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Protein

Ubiquitin-conjugating enzyme E2 D2B

Gene

Ube2d2b

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the covalent attachment of ubiquitin to other proteins. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and autoubiquitination of STUB1 and TRAF6. Involved in the signal-induced conjugation and subsequent degradation of NFKBIA, FBXW2-mediated GCM1 ubiquitination and degradation, MDM2-dependent degradation of p53/TP53 and the activation of MAVS in the mitochondria by DDX58/RIG-I in response to viral infection Plays a role in early maturation of the testis (By similarity).PROSITE-ProRule annotation

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei85 – 851Glycyl thioester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. acid-amino acid ligase activity Source: InterPro
  2. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. protein ubiquitination Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 D2B (EC:6.3.2.19)
Alternative name(s):
Ubiquitin carrier protein D2B
Ubiquitin-conjugating enzyme E2(17)KB 2B
Ubiquitin-conjugating enzyme E2-17 kDa 2B
Ubiquitin-protein ligase D2B
Gene namesi
Name:Ube2d2b
Synonyms:Ube2d2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 14

Organism-specific databases

RGDi69425. RGD69425.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 147147Ubiquitin-conjugating enzyme E2 D2BPRO_0000082469Add
BLAST

Proteomic databases

PaxDbiP70711.
PRIDEiP70711.

PTM databases

PhosphoSiteiP70711.

Expressioni

Tissue specificityi

Testis-specific. Mainly expressed in the round spermatids (at protein level).1 Publication

Gene expression databases

GenevestigatoriP70711.

Interactioni

Subunit structurei

Interacts with CNOT4 (via RING domain).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000039621.

Structurei

3D structure databases

ProteinModelPortaliP70711.
SMRiP70711. Positions 1-147.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5078.
GeneTreeiENSGT00760000119012.
HOGENOMiHOG000233455.
HOVERGENiHBG063308.
InParanoidiP70711.
KOiK06689.
OMAiKELYSVC.
OrthoDBiEOG7PCJGX.
PhylomeDBiP70711.
TreeFamiTF101108.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P70711-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MALKRIHKEL NDLAQDPPAQ CSAGPVGEDM FHWQATIMGP NDSPYQGGAF
60 70 80 90 100
FLTIDFPTEY PFKPPKVEFT TRIYHPNVNS NGSICLDILR SQWSPALTIS
110 120 130 140
KVLLSISSLL CDPNPDDPLV PEIAQIYKTD RDKYNRTARE WTQKYAM
Length:147
Mass (Da):16,659
Last modified:February 1, 1997 - v1
Checksum:iE950720AEC2AB2B2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U56407 mRNA. Translation: AAC52942.1.
BC078808 mRNA. Translation: AAH78808.1.
RefSeqiNP_112263.1. NM_031001.2.
UniGeneiRn.113868.

Genome annotation databases

EnsembliENSRNOT00000049772; ENSRNOP00000039621; ENSRNOG00000034016.
GeneIDi79435.
KEGGirno:79435.
UCSCiRGD:69425. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U56407 mRNA. Translation: AAC52942.1.
BC078808 mRNA. Translation: AAH78808.1.
RefSeqiNP_112263.1. NM_031001.2.
UniGeneiRn.113868.

3D structure databases

ProteinModelPortaliP70711.
SMRiP70711. Positions 1-147.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000039621.

PTM databases

PhosphoSiteiP70711.

Proteomic databases

PaxDbiP70711.
PRIDEiP70711.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000049772; ENSRNOP00000039621; ENSRNOG00000034016.
GeneIDi79435.
KEGGirno:79435.
UCSCiRGD:69425. rat.

Organism-specific databases

CTDi51619.
RGDi69425. RGD69425.

Phylogenomic databases

eggNOGiCOG5078.
GeneTreeiENSGT00760000119012.
HOGENOMiHOG000233455.
HOVERGENiHBG063308.
InParanoidiP70711.
KOiK06689.
OMAiKELYSVC.
OrthoDBiEOG7PCJGX.
PhylomeDBiP70711.
TreeFamiTF101108.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

NextBioi614807.
PROiP70711.

Gene expression databases

GenevestigatoriP70711.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A novel rat homolog of the Saccharomyces cerevisiae ubiquitin-conjugating enzymes UBC4 and UBC5 with distinct biochemical features is induced during spermatogenesis."
    Wing S.S., Bedard N., Morales C., Hingamp P., Trasler J.
    Mol. Cell. Biol. 16:4064-4072(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Testis.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.

Entry informationi

Entry nameiU2D2B_RAT
AccessioniPrimary (citable) accession number: P70711
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: January 7, 2015
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.