Pyrophosphate-fructose 6-phosphate 1-phosphotransferase - Borrelia burgdorferi (Lyme disease spirochete)

Preferred order of sections

Names and origin

Protein names

Submitted name:
Pyrophosphate-fructose 6-phosphate 1-phosphotransferase EMBL CAA11968.1
EC=2.7.1.90 EMBL CAA11968.1

Gene names

Name:

pfpB EMBL CAA11968.1

Organism

Borrelia burgdorferi (Lyme disease spirochete) EMBL CAA11968.1

Taxonomic identifier

139 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Borrelia › Borrelia burgdorferi group

Protein attributes

Sequence length	555 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Molecular function	Transferase EMBL CAA11968.1
Technical term	3D-structure PDB 1KZH PDB 2F48
Gene Ontology (GO)
Biological_process	fructose 6-phosphate metabolic process Inferred from electronic annotation. Source: InterPro glycolysis Inferred from electronic annotation. Source: InterPro
Cellular_component	6-phosphofructokinase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	6-phosphofructokinase activity Inferred from electronic annotation. Source: InterPro ATP binding Inferred from electronic annotation. Source: InterPro diphosphate-fructose-6-phosphate 1-phosphotransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Sites

Binding site

206

1

Substrate PDB 1KZH

Site

82

1

Transition state stabilizer; via amide nitrogen PDB 1KZH

Site

146

1

Transition state stabilizer PDB 1KZH

Site

203

1

Transition state stabilizer PDB 1KZH

Site

204

1

Transition state stabilizer PDB 1KZH

Sequences

Sequence

Length

Mass (Da)

Tools

P70826 [UniParc].

Last modified November 1, 1998. Version 2.
Checksum: 0FEAC1077DA6127F
Show »

FASTA

555

62,443

        10         20         30         40         50         60 
MNTSLFKQER QKYIPKLPNI LKKDFNNISL VYGENTEAIQ DRQALKEFFK NTYGLPIISF 

        70         80         90        100        110        120 
TEGESSLSFS KALNIGIILS GGPAPGGHNV ISGVFDAIKK FNPNSKLFGF KGGPLGLLEN 

       130        140        150        160        170        180 
DKIELTESLI NSYRNTGGFD IVSSGRTKIE TEEHYNKALF VAKENNLNAI IIIGGDDSNT 

       190        200        210        220        230        240 
NAAILAEYFK KNGENIQVIG VPKTIDADLR NDHIEISFGF DSATKIYSEL IGNLCRDAMS 

       250        260        270        280        290        300 
TKKYWHFVKL MGRSASHVAL ECALKTHPNI CIVSEEVLAK KKTLSEIIDE MVSVILKRSL 

       310        320        330        340        350        360 
NGDNFGVVIV PEGLIEFIPE VKSLMLELCD IFDKNEGEFK GLNIEKMKEI FVAKLSDYMK 

       370        380        390        400        410        420 
GVYLSLPLFI QFELIKSILE RDPHGNFNVS RVPTEKLFIE MIQSRLNDMK KRGEYKGSFT 

       430        440        450        460        470        480 
PVDHFFGYEG RSAFPSNFDS DYCYSLGYNA VVLILNGLTG YMSCIKNLNL KPTDWIAGGV 

       490        500        510        520        530        540 
PLTMLMNMEE RYGEKKPVIK KALVDLEGRP FKEFVKNRDK WALNNLYLYP GPVQYFGSSE 

       550 
IVDEITETLK LELLK

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References

[1]	"Homologues of helicase genes priA and ruvAB of Borrelia burgdorferi, the Lyme borreliosis agent." Boursaux-Eude C., Margarita D., Belfaiza J., Old I.G., Saint-Girons I. Res. Microbiol. 149:235-245(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: HB19 EMBL CAA11968.1.
[2]	"The structure of a pyrophosphate-dependent phosphofructokinase from the Lyme disease spirochete Borrelia burgdorferi." Moore S.A., Ronimus R.S., Roberson R.S., Morgan H.W. Structure 10:659-671(2002) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.55 ANGSTROMS) OF 1-553, ACTIVE SITE.
[3]	"Crystal Structure of A Novel Fructose 1,6-Bisphosphate and AlF3 containing Pyrophosphate-dependent Phosphofructo-1-kinase Complex from Borreliaburgdorferi." Wang M., Grum-Tokars V. Submitted (NOV-2005) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.11 ANGSTROMS) OF 1-553.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AJ224475 Genomic DNA. Translation: CAA11968.1.

PIR

D70102.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1KZH	X-ray	2.55	A/B	1-553	[»]
2F48	X-ray	2.11	A/B	1-553	[»]

ProteinModelPortal

P70826.

SMR

P70826. Positions 4-553.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

1.10.10.480. 1 hit.

InterPro

IPR011183. PfpB_PPi_PFK.
IPR022953. Phosphofructokinase.
IPR000023. Phosphofructokinase_dom.
IPR013981. PPi-dep_PFK_a-hlx.
[Graphical view]

Pfam

PF00365. PFK. 1 hit.
[Graphical view]

PIRSF

PIRSF005677. PPi_PFK_PfpB. 1 hit.

PRINTS

PR00476. PHFRCTKINASE.

SUPFAM

SSF53784. Ppfruckinase. 1 hit.

TIGRFAMs

TIGR02477. PFKA_PPi. 1 hit.

ProtoNet

Search...

Entry information

Entry name

P70826_BORBG

Accession

Primary (citable) accession number: P70826

Entry history

Integrated into UniProtKB/TrEMBL:	February 1, 1997
Last sequence update:	November 1, 1998
Last modified:	April 3, 2013
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)