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Protein

Beta-galactosidase

Gene

lacZ

Organism
Actinobacillus pleuropneumoniae (Haemophilus pleuropneumoniae)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei455 – 4551Proton donorBy similarity
Active sitei526 – 5261NucleophileBy similarity

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase (EC:3.2.1.23)
Short name:
Beta-gal
Alternative name(s):
Lactase
Gene namesi
Name:lacZ
OrganismiActinobacillus pleuropneumoniae (Haemophilus pleuropneumoniae)
Taxonomic identifieri715 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeActinobacillus

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10051005Beta-galactosidasePRO_0000057654Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP70753.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.Curated

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 2 hits.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P70753-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MILPNYFQDP NTLHVNTVEH HAYFIPHQQN ETALSGKREQ SDYFTLLNGQ
60 70 80 90 100
WDFNYFQSYH DLPDNFLDIA FEHKIPVPAN WQNHGFDHHH YTNINYPFPF
110 120 130 140 150
EPPFVPHQNP CGVYHRTLQL TPKHKRYLLN FEGVDSCLFV YVNKQFVGYS
160 170 180 190 200
QISHNTSEFD VSDYLQAGTN HLTVVVLKWC DGSYLEDQDK FRMSGIFRDV
210 220 230 240 250
YLLEREQNYL QDFFIQYELD DELRHANLKV ETLFSRQPQA IEYQLLNPNG
260 270 280 290 300
FTVFNQTDTH LNIEVEDIQL WNAEKPQLYT LILHTAEEVI VQKIGFRKVE
310 320 330 340 350
IKDGILLFNQ QPIKFKGVNR HDSDPKTGYA ITYAQAHKDL QLMKQHNINA
360 370 380 390 400
IRTAHYPNAP WFSELCDQYG FYLIGESDVE SHGASMLTVK MPEPSILLNH
410 420 430 440 450
QNDLQTERIR QDMIDNYCYF ARDPLFKKAI LDRQQANVER DKNRTSIIIW
460 470 480 490 500
SLGNEAGYGA NFEAAAAWIK QRDKSRLVHY ESSIYQHSAD NNDLSNLDFY
510 520 530 540 550
SEMYGSTEDI DRYCATAQRK PFVLCEYSHA MGNSNGDAED YWQAFHRHPQ
560 570 580 590 600
SCGGFVWEWC DHAPYRANGQ FGYGGDFGES PHDGNFCMDG LVSPDRIPHS
610 620 630 640 650
NLLELKNVNR PARAELIDNQ IVIHNYLDFT DLADYLTIDY EFVENGVVTS
660 670 680 690 700
GGNLSVSCKP HSSVILPIEL PKNNGHLWLL NLDYRLNTAT ELLEAEHSLG
710 720 730 740 750
FEQLNLFSEN KLVLPKFTIE KSTFEVQEDH FRINVHNGQF SYQLDKQKGI
760 770 780 790 800
FSRIEKAGKA IIQQPLDFNI WRAPTDNDRL IREAWQNAGY DKAYTRAYEI
810 820 830 840 850
QWQQSEQAVE FSVKSAIVSI SRGRILTLDI RYRIFNDGQI SVEINAIRPT
860 870 880 890 900
ELPYLPRFGL RFWLAKAENT VEYFGYGEQE SYVDKHHLAN LGIYHTTAKQ
910 920 930 940 950
NHTDYVKPQE NGSHYGCEYL KSENLFVSAS QPFSFNLSPY TQEELTEKKH
960 970 980 990 1000
YYELQESDYS VLCIDYKMSG IGSNSCGPNL KDQYRLMESE FKIRFDLIFD

KTIRQ
Length:1,005
Mass (Da):117,009
Last modified:February 1, 1997 - v1
Checksum:i12254482581C710E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U62625 Genomic DNA. Translation: AAB17954.1.
PIRiT31333.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U62625 Genomic DNA. Translation: AAB17954.1.
PIRiT31333.

3D structure databases

ProteinModelPortaliP70753.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 2 hits.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Expression and phylogenetic relationships of a novel lacZ homologue from Actinobacillus pleuropneumoniae."
    Anderson T.J., Macinnes J.I.
    FEMS Microbiol. Lett. 152:117-123(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: CM5 / Serotype 1.

Entry informationi

Entry nameiBGAL_ACTPL
AccessioniPrimary (citable) accession number: P70753
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: February 1, 1997
Last modified: October 1, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.