Reviewed, UniProtKB/Swiss-Prot P70985 (T2B1_BACST)

Names and origin

Protein names

Recommended name:
    Type-2 restriction enzyme BsoBI
      Short name=R.BsoBI
    EC=3.1.21.4
Alternative name(s):
    Type II restriction enzyme BsoBI
    Endonuclease BsoBI

Gene names

Name:

bsoBIR

Organism

Bacillus stearothermophilus (Geobacillus stearothermophilus)

Taxonomic identifier

1422 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length	323 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Recognizes the double-stranded sequence CYCGRG and cleaves after C-1.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.
Subunit structure	Homodimer. Ref.3

Ontologies

Keywords
Biological process	Restriction system
Ligand	Magnesium Metal-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	3D-structure
Gene Ontology (GO)
Biological process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 323

323

Type-2 restriction enzyme BsoBI

PRO_0000077288

Sites

Metal binding

212

1

Magnesium; catalytic Potential

Metal binding

240

1

Magnesium; catalytic Potential

Metal binding

242

1

Magnesium; catalytic Potential

Site

81

1

Interaction with DNA

Site

131

1

Interaction with DNA

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

323

Helix Strand Turn

Details...

Helix

6 – 9

4

Helix

14 – 17

4

Helix

21 – 53

33

Helix

59 – 64

6

Helix

66 – 68

3

Helix

69 – 75

7

Helix

80 – 83

4

Helix

88 – 101

14

Turn

102 – 106

5

Helix

107 – 109

3

Helix

110 – 152

43

Beta strand

157 – 161

5

Beta strand

170 – 172

3

Helix

180 – 183

4

Beta strand

184 – 191

8

Beta strand

194 – 204

11

Turn

205 – 208

4

Beta strand

209 – 218

10

Helix

232 – 234

3

Beta strand

235 – 242

8

Helix

247 – 270

24

Beta strand

276 – 283

8

Helix

286 – 297

12

Beta strand

303 – 306

4

Helix

310 – 321

12

Sequences

	Sequence		Length	Mass (Da)
	P70985-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: C5D71CA25DA95B27	FASTA	323	36,706
	10 20 30 40 50 60 MNTQKPFENH LKSVDDLKTT YEEYRAGFIA FALEKNKRST PYIERARALK VAASVAKTPK 70 80 90 100 110 120 DLLYLEDIQD ALLYASGISD KAKKFLTEDD KKESINNLIE NFLEPAGEEF IDELIFRYLL 130 140 150 160 170 180 FQGDSLGGTM RNIAGALAQQ KLTRAIISAL DIANIPYKWL DSRDKKYTNW MDKPEDDYEL 190 200 210 220 230 240 ETFAKGISWT INGKHRTLMY NITVPLVKKN VDICLFNCEP EIYTPQKVHQ QPEKYLLLGE 250 260 270 280 290 300 LKGGIDPAGA DEHWKTANTA LTRIRNKFSE KGLSPKTIFI GAAIEHSMAE EIWDQLQSGS 310 320 LTNSANLTKT EQVGSLCRWI INI

References

[1]	"Cloning and sequence comparison of AvaI and BsoBI restriction-modification systems." Ruan H., Lunnen K.D., Scott M.E., Moran L.S., Slatko B.E., Pelletier J.J., Hess E.J., Benner J. II, Wilson G.G., Xu S.-Y. Mol. Gen. Genet. 252:695-699(1996) [PubMed: 8917312] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: JN209.
[2]	"Overexpression of BsoBI restriction endonuclease in E. coli, purification of the recombinant BsoBI, and identification of catalytic residues of BsoBI by random mutagenesis." Ruan H., Lunnen K.D., Pelletier J.J., Xu S.-Y. Gene 188:35-39(1997) [PubMed: 9099856] [Abstract] Cited for: CHARACTERIZATION, MUTAGENESIS. Strain: JN209.
[3]	"Restriction enzyme BsoBI-DNA complex: a tunnel for recognition of degenerate DNA sequences and potential histidine catalysis." van der Woerd M.J., Pelletier J.J., Xu S.-Y., Friedman A.M. Structure 9:133-144(2001) [PubMed: 11250198] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) IN COMPLEX WITH DNA, SUBUNIT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X98287 Genomic DNA. Translation: CAA66932.1.

PIR

S72473.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1DC1	X-ray	1.70	A/B	1-323	[»]

ModBase

Search...

Protein family/group databases

REBASE

392. BsoBI.

Enzyme and pathway databases

BRENDA

3.1.21.4. 266715.

Family and domain databases

InterPro

IPR011544. Restrct_endonuc_II_Cfr10/BsoB1.
IPR011335. Restrict_endonuc_II-like_core.
IPR015277. Restrict_endonuc_II_AvaI/BsoBI.
[Graphical view]

Gene3D

G3DSA:3.40.91.10. Restrct_endonuc_II_Cfr10/BsoB1. 1 hit.

Pfam

PF09194. Endonuc-BsobI. 1 hit.
[Graphical view]

ProDom

PD040871. Restrict_endonuc_II_AvaI/BsoBI. 1 hit.
[Graphical view] [Entries sharing at least one domain]

ProtoNet

Search...

Entry information

Entry name

T2B1_BACST

Accession

Primary (citable) accession number: P70985

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 8, 2000
Last sequence update:	February 1, 1997
Last modified:	November 24, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)