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P70711 (U2D2B_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin-conjugating enzyme E2 D2B

EC=6.3.2.19
Alternative name(s):
Ubiquitin carrier protein D2B
Ubiquitin-conjugating enzyme E2(17)KB 2B
Ubiquitin-conjugating enzyme E2-17 kDa 2B
Ubiquitin-protein ligase D2B
Gene names
Name:Ube2d2b
Synonyms:Ube2d2
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length147 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the covalent attachment of ubiquitin to other proteins. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and autoubiquitination of STUB1 and TRAF6. Involved in the signal-induced conjugation and subsequent degradation of NFKBIA, FBXW2-mediated GCM1 ubiquitination and degradation, MDM2-dependent degradation of p53/TP53 and the activation of MAVS in the mitochondria by DDX58/RIG-I in response to viral infection Plays a role in early maturation of the testis By similarity.

Catalytic activity

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.

Pathway

Protein modification; protein ubiquitination.

Subunit structure

Interacts with CNOT4 (via RING domain) By similarity.

Tissue specificity

Testis-specific. Mainly expressed in the round spermatids (at protein level). Ref.1

Sequence similarities

Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
   Biological processUbl conjugation pathway
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

ubiquitin-protein ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 147147Ubiquitin-conjugating enzyme E2 D2B
PRO_0000082469

Sites

Active site851Glycyl thioester intermediate By similarity

Sequences

Sequence LengthMass (Da)Tools
P70711 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: E950720AEC2AB2B2

FASTA14716,659
        10         20         30         40         50         60 
MALKRIHKEL NDLAQDPPAQ CSAGPVGEDM FHWQATIMGP NDSPYQGGAF FLTIDFPTEY 

        70         80         90        100        110        120 
PFKPPKVEFT TRIYHPNVNS NGSICLDILR SQWSPALTIS KVLLSISSLL CDPNPDDPLV 

       130        140 
PEIAQIYKTD RDKYNRTARE WTQKYAM 

« Hide

References

« Hide 'large scale' references
[1]"A novel rat homolog of the Saccharomyces cerevisiae ubiquitin-conjugating enzymes UBC4 and UBC5 with distinct biochemical features is induced during spermatogenesis."
Wing S.S., Bedard N., Morales C., Hingamp P., Trasler J.
Mol. Cell. Biol. 16:4064-4072(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Tissue: Testis.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U56407 mRNA. Translation: AAC52942.1.
BC078808 mRNA. Translation: AAH78808.1.
RefSeqNP_112263.1. NM_031001.2.
UniGeneRn.113868.

3D structure databases

ProteinModelPortalP70711.
SMRP70711. Positions 1-147.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000039621.

PTM databases

PhosphoSiteP70711.

Proteomic databases

PaxDbP70711.
PRIDEP70711.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000049772; ENSRNOP00000039621; ENSRNOG00000034016.
GeneID79435.
KEGGrno:79435.
UCSCRGD:69425. rat.

Organism-specific databases

CTD51619.
RGD69425. RGD69425.

Phylogenomic databases

eggNOGCOG5078.
GeneTreeENSGT00740000115162.
HOGENOMHOG000233455.
HOVERGENHBG063308.
InParanoidP70711.
KOK06689.
OMAIAHMCKT.
OrthoDBEOG7PCJGX.
PhylomeDBP70711.
TreeFamTF101108.

Enzyme and pathway databases

UniPathwayUPA00143.

Gene expression databases

GenevestigatorP70711.

Family and domain databases

Gene3D3.10.110.10. 1 hit.
InterProIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMSSF54495. SSF54495. 1 hit.
PROSITEPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio614807.
PROP70711.

Entry information

Entry nameU2D2B_RAT
AccessionPrimary (citable) accession number: P70711
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: April 16, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways