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Protein

Polyamine aminopropyltransferase

Gene

speE

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the cell growth and proliferation. Catalyzes the irreversible transfer of a propylamine group from the amino donor S-adenosylmethioninamine (decarboxy-AdoMet) to putrescine (1,4-diaminobutane) to yield spermidine (Probable).1 Publication

Catalytic activityi

S-adenosyl 3-(methylthio)propylamine + putrescine = 5'-S-methyl-5'-thioadenosine + spermidine.UniRule annotation

Pathwayi: spermidine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes spermidine from putrescine.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Polyamine aminopropyltransferase (speE)
This subpathway is part of the pathway spermidine biosynthesis, which is itself part of Amine and polyamine biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes spermidine from putrescine, the pathway spermidine biosynthesis and in Amine and polyamine biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei8 – 81PolyamineUniRule annotation
Binding sitei32 – 321S-adenosylmethioninamineUniRule annotation
Binding sitei63 – 631PolyamineUniRule annotation
Binding sitei87 – 871PolyamineUniRule annotation
Binding sitei107 – 1071S-adenosylmethioninamineUniRule annotation
Active sitei156 – 1561Proton acceptorUniRule annotation
Binding sitei163 – 1631S-adenosylmethioninamine; via carbonyl oxygenUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Polyamine biosynthesis, Spermidine biosynthesis

Enzyme and pathway databases

BioCyciBSUB:BSU37500-MONOMER.
UniPathwayiUPA00248; UER00314.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyamine aminopropyltransferaseUniRule annotation
Alternative name(s):
Putrescine aminopropyltransferaseUniRule annotation
Short name:
PAPTUniRule annotation
Spermidine synthaseUniRule annotation (EC:2.5.1.16UniRule annotation)
Short name:
SPDSUniRule annotation
Short name:
SPDSYUniRule annotation
Gene namesi
Name:speEUniRule annotation
Synonyms:ywhF
Ordered Locus Names:BSU37500
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 276276Polyamine aminopropyltransferasePRO_0000156470Add
BLAST

Proteomic databases

PaxDbiP70998.

Interactioni

Subunit structurei

Homodimer or homotetramer.UniRule annotation1 Publication

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100020261.

Structurei

Secondary structure

1
276
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 118Combined sources
Beta strandi14 – 2815Combined sources
Beta strandi33 – 397Combined sources
Turni40 – 423Combined sources
Beta strandi43 – 486Combined sources
Beta strandi51 – 555Combined sources
Turni56 – 594Combined sources
Helixi60 – 7314Combined sources
Beta strandi74 – 763Combined sources
Beta strandi79 – 846Combined sources
Helixi89 – 946Combined sources
Beta strandi101 – 1088Combined sources
Helixi110 – 11910Combined sources
Helixi121 – 1244Combined sources
Turni125 – 1284Combined sources
Beta strandi132 – 1376Combined sources
Helixi140 – 1445Combined sources
Beta strandi150 – 1567Combined sources
Helixi171 – 1799Combined sources
Beta strandi180 – 18910Combined sources
Turni193 – 1953Combined sources
Helixi197 – 20812Combined sources
Beta strandi212 – 2198Combined sources
Helixi225 – 2273Combined sources
Beta strandi229 – 2379Combined sources
Helixi245 – 2473Combined sources
Helixi258 – 2636Combined sources
Helixi269 – 2724Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1IY9X-ray2.30A/B/C/D2-276[»]
ProteinModelPortaliP70998.
SMRiP70998. Positions 2-275.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP70998.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 236234PABSUniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni138 – 1392S-adenosylmethioninamine bindingUniRule annotation
Regioni157 – 1593Polyamine bindingUniRule annotation

Sequence similaritiesi

Belongs to the spermidine/spermine synthase family.UniRule annotation
Contains 1 PABS (polyamine biosynthesis) domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CCX. Bacteria.
COG0421. LUCA.
HOGENOMiHOG000256146.
InParanoidiP70998.
KOiK00797.
OMAiSGLWSFT.
PhylomeDBiP70998.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00198. Spermidine_synth. 1 hit.
InterProiIPR030374. PABS.
IPR030373. PABS_CS.
IPR029063. SAM-dependent_MTases.
IPR001045. Spermi_synthase.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00417. speE. 1 hit.
PROSITEiPS01330. PABS_1. 1 hit.
PS51006. PABS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P70998-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSELWYTEKQ TKNFGITMKV NKTLHTEQTE FQHLEMVETE EFGNMLFLDG
60 70 80 90 100
MVMTSEKDEF VYHEMVAHVP LFTHPNPEHV LVVGGGDGGV IREILKHPSV
110 120 130 140 150
KKATLVDIDG KVIEYSKKFL PSIAGKLDDP RVDVQVDDGF MHIAKSENQY
160 170 180 190 200
DVIMVDSTEP VGPAVNLFTK GFYAGIAKAL KEDGIFVAQT DNPWFTPELI
210 220 230 240 250
TNVQRDVKEI FPITKLYTAN IPTYPSGLWT FTIGSKKYDP LAVEDSRFFD
260 270
IETKYYTKDI HKAAFVLPKF VSDLIK
Length:276
Mass (Da):31,336
Last modified:February 1, 1997 - v1
Checksum:iA5E0725B80FBDD62
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z80360 Genomic DNA. Translation: CAB02516.1.
AL009126 Genomic DNA. Translation: CAB15777.1.
PIRiG70057.
RefSeqiNP_391630.1. NC_000964.3.
WP_003227543.1. NC_000964.3.

Genome annotation databases

EnsemblBacteriaiCAB15777; CAB15777; BSU37500.
GeneIDi937075.
KEGGibsu:BSU37500.
PATRICi18979536. VBIBacSub10457_3930.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z80360 Genomic DNA. Translation: CAB02516.1.
AL009126 Genomic DNA. Translation: CAB15777.1.
PIRiG70057.
RefSeqiNP_391630.1. NC_000964.3.
WP_003227543.1. NC_000964.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1IY9X-ray2.30A/B/C/D2-276[»]
ProteinModelPortaliP70998.
SMRiP70998. Positions 2-275.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100020261.

Proteomic databases

PaxDbiP70998.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB15777; CAB15777; BSU37500.
GeneIDi937075.
KEGGibsu:BSU37500.
PATRICi18979536. VBIBacSub10457_3930.

Phylogenomic databases

eggNOGiENOG4105CCX. Bacteria.
COG0421. LUCA.
HOGENOMiHOG000256146.
InParanoidiP70998.
KOiK00797.
OMAiSGLWSFT.
PhylomeDBiP70998.

Enzyme and pathway databases

UniPathwayiUPA00248; UER00314.
BioCyciBSUB:BSU37500-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP70998.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00198. Spermidine_synth. 1 hit.
InterProiIPR030374. PABS.
IPR030373. PABS_CS.
IPR029063. SAM-dependent_MTases.
IPR001045. Spermi_synthase.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00417. speE. 1 hit.
PROSITEiPS01330. PABS_1. 1 hit.
PS51006. PABS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSPEE_BACSU
AccessioniPrimary (citable) accession number: P70998
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: February 1, 1997
Last modified: September 7, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

B.subtilis has only one biosynthetic pathway from agmatine to spermidine to produce polyamines.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.