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P70985

- T2B1_GEOSE

UniProt

P70985 - T2B1_GEOSE

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Protein

Type-2 restriction enzyme BsoBI

Gene

bsoBIR

Organism
Geobacillus stearothermophilus (Bacillus stearothermophilus)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Recognizes the double-stranded sequence CYCGRG and cleaves after C-1.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei81 – 811Interaction with DNA
Sitei131 – 1311Interaction with DNA
Metal bindingi212 – 2121Magnesium; catalyticSequence Analysis
Metal bindingi240 – 2401Magnesium; catalyticSequence Analysis
Metal bindingi242 – 2421Magnesium; catalyticSequence Analysis

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. metal ion binding Source: UniProtKB-KW
  3. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Keywords - Ligandi

Magnesium, Metal-binding

Protein family/group databases

REBASEi392. BsoBI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme BsoBI (EC:3.1.21.4)
Short name:
R.BsoBI
Alternative name(s):
Endonuclease BsoBI
Type II restriction enzyme BsoBI
Gene namesi
Name:bsoBIR
OrganismiGeobacillus stearothermophilus (Bacillus stearothermophilus)
Taxonomic identifieri1422 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 323323Type-2 restriction enzyme BsoBIPRO_0000077288Add
BLAST

Interactioni

Subunit structurei

Homodimer.1 Publication

Structurei

Secondary structure

1
323
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi6 – 94
Helixi14 – 174
Helixi21 – 5333
Helixi59 – 646
Helixi66 – 683
Helixi69 – 757
Helixi80 – 834
Helixi88 – 10114
Turni102 – 1065
Helixi107 – 1093
Helixi110 – 15243
Beta strandi157 – 1615
Beta strandi170 – 1723
Helixi180 – 1834
Beta strandi184 – 1918
Beta strandi194 – 20411
Turni205 – 2084
Beta strandi209 – 21810
Helixi232 – 2343
Beta strandi235 – 2428
Helixi247 – 27024
Beta strandi276 – 2838
Helixi286 – 29712
Beta strandi303 – 3064
Helixi310 – 32112

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DC1X-ray1.70A/B1-323[»]
ProteinModelPortaliP70985.
SMRiP70985. Positions 4-323.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP70985.

Family & Domainsi

Family and domain databases

Gene3Di3.40.91.10. 2 hits.
InterProiIPR011335. Restrct_endonuc-II-like.
IPR015277. Restrct_endonuc_II_AvaI/BsoBI.
IPR011544. Restrct_endonuc_II_Cfr10/BsoB1.
[Graphical view]
PfamiPF09194. Endonuc-BsobI. 1 hit.
[Graphical view]
ProDomiPD040871. Restrict_endonuc_II_AvaI/BsoBI. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF52980. SSF52980. 1 hit.

Sequencei

Sequence statusi: Complete.

P70985-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNTQKPFENH LKSVDDLKTT YEEYRAGFIA FALEKNKRST PYIERARALK
60 70 80 90 100
VAASVAKTPK DLLYLEDIQD ALLYASGISD KAKKFLTEDD KKESINNLIE
110 120 130 140 150
NFLEPAGEEF IDELIFRYLL FQGDSLGGTM RNIAGALAQQ KLTRAIISAL
160 170 180 190 200
DIANIPYKWL DSRDKKYTNW MDKPEDDYEL ETFAKGISWT INGKHRTLMY
210 220 230 240 250
NITVPLVKKN VDICLFNCEP EIYTPQKVHQ QPEKYLLLGE LKGGIDPAGA
260 270 280 290 300
DEHWKTANTA LTRIRNKFSE KGLSPKTIFI GAAIEHSMAE EIWDQLQSGS
310 320
LTNSANLTKT EQVGSLCRWI INI
Length:323
Mass (Da):36,706
Last modified:February 1, 1997 - v1
Checksum:iC5D71CA25DA95B27
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X98287 Genomic DNA. Translation: CAA66932.1.
PIRiS72473.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X98287 Genomic DNA. Translation: CAA66932.1 .
PIRi S72473.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1DC1 X-ray 1.70 A/B 1-323 [» ]
ProteinModelPortali P70985.
SMRi P70985. Positions 4-323.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 392. BsoBI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P70985.

Family and domain databases

Gene3Di 3.40.91.10. 2 hits.
InterProi IPR011335. Restrct_endonuc-II-like.
IPR015277. Restrct_endonuc_II_AvaI/BsoBI.
IPR011544. Restrct_endonuc_II_Cfr10/BsoB1.
[Graphical view ]
Pfami PF09194. Endonuc-BsobI. 1 hit.
[Graphical view ]
ProDomi PD040871. Restrict_endonuc_II_AvaI/BsoBI. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SUPFAMi SSF52980. SSF52980. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Cloning and sequence comparison of AvaI and BsoBI restriction-modification systems."
    Ruan H., Lunnen K.D., Scott M.E., Moran L.S., Slatko B.E., Pelletier J.J., Hess E.J., Benner J. II, Wilson G.G., Xu S.-Y.
    Mol. Gen. Genet. 252:695-699(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: JN209.
  2. "Overexpression of BsoBI restriction endonuclease in E. coli, purification of the recombinant BsoBI, and identification of catalytic residues of BsoBI by random mutagenesis."
    Ruan H., Lunnen K.D., Pelletier J.J., Xu S.-Y.
    Gene 188:35-39(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, MUTAGENESIS.
    Strain: JN209.
  3. "Restriction enzyme BsoBI-DNA complex: a tunnel for recognition of degenerate DNA sequences and potential histidine catalysis."
    van der Woerd M.J., Pelletier J.J., Xu S.-Y., Friedman A.M.
    Structure 9:133-144(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) IN COMPLEX WITH DNA, SUBUNIT.

Entry informationi

Entry nameiT2B1_GEOSE
AccessioniPrimary (citable) accession number: P70985
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 8, 2000
Last sequence update: February 1, 1997
Last modified: October 1, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3