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Protein
Submitted name:

Alkaline amylopullulanase

Gene
N/A
Organism
Bacillus sp.
Status
Unreviewed-Annotation score: Annotation score: 1 out of 5-Protein inferred from homologyi

Functioni

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Protein family/group databases

CAZyiCBM41. Carbohydrate-Binding Module Family 41.
CBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Submitted name:
Alkaline amylopullulanaseImported
OrganismiBacillus sp.Imported
Taxonomic identifieri1409 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei1907 – 192721HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3030Sequence analysisAdd
BLAST
Chaini31 – 19381908Sequence analysisPRO_5004161398Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP70983.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini346 – 720375AamyInterPro annotationAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.SAAS annotation

Keywords - Domaini

SignalSequence analysis, Transmembrane, Transmembrane helixSequence analysis

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR013784. Carb-bd-like_fold.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR019948. Gram-positive_anchor.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR005323. PUD.
IPR011838. Pullulan_Gpos.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 3 hits.
PfamiPF00128. Alpha-amylase. 3 hits.
PF02922. CBM_48. 1 hit.
PF00746. Gram_pos_anchor. 1 hit.
PF03714. PUD. 3 hits.
[Graphical view]
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF49452. SSF49452. 3 hits.
SSF51445. SSF51445. 3 hits.
SSF81296. SSF81296. 1 hit.
TIGRFAMsiTIGR02102. pullulan_Gpos. 1 hit.

Sequencei

Sequence statusi: Complete.

P70983-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKRFQRGMA GLLSILLIVS MFAGYLPARA AAETGDKRIE FSYERPDGNY
60 70 80 90 100
EGWNLWVWGT GVKDDQIDFT EFKEGKAYAD IAVSDNADKV GFIIRKGDWE
110 120 130 140 150
EKDFDGDRSI TINKIDNITK VHVTSQQEKF GQIPDGSPPV VADGNADFFF
160 170 180 190 200
RDKELYAAGE MDKVEKVELS ILGEKYEMNG EPEKERFTYT LSDLPTGEHE
210 220 230 240 250
YTYLVTVDGQ TEEVTDPYNT VDGRSVVEYV TSDVQVSASF IPAKVDYNQN
260 270 280 290 300
AVVKVDIESE TETKIREMSI NLSEIGGKEK ATIDPALNEL TVAVKQGVTA
310 320 330 340 350
GVKNLPITAI DEFGNRHEGS ATLEVQARTI TGEKADFDWD QSVVYFMLTD
360 370 380 390 400
RFFDGDSSNN DPHGIGYDTS KSGTYQGGDF KGITQRLDYL DELGINTIWI
410 420 430 440 450
SPVVDNIKFD VRHSEGPDTP YYAYHGYWAD NFGELNPHFG SMADFHEMID
460 470 480 490 500
AAHERGIKIM VDVVLNHTGY GLKPGDSSSV ANFPTDEDRA RFDGMLRDGG
510 520 530 540 550
SGEVRGELAG LPDFLTENPD VREQVVQWQT DWIEKSRTAK GNTIDYFRVD
560 570 580 590 600
TVKHVEDTTW MAFKNALTKA MPEHKLIGEA WGANVNDDLG YLNSGMMDSL
610 620 630 640 650
LDFDFKNYAR DFANGQLDAV QQKLEARNSK LNNTATLGQF LGSHDEDRFY
660 670 680 690 700
EVVEGDLGKY QVAASLQLTA KGQPVIYYGE ELGLPGKNDY PYYTNRQNMP
710 720 730 740 750
WDDVDGNEIL EHYQKLLAFR NDNPNTFAKG DRKKVAGSDS EGYLLFSRTY
760 770 780 790 800
GENSVYVGLN TEAAAKDVTL NFGSSEAVVT DRYSGQEYQA NEEGQVTFSI
810 820 830 840 850
PAMEDGGTVL LEVENGAVPP VEEEPTEPGE IEENTLRIHY QRTDNSYENL
860 870 880 890 900
GLWLWGDVAA PSENWPSGGT PFQAGNVTDY GAYVDVELAE DAQNIGFLVL
910 920 930 940 950
NTTNGDKDGG DKAVELFSPD LNEIWIKQGS DEVFLYEPVD LPANTVRIHY
960 970 980 990 1000
ERTNADYEGW GLWNWEDVES PSDGWPNGAA DAAGIGKYGA YYDIKLKEDA
1010 1020 1030 1040 1050
NKIGFLFVNK QSGGQTGDMT FDMLKQYNQL FVKEGEDKVY TNPYGTVPLA
1060 1070 1080 1090 1100
LVSGEVLSDK LISLTFTRTE GLDLEELKEQ LEIKDVDGND VSFTDVTIEG
1110 1120 1130 1140 1150
EKTVHVHGEF DLEKIPFSVT YLDRTISVKS GWKLIDEMYA YDGKLGAELH
1160 1170 1180 1190 1200
EDGTATLKVW SPKADNVSVV LYDKVDQNEV VDTIEMVKGD RGVWSVKLTK
1210 1220 1230 1240 1250
DNTGLDSLKG YYYHYEITHG DVTNLALDPY AKSMAAWNNE AGDKVGKAAI
1260 1270 1280 1290 1300
VDIGSIGPEL DYADIPGFEK REDTIIYEVH VRDFTSDPNI GEDLKAQFGT
1310 1320 1330 1340 1350
FASFVEKLDY IQELGVTHIQ LLPVMSYYFS NEFESGERML EYASTGTNYN
1360 1370 1380 1390 1400
WGYDPHNYFS LSGMYSENPE DPELRIKEFK NLINEIHKRD MGVVLDVVFN
1410 1420 1430 1440 1450
HTAQVHIFED LVPNYYHFMD ADGTPRTSFG GGRLGTTHEM SRRVLVDSIK
1460 1470 1480 1490 1500
HWVDEYKVDG FRFDMMGDHD AESIQIAFDE AKKLNPNIVM IGEGWVTFAG
1510 1520 1530 1540 1550
DEGEPVQAAD QQWMQYTEAV GSFSDEFRNE LKSGFGSEGQ PRFITGGAVN
1560 1570 1580 1590 1600
VQQIFDNIKA QPHNFMADQP GDVVQYIEAH DNLTLYDVIA QSIKKDPEIA
1610 1620 1630 1640 1650
ENDLEIHKRI RVGNAMVLTS QGTAFLHAGQ EFGRTKQWRA PATEAPYKST
1660 1670 1680 1690 1700
YMTDADGNPF VYPYFIHDSY DSSDIINRFD WEKATDAEKY PVNNVTRDYT
1710 1720 1730 1740 1750
AGLIELRRSS DAFRLGSREL VDSNVTMVDA PEIKEQDLVV AYRSVSTAGV
1760 1770 1780 1790 1800
EYYTFVNADT SSRTLTLGQD LTEGVVVVDA EEANVAGVAE PAGFELTAEG
1810 1820 1830 1840 1850
ITLEPLTTVV VRVGEQEGTD PGDGDGDGNT PPPGDGDGDG NTPPPGDGDG
1860 1870 1880 1890 1900
DGNTPPPGNG NGNNPGTPPG KGGENPGKGK NDKTPPGKGG DNPGKGNKLP
1910 1920 1930
LTATGTLNYI LFGAIMLVLG TLLYLGVRRK AGLKEKTL
Length:1,938
Mass (Da):214,918
Last modified:February 1, 1997 - v1
Checksum:iA29D3F715EB6A72E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D78258 Genomic DNA. Translation: BAA11332.1.
PIRiS60353.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D78258 Genomic DNA. Translation: BAA11332.1.
PIRiS60353.

