Reviewed, UniProtKB/Swiss-Prot P70753 (BGAL_ACTPL)

Names and origin

Protein names

Recommended name:
    Beta-galactosidase
      Short name=Beta-gal
    EC=3.2.1.23
Alternative name(s):
    Lactase

Gene names

Name:

lacZ

Organism

Actinobacillus pleuropneumoniae (Haemophilus pleuropneumoniae)

Taxonomic identifier

715 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	1005 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.
Sequence similarities	Belongs to the glycosyl hydrolase 2 family.

Ontologies

Keywords
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	beta-galactosidase complex Inferred from electronic annotation. Source: InterPro
Molecular function	beta-galactosidase activity Inferred from electronic annotation. Source: EC carbohydrate binding Inferred from electronic annotation. Source: InterPro cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1005	1005	Beta-galactosidase		PRO_0000057654
Sites
	Active site	455	1	Proton donor By similarity
	Active site	526	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	P70753-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 12254482581C710E	FASTA	1,005	117,009
	10 20 30 40 50 60 MILPNYFQDP NTLHVNTVEH HAYFIPHQQN ETALSGKREQ SDYFTLLNGQ WDFNYFQSYH 70 80 90 100 110 120 DLPDNFLDIA FEHKIPVPAN WQNHGFDHHH YTNINYPFPF EPPFVPHQNP CGVYHRTLQL 130 140 150 160 170 180 TPKHKRYLLN FEGVDSCLFV YVNKQFVGYS QISHNTSEFD VSDYLQAGTN HLTVVVLKWC 190 200 210 220 230 240 DGSYLEDQDK FRMSGIFRDV YLLEREQNYL QDFFIQYELD DELRHANLKV ETLFSRQPQA 250 260 270 280 290 300 IEYQLLNPNG FTVFNQTDTH LNIEVEDIQL WNAEKPQLYT LILHTAEEVI VQKIGFRKVE 310 320 330 340 350 360 IKDGILLFNQ QPIKFKGVNR HDSDPKTGYA ITYAQAHKDL QLMKQHNINA IRTAHYPNAP 370 380 390 400 410 420 WFSELCDQYG FYLIGESDVE SHGASMLTVK MPEPSILLNH QNDLQTERIR QDMIDNYCYF 430 440 450 460 470 480 ARDPLFKKAI LDRQQANVER DKNRTSIIIW SLGNEAGYGA NFEAAAAWIK QRDKSRLVHY 490 500 510 520 530 540 ESSIYQHSAD NNDLSNLDFY SEMYGSTEDI DRYCATAQRK PFVLCEYSHA MGNSNGDAED 550 560 570 580 590 600 YWQAFHRHPQ SCGGFVWEWC DHAPYRANGQ FGYGGDFGES PHDGNFCMDG LVSPDRIPHS 610 620 630 640 650 660 NLLELKNVNR PARAELIDNQ IVIHNYLDFT DLADYLTIDY EFVENGVVTS GGNLSVSCKP 670 680 690 700 710 720 HSSVILPIEL PKNNGHLWLL NLDYRLNTAT ELLEAEHSLG FEQLNLFSEN KLVLPKFTIE 730 740 750 760 770 780 KSTFEVQEDH FRINVHNGQF SYQLDKQKGI FSRIEKAGKA IIQQPLDFNI WRAPTDNDRL 790 800 810 820 830 840 IREAWQNAGY DKAYTRAYEI QWQQSEQAVE FSVKSAIVSI SRGRILTLDI RYRIFNDGQI 850 860 870 880 890 900 SVEINAIRPT ELPYLPRFGL RFWLAKAENT VEYFGYGEQE SYVDKHHLAN LGIYHTTAKQ 910 920 930 940 950 960 NHTDYVKPQE NGSHYGCEYL KSENLFVSAS QPFSFNLSPY TQEELTEKKH YYELQESDYS 970 980 990 1000 VLCIDYKMSG IGSNSCGPNL KDQYRLMESE FKIRFDLIFD KTIRQ

References

[1]	"Expression and phylogenetic relationships of a novel lacZ homologue from Actinobacillus pleuropneumoniae." Anderson T.J., Macinnes J.I. FEMS Microbiol. Lett. 152:117-123(1997) [PubMed: 9228778] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: CM5 / Serotype 1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U62625 Genomic DNA. Translation: AAB17954.1.
PIR	T31333.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH2. Glycoside Hydrolase Family 2.
Enzyme and pathway databases
BRENDA	3.2.1.23. 257522.
Family and domain databases
InterPro	IPR008979. Galactose-bd-like. IPR011013. Glyco_hydro-type_carb-bd. IPR014718. Glyco_hydro-type_carb-bd_sub. IPR006101. Glyco_hydro_2. IPR013812. Glyco_hydro_2/20_Ig-like. IPR006104. Glyco_hydro_2_carb-bd. IPR006102. Glyco_hydro_2_Ig-like. IPR006103. Glyco_hydro_2_TIM. IPR004199. Glyco_hydro_42_D5. IPR017853. Glyco_hydro_catalytic_core. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:2.60.40.320. Glyco_hydro_2/20_Ig-like. 2 hits. G3DSA:2.70.98.10. Glyco_hydro_42_D5. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF02929. Bgal_small_N. 1 hit. PF00703. Glyco_hydro_2. 1 hit. PF02836. Glyco_hydro_2_C. 1 hit. PF02837. Glyco_hydro_2_N. 1 hit. [Graphical view]
PRINTS	PR00132. GLHYDRLASE2.
PROSITE	PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit. PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGAL_ACTPL

Accession

Primary (citable) accession number: P70753

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	February 1, 1997
Last modified:	November 24, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)