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P70985 (T2B1_GEOSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme BsoBI

Short name=R.BsoBI
EC=3.1.21.4
Alternative name(s):
Endonuclease BsoBI
Type II restriction enzyme BsoBI
Gene names
Name:bsoBIR
OrganismGeobacillus stearothermophilus (Bacillus stearothermophilus)
Taxonomic identifier1422 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

Protein attributes

Sequence length323 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Recognizes the double-stranded sequence CYCGRG and cleaves after C-1.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Subunit structure

Homodimer. Ref.3

Ontologies

Keywords
   Biological processRestriction system
   LigandMagnesium
Metal-binding
   Molecular functionEndonuclease
Hydrolase
Nuclease
   Technical term3D-structure
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: InterPro

Type II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 323323Type-2 restriction enzyme BsoBI
PRO_0000077288

Sites

Metal binding2121Magnesium; catalytic Potential
Metal binding2401Magnesium; catalytic Potential
Metal binding2421Magnesium; catalytic Potential
Site811Interaction with DNA
Site1311Interaction with DNA

Secondary structure

........................................... 323
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P70985 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: C5D71CA25DA95B27

FASTA32336,706
        10         20         30         40         50         60 
MNTQKPFENH LKSVDDLKTT YEEYRAGFIA FALEKNKRST PYIERARALK VAASVAKTPK 

        70         80         90        100        110        120 
DLLYLEDIQD ALLYASGISD KAKKFLTEDD KKESINNLIE NFLEPAGEEF IDELIFRYLL 

       130        140        150        160        170        180 
FQGDSLGGTM RNIAGALAQQ KLTRAIISAL DIANIPYKWL DSRDKKYTNW MDKPEDDYEL 

       190        200        210        220        230        240 
ETFAKGISWT INGKHRTLMY NITVPLVKKN VDICLFNCEP EIYTPQKVHQ QPEKYLLLGE 

       250        260        270        280        290        300 
LKGGIDPAGA DEHWKTANTA LTRIRNKFSE KGLSPKTIFI GAAIEHSMAE EIWDQLQSGS 

       310        320 
LTNSANLTKT EQVGSLCRWI INI 

« Hide

References

[1]"Cloning and sequence comparison of AvaI and BsoBI restriction-modification systems."
Ruan H., Lunnen K.D., Scott M.E., Moran L.S., Slatko B.E., Pelletier J.J., Hess E.J., Benner J. II, Wilson G.G., Xu S.-Y.
Mol. Gen. Genet. 252:695-699(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: JN209.
[2]"Overexpression of BsoBI restriction endonuclease in E. coli, purification of the recombinant BsoBI, and identification of catalytic residues of BsoBI by random mutagenesis."
Ruan H., Lunnen K.D., Pelletier J.J., Xu S.-Y.
Gene 188:35-39(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION, MUTAGENESIS.
Strain: JN209.
[3]"Restriction enzyme BsoBI-DNA complex: a tunnel for recognition of degenerate DNA sequences and potential histidine catalysis."
van der Woerd M.J., Pelletier J.J., Xu S.-Y., Friedman A.M.
Structure 9:133-144(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) IN COMPLEX WITH DNA, SUBUNIT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X98287 Genomic DNA. Translation: CAA66932.1.
PIRS72473.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1DC1X-ray1.70A/B1-323[»]
ProteinModelPortalP70985.
SMRP70985. Positions 4-323.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE392. BsoBI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.91.10. 2 hits.
InterProIPR011335. Restrct_endonuc-II-like.
IPR015277. Restrct_endonuc_II_AvaI/BsoBI.
IPR011544. Restrct_endonuc_II_Cfr10/BsoB1.
[Graphical view]
PfamPF09194. Endonuc-BsobI. 1 hit.
[Graphical view]
ProDomPD040871. Restrict_endonuc_II_AvaI/BsoBI. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMSSF52980. SSF52980. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP70985.

Entry information

Entry nameT2B1_GEOSE
AccessionPrimary (citable) accession number: P70985
Entry history
Integrated into UniProtKB/Swiss-Prot: December 8, 2000
Last sequence update: February 1, 1997
Last modified: April 16, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references