3D structure databases

ProteinModelPortaliP70983.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiCBM41. Carbohydrate-Binding Module Family 41.
CBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR013784. Carb-bd-like_fold.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR019948. Gram-positive_anchor.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR005323. PUD.
IPR011838. Pullulan_Gpos.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 3 hits.
PfamiPF00128. Alpha-amylase. 3 hits.
PF02922. CBM_48. 1 hit.
PF00746. Gram_pos_anchor. 1 hit.
PF03714. PUD. 3 hits.
[Graphical view]
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF49452. SSF49452. 3 hits.
SSF51445. SSF51445. 3 hits.
SSF81296. SSF81296. 1 hit.
TIGRFAMsiTIGR02102. pullulan_Gpos. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Purification and characterization of an alkaline amylopullulanase with both alpha-1,4 and alpha-1,6 hydrolytic activity from alkalophilic Bacillus sp. KSM-1378."
    Ara K., Saeki K., Igarashi K., Takaiwa M., Uemura T., Hagihara H., Kawai S., Ito S.
    Biochim. Biophys. Acta 1243:315-324(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE.
  2. "Amino acid sequence and molecular structure of an alkaline amylopullulanase from Bacillus that hydrolyzes alpha-1,4 and alpha-1,6 linkages in polysaccharides at different active sites."
    Hatada Y., Igarashi K., Ozaki K., Ara K., Hitomi J., Kobayashi T., Kawai S., Watabe T., Ito S.
    J. Biol. Chem. 271:24075-24083(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: KSM-1378Imported.

Entry informationi

Entry nameiP70983_BACSP
AccessioniPrimary (citable) accession number: P70983
Entry historyi
Integrated into UniProtKB/TrEMBL: February 1, 1997
Last sequence update: February 1, 1997
Last modified: July 6, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.