Unreviewed, UniProtKB/TrEMBL P70983 (P70983_BACSP)

Names and origin

Protein names	Submitted name:     Alkaline amylopullulanase EMBL BAA11332.1
Organism	Bacillus sp. EMBL BAA11332.1
Taxonomic identifier	1409 [NCBI]
Taxonomic lineage	Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	1938 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Predicted.

Ontologies

Keywords
Cellular component	Cell wall RuleBase RU000373V1 Secreted
PTM	Peptidoglycan-anchor RuleBase RU000373V1
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cell surface Inferred from electronic annotation. Source: InterPro
Molecular function	carbohydrate binding Inferred from electronic annotation. Source: InterPro cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	P70983-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: A29D3F715EB6A72E	FASTA	1,938	214,918
	10 20 30 40 50 60 MKKRFQRGMA GLLSILLIVS MFAGYLPARA AAETGDKRIE FSYERPDGNY EGWNLWVWGT 70 80 90 100 110 120 GVKDDQIDFT EFKEGKAYAD IAVSDNADKV GFIIRKGDWE EKDFDGDRSI TINKIDNITK 130 140 150 160 170 180 VHVTSQQEKF GQIPDGSPPV VADGNADFFF RDKELYAAGE MDKVEKVELS ILGEKYEMNG 190 200 210 220 230 240 EPEKERFTYT LSDLPTGEHE YTYLVTVDGQ TEEVTDPYNT VDGRSVVEYV TSDVQVSASF 250 260 270 280 290 300 IPAKVDYNQN AVVKVDIESE TETKIREMSI NLSEIGGKEK ATIDPALNEL TVAVKQGVTA 310 320 330 340 350 360 GVKNLPITAI DEFGNRHEGS ATLEVQARTI TGEKADFDWD QSVVYFMLTD RFFDGDSSNN 370 380 390 400 410 420 DPHGIGYDTS KSGTYQGGDF KGITQRLDYL DELGINTIWI SPVVDNIKFD VRHSEGPDTP 430 440 450 460 470 480 YYAYHGYWAD NFGELNPHFG SMADFHEMID AAHERGIKIM VDVVLNHTGY GLKPGDSSSV 490 500 510 520 530 540 ANFPTDEDRA RFDGMLRDGG SGEVRGELAG LPDFLTENPD VREQVVQWQT DWIEKSRTAK 550 560 570 580 590 600 GNTIDYFRVD TVKHVEDTTW MAFKNALTKA MPEHKLIGEA WGANVNDDLG YLNSGMMDSL 610 620 630 640 650 660 LDFDFKNYAR DFANGQLDAV QQKLEARNSK LNNTATLGQF LGSHDEDRFY EVVEGDLGKY 670 680 690 700 710 720 QVAASLQLTA KGQPVIYYGE ELGLPGKNDY PYYTNRQNMP WDDVDGNEIL EHYQKLLAFR 730 740 750 760 770 780 NDNPNTFAKG DRKKVAGSDS EGYLLFSRTY GENSVYVGLN TEAAAKDVTL NFGSSEAVVT 790 800 810 820 830 840 DRYSGQEYQA NEEGQVTFSI PAMEDGGTVL LEVENGAVPP VEEEPTEPGE IEENTLRIHY 850 860 870 880 890 900 QRTDNSYENL GLWLWGDVAA PSENWPSGGT PFQAGNVTDY GAYVDVELAE DAQNIGFLVL 910 920 930 940 950 960 NTTNGDKDGG DKAVELFSPD LNEIWIKQGS DEVFLYEPVD LPANTVRIHY ERTNADYEGW 970 980 990 1000 1010 1020 GLWNWEDVES PSDGWPNGAA DAAGIGKYGA YYDIKLKEDA NKIGFLFVNK QSGGQTGDMT 1030 1040 1050 1060 1070 1080 FDMLKQYNQL FVKEGEDKVY TNPYGTVPLA LVSGEVLSDK LISLTFTRTE GLDLEELKEQ 1090 1100 1110 1120 1130 1140 LEIKDVDGND VSFTDVTIEG EKTVHVHGEF DLEKIPFSVT YLDRTISVKS GWKLIDEMYA 1150 1160 1170 1180 1190 1200 YDGKLGAELH EDGTATLKVW SPKADNVSVV LYDKVDQNEV VDTIEMVKGD RGVWSVKLTK 1210 1220 1230 1240 1250 1260 DNTGLDSLKG YYYHYEITHG DVTNLALDPY AKSMAAWNNE AGDKVGKAAI VDIGSIGPEL 1270 1280 1290 1300 1310 1320 DYADIPGFEK REDTIIYEVH VRDFTSDPNI GEDLKAQFGT FASFVEKLDY IQELGVTHIQ 1330 1340 1350 1360 1370 1380 LLPVMSYYFS NEFESGERML EYASTGTNYN WGYDPHNYFS LSGMYSENPE DPELRIKEFK 1390 1400 1410 1420 1430 1440 NLINEIHKRD MGVVLDVVFN HTAQVHIFED LVPNYYHFMD ADGTPRTSFG GGRLGTTHEM 1450 1460 1470 1480 1490 1500 SRRVLVDSIK HWVDEYKVDG FRFDMMGDHD AESIQIAFDE AKKLNPNIVM IGEGWVTFAG 1510 1520 1530 1540 1550 1560 DEGEPVQAAD QQWMQYTEAV GSFSDEFRNE LKSGFGSEGQ PRFITGGAVN VQQIFDNIKA 1570 1580 1590 1600 1610 1620 QPHNFMADQP GDVVQYIEAH DNLTLYDVIA QSIKKDPEIA ENDLEIHKRI RVGNAMVLTS 1630 1640 1650 1660 1670 1680 QGTAFLHAGQ EFGRTKQWRA PATEAPYKST YMTDADGNPF VYPYFIHDSY DSSDIINRFD 1690 1700 1710 1720 1730 1740 WEKATDAEKY PVNNVTRDYT AGLIELRRSS DAFRLGSREL VDSNVTMVDA PEIKEQDLVV 1750 1760 1770 1780 1790 1800 AYRSVSTAGV EYYTFVNADT SSRTLTLGQD LTEGVVVVDA EEANVAGVAE PAGFELTAEG 1810 1820 1830 1840 1850 1860 ITLEPLTTVV VRVGEQEGTD PGDGDGDGNT PPPGDGDGDG NTPPPGDGDG DGNTPPPGNG 1870 1880 1890 1900 1910 1920 NGNNPGTPPG KGGENPGKGK NDKTPPGKGG DNPGKGNKLP LTATGTLNYI LFGAIMLVLG 1930 TLLYLGVRRK AGLKEKTL

