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P70753 (BGAL_ACTPL) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-galactosidase

Short name=Beta-gal
EC=3.2.1.23
Alternative name(s):
Lactase
Gene names
Name:lacZ
OrganismActinobacillus pleuropneumoniae (Haemophilus pleuropneumoniae)
Taxonomic identifier715 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeActinobacillus

Protein attributes

Sequence length1005 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sequence similarities

Belongs to the glycosyl hydrolase 2 family.

Ontologies

Keywords
   Molecular functionGlycosidase
Hydrolase
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentbeta-galactosidase complex

Inferred from electronic annotation. Source: InterPro

   Molecular_functionbeta-galactosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

carbohydrate binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10051005Beta-galactosidase
PRO_0000057654

Sites

Active site4551Proton donor By similarity
Active site5261Nucleophile By similarity

Sequences

Sequence LengthMass (Da)Tools
P70753 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 12254482581C710E

FASTA1,005117,009
        10         20         30         40         50         60 
MILPNYFQDP NTLHVNTVEH HAYFIPHQQN ETALSGKREQ SDYFTLLNGQ WDFNYFQSYH 

        70         80         90        100        110        120 
DLPDNFLDIA FEHKIPVPAN WQNHGFDHHH YTNINYPFPF EPPFVPHQNP CGVYHRTLQL 

       130        140        150        160        170        180 
TPKHKRYLLN FEGVDSCLFV YVNKQFVGYS QISHNTSEFD VSDYLQAGTN HLTVVVLKWC 

       190        200        210        220        230        240 
DGSYLEDQDK FRMSGIFRDV YLLEREQNYL QDFFIQYELD DELRHANLKV ETLFSRQPQA 

       250        260        270        280        290        300 
IEYQLLNPNG FTVFNQTDTH LNIEVEDIQL WNAEKPQLYT LILHTAEEVI VQKIGFRKVE 

       310        320        330        340        350        360 
IKDGILLFNQ QPIKFKGVNR HDSDPKTGYA ITYAQAHKDL QLMKQHNINA IRTAHYPNAP 

       370        380        390        400        410        420 
WFSELCDQYG FYLIGESDVE SHGASMLTVK MPEPSILLNH QNDLQTERIR QDMIDNYCYF 

       430        440        450        460        470        480 
ARDPLFKKAI LDRQQANVER DKNRTSIIIW SLGNEAGYGA NFEAAAAWIK QRDKSRLVHY 

       490        500        510        520        530        540 
ESSIYQHSAD NNDLSNLDFY SEMYGSTEDI DRYCATAQRK PFVLCEYSHA MGNSNGDAED 

       550        560        570        580        590        600 
YWQAFHRHPQ SCGGFVWEWC DHAPYRANGQ FGYGGDFGES PHDGNFCMDG LVSPDRIPHS 

       610        620        630        640        650        660 
NLLELKNVNR PARAELIDNQ IVIHNYLDFT DLADYLTIDY EFVENGVVTS GGNLSVSCKP 

       670        680        690        700        710        720 
HSSVILPIEL PKNNGHLWLL NLDYRLNTAT ELLEAEHSLG FEQLNLFSEN KLVLPKFTIE 

       730        740        750        760        770        780 
KSTFEVQEDH FRINVHNGQF SYQLDKQKGI FSRIEKAGKA IIQQPLDFNI WRAPTDNDRL 

       790        800        810        820        830        840 
IREAWQNAGY DKAYTRAYEI QWQQSEQAVE FSVKSAIVSI SRGRILTLDI RYRIFNDGQI 

       850        860        870        880        890        900 
SVEINAIRPT ELPYLPRFGL RFWLAKAENT VEYFGYGEQE SYVDKHHLAN LGIYHTTAKQ 

       910        920        930        940        950        960 
NHTDYVKPQE NGSHYGCEYL KSENLFVSAS QPFSFNLSPY TQEELTEKKH YYELQESDYS 

       970        980        990       1000 
VLCIDYKMSG IGSNSCGPNL KDQYRLMESE FKIRFDLIFD KTIRQ 

« Hide

References

[1]"Expression and phylogenetic relationships of a novel lacZ homologue from Actinobacillus pleuropneumoniae."
Anderson T.J., Macinnes J.I.
FEMS Microbiol. Lett. 152:117-123(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: CM5 / Serotype 1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U62625 Genomic DNA. Translation: AAB17954.1.
PIRT31333.

3D structure databases

ProteinModelPortalP70753.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
InterProIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSPR00132. GLHYDRLASE2.
SMARTSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 2 hits.
SSF74650. SSF74650. 1 hit.
PROSITEPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGAL_ACTPL
AccessionPrimary (citable) accession number: P70753
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: February 1, 1997
Last modified: October 16, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries