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Protein

Signal recognition particle 54 kDa protein

Gene

srp54

Organism
Acidianus ambivalens (Desulfurolobus ambivalens)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi105 – 1128GTPUniRule annotation
Nucleotide bindingi187 – 1915GTPUniRule annotation
Nucleotide bindingi247 – 2504GTPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Ligandi

GTP-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Signal recognition particle 54 kDa proteinUniRule annotation
Short name:
SRP54UniRule annotation
Gene namesi
Name:srp54UniRule annotation
Synonyms:ffh
OrganismiAcidianus ambivalens (Desulfurolobus ambivalens)
Taxonomic identifieri2283 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeAcidianus

Subcellular locationi

  • Cytoplasm UniRule annotation

  • Note: The SRP-RNC complex is targeted to the cytoplasmic membrane.UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Signal recognition particle

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – 451›451Signal recognition particle 54 kDa proteinPRO_0000101171Add
BLAST

Proteomic databases

PRIDEiP70722.

Interactioni

Subunit structurei

Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY. Archaeal SRP consists of a 7S RNA molecule of 300 nucleotides and two protein subunits: SRP54 and SRP19.UniRule annotation

Structurei

Secondary structure

1
451
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 1512Combined sources
Helixi21 – 3818Combined sources
Helixi43 – 5917Combined sources
Helixi68 – 8316Combined sources
Beta strandi95 – 10410Combined sources
Helixi113 – 12412Combined sources
Beta strandi129 – 1335Combined sources
Beta strandi137 – 1393Combined sources
Helixi140 – 15213Combined sources
Helixi165 – 17814Combined sources
Beta strandi182 – 1876Combined sources
Helixi197 – 21115Combined sources
Beta strandi214 – 2218Combined sources
Helixi222 – 2287Combined sources
Helixi229 – 2379Combined sources
Beta strandi242 – 2476Combined sources
Helixi249 – 2513Combined sources
Helixi255 – 2639Combined sources
Turni264 – 2663Combined sources
Beta strandi269 – 2735Combined sources
Beta strandi275 – 2773Combined sources
Beta strandi281 – 2833Combined sources
Helixi286 – 2916Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1J8MX-ray2.00F1-292[»]
1J8YX-ray2.00F1-292[»]
ProteinModelPortaliP70722.
SMRiP70722. Positions 3-433.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP70722.

Family & Domainsi

Domaini

Composed of three domains: the N-terminal N domain, which is responsible for interactions with the ribosome, the central G domain, which binds GTP, and the C-terminal M domain, which binds the RNA and the signal sequence of the RNC.UniRule annotation

Sequence similaritiesi

Belongs to the GTP-binding SRP family. SRP54 subfamily.UniRule annotation

Family and domain databases

Gene3Di1.10.260.30. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00306. SRP54.
InterProiIPR003593. AAA+_ATPase.
IPR027417. P-loop_NTPase.
IPR013822. Signal_recog_particl_SRP54_hlx.
IPR004125. Signal_recog_particle_SRP54_M.
IPR022941. SRP54.
IPR000897. SRP54_GTPase_dom.
[Graphical view]
PfamiPF00448. SRP54. 1 hit.
PF02881. SRP54_N. 1 hit.
PF02978. SRP_SPB. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00962. SRP54. 1 hit.
SM00963. SRP54_N. 1 hit.
[Graphical view]
SUPFAMiSSF47364. SSF47364. 1 hit.
SSF47446. SSF47446. 1 hit.
SSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Fragment.

P70722-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
SKLLDNLRDA VRKFLTGSSS YDKAVEDFIK ELQKSLISAD VNVKLVFSLT
60 70 80 90 100
NKIKERLKNE KPPTYIERRE WFIKIVYDEL SNLFGGDKEP KVIPDKIPYV
110 120 130 140 150
IMLVGVQGTG KTTTAGKLAY FYKKKGFKVG LVGADVYRPA ALEQLQQLGQ
160 170 180 190 200
QIGVPVYGEP GEKDAVGIAK RGVEKFLSEK MEIIIVDTAG RHGYGEEAAL
210 220 230 240 250
LEEMKNIYEA IKPDEVTLVI DASIGQKAYD LASKFNQASK IGTIIITKMD
260 270 280 290 300
GTAKGGGALS AVAATGATIK FIGTGEKIDE LEVFNPRRFV ARILGMGDIE
310 320 330 340 350
TILEKIKEVE NYDKMQKKME EVISGKGKLT LRDVYNQLIA LRKMGPLSKL
360 370 380 390 400
FQLLPGIGML GQIPEDQLKV GEEKMRKWLA IMNSMTYEEL DNPSIIDKSR
410 420 430 440 450
MRRIALGSGT EIEDVKELIE HYNLMQRTIK MLKRRKKDVE KLLGQFGGES

T
Length:451
Mass (Da):50,381
Last modified:January 1, 1999 - v2
Checksum:iEAA6F2D901748CEA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y08735 Genomic DNA. Translation: CAA69991.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y08735 Genomic DNA. Translation: CAA69991.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1J8MX-ray2.00F1-292[»]
1J8YX-ray2.00F1-292[»]
ProteinModelPortaliP70722.
SMRiP70722. Positions 3-433.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP70722.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP70722.

Family and domain databases

Gene3Di1.10.260.30. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00306. SRP54.
InterProiIPR003593. AAA+_ATPase.
IPR027417. P-loop_NTPase.
IPR013822. Signal_recog_particl_SRP54_hlx.
IPR004125. Signal_recog_particle_SRP54_M.
IPR022941. SRP54.
IPR000897. SRP54_GTPase_dom.
[Graphical view]
PfamiPF00448. SRP54. 1 hit.
PF02881. SRP54_N. 1 hit.
PF02978. SRP_SPB. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00962. SRP54. 1 hit.
SM00963. SRP54_N. 1 hit.
[Graphical view]
SUPFAMiSSF47364. SSF47364. 1 hit.
SSF47446. SSF47446. 1 hit.
SSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Domain structure, GTP-hydrolyzing activity and 7S RNA binding of Acidianus ambivalens ffh-homologous protein suggest an SRP-like complex in archaea."
    Moll R., Schaefer G., Schmidtke S.
    Eur. J. Biochem. 259:441-448(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Lei 10 / DSM 3772 / JCM 9191.

Entry informationi

Entry nameiSRP54_ACIAM
AccessioniPrimary (citable) accession number: P70722
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: January 1, 1999
Last modified: June 8, 2016
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.