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Protein

Cycloisomaltooligosaccharide glucanotransferase

Gene

cit

Organism
Bacillus circulans
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Produces cycloisomaltooligosaccharide from dextran.

Catalytic activityi

Cyclizes part of a (1->6)-alpha-D-glucan chain by formation of a (1->6)-alpha-D-glucosidic bond.

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Protein family/group databases

CAZyiCBM35. Carbohydrate-Binding Module Family 35.
GH66. Glycoside Hydrolase Family 66.

Names & Taxonomyi

Protein namesi
Recommended name:
Cycloisomaltooligosaccharide glucanotransferase (EC:2.4.1.248)
Short name:
CITase
Gene namesi
Name:cit
OrganismiBacillus circulans
Taxonomic identifieri1397 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3030Sequence AnalysisAdd
BLAST
Chaini31 – 964934Cycloisomaltooligosaccharide glucanotransferasePRO_0000012242Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP70873.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini413 – 538126CBM6 1PROSITE-ProRule annotationAdd
BLAST
Domaini740 – 863124CBM6 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 66 family.Curated
Contains 2 CBM6 (carbohydrate binding type-6) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Family and domain databases

Gene3Di2.60.120.260. 2 hits.
InterProiIPR011635. APHP.
IPR005084. CMB_fam6.
IPR008979. Galactose-bd-like.
IPR025092. Glyco_hydro_66.
[Graphical view]
PfamiPF07705. CARDB. 1 hit.
PF03422. CBM_6. 2 hits.
PF13199. Glyco_hydro_66. 1 hit.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 2 hits.
PROSITEiPS51175. CBM6. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P70873-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVKILPLVF MTLLLIVPSQ MLLPSGQANA STPGFIERVY TDKARYEPGE
60 70 80 90 100
LVTVTAQINN SGGTNWSGDV TMTIFHLENA VYSSVQHASI ASGQTTDVTF
110 120 130 140 150
SWTSDTTDFK GYFVSVDAGS LGQGYSSIDV SSDFAKYPRY GYISEFSSNE
160 170 180 190 200
TAAESAAKVN ELAQDYKINA WQFYDWMWRH ETMIKRTGGT IDPTWIDLFN
210 220 230 240 250
RQISWPTINN QIAAIHNQNG AAMAYAMIYA ARENYSGFGV NPEWGMYMDP
260 270 280 290 300
AHTKQLDVDF GNNSTYMYLF DPANAGWQQF IHEQYLDAIQ TANFDGIHID
310 320 330 340 350
QMGQRNNIYD YSGNSIDLAT RFTPFIKAAK TKLTAANSNQ DFMTFNIVDG
360 370 380 390 400
TVNGWAANDV SKNANVDFLY SEIWHLSNSY MQLKDYIDSL RANSGNKAVV
410 420 430 440 450
LAAYMNYGEN IGDRYEAEDA ALQHTAVNTD HAGYTGSGFV DQFADVNDSV
460 470 480 490 500
TFTITAPEEG YYSLVFRFAN HSGYTATRNL YVDSNFEIEL PFQNQPNWDT
510 520 530 540 550
WSHETWHQVY LTPGTHTIKL SYDSSNTGAI NLDSLTLGTF DEHSIRLADA
560 570 580 590 600
MMAASGATHI ELGEDSQMLA HEYYPNRSKS MRSTLKSAMK DHYNFITAYE
610 620 630 640 650
NLLFDADVID NDAGKQFINI AGVNTSPDGA ANTVWHMSKR TPEYNILHLI
660 670 680 690 700
NLVNNDQNWR NSGNQPTAQT NLATKVYIGA EETITGVYAA SPDHNQGATQ
710 720 730 740 750
SLPFTTGTDS SGSYISFTVP SLEYWSMIYM KRSTAAPVDN MYEAETAIKS
760 770 780 790 800
NVSVNTNHAG YTGSGFVDQF ATVNDGVSFI VHASSKDDYV LRFRYSNGGS
810 820 830 840 850
DANRDVFLNG KYAGTVQLKH TGGWNQWAYG ELTVPLAQGS HSVVLWYNSS
860 870 880 890 900
NSGAVNLDHL KLDKTYIWQF DRQIASVPAG YRITFKAGLP GWVHFGTDNW
910 920 930 940 950
KNVMDIPLAS NGSSDSSLNY EASIGPFPSA TTVDVTFLWD DNNNGILEDM
960
IDRWEGTDFQ IAIP
Length:964
Mass (Da):107,208
Last modified:February 1, 1997 - v1
Checksum:i8849CDC5E2DE9A68
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D88360 Genomic DNA. Translation: BAA13595.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D88360 Genomic DNA. Translation: BAA13595.1.

3D structure databases

ProteinModelPortaliP70873.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiCBM35. Carbohydrate-Binding Module Family 35.
GH66. Glycoside Hydrolase Family 66.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di2.60.120.260. 2 hits.
InterProiIPR011635. APHP.
IPR005084. CMB_fam6.
IPR008979. Galactose-bd-like.
IPR025092. Glyco_hydro_66.
[Graphical view]
PfamiPF07705. CARDB. 1 hit.
PF03422. CBM_6. 2 hits.
PF13199. Glyco_hydro_66. 1 hit.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 2 hits.
PROSITEiPS51175. CBM6. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Purification and some properties of cycloisomaltooligosaccharide glucanotransferase and cloning of cit gene from Bacillus circulans U-155."
    Oguma T., Kurokawa T., Tobe K., Kitao S., Kobayashi M.
    Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: U-155.

Entry informationi

Entry nameiCTA2_BACCI
AccessioniPrimary (citable) accession number: P70873
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 1, 1997
Last modified: October 1, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.