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Protein

Type-2 restriction enzyme BsoBI

Gene

bsoBIR

Organism
Geobacillus stearothermophilus (Bacillus stearothermophilus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Recognizes the double-stranded sequence CYCGRG and cleaves after C-1.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi212Magnesium; catalyticSequence analysis1
Metal bindingi240Magnesium; catalyticSequence analysis1
Metal bindingi242Magnesium; catalyticSequence analysis1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease
Biological processRestriction system
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme BsoBI (EC:3.1.21.4)
Short name:
R.BsoBI
Alternative name(s):
Endonuclease BsoBI
Type II restriction enzyme BsoBI
Gene namesi
Name:bsoBIR
OrganismiGeobacillus stearothermophilus (Bacillus stearothermophilus)
Taxonomic identifieri1422 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000772881 – 323Type-2 restriction enzyme BsoBIAdd BLAST323

Interactioni

Subunit structurei

Homodimer.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei81Interaction with DNA1
Sitei131Interaction with DNA1

Structurei

Secondary structure

1323
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 9Combined sources4
Helixi14 – 17Combined sources4
Helixi21 – 53Combined sources33
Helixi59 – 64Combined sources6
Helixi66 – 68Combined sources3
Helixi69 – 75Combined sources7
Helixi80 – 83Combined sources4
Helixi88 – 101Combined sources14
Turni102 – 106Combined sources5
Helixi107 – 109Combined sources3
Helixi110 – 152Combined sources43
Beta strandi157 – 161Combined sources5
Beta strandi170 – 172Combined sources3
Helixi180 – 183Combined sources4
Beta strandi184 – 191Combined sources8
Beta strandi194 – 204Combined sources11
Turni205 – 208Combined sources4
Beta strandi209 – 218Combined sources10
Helixi232 – 234Combined sources3
Beta strandi235 – 242Combined sources8
Helixi247 – 270Combined sources24
Beta strandi276 – 283Combined sources8
Helixi286 – 297Combined sources12
Beta strandi303 – 306Combined sources4
Helixi310 – 321Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DC1X-ray1.70A/B1-323[»]
ProteinModelPortaliP70985.
SMRiP70985.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP70985.

Family & Domainsi

Family and domain databases

InterProiView protein in InterPro
IPR011335. Restrct_endonuc-II-like.
IPR015277. Restrct_endonuc_II_AvaI/BsoBI.
PfamiView protein in Pfam
PF09194. Endonuc-BsobI. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD040871. Restrict_endonuc_II_AvaI/BsoBI. 1 hit.
SUPFAMiSSF52980. SSF52980. 1 hit.

Sequencei

Sequence statusi: Complete.

P70985-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTQKPFENH LKSVDDLKTT YEEYRAGFIA FALEKNKRST PYIERARALK
60 70 80 90 100
VAASVAKTPK DLLYLEDIQD ALLYASGISD KAKKFLTEDD KKESINNLIE
110 120 130 140 150
NFLEPAGEEF IDELIFRYLL FQGDSLGGTM RNIAGALAQQ KLTRAIISAL
160 170 180 190 200
DIANIPYKWL DSRDKKYTNW MDKPEDDYEL ETFAKGISWT INGKHRTLMY
210 220 230 240 250
NITVPLVKKN VDICLFNCEP EIYTPQKVHQ QPEKYLLLGE LKGGIDPAGA
260 270 280 290 300
DEHWKTANTA LTRIRNKFSE KGLSPKTIFI GAAIEHSMAE EIWDQLQSGS
310 320
LTNSANLTKT EQVGSLCRWI INI
Length:323
Mass (Da):36,706
Last modified:February 1, 1997 - v1
Checksum:iC5D71CA25DA95B27
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98287 Genomic DNA. Translation: CAA66932.1.
PIRiS72473.

Similar proteinsi

Entry informationi

Entry nameiT2B1_GEOSE
AccessioniPrimary (citable) accession number: P70985
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 8, 2000
Last sequence update: February 1, 1997
Last modified: September 27, 2017
This is version 85 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries