Reviewed, UniProtKB/Swiss-Prot P70873 (CTA2_BACCI)

Names and origin

Protein names

Recommended name:
    Cycloisomaltooligosaccharide glucanotransferase
      Short name=CITase
    EC=2.4.1.-

Gene names

Name:

cit

Organism

Bacillus circulans

Taxonomic identifier

1397 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	964 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Produces cycloisomaltooligosaccharide from dextran.
Sequence similarities	Belongs to the glycosyl hydrolase 66 family. Contains 2 CBM6 (carbohydrate binding type-6) domains.

Ontologies

Keywords
Domain	Repeat Signal
Molecular function	Glycosyltransferase Transferase
Gene Ontology (GO)
Molecular function	carbohydrate binding Inferred from electronic annotation. Source: InterPro transferase activity, transferring glycosyl groups Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 30	30	Potential
	Chain	31 – 964	934	Cycloisomaltooligosaccharide glucanotransferase		PRO_0000012242
Regions
	Domain	413 – 538	126	CBM6 1
	Domain	740 – 863	124	CBM6 2

Sequences

	Sequence		Length	Mass (Da)
	P70873-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 8849CDC5E2DE9A68	FASTA	964	107,208
	10 20 30 40 50 60 MRVKILPLVF MTLLLIVPSQ MLLPSGQANA STPGFIERVY TDKARYEPGE LVTVTAQINN 70 80 90 100 110 120 SGGTNWSGDV TMTIFHLENA VYSSVQHASI ASGQTTDVTF SWTSDTTDFK GYFVSVDAGS 130 140 150 160 170 180 LGQGYSSIDV SSDFAKYPRY GYISEFSSNE TAAESAAKVN ELAQDYKINA WQFYDWMWRH 190 200 210 220 230 240 ETMIKRTGGT IDPTWIDLFN RQISWPTINN QIAAIHNQNG AAMAYAMIYA ARENYSGFGV 250 260 270 280 290 300 NPEWGMYMDP AHTKQLDVDF GNNSTYMYLF DPANAGWQQF IHEQYLDAIQ TANFDGIHID 310 320 330 340 350 360 QMGQRNNIYD YSGNSIDLAT RFTPFIKAAK TKLTAANSNQ DFMTFNIVDG TVNGWAANDV 370 380 390 400 410 420 SKNANVDFLY SEIWHLSNSY MQLKDYIDSL RANSGNKAVV LAAYMNYGEN IGDRYEAEDA 430 440 450 460 470 480 ALQHTAVNTD HAGYTGSGFV DQFADVNDSV TFTITAPEEG YYSLVFRFAN HSGYTATRNL 490 500 510 520 530 540 YVDSNFEIEL PFQNQPNWDT WSHETWHQVY LTPGTHTIKL SYDSSNTGAI NLDSLTLGTF 550 560 570 580 590 600 DEHSIRLADA MMAASGATHI ELGEDSQMLA HEYYPNRSKS MRSTLKSAMK DHYNFITAYE 610 620 630 640 650 660 NLLFDADVID NDAGKQFINI AGVNTSPDGA ANTVWHMSKR TPEYNILHLI NLVNNDQNWR 670 680 690 700 710 720 NSGNQPTAQT NLATKVYIGA EETITGVYAA SPDHNQGATQ SLPFTTGTDS SGSYISFTVP 730 740 750 760 770 780 SLEYWSMIYM KRSTAAPVDN MYEAETAIKS NVSVNTNHAG YTGSGFVDQF ATVNDGVSFI 790 800 810 820 830 840 VHASSKDDYV LRFRYSNGGS DANRDVFLNG KYAGTVQLKH TGGWNQWAYG ELTVPLAQGS 850 860 870 880 890 900 HSVVLWYNSS NSGAVNLDHL KLDKTYIWQF DRQIASVPAG YRITFKAGLP GWVHFGTDNW 910 920 930 940 950 960 KNVMDIPLAS NGSSDSSLNY EASIGPFPSA TTVDVTFLWD DNNNGILEDM IDRWEGTDFQ IAIP

References

[1]

"Purification and some properties of cycloisomaltooligosaccharide glucanotransferase and cloning of cit gene from Bacillus circulans U-155." Oguma T., Kurokawa T., Tobe K., Kitao S., Kobayashi M. Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: U-155.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D88360 Genomic DNA. Translation: BAA13595.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	CBM35. Carbohydrate-Binding Module Family 35. GH66. Glycoside Hydrolase Family 66.
Family and domain databases
InterPro	IPR005084. CBM_6. [Graphical view]
Pfam	PF03422. CBM_6. 2 hits. [Graphical view]
PROSITE	PS51175. CBM6. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CTA2_BACCI

Accession

Primary (citable) accession number: P70873

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	February 1, 1997
Last modified:	April 14, 2009
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)