P70711 (U2D2B_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin-conjugating enzyme E2 D2B
EC=6.3.2.19

Alternative name(s):
Ubiquitin carrier protein D2B
Ubiquitin-conjugating enzyme E2(17)KB 2B
Ubiquitin-conjugating enzyme E2-17 kDa 2B
Ubiquitin-protein ligase D2B

Gene names

Name:	Ube2d2b
Synonyms:	Ube2d2

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	147 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the covalent attachment of ubiquitin to other proteins. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and autoubiquitination of STUB1 and TRAF6. Involved in the signal-induced conjugation and subsequent degradation of NFKBIA, FBXW2-mediated GCM1 ubiquitination and degradation, MDM2-dependent degradation of p53/TP53 and the activation of MAVS in the mitochondria by DDX58/RIG-I in response to viral infection Plays a role in early maturation of the testis By similarity.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Interacts with CNOT4 (via RING domain) By similarity.
Tissue specificity	Testis-specific. Mainly expressed in the round spermatids (at protein level). Ref.1
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 147	147	Ubiquitin-conjugating enzyme E2 D2B		PRO_0000082469
Sites
	Active site	85	1	Glycyl thioester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P70711 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: E950720AEC2AB2B2	FASTA	147	16,659
	10 20 30 40 50 60 MALKRIHKEL NDLAQDPPAQ CSAGPVGEDM FHWQATIMGP NDSPYQGGAF FLTIDFPTEY 70 80 90 100 110 120 PFKPPKVEFT TRIYHPNVNS NGSICLDILR SQWSPALTIS KVLLSISSLL CDPNPDDPLV 130 140 PEIAQIYKTD RDKYNRTARE WTQKYAM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel rat homolog of the Saccharomyces cerevisiae ubiquitin-conjugating enzymes UBC4 and UBC5 with distinct biochemical features is induced during spermatogenesis." Wing S.S., Bedard N., Morales C., Hingamp P., Trasler J. Mol. Cell. Biol. 16:4064-4072(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Testis.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U56407 mRNA. Translation: AAC52942.1. BC078808 mRNA. Translation: AAH78808.1.
IPI	IPI00191519.
RefSeq	NP_112263.1. NM_031001.2.
UniGene	Rn.113868.
3D structure databases
ProteinModelPortal	P70711.
SMR	P70711. Positions 1-147.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000039621.
PTM databases
PhosphoSite	P70711.
Proteomic databases
PaxDb	P70711.
PRIDE	P70711.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000049772; ENSRNOP00000039621; ENSRNOG00000034016.
GeneID	79435.
KEGG	rno:79435.
UCSC	RGD:69425. rat.
Organism-specific databases
CTD	51619.
RGD	69425. RGD69425.
Phylogenomic databases
eggNOG	COG5078.
GeneTree	ENSGT00700000104033.
HOGENOM	HOG000233455.
HOVERGEN	HBG063308.
InParanoid	P70711.
KO	K06689.
OMA	MIAREWT.
OrthoDB	EOG43BMQX.
Enzyme and pathway databases
UniPathway	UPA00143.
Gene expression databases
Genevestigator	P70711.
GermOnline	ENSRNOG00000034016. Rattus norvegicus.
Family and domain databases
Gene3D	3.10.110.10. 1 hit.
InterPro	IPR000608. UBQ-conjugat_E2. IPR023313. UBQ-conjugating_AS. IPR016135. UBQ-conjugating_enzyme/RWD. [Graphical view]
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
SUPFAM	SSF54495. UBQ-conjugat/RWD-like. 1 hit.
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	614807.

Entry information

Entry name

U2D2B_RAT

Accession

Primary (citable) accession number: P70711

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	April 3, 2013
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program