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P70920 (ACON_BRAJA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Aconitate hydratase
Short name=Aconitase
EC=4.2.1.3
Alternative name(s):
Citrate hydro-lyase
Gene names
Name:acnA
Ordered Locus Names:bll0466
OrganismBradyrhizobium japonicum (strain USDA 110) [Reference proteome] [HAMAP]
Taxonomic identifier224911 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium ›

Protein attributes

Sequence length906 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the isomerization of citrate to isocitrate via cis-aconitate By similarity.

Catalytic activity

Citrate = isocitrate.

Cofactor

Binds 1 4Fe-4S cluster per subunit By similarity.

Pathway

Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 2/2.

Subunit structure

Monomer By similarity.

Sequence similarities

Belongs to the aconitase/IPM isomerase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 906906Aconitate hydratase
PRO_0000076656

Sites

Metal binding4431Iron-sulfur (4Fe-4S) By similarity
Metal binding5091Iron-sulfur (4Fe-4S) By similarity
Metal binding5121Iron-sulfur (4Fe-4S) By similarity

Experimental info

Sequence conflict5961W → C in AAC44562. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P70920 [UniParc].

Last modified February 28, 2003. Version 2.
Checksum: E1A9C4AFA0614104

FASTA90698,322
        10         20         30         40         50         60 
MTSLDSFKCK KTLKVGAKTY VYYSLPTAEK NGLKGISKLP YSMKVLLENL LRNEDGRSVK 

        70         80         90        100        110        120 
KADIVAVSKW LRKKSLEHEI AFRPARVLMQ DFTGVPAVVD LAAMRNAMQK LGGDAEKINP 

       130        140        150        160        170        180 
LVPVDLVIDH SVIVNFFGDN KAFAKNVTEE YKQNQERYEF LKWGQAAFSN FSVVPPGTGI 

       190        200        210        220        230        240 
CHQVNLEYLS QTVWTKKEKM TVGKKTGTFE VAYPDSLVGT DSHTTMVNGL AVLGWGVGGI 

       250        260        270        280        290        300 
EAEACMLGQP LSMLLPNVVG FKLKGAMKEG VTATDLVLTV TQMLRKLGVV GKFVEFFGPG 

       310        320        330        340        350        360 
LDHLSVADKA TIANMAPEYG ATCGFFPVDA AAIDYLKTSG RAAPRVALVQ AYAKAQGLFR 

       370        380        390        400        410        420 
TAKSADPVFT ETLTLDLADV VPSMAGPKRP EGRIALPSVA EGFSVALANE YKKTEEPAKR 

       430        440        450        460        470        480 
FAVEGKKYEI GHGDVVIAAI TSCTNTSNPS VLIGAGLLAR NAAAKGLKAK PWVKTSLAPG 

       490        500        510        520        530        540 
SQVVAAYLAD SGLQAHLDKV GFNLVGFGCT TCIGNSGPLP EEISKSINDN GIVAAAVLSG 

       550        560        570        580        590        600 
NRNFEGRVSP DVQANYLASP PLVVAHALAG SVTKNLAVEP LGEGKDGKPV YLKDIWPTSK 

       610        620        630        640        650        660 
EINAFMKKFV TASIFKKKYA DVFKGDTNWR KIKTVESETY RWNMSSTYVQ NPPYFEGMKK 

       670        680        690        700        710        720 
EPEPVTDIVE ARILAMFGDK ITTDHISPAG SIKLTSPAGK YLSEHQVRPA DFNQYGTRRG 

       730        740        750        760        770        780 
NHEVMMRGTF ANIRIKNFML KGADGNIPEG GLTKHWPDGE QMSIYDAAMK YQQEQVPLVV 

       790        800        810        820        830        840 
FAGAEYGNGS SRDWAAKGTR LLGVRAVICQ SFERIHRSNL VGMGVLPLTF EEGTSWSSLG 

       850        860        870        880        890        900 
LKGDEKVTLR GLVGDLKPRQ KLTAEIVSGD GSLQRVSLLC RIDTLDELDY YRNGGILHYV 


LRKLAA

« Hide

References

« Hide 'large scale' references
[1]"The Bradyrhizobium japonicum aconitase gene (acnA) is important for free-living growth but not for an effective root nodule symbiosis."
Thoeny-Meyer L., Kuenzler P.
J. Bacteriol. 178:6166-6172(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: USDA 110spc4.
[2]Erratum
Thoeny-Meyer L., Kuenzler P.
J. Bacteriol. 179:1836-1836(1997)
[3]"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110."
Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.
DNA Res. 9:189-197(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: USDA 110.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U56817 Genomic DNA. Translation: AAC44562.1.
BA000040 Genomic DNA. Translation: BAC45731.1.
RefSeqNP_767106.1. NC_004463.1.

3D structure databases

ProteinModelPortalP70920.
ModBaseSearch...

Protein-protein interaction databases

STRING224911.bll0466.

Proteomic databases

PRIDEP70920.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAC45731; BAC45731; BAC45731.
GeneID1052978.
KEGGbja:bll0466.
PATRIC21184397. VBIBraJap65052_0476.

Phylogenomic databases

eggNOGCOG1048.
HOGENOMHOG000025703.
KOK01681.
OMARNCDGKK.
ProtClustDBPRK09277.

Enzyme and pathway databases

BioCycBJAP224911:GJEJ-469-MONOMER.
UniPathwayUPA00223; UER00718.

Family and domain databases

Gene3D3.20.19.10. 1 hit.
3.30.499.10. 2 hits.
3.40.1060.10. 1 hit.
InterProIPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015928. Aconitase/3IPM_dehydase_swvl.
IPR006249. Aconitase/Fe_reg_prot_2.
IPR015934. Aconitase/Fe_reg_prot_2/AcnD.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
IPR000573. AconitaseA/IPMdHydase_ssu_swvl.
[Graphical view]
PANTHERPTHR11670. PTHR11670. 1 hit.
PTHR11670:SF1. PTHR11670:SF1. 1 hit.
PfamPF00330. Aconitase. 1 hit.
PF00694. Aconitase_C. 1 hit.
[Graphical view]
PRINTSPR00415. ACONITASE.
SUPFAMSSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
SSF53732. Aconitase_N. 1 hit.
TIGRFAMsTIGR01341. aconitase_1. 1 hit.
PROSITEPS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameACON_BRAJA
AccessionPrimary (citable) accession number: P70920
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 28, 2003
Last modified: May 1, 2013
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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