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P70711

- U2D2B_RAT

UniProt

P70711 - U2D2B_RAT

Protein

Ubiquitin-conjugating enzyme E2 D2B

Gene

Ube2d2b

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Catalyzes the covalent attachment of ubiquitin to other proteins. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and autoubiquitination of STUB1 and TRAF6. Involved in the signal-induced conjugation and subsequent degradation of NFKBIA, FBXW2-mediated GCM1 ubiquitination and degradation, MDM2-dependent degradation of p53/TP53 and the activation of MAVS in the mitochondria by DDX58/RIG-I in response to viral infection Plays a role in early maturation of the testis By similarity.PROSITE-ProRule annotation

    Catalytic activityi

    ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei85 – 851Glycyl thioester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. acid-amino acid ligase activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein ubiquitination Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin-conjugating enzyme E2 D2B (EC:6.3.2.19)
    Alternative name(s):
    Ubiquitin carrier protein D2B
    Ubiquitin-conjugating enzyme E2(17)KB 2B
    Ubiquitin-conjugating enzyme E2-17 kDa 2B
    Ubiquitin-protein ligase D2B
    Gene namesi
    Name:Ube2d2b
    Synonyms:Ube2d2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 14

    Organism-specific databases

    RGDi69425. RGD69425.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 147147Ubiquitin-conjugating enzyme E2 D2BPRO_0000082469Add
    BLAST

    Proteomic databases

    PaxDbiP70711.
    PRIDEiP70711.

    PTM databases

    PhosphoSiteiP70711.

    Expressioni

    Tissue specificityi

    Testis-specific. Mainly expressed in the round spermatids (at protein level).1 Publication

    Gene expression databases

    GenevestigatoriP70711.

    Interactioni

    Subunit structurei

    Interacts with CNOT4 (via RING domain).By similarity

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000039621.

    Structurei

    3D structure databases

    ProteinModelPortaliP70711.
    SMRiP70711. Positions 1-147.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5078.
    GeneTreeiENSGT00740000115162.
    HOGENOMiHOG000233455.
    HOVERGENiHBG063308.
    InParanoidiP70711.
    KOiK06689.
    OMAiIYKTDRV.
    OrthoDBiEOG7PCJGX.
    PhylomeDBiP70711.
    TreeFamiTF101108.

    Family and domain databases

    Gene3Di3.10.110.10. 1 hit.
    InterProiIPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view]
    PfamiPF00179. UQ_con. 1 hit.
    [Graphical view]
    SUPFAMiSSF54495. SSF54495. 1 hit.
    PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P70711-1 [UniParc]FASTAAdd to Basket

    « Hide

    MALKRIHKEL NDLAQDPPAQ CSAGPVGEDM FHWQATIMGP NDSPYQGGAF    50
    FLTIDFPTEY PFKPPKVEFT TRIYHPNVNS NGSICLDILR SQWSPALTIS 100
    KVLLSISSLL CDPNPDDPLV PEIAQIYKTD RDKYNRTARE WTQKYAM 147
    Length:147
    Mass (Da):16,659
    Last modified:February 1, 1997 - v1
    Checksum:iE950720AEC2AB2B2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U56407 mRNA. Translation: AAC52942.1.
    BC078808 mRNA. Translation: AAH78808.1.
    RefSeqiNP_112263.1. NM_031001.2.
    UniGeneiRn.113868.

    Genome annotation databases

    EnsembliENSRNOT00000049772; ENSRNOP00000039621; ENSRNOG00000034016.
    GeneIDi79435.
    KEGGirno:79435.
    UCSCiRGD:69425. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U56407 mRNA. Translation: AAC52942.1 .
    BC078808 mRNA. Translation: AAH78808.1 .
    RefSeqi NP_112263.1. NM_031001.2.
    UniGenei Rn.113868.

    3D structure databases

    ProteinModelPortali P70711.
    SMRi P70711. Positions 1-147.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000039621.

    PTM databases

    PhosphoSitei P70711.

    Proteomic databases

    PaxDbi P70711.
    PRIDEi P70711.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000049772 ; ENSRNOP00000039621 ; ENSRNOG00000034016 .
    GeneIDi 79435.
    KEGGi rno:79435.
    UCSCi RGD:69425. rat.

    Organism-specific databases

    CTDi 51619.
    RGDi 69425. RGD69425.

    Phylogenomic databases

    eggNOGi COG5078.
    GeneTreei ENSGT00740000115162.
    HOGENOMi HOG000233455.
    HOVERGENi HBG063308.
    InParanoidi P70711.
    KOi K06689.
    OMAi IYKTDRV.
    OrthoDBi EOG7PCJGX.
    PhylomeDBi P70711.
    TreeFami TF101108.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .

    Miscellaneous databases

    NextBioi 614807.
    PROi P70711.

    Gene expression databases

    Genevestigatori P70711.

    Family and domain databases

    Gene3Di 3.10.110.10. 1 hit.
    InterProi IPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view ]
    Pfami PF00179. UQ_con. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54495. SSF54495. 1 hit.
    PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A novel rat homolog of the Saccharomyces cerevisiae ubiquitin-conjugating enzymes UBC4 and UBC5 with distinct biochemical features is induced during spermatogenesis."
      Wing S.S., Bedard N., Morales C., Hingamp P., Trasler J.
      Mol. Cell. Biol. 16:4064-4072(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Tissue: Testis.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Testis.

    Entry informationi

    Entry nameiU2D2B_RAT
    AccessioniPrimary (citable) accession number: P70711
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 102 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3