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Protein

Kynureninase

Gene

Kynu

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3-hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3-hydroxyanthranilic acid (3-OHAA), respectively. Has a preference for the L-3-hydroxy form. Also has cysteine-conjugate-beta-lyase activity.UniRule annotation1 Publication

Catalytic activityi

L-kynurenine + H2O = anthranilate + L-alanine.UniRule annotation1 Publication
L-3-hydroxykynurenine + H2O = 3-hydroxyanthranilate + L-alanine.UniRule annotation1 Publication

Cofactori

Enzyme regulationi

Inhibited by o-methylbenzoylalanine (OMBA).1 Publication

Kineticsi

  1. KM=440 µM for L-kynurenine1 Publication
  2. KM=32 µM for DL-3-hydroxykynurenine1 Publication

    pH dependencei

    Optimum pH is about 9.0 with L-kynurenine as substrate, and about 8.5 with DL-3-hydroxykynurenine as substrate.1 Publication

    Pathwayi: L-kynurenine degradation

    This protein is involved in step 1 of the subpathway that synthesizes L-alanine and anthranilate from L-kynurenine.UniRule annotation
    Proteins known to be involved in this subpathway in this organism are:
    1. Kynureninase (Kynu)
    This subpathway is part of the pathway L-kynurenine degradation, which is itself part of Amino-acid degradation.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-alanine and anthranilate from L-kynurenine, the pathway L-kynurenine degradation and in Amino-acid degradation.

    Pathwayi: NAD(+) biosynthesis

    This protein is involved in step 2 of the subpathway that synthesizes quinolinate from L-kynurenine.UniRule annotation
    Proteins known to be involved in the 3 steps of the subpathway in this organism are:
    1. Kynurenine 3-monooxygenase (Kmo)
    2. Kynureninase (Kynu)
    3. 3-hydroxyanthranilate 3,4-dioxygenase (Haao), 3-hydroxyanthranilate 3,4-dioxygenase (Haao)
    This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes quinolinate from L-kynurenine, the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei137Pyridoxal phosphate; via amide nitrogenUniRule annotation1
    Binding sitei138Pyridoxal phosphateUniRule annotation1
    Binding sitei221Pyridoxal phosphateUniRule annotation1
    Binding sitei250Pyridoxal phosphateUniRule annotation1
    Binding sitei253Pyridoxal phosphateUniRule annotation1
    Binding sitei275Pyridoxal phosphateUniRule annotation1
    Binding sitei305Pyridoxal phosphateUniRule annotation1
    Binding sitei333Pyridoxal phosphateUniRule annotation1

    GO - Molecular functioni

    • 3-hydroxykynureninase activity Source: UniProtKB
    • kynureninase activity Source: RGD
    • protein homodimerization activity Source: Ensembl
    • pyridoxal phosphate binding Source: RGD

    GO - Biological processi

    Keywordsi

    Molecular functionHydrolase
    Biological processPyridine nucleotide biosynthesis
    LigandPyridoxal phosphate

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-15007
    ReactomeiR-RNO-71240 Tryptophan catabolism
    SABIO-RKiP70712
    UniPathwayiUPA00253; UER00329
    UPA00334; UER00455

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    KynureninaseUniRule annotation (EC:3.7.1.3UniRule annotation)
    Alternative name(s):
    L-kynurenine hydrolaseUniRule annotation
    Gene namesi
    Name:Kynu
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
    Proteomesi
    • UP000002494 Componenti: Chromosome 3

    Organism-specific databases

    RGDi71061 Kynu

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Chemistry databases

    ChEMBLiCHEMBL2969

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00002186591 – 464KynureninaseAdd BLAST464

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei1N-acetylmethionineUniRule annotation1 Publication1
    Modified residuei276N6-(pyridoxal phosphate)lysineUniRule annotation1

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiP70712
    PRIDEiP70712

    PTM databases

    iPTMnetiP70712
    PhosphoSitePlusiP70712

    Expressioni

    Tissue specificityi

    High levels in liver and kidney. Also detected in heart, retina, ovary. Lung, testis and brain.1 Publication

    Inductioni

    Inhibited by thiol reagents and heavy metal ions.

