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Reviewed, UniProtKB/Swiss-Prot P70986 (MTB1_BACST)

Last modified January 19, 2010. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Modification methylase BsoBI
      Short name=M.BsoBI
    EC=2.1.1.113
Alternative name(s):
    N(4)- cytosine-specific methyltransferase BsoBI
Gene names
Name: bsoBIM
OrganismBacillus stearothermophilus (Geobacillus stearothermophilus)
Taxonomic identifier1422 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacillalesBacillaceaeGeobacillus

Protein attributes

Sequence length509 AA.
Sequence statusComplete.
Protein existenceInferred from homology.

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence CYCGRG, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the BsoBI endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA cytosine = S-adenosyl-L-homocysteine + DNA N(4)-methylcytosine.

Sequence similarities

Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 509509Modification methylase BsoBI
PRO_0000087925

Sequences

Sequence LengthMass (Da)Tools
P70986-1 [UniParc].

Last modified July 1, 1997. Version 2.
Checksum: 3C472905C3276D2D

FASTA50959,144
        10         20         30         40         50         60 
METVQMSLDL QPEDTKANIE CSSPDYEIKC FSHNQFAPQI ERLKIEKKYI HIVEETETFN 

        70         80         90        100        110        120 
RKLVSYQANK NQSIHNWIRY KEGFSSELVQ NLIEEFGLSK GDTILDPFLG SGTTSLTAKM 

       130        140        150        160        170        180 
LGINSIGIDI LPISHIAFEP KSFIFEYNLE ELDRAYKEIY EISPTKIEQK FNHLSITEGA 

       190        200        210        220        230        240 
FPEETENDLL FFTHWDNNSQ YSYQTKTLIK LILVSILEEI SYTRKDGQYL RWDYRSQKVI 

       250        260        270        280        290        300 
ETNKKRLEQG KEPIKTILDK GELPTVKESL LNTLLTIKED IKEIQQKCLP NESVHELIKD 

       310        320        330        340        350        360 
SALNALPKIN DNTFDAVITS PPYCNRYDYT RTYALELAYL GVDEKKIREL RQAQLSCTVE 

       370        380        390        400        410        420 
NKSKLKQLKD YYHSLFLESR YAEIERLVTG NEVLNEINYA LRKRWENGEV NNKGILSMVD 

       430        440        450        460        470        480 
GYFTELTFIF YELFRTCKPG AKVAFVNDNV RYAGEIIPVD FLSTKIAEDI GFKPIKIYTL 

       490        500 
KQRKGNSSQQ MGKYGRVALR KSITIWEKP 

« Hide

References

[1]"Cloning and sequence comparison of AvaI and BsoBI restriction-modification systems."
Ruan H., Lunnen K.D., Scott M.E., Moran L.S., Slatko B.E., Pelletier J.J., Hess E.J., Benner J. II, Wilson G.G., Xu S.-Y.
Mol. Gen. Genet. 252:695-699(1996) [PubMed: 8917312] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: JN209.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X98287 Genomic DNA. Translation: CAA66933.1.

3D structure databases

SMRP70986. Positions 81-133.
ModBaseSearch...

Protein family/group databases

REBASE3311. M.BsoBI.

Family and domain databases

InterProIPR002941. DNA_methylase_N4/N6.
IPR017985. MeTrfase_CN4_CS.
[Graphical view]
PfamPF01555. N6_N4_Mtase. 2 hits.
[Graphical view]
PROSITEPS00093. N4_MTASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTB1_BACST
AccessionPrimary (citable) accession number: P70986
Entry history
Integrated into UniProtKB/Swiss-Prot: December 8, 2000
Last sequence update: July 1, 1997
Last modified: January 19, 2010
This is version 55 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents