Reviewed, UniProtKB/Swiss-Prot P70986 (MTB1_BACST)

Names and origin

Protein names

Recommended name:
    Modification methylase BsoBI
      Short name=M.BsoBI
    EC=2.1.1.113
Alternative name(s):
    N(4)- cytosine-specific methyltransferase BsoBI

Gene names

Name:

bsoBIM

Organism

Bacillus stearothermophilus (Geobacillus stearothermophilus)

Taxonomic identifier

1422 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length	509 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence CYCGRG, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the BsoBI endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA cytosine = S-adenosyl-L-homocysteine + DNA N(4)-methylcytosine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	DNA methylation on cytosine Inferred from electronic annotation. Source: InterPro DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro N-methyltransferase activity Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (cytosine-N4-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 509	509	Modification methylase BsoBI		PRO_0000087925

Sequences

	Sequence		Length	Mass (Da)
	P70986-1 [UniParc]. Last modified July 1, 1997. Version 2. Checksum: 3C472905C3276D2D	FASTA	509	59,144
	10 20 30 40 50 60 METVQMSLDL QPEDTKANIE CSSPDYEIKC FSHNQFAPQI ERLKIEKKYI HIVEETETFN 70 80 90 100 110 120 RKLVSYQANK NQSIHNWIRY KEGFSSELVQ NLIEEFGLSK GDTILDPFLG SGTTSLTAKM 130 140 150 160 170 180 LGINSIGIDI LPISHIAFEP KSFIFEYNLE ELDRAYKEIY EISPTKIEQK FNHLSITEGA 190 200 210 220 230 240 FPEETENDLL FFTHWDNNSQ YSYQTKTLIK LILVSILEEI SYTRKDGQYL RWDYRSQKVI 250 260 270 280 290 300 ETNKKRLEQG KEPIKTILDK GELPTVKESL LNTLLTIKED IKEIQQKCLP NESVHELIKD 310 320 330 340 350 360 SALNALPKIN DNTFDAVITS PPYCNRYDYT RTYALELAYL GVDEKKIREL RQAQLSCTVE 370 380 390 400 410 420 NKSKLKQLKD YYHSLFLESR YAEIERLVTG NEVLNEINYA LRKRWENGEV NNKGILSMVD 430 440 450 460 470 480 GYFTELTFIF YELFRTCKPG AKVAFVNDNV RYAGEIIPVD FLSTKIAEDI GFKPIKIYTL 490 500 KQRKGNSSQQ MGKYGRVALR KSITIWEKP

References

[1]

"Cloning and sequence comparison of AvaI and BsoBI restriction-modification systems." Ruan H., Lunnen K.D., Scott M.E., Moran L.S., Slatko B.E., Pelletier J.J., Hess E.J., Benner J. II, Wilson G.G., Xu S.-Y. Mol. Gen. Genet. 252:695-699(1996) [PubMed: 8917312] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: JN209.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X98287 Genomic DNA. Translation: CAA66933.1.
3D structure databases
SMR	P70986. Positions 81-133.
ModBase	Search...
Protein family/group databases
REBASE	3311. M.BsoBI.
Family and domain databases
InterPro	IPR002941. DNA_methylase_N4/N6. IPR017985. MeTrfase_CN4_CS. [Graphical view]
Pfam	PF01555. N6_N4_Mtase. 2 hits. [Graphical view]
PROSITE	PS00093. N4_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTB1_BACST

Accession

Primary (citable) accession number: P70986

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 8, 2000
Last sequence update:	July 1, 1997
Last modified:	January 19, 2010
This is version 55 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)