Reviewed, UniProtKB/Swiss-Prot P70920 (ACON_BRAJA)

Names and origin

Protein names

Recommended name:
    Aconitate hydratase
      Short name=Aconitase
    EC=4.2.1.3
Alternative name(s):
    Citrate hydro-lyase

Gene names

Name:	acnA
Ordered Locus Names:	bll0466

Organism

Bradyrhizobium japonicum [Complete proteome] [HAMAP]

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	906 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Citrate = isocitrate.
Cofactor	Binds 1 4Fe-4S cluster per subunit By similarity.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the aconitase/IPM isomerase family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW aconitate hydratase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 906	906	Aconitate hydratase		PRO_0000076656
Sites
	Metal binding	443	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	509	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	512	1	Iron-sulfur (4Fe-4S) By similarity
Experimental info
	Sequence conflict	596	1	W → C in AAC44562. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P70920-1 [UniParc]. Last modified February 28, 2003. Version 2. Checksum: E1A9C4AFA0614104	FASTA	906	98,322
	10 20 30 40 50 60 MTSLDSFKCK KTLKVGAKTY VYYSLPTAEK NGLKGISKLP YSMKVLLENL LRNEDGRSVK 70 80 90 100 110 120 KADIVAVSKW LRKKSLEHEI AFRPARVLMQ DFTGVPAVVD LAAMRNAMQK LGGDAEKINP 130 140 150 160 170 180 LVPVDLVIDH SVIVNFFGDN KAFAKNVTEE YKQNQERYEF LKWGQAAFSN FSVVPPGTGI 190 200 210 220 230 240 CHQVNLEYLS QTVWTKKEKM TVGKKTGTFE VAYPDSLVGT DSHTTMVNGL AVLGWGVGGI 250 260 270 280 290 300 EAEACMLGQP LSMLLPNVVG FKLKGAMKEG VTATDLVLTV TQMLRKLGVV GKFVEFFGPG 310 320 330 340 350 360 LDHLSVADKA TIANMAPEYG ATCGFFPVDA AAIDYLKTSG RAAPRVALVQ AYAKAQGLFR 370 380 390 400 410 420 TAKSADPVFT ETLTLDLADV VPSMAGPKRP EGRIALPSVA EGFSVALANE YKKTEEPAKR 430 440 450 460 470 480 FAVEGKKYEI GHGDVVIAAI TSCTNTSNPS VLIGAGLLAR NAAAKGLKAK PWVKTSLAPG 490 500 510 520 530 540 SQVVAAYLAD SGLQAHLDKV GFNLVGFGCT TCIGNSGPLP EEISKSINDN GIVAAAVLSG 550 560 570 580 590 600 NRNFEGRVSP DVQANYLASP PLVVAHALAG SVTKNLAVEP LGEGKDGKPV YLKDIWPTSK 610 620 630 640 650 660 EINAFMKKFV TASIFKKKYA DVFKGDTNWR KIKTVESETY RWNMSSTYVQ NPPYFEGMKK 670 680 690 700 710 720 EPEPVTDIVE ARILAMFGDK ITTDHISPAG SIKLTSPAGK YLSEHQVRPA DFNQYGTRRG 730 740 750 760 770 780 NHEVMMRGTF ANIRIKNFML KGADGNIPEG GLTKHWPDGE QMSIYDAAMK YQQEQVPLVV 790 800 810 820 830 840 FAGAEYGNGS SRDWAAKGTR LLGVRAVICQ SFERIHRSNL VGMGVLPLTF EEGTSWSSLG 850 860 870 880 890 900 LKGDEKVTLR GLVGDLKPRQ KLTAEIVSGD GSLQRVSLLC RIDTLDELDY YRNGGILHYV LRKLAA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Bradyrhizobium japonicum aconitase gene (acnA) is important for free-living growth but not for an effective root nodule symbiosis." Thoeny-Meyer L., Kuenzler P. J. Bacteriol. 178:6166-6172(1996) [PubMed: 8892815] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: USDA 110spc4.
[2]	Erratum Thoeny-Meyer L., Kuenzler P. J. Bacteriol. 179:1836-1836(1997)
[3]	"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U56817 Genomic DNA. Translation: AAC44562.1. BA000040 Genomic DNA. Translation: BAC45731.1.
RefSeq	NP_767106.1.
3D structure databases
HSSP	HSSP built from PDB template 7ACN based on UniProtKB P16276.
ModBase	Search...
Genome annotation databases
GeneID	1052978.
GenomeReviews	Gene locus bll0466 in contig BA000040_GR.
KEGG	bja:bll0466.
NMPDR	fig|224911.1.peg.466.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P70920.
OMA	P70920. RIKNEML.
Enzyme and pathway databases
BioCyc	BJAP224911:BLL0466-MON.
BRENDA	4.2.1.3. 280.
Family and domain databases
InterPro	IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3. IPR001030. Acoase/IPM_deHydtase_lsu_aba. IPR015937. Aconitase-like_core. IPR015928. Aconitase/3IPM_dehydase_swvl. IPR006249. Aconitase/Fe_reg_prot_2. IPR015934. Aconitase/Fe_reg_prot_2/AcnD. IPR015932. Aconitase/IPMdHydase_lsu_aba_2. IPR018136. Aconitase_4Fe-4S_BS. IPR000573. AconitaseA/IPMdHydase_ssu_swvl. [Graphical view]
Gene3D	G3DSA:3.30.499.10. Acnase/IPM_dHydase_lsu_aba_1/3. 2 hits. G3DSA:3.20.19.10. Aconitase/3IPM_dehydase_swvl. 1 hit. G3DSA:3.40.1060.10. Aconitase/IPMdHydase_lsu_aba_2. 1 hit.
PANTHER	PTHR11670. Aconitase-like_core. 1 hit. PTHR11670:SF1. Aconitase/Fe_reg_prot_2/AcnD. 1 hit.
Pfam	PF00330. Aconitase. 1 hit. PF00694. Aconitase_C. 1 hit. [Graphical view]
PRINTS	PR00415. ACONITASE.
ProDom	PD000511. Aconitase_N. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01341. aconitase_1. 1 hit.
PROSITE	PS00450. ACONITASE_1. 1 hit. PS01244. ACONITASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACON_BRAJA

Accession

Primary (citable) accession number: P70920

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 28, 2003
Last modified:	June 16, 2009
This is version 61 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)