria.
COG1299. LUCA.
COG1445. LUCA.
COG1762. LUCA.
HOGENOMiHOG000227678.
InParanoidiP71012.
KOiK02768.
K02769.
K02770.
OMAiNDRQAYK.
PhylomeDBiP71012.

Enzyme and pathway databases

BioCyciBSUB:BSU14400-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP71012.

Family and domain databases

CDDicd05569. PTS_IIB_fructose. 1 hit.
Gene3Di3.40.930.10. 1 hit.
InterProiIPR016152. PTrfase/Anion_transptr.
IPR002178. PTS_EIIA_type-2_dom.
IPR013011. PTS_EIIB_2.
IPR003501. PTS_EIIB_2/3.
IPR003352. PTS_EIIC.
IPR013014. PTS_EIIC_2.
IPR004715. PTS_IIA_fruc.
IPR003353. PTS_IIB_fruc.
IPR006327. PTS_IIC_fruc.
[Graphical view]
PfamiPF00359. PTS_EIIA_2. 1 hit.
PF02378. PTS_EIIC. 1 hit.
PF02302. PTS_IIB. 1 hit.
[Graphical view]
SUPFAMiSSF52794. SSF52794. 1 hit.
SSF55804. SSF55804. 1 hit.
TIGRFAMsiTIGR00829. FRU. 1 hit.
TIGR00848. fruA. 1 hit.
TIGR01427. PTS_IIC_fructo. 1 hit.
PROSITEiPS51094. PTS_EIIA_TYPE_2. 1 hit.
PS00372. PTS_EIIA_TYPE_2_HIS. 1 hit.
PS51099. PTS_EIIB_TYPE_2. 1 hit.
PS51104. PTS_EIIC_TYPE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTF3A_BACSU
AccessioniPrimary (citable) accession number: P71012
Secondary accession number(s): Q7BVR6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: June 1, 1998
Last modified: November 2, 2016
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.