References

[1]

"Amino acid sequence and molecular structure of an alkaline amylopullulanase from Bacillus that hydrolyzes alpha-1,4 and alpha-1,6 linkages in polysaccharides at different active sites." Hatada Y., Igarashi K., Ozaki K., Ara K., Hitomi J., Kobayashi T., Kawai S., Watabe T., Ito S. J. Biol. Chem. 271:24075-24083(1996) [PubMed: 8798645] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: KSM-1378 EMBL BAA11332.1.

[2]

"Purification and characterization of an alkaline amylopullulanase with both alpha-1,4 and alpha-1,6 hydrolytic activity from alkalophilic Bacillus sp. KSM-1378." Ara K., Saeki K., Igarashi K., Takaiwa M., Uemura T., Hagihara H., Kawai S., Ito S. Biochim. Biophys. Acta 1243:315-324(1995) [PubMed: 7727505] [Abstract] Cited for: PROTEIN SEQUENCE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D78258 Genomic DNA. Translation: BAA11332.1.
PIR	S60353.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	CBM41. Carbohydrate-Binding Module Family 41. CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Family and domain databases
InterPro	IPR006047. Glyco_hydro_13_cat. IPR004193. Glyco_hydro_13_N. IPR006589. Glyco_hydro_13_sub_cat. IPR013781. Glyco_hydro_sg_catalytic. IPR019948. Gram-positive_anchor. IPR013783. Ig-like_fold. IPR019931. LPXTG-motif_cell_wall_anchor. IPR005323. PUD. IPR011838. Pullulan_Gpos. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 2 hits. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
Pfam	PF00128. Alpha-amylase. 2 hits. PF02922. CBM_48. 1 hit. PF00746. Gram_pos_anchor. 1 hit. PF03714. PUD. 3 hits. [Graphical view]
SMART	SM00642. Aamy. 1 hit. [Graphical view]
TIGRFAMs	TIGR01167. LPXTG_anchor. 1 hit. TIGR02102. pullulan_Gpos. 1 hit.
ProtoNet	Search...

Entry information

Entry name

P70983_BACSP

Accession

Primary (citable) accession number: P70983

Entry history

Integrated into UniProtKB/TrEMBL:	February 1, 1997
Last sequence update:	February 1, 1997
Last modified:	June 16, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)