P70870 (RLUD_BORBU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal large subunit pseudouridine synthase D
EC=5.4.99.23

Alternative name(s):
23S rRNA pseudouridine(1911/1915/1917) synthase
rRNA pseudouridylate synthase D
rRNA-uridine isomerase D

Gene names

Name:	rluD
Ordered Locus Names:	BB_0018

Organism

Borrelia burgdorferi (Lyme disease spirochete)

Taxonomic identifier

139 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Borrelia › Borrelia burgdorferi group

Protein attributes

Sequence length	326 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Responsible for synthesis of pseudouridine from uracil at positions 1911, 1915 and 1917 in 23S ribosomal RNA By similarity.
Catalytic activity	23S rRNA uridine(1911)/uridine(1915)/uridine(1917) = 23S rRNA pseudouridine(1911)/pseudouridine(1915)/pseudouridine(1917).
Sequence similarities	Belongs to the pseudouridine synthase rluA family.
Sequence caution	The sequence AAC66413.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	rRNA processing
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	pseudouridine synthesis Inferred from electronic annotation. Source: InterPro rRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro pseudouridine synthase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 326	326	Ribosomal large subunit pseudouridine synthase D		PRO_0000162683
Sites
	Active site	144	1	By similarity
Experimental info
	Sequence conflict	172	1	T → I in CAA70352. Ref.1
	Sequence conflict	268	1	K → E in CAA70352. Ref.1
	Sequence conflict	299 – 326	28	FVIFL…FLRDF → ICYFFVKFLQE in CAA70352. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P70870 [UniParc]. Last modified December 15, 1998. Version 2. Checksum: 52266C0AF8BFEA3C	FASTA	326	38,067
	10 20 30 40 50 60 MIRVKREFTV KENALRLDLY LSGNFEVFTR SQIKRRNVEA FKKSNGKFLK IKLSKPVFKD 70 80 90 100 110 120 DEILIEFDEE SSQIDCLRPS NIPIAIIYED SNVIVLNKPQ GILSHPGISH WDDTVVNFLL 130 140 150 160 170 180 YHIKSLKINF NEEKIRPGIV HRLDKDTSGV LICAKNISTL RFLAQQFKDK RTNKVYIAIV 190 200 210 220 230 240 KGNFNSFFGS IESFIDRDKH NRKKFSVSKD RGKKALTEYR LLLNFGEYSL LALKPKTGRT 250 260 270 280 290 300 HQLRVHMKYL NFPILGDEVY GRVDGSLKKI TLMLHSYKLE IDVGKNSFKK FISEFPKRFV 310 320 IFLSNFYKSD ELNLIIDNLV LFLRDF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Boursaux-Eude C., Margarita D., Belfaiza J., Old I.G., Saint-Girons I. Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: HB19.
[2]	"Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi." Fraser C.M., Casjens S., Huang W.M., Sutton G.G., Clayton R.A., Lathigra R., White O., Ketchum K.A., Dodson R.J., Hickey E.K., Gwinn M.L., Dougherty B.A., Tomb J.-F., Fleischmann R.D., Richardson D.L., Peterson J.D., Kerlavage A.R., Quackenbush J. , Salzberg S.L., Hanson M., van Vugt R., Palmer N., Adams M.D., Gocayne J.D., Weidman J.F., Utterback T.R., Watthey L., McDonald L.A., Artiach P., Bowman C., Garland S.A., Fujii C., Cotton M.D., Horst K., Roberts K.M., Hatch B., Smith H.O., Venter J.C. Nature 390:580-586(1997) [PubMed: 9403685] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35210 / B31 / CIP 102532 / DSM 4680.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y09142 Genomic DNA. Translation: CAA70352.1. AE000783 Genomic DNA. Translation: AAC66413.1. Different initiation.
PIR	B70102.
RefSeq	NP_212152.1. NC_001318.1.
3D structure databases
ProteinModelPortal	P70870.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBORT00000009172; EBBORP00000008502; EBBORG00000009171.
GeneID	1194854.
GenomeReviews	Gene locus BB_0018 in contig AE000783_GR.
KEGG	bbu:BB0018.
NMPDR	fig|224326.1.peg.402.
PATRIC	20556639. VBIBorBur75917_0416.
TIGR	BB_0018.
Phylogenomic databases
GeneTree	EBGT00050000007125.
HOGENOM	HBG750656.
OMA	PQGILSH.
PhylomeDB	P70870.
ProtClustDB	CLSK507297.
Enzyme and pathway databases
BioCyc	BBUR224326:BB_0018-MONOMER.
Family and domain databases
InterPro	IPR020103. PsdUridine_synth_cat_dom. IPR006225. PsdUridine_synth_RluC/D. IPR006224. PsdUridine_synth_RluC/D_CS. IPR006145. PsdUridine_synth_RsuA/RluD. [Graphical view]
KO	K06180.
Pfam	PF00849. PseudoU_synth_2. 1 hit. [Graphical view]
SUPFAM	SSF55120. PsdUridine_synth_cat_dom. 1 hit.
TIGRFAMs	TIGR00005. RluA_subfam. 1 hit.
PROSITE	PS01129. PSI_RLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RLUD_BORBU

Accession

Primary (citable) accession number: P70870
Secondary accession number(s): O51050

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	December 15, 1998
Last modified:	January 25, 2012
This is version 70 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program