Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Modification methylase BsoBI

Gene

bsoBIM

Organism
Geobacillus stearothermophilus (Bacillus stearothermophilus)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence CYCGRG, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the BsoBI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA cytosine = S-adenosyl-L-homocysteine + DNA N(4)-methylcytosine.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BRENDAi2.1.1.113. 623.

Protein family/group databases

REBASEi3311. M.BsoBI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase BsoBI (EC:2.1.1.113)
Short name:
M.BsoBI
Alternative name(s):
N(4)- cytosine-specific methyltransferase BsoBI
Gene namesi
Name:bsoBIM
OrganismiGeobacillus stearothermophilus (Bacillus stearothermophilus)
Taxonomic identifieri1422 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 509509Modification methylase BsoBIPRO_0000087925Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP70986.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.150. 3 hits.
InterProiIPR002941. DNA_methylase_N4/N6.
IPR017985. MeTrfase_CN4_CS.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01555. N6_N4_Mtase. 2 hits.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 4 hits.
PROSITEiPS00093. N4_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P70986-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METVQMSLDL QPEDTKANIE CSSPDYEIKC FSHNQFAPQI ERLKIEKKYI
60 70 80 90 100
HIVEETETFN RKLVSYQANK NQSIHNWIRY KEGFSSELVQ NLIEEFGLSK
110 120 130 140 150
GDTILDPFLG SGTTSLTAKM LGINSIGIDI LPISHIAFEP KSFIFEYNLE
160 170 180 190 200
ELDRAYKEIY EISPTKIEQK FNHLSITEGA FPEETENDLL FFTHWDNNSQ
210 220 230 240 250
YSYQTKTLIK LILVSILEEI SYTRKDGQYL RWDYRSQKVI ETNKKRLEQG
260 270 280 290 300
KEPIKTILDK GELPTVKESL LNTLLTIKED IKEIQQKCLP NESVHELIKD
310 320 330 340 350
SALNALPKIN DNTFDAVITS PPYCNRYDYT RTYALELAYL GVDEKKIREL
360 370 380 390 400
RQAQLSCTVE NKSKLKQLKD YYHSLFLESR YAEIERLVTG NEVLNEINYA
410 420 430 440 450
LRKRWENGEV NNKGILSMVD GYFTELTFIF YELFRTCKPG AKVAFVNDNV
460 470 480 490 500
RYAGEIIPVD FLSTKIAEDI GFKPIKIYTL KQRKGNSSQQ MGKYGRVALR

KSITIWEKP
Length:509
Mass (Da):59,144
Last modified:July 1, 1997 - v2
Checksum:i3C472905C3276D2D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98287 Genomic DNA. Translation: CAA66933.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98287 Genomic DNA. Translation: CAA66933.1.

3D structure databases

ProteinModelPortaliP70986.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi3311. M.BsoBI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi2.1.1.113. 623.

Family and domain databases

Gene3Di3.40.50.150. 3 hits.
InterProiIPR002941. DNA_methylase_N4/N6.
IPR017985. MeTrfase_CN4_CS.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01555. N6_N4_Mtase. 2 hits.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 4 hits.
PROSITEiPS00093. N4_MTASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and sequence comparison of AvaI and BsoBI restriction-modification systems."
    Ruan H., Lunnen K.D., Scott M.E., Moran L.S., Slatko B.E., Pelletier J.J., Hess E.J., Benner J. II, Wilson G.G., Xu S.-Y.
    Mol. Gen. Genet. 252:695-699(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: JN209.

Entry informationi

Entry nameiMTB1_GEOSE
AccessioniPrimary (citable) accession number: P70986
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 8, 2000
Last sequence update: July 1, 1997
Last modified: April 1, 2015
This is version 66 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.