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Protein

Penicillin-binding protein 2D

Gene

pbpG

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in the polymerization and cross-linking of spore peptidoglycan. May be required for synthesis of the spore germ cell wall, the first layer of peptidoglycan synthesized on the surface of the inner forespore membrane.2 Publications

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei365Acyl-ester intermediateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Enzyme and pathway databases

BioCyciBSUB:BSU37510-MONOMER.
UniPathwayiUPA00219.

Protein family/group databases

CAZyiGT51. Glycosyltransferase Family 51.

Names & Taxonomyi

Protein namesi
Recommended name:
Penicillin-binding protein 2D
Short name:
PBP-2D
Short name:
PBP2d
Gene namesi
Name:pbpG
Synonyms:ywhE
Ordered Locus Names:BSU37510
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 19CytoplasmicSequence analysisAdd BLAST19
Transmembranei20 – 40Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini41 – 691ExtracellularSequence analysisAdd BLAST651

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype; due to the redundancy with PbpF. Spores have normal heat-resistance, cortex structure, and germination and outgrowth properties.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003601651 – 691Penicillin-binding protein 2DAdd BLAST691

Proteomic databases

PaxDbiP70997.
PRIDEiP70997.

Expressioni

Developmental stagei

Sporulation specific. Expressed predominantly, if not exclusively, in the forespore.1 Publication

Interactioni

Protein-protein interaction databases

IntActiP70997. 1 interactor.
STRINGi224308.Bsubs1_010100020266.

Structurei

3D structure databases

ProteinModelPortaliP70997.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni55 – 223TransglycosylaseAdd BLAST169
Regioni327 – 605TranspeptidaseAdd BLAST279

Sequence similaritiesi

In the N-terminal section; belongs to the glycosyltransferase 51 family.Curated
In the C-terminal section; belongs to the transpeptidase family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105BZ4. Bacteria.
COG0744. LUCA.
HOGENOMiHOG000041137.
InParanoidiP70997.
OMAiPGIKLIR.
PhylomeDBiP70997.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR001264. Glyco_trans_51.
IPR023346. Lysozyme-like_dom.
IPR001460. PCN-bd_Tpept.
[Graphical view]
PfamiPF00912. Transgly. 1 hit.
PF00905. Transpeptidase. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
SSF56601. SSF56601. 2 hits.

Sequencei

Sequence statusi: Complete.

P70997-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDAMTNKRLR LTLKTVRAFI FLGAFAALAA AAVFMTVILI AKYQGAPSVQ
60 70 80 90 100
VPQSTILYAS DGSKLGETNY GEKRYWVPLK DMNPTIVKAT VAIEDQNFYD
110 120 130 140 150
HHGFDYKRMA GAALADLKAF AKVQGASTIT QQYARNLYLE HDKTWKRKWN
160 170 180 190 200
EAFYTIRLEQ NYSKDEILEG YLNTIYYGHG AYGIEAASRL YFGKHAKNLT
210 220 230 240 250
DAEAALLAGI PKGPSGYSPY VNETKAKERQ KTIVRMMEKQ QMISQKKADE
260 270 280 290 300
LIKEPLSYQP LNKQVSKRKA PYFYDNAMRE LEKKLGMTRE QIETSGLNVY
310 320 330 340 350
TTVDKRMQRI AEETITETVN AGSDIQVGFS AIDPRTGNVL ALVGGRDYQK
360 370 380 390 400
SPFDRTTQAK RQPASTIKPL LYYKAIQSGF TPVTLMKSEE TEFQIDAKGE
410 420 430 440 450
TYSPSNYNGY YANKPITLLQ ALALSDNIYA VKTHLFLGTN KLVKTAKEFG
460 470 480 490 500
ITAHLQALPS LALGTEPVRP IEMVNAYAML ANGGKKIEPT FISRVTDAAG
510 520 530 540 550
HVLYENPNQH KQVLDEKAAF VTASMMTGMF DIDLNGYTSV TGRTIANRLT
560 570 580 590 600
RTYAGKSGTT SADSWMIGFN PKLAAGVWTG YDKNSTIDSV EEKSYAKTIW
610 620 630 640 650
ADFMEDALKG EPETAFKPPK GVTGVYIDPE TGYSSGPGCA AKHYTYFVKG
660 670 680 690
TEPANVCYGA EPAKQTKDRL PSKEKPASEK KWWDKWLGRH H
Length:691
Mass (Da):77,037
Last modified:July 28, 2009 - v3
Checksum:i4E9B4CF545CF0C4A
GO

Sequence cautioni

The sequence CAB02515 differs from that shown. Reason: Frameshift at position 638.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z80360 Genomic DNA. Translation: CAB02515.1. Frameshift.
AL009126 Genomic DNA. Translation: CAB15778.2.
PIRiF70057.
RefSeqiNP_391631.2. NC_000964.3.
WP_003227540.1. NZ_JNCM01000034.1.

Genome annotation databases

EnsemblBacteriaiCAB15778; CAB15778; BSU37510.
GeneIDi937200.
KEGGibsu:BSU37510.
PATRICi18979538. VBIBacSub10457_3931.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z80360 Genomic DNA. Translation: CAB02515.1. Frameshift.
AL009126 Genomic DNA. Translation: CAB15778.2.
PIRiF70057.
RefSeqiNP_391631.2. NC_000964.3.
WP_003227540.1. NZ_JNCM01000034.1.

3D structure databases

ProteinModelPortaliP70997.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP70997. 1 interactor.
STRINGi224308.Bsubs1_010100020266.

Protein family/group databases

CAZyiGT51. Glycosyltransferase Family 51.

Proteomic databases

PaxDbiP70997.
PRIDEiP70997.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB15778; CAB15778; BSU37510.
GeneIDi937200.
KEGGibsu:BSU37510.
PATRICi18979538. VBIBacSub10457_3931.

Phylogenomic databases

eggNOGiENOG4105BZ4. Bacteria.
COG0744. LUCA.
HOGENOMiHOG000041137.
InParanoidiP70997.
OMAiPGIKLIR.
PhylomeDBiP70997.

Enzyme and pathway databases

UniPathwayiUPA00219.
BioCyciBSUB:BSU37510-MONOMER.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR001264. Glyco_trans_51.
IPR023346. Lysozyme-like_dom.
IPR001460. PCN-bd_Tpept.
[Graphical view]
PfamiPF00912. Transgly. 1 hit.
PF00905. Transpeptidase. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
SSF56601. SSF56601. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiPBPG_BACSU
AccessioniPrimary (citable) accession number: P70997
Secondary accession number(s): Q794Z1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: July 28, 2009
Last modified: October 5, 2016
This is version 106 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.