Reviewed, UniProtKB/Swiss-Prot P70711 (U2D2B_RAT)

Names and origin

Protein names

Recommended name:
    Ubiquitin-conjugating enzyme E2 D2B
    EC=6.3.2.19
Alternative name(s):
    Ubiquitin-protein ligase D2B
    Ubiquitin carrier protein D2B
    Ubiquitin-conjugating enzyme E2-17 kDa 2B
    E2(17)KB 2B

Gene names

Name:	Ube2d2b
Synonyms:	Ube2d2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	147 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the covalent attachment of ubiquitin to other proteins. Mediates the selective degradation of short-lived and abnormal proteins By similarity.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	modification-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW post-translational protein modification Inferred from electronic annotation. Source: InterPro regulation of protein metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 147	147	Ubiquitin-conjugating enzyme E2 D2B		PRO_0000082469
Sites
	Active site	85	1	Glycyl thioester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P70711-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: E950720AEC2AB2B2	FASTA	147	16,659
	10 20 30 40 50 60 MALKRIHKEL NDLAQDPPAQ CSAGPVGEDM FHWQATIMGP NDSPYQGGAF FLTIDFPTEY 70 80 90 100 110 120 PFKPPKVEFT TRIYHPNVNS NGSICLDILR SQWSPALTIS KVLLSISSLL CDPNPDDPLV 130 140 PEIAQIYKTD RDKYNRTARE WTQKYAM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel rat homolog of the Saccharomyces cerevisiae ubiquitin-conjugating enzymes UBC4 and UBC5 with distinct biochemical features is induced during spermatogenesis." Wing S.S., Bedard N., Morales C., Hingamp P., Trasler J. Mol. Cell. Biol. 16:4064-4072(1996) [PubMed: 8754804] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Testis.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U56407 mRNA. Translation: AAC52942.1. BC078808 mRNA. Translation: AAH78808.1.
IPI	IPI00191519.
RefSeq	NP_112263.1.
UniGene	Rn.113868
3D structure databases
SMR	P70711. Positions 1-147.
ModBase	Search...
Protein-protein interaction databases
STRING	P70711.
Genome annotation databases
Ensembl	ENSRNOT00000049772; ENSRNOP00000039621; ENSRNOG00000034016; Rattus norvegicus. [Genome view]
GeneID	79435.
KEGG	rno:79435.
UCSC	NM_031001. rat.
Organism-specific databases
CTD	79435.
RGD	69425. RGD69425.
Phylogenomic databases
eggNOG	maNOG04214.
HOVERGEN	P70711.
InParanoid	P70711.
OMA	LEMASKR.
OrthoDB	EOG9PCDFB.
PhylomeDB	P70711.
Enzyme and pathway databases
BRENDA	6.3.2.19. 248.
Gene expression databases
ArrayExpress	P70711.
Genevestigator	P70711.
GermOnline	ENSRNOG00000034016. Rattus norvegicus.
Family and domain databases
InterPro	IPR016135. UBQ-conjugat/RWD-like. IPR000608. UBQ-conjugat_E2. [Graphical view]
Gene3D	G3DSA:3.10.110.10. UBQ-conjugat_E2. 1 hit.
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
SMART	SM00212. UBCc. 1 hit. [Graphical view]
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	614807.

Entry information

Entry name

U2D2B_RAT

Accession

Primary (citable) accession number: P70711

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	January 19, 2010
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)