P70830 (TRUA_BORBU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA pseudouridine synthase A
EC=5.4.99.12

Alternative name(s):
tRNA pseudouridine(38-40) synthase
tRNA pseudouridylate synthase I
tRNA-uridine isomerase I

Gene names

Name:	truA
Ordered Locus Names:	BB_0012

Organism

Borrelia burgdorferi (Lyme disease spirochete)

Taxonomic identifier

139 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Borrelia › Borrelia burgdorferi group

Protein attributes

Sequence length	246 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs By similarity. HAMAP MF_00171
Catalytic activity	tRNA uridine(38-40) = tRNA pseudouridine(38-40). HAMAP MF_00171
Subunit structure	Homodimer By similarity. HAMAP MF_00171
Sequence similarities	Belongs to the tRNA pseudouridine synthase TruA family.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	pseudouridine synthesis Inferred from electronic annotation. Source: InterPro tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro pseudouridine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 246	246	tRNA pseudouridine synthase A HAMAP MF_00171		PRO_0000057341
Sites
	Active site	52	1	Nucleophile By similarity
	Binding site	111	1	Substrate By similarity
Experimental info
	Sequence conflict	59	1	K → R in CAA70351. Ref.1
	Sequence conflict	59	1	K → R in CAA11967. Ref.1
	Sequence conflict	201	1	I → M in CAA70351. Ref.1
	Sequence conflict	201	1	I → M in CAA11967. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P70830 [UniParc]. Last modified December 15, 1998. Version 2. Checksum: B1F408385FF171A7	FASTA	246	28,657
	10 20 30 40 50 60 MKKILAEIAY DGSIYHGFQI QPTKPTVQGE IEKALMKINK KKVKIHSSGR TDKGVHAKKQ 70 80 90 100 110 120 IITFDIKINI QLNNLKKALN AILLKNSIKI LKLRYVKNSF HPRFSAQKRK YSYCILNSDN 130 140 150 160 170 180 YYPWEGYQAH YVNKKLSISN LNQMAKTLIG KHDFTTFSCI KDKSKSKFRH IYFAKFKKRG 190 200 210 220 230 240 KYIIFEIIGS SFLWKMVRSI IGTMLDIEIK NESISTFETI LKSKNRNLAR TTAPANALFL ERVYYE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Boursaux-Eude C., Margarita D., Belfaiza J., Old I.G., Saint-Girons I. Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: HB19.
[2]	"Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi." Fraser C.M., Casjens S., Huang W.M., Sutton G.G., Clayton R.A., Lathigra R., White O., Ketchum K.A., Dodson R.J., Hickey E.K., Gwinn M.L., Dougherty B.A., Tomb J.-F., Fleischmann R.D., Richardson D.L., Peterson J.D., Kerlavage A.R., Quackenbush J. , Salzberg S.L., Hanson M., van Vugt R., Palmer N., Adams M.D., Gocayne J.D., Weidman J.F., Utterback T.R., Watthey L., McDonald L.A., Artiach P., Bowman C., Garland S.A., Fujii C., Cotton M.D., Horst K., Roberts K.M., Hatch B., Smith H.O., Venter J.C. Nature 390:580-586(1997) [PubMed: 9403685] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35210 / B31 / CIP 102532 / DSM 4680.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y09141 Genomic DNA. Translation: CAA70351.1. AJ224474 Genomic DNA. Translation: CAA11967.1. AE000783 Genomic DNA. Translation: AAC66394.1.
PIR	D70101.
RefSeq	NP_212146.1. NC_001318.1.
3D structure databases
ProteinModelPortal	P70830.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBORT00000009077; EBBORP00000008407; EBBORG00000009076.
GeneID	1194848.
GenomeReviews	Gene locus BB_0012 in contig AE000783_GR.
KEGG	bbu:BB0012.
NMPDR	fig|224326.1.peg.396.
PATRIC	20556627. VBIBorBur75917_0410.
TIGR	BB_0012.
Phylogenomic databases
GeneTree	EBGT00050000007466.
HOGENOM	HBG621020.
OMA	QANAFVH.
PhylomeDB	P70830.
ProtClustDB	PRK00021.
Enzyme and pathway databases
BioCyc	BBUR224326:BB_0012-MONOMER.
Family and domain databases
HAMAP	MF_00171. TruA. [Tree]
InterPro	IPR020103. PsdUridine_synth_cat_dom. IPR001406. PsdUridine_synth_TruA. IPR020097. PsdUridine_synth_TruA_a/b_dom. IPR020095. PsdUridine_synth_TruA_C. IPR020094. PsdUridine_synth_TruA_N. [Graphical view]
Gene3D	G3DSA:3.30.70.580. PseudoU_synth_1. 1 hit. G3DSA:3.30.70.660. PseudoU_synth_1. 1 hit.
KO	K06173.
PANTHER	PTHR11142. PseudoU_synth_1. 1 hit.
Pfam	PF01416. PseudoU_synth_1. 2 hits. [Graphical view]
PIRSF	PIRSF001430. tRNA_psdUrid_synth. 1 hit.
SUPFAM	SSF55120. PsdUridine_synth_cat_dom. 1 hit.
TIGRFAMs	TIGR00071. HisT_truA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRUA_BORBU

Accession

Primary (citable) accession number: P70830
Secondary accession number(s): O51045

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	December 15, 1998
Last modified:	January 25, 2012
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program