    Gene expression databases

    BgeeiENSRNOG00000029993
    GenevisibleiP70712 RN

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    GO - Molecular functioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000050947

    Chemistry databases

    BindingDBiP70712

    Structurei

    3D structure databases

    ProteinModelPortaliP70712
    SMRiP70712
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni165 – 168Pyridoxal phosphate bindingUniRule annotation4

    Sequence similaritiesi

    Belongs to the kynureninase family.UniRule annotation

    Phylogenomic databases

    eggNOGiKOG3846 Eukaryota
    COG3844 LUCA
    GeneTreeiENSGT00390000008033
    HOGENOMiHOG000242438
    HOVERGENiHBG001170
    InParanoidiP70712
    KOiK01556
    OMAiVCSLHAS
    OrthoDBiEOG091G077R
    PhylomeDBiP70712

    Family and domain databases

    Gene3Di3.40.640.10, 1 hit
    3.90.1150.10, 2 hits
    HAMAPiMF_01970 Kynureninase, 1 hit
    InterProiView protein in InterPro
    IPR000192 Aminotrans_V_dom
    IPR010111 Kynureninase
    IPR015424 PyrdxlP-dep_Trfase
    IPR015422 PyrdxlP-dep_Trfase_dom1
    IPR015421 PyrdxlP-dep_Trfase_major
    PANTHERiPTHR14084 PTHR14084, 1 hit
    PfamiView protein in Pfam
    PF00266 Aminotran_5, 1 hit
    PIRSFiPIRSF038800 KYNU, 1 hit
    SUPFAMiSSF53383 SSF53383, 1 hit
    TIGRFAMsiTIGR01814 kynureninase, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P70712-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MEPSPLELPV DAVRRIATEL NCDPTDERVA LRLDEEDKLK RFKDCFYIPK
    60 70 80 90 100
    MRDLPSIDLS LVNEDDNAIY FLGNSLGLQP KMVKTYLEEE LDKWAKIGAY
    110 120 130 140 150
    GHEVGKRPWI IGDESIVSLM KDIVGAHEKE IALMNALTVN LHLLLLSFFK
    160 170 180 190 200
    PTPKRHKILL EAKAFPSDHY AIESQIQLHG LDVEKSMRMI KPREGEETLR
    210 220 230 240 250
    MEDILEVIEK EGDSIAVVLF SGLHFYTGQL FNIPAITQAG HAKGCFVGFD
    260 270 280 290 300
    LAHAVGNVEL HLHDWDVDFA CWCSYKYLNS GAGGLAGAFI HEKHAHTIKP
    310 320 330 340 350
    ALVGWFGHEL STRFNMDNKL QLIPGVNGFR ISNPPILLVC SLHASLEIFQ
    360 370 380 390 400
    QATMTALRRK SILLTGYLEY LLKHYHGGND TENKRPVVNI ITPSRAEERG
    410 420 430 440 450
    CQLTLTFSIS KKGVFKELEK RGVVCDKREP EGIRVAPVPL YNSFHDVYKF
    460
    IRLLTAILDS TERN
    Length:464
    Mass (Da):52,470
    Last modified:November 9, 2004 - v2
    Checksum:i1490A74EFF7287AC
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti18T → A in AAC53206 (PubMed:9180257).Curated1
    Sequence conflicti26D → N AA sequence (PubMed:7578221).Curated1
    Sequence conflicti118S → T in AAC53206 (PubMed:9180257).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U68168 mRNA Translation: AAC53206.1
    BC078762 mRNA Translation: AAH78762.1
    PIRiPS0370
    S59898
    T48675
    RefSeqiNP_446354.1, NM_053902.2
    UniGeneiRn.10575

    Genome annotation databases

    EnsembliENSRNOT00000043533; ENSRNOP00000050947; ENSRNOG00000029993
    GeneIDi116682
    KEGGirno:116682
    UCSCiRGD:71061 rat

    Similar proteinsi

    Entry informationi

    Entry nameiKYNU_RAT
    AccessioniPrimary (citable) accession number: P70712
    Secondary accession number(s): Q68G25, Q7M0D0, Q9QW90
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: November 9, 2004
    Last modified: May 23, 2018
    This is version 141 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

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