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P70985

- T2B1_GEOSE

UniProt

P70985 - T2B1_GEOSE

Protein

Type-2 restriction enzyme BsoBI

Gene

bsoBIR

Organism
Geobacillus stearothermophilus (Bacillus stearothermophilus)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
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    Functioni

    Recognizes the double-stranded sequence CYCGRG and cleaves after C-1.

    Catalytic activityi

    Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei81 – 811Interaction with DNA
    Sitei131 – 1311Interaction with DNA
    Metal bindingi212 – 2121Magnesium; catalyticSequence Analysis
    Metal bindingi240 – 2401Magnesium; catalyticSequence Analysis
    Metal bindingi242 – 2421Magnesium; catalyticSequence Analysis

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. metal ion binding Source: UniProtKB-KW
    3. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    Magnesium, Metal-binding

    Protein family/group databases

    REBASEi392. BsoBI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-2 restriction enzyme BsoBI (EC:3.1.21.4)
    Short name:
    R.BsoBI
    Alternative name(s):
    Endonuclease BsoBI
    Type II restriction enzyme BsoBI
    Gene namesi
    Name:bsoBIR
    OrganismiGeobacillus stearothermophilus (Bacillus stearothermophilus)
    Taxonomic identifieri1422 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 323323Type-2 restriction enzyme BsoBIPRO_0000077288Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Structurei

    Secondary structure

    1
    323
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi6 – 94
    Helixi14 – 174
    Helixi21 – 5333
    Helixi59 – 646
    Helixi66 – 683
    Helixi69 – 757
    Helixi80 – 834
    Helixi88 – 10114
    Turni102 – 1065
    Helixi107 – 1093
    Helixi110 – 15243
    Beta strandi157 – 1615
    Beta strandi170 – 1723
    Helixi180 – 1834
    Beta strandi184 – 1918
    Beta strandi194 – 20411
    Turni205 – 2084
    Beta strandi209 – 21810
    Helixi232 – 2343
    Beta strandi235 – 2428
    Helixi247 – 27024
    Beta strandi276 – 2838
    Helixi286 – 29712
    Beta strandi303 – 3064
    Helixi310 – 32112

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1DC1X-ray1.70A/B1-323[»]
    ProteinModelPortaliP70985.
    SMRiP70985. Positions 4-323.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP70985.

    Family & Domainsi

    Family and domain databases

    Gene3Di3.40.91.10. 2 hits.
    InterProiIPR011335. Restrct_endonuc-II-like.
    IPR015277. Restrct_endonuc_II_AvaI/BsoBI.
    IPR011544. Restrct_endonuc_II_Cfr10/BsoB1.
    [Graphical view]
    PfamiPF09194. Endonuc-BsobI. 1 hit.
    [Graphical view]
    ProDomiPD040871. Restrict_endonuc_II_AvaI/BsoBI. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF52980. SSF52980. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P70985-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNTQKPFENH LKSVDDLKTT YEEYRAGFIA FALEKNKRST PYIERARALK    50
    VAASVAKTPK DLLYLEDIQD ALLYASGISD KAKKFLTEDD KKESINNLIE 100
    NFLEPAGEEF IDELIFRYLL FQGDSLGGTM RNIAGALAQQ KLTRAIISAL 150
    DIANIPYKWL DSRDKKYTNW MDKPEDDYEL ETFAKGISWT INGKHRTLMY 200
    NITVPLVKKN VDICLFNCEP EIYTPQKVHQ QPEKYLLLGE LKGGIDPAGA 250
    DEHWKTANTA LTRIRNKFSE KGLSPKTIFI GAAIEHSMAE EIWDQLQSGS 300
    LTNSANLTKT EQVGSLCRWI INI 323
    Length:323
    Mass (Da):36,706
    Last modified:February 1, 1997 - v1
    Checksum:iC5D71CA25DA95B27
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X98287 Genomic DNA. Translation: CAA66932.1.
    PIRiS72473.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X98287 Genomic DNA. Translation: CAA66932.1 .
    PIRi S72473.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1DC1 X-ray 1.70 A/B 1-323 [» ]
    ProteinModelPortali P70985.
    SMRi P70985. Positions 4-323.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 392. BsoBI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P70985.

    Family and domain databases

    Gene3Di 3.40.91.10. 2 hits.
    InterProi IPR011335. Restrct_endonuc-II-like.
    IPR015277. Restrct_endonuc_II_AvaI/BsoBI.
    IPR011544. Restrct_endonuc_II_Cfr10/BsoB1.
    [Graphical view ]
    Pfami PF09194. Endonuc-BsobI. 1 hit.
    [Graphical view ]
    ProDomi PD040871. Restrict_endonuc_II_AvaI/BsoBI. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SUPFAMi SSF52980. SSF52980. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequence comparison of AvaI and BsoBI restriction-modification systems."
      Ruan H., Lunnen K.D., Scott M.E., Moran L.S., Slatko B.E., Pelletier J.J., Hess E.J., Benner J. II, Wilson G.G., Xu S.-Y.
      Mol. Gen. Genet. 252:695-699(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: JN209.
    2. "Overexpression of BsoBI restriction endonuclease in E. coli, purification of the recombinant BsoBI, and identification of catalytic residues of BsoBI by random mutagenesis."
      Ruan H., Lunnen K.D., Pelletier J.J., Xu S.-Y.
      Gene 188:35-39(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION, MUTAGENESIS.
      Strain: JN209.
    3. "Restriction enzyme BsoBI-DNA complex: a tunnel for recognition of degenerate DNA sequences and potential histidine catalysis."
      van der Woerd M.J., Pelletier J.J., Xu S.-Y., Friedman A.M.
      Structure 9:133-144(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) IN COMPLEX WITH DNA, SUBUNIT.

    Entry informationi

    Entry nameiT2B1_GEOSE
    AccessioniPrimary (citable) accession number: P70985
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 8, 2000
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries

    External Data

    Dasty 3