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Protein

Eosinophil cationic protein

Gene

Rnase3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Cytotoxin and helminthotoxin with ribonuclease activity. Possesses a wide variety of biological activities (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei38 – 381Proton acceptorBy similarity
Active sitei150 – 1501Proton donorBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Names & Taxonomyi

Protein namesi
Recommended name:
Eosinophil cationic protein (EC:3.1.27.-)
Short name:
ECP
Alternative name(s):
Eosinophil secondary granule ribonuclease 11
Short name:
EAR-11
Ribonuclease 3
Short name:
RNase 3
Gene namesi
Name:Rnase3
Synonyms:Ear11, Rns3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 15

Organism-specific databases

RGDi621580. Rnase3.

Subcellular locationi

  • Cytoplasmic granule By similarity

  • Note: Matrix of eosinophil's large specific granule.By similarity

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence analysisAdd
BLAST
Chaini26 – 155130Eosinophil cationic proteinPRO_0000030866Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi47 ↔ 106By similarity
Disulfide bondi61 ↔ 118By similarity
Disulfide bondi79 ↔ 133By similarity
Disulfide bondi86 ↔ 94By similarity
Glycosylationi88 – 881N-linked (GlcNAc...)Sequence analysis
Glycosylationi107 – 1071N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP70709.
PRIDEiP70709.

Expressioni

Gene expression databases

ExpressionAtlasiP70709. baseline and differential.
GenevisibleiP70709. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000066375.

Structurei

3D structure databases

ProteinModelPortaliP70709.
SMRiP70709. Positions 26-155.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni62 – 665Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the pancreatic ribonuclease family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410JDT3. Eukaryota.
ENOG411136R. LUCA.
HOGENOMiHOG000276882.
HOVERGENiHBG008396.
InParanoidiP70709.
KOiK10787.
OMAiKNCHASS.
OrthoDBiEOG7KDFCP.
PhylomeDBiP70709.
TreeFamiTF333393.

Family and domain databases

Gene3Di3.10.130.10. 1 hit.
InterProiIPR001427. RNaseA.
IPR023411. RNaseA_AS.
IPR023412. RNaseA_domain.
[Graphical view]
PANTHERiPTHR11437. PTHR11437. 1 hit.
PfamiPF00074. RnaseA. 1 hit.
[Graphical view]
PRINTSiPR00794. RIBONUCLEASE.
ProDomiPD000535. RNaseA. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00092. RNAse_Pc. 1 hit.
[Graphical view]
SUPFAMiSSF54076. SSF54076. 1 hit.
PROSITEiPS00127. RNASE_PANCREATIC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P70709-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLKLLESRL CLLLSLGLVL MLASCQPPTP SQWFEIQHIY NRAYPRCNDA
60 70 80 90 100
MRHRNRFTGH CKDINTFLHT SFASVVGVCG NRNIPCGNRT YRNCHNSRYR
110 120 130 140 150
VSITFCNLTT PARIYTQCRY QTTRSRKFYT VGCDPRTPRD SPMYPVVPVH

LDRIF
Length:155
Mass (Da):18,007
Last modified:February 1, 1997 - v1
Checksum:iF190864C34EA22C7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D88586 mRNA. Translation: BAA13648.1.
RefSeqiNP_620257.1. NM_138902.1.
XP_002728238.1. XM_002728192.3.
UniGeneiRn.10626.

Genome annotation databases

EnsembliENSRNOT00000040857; ENSRNOP00000044390; ENSRNOG00000031445.
ENSRNOT00000086535; ENSRNOP00000072837; ENSRNOG00000053457.
GeneIDi100361866.
192264.
KEGGirno:100361866.
rno:192264.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D88586 mRNA. Translation: BAA13648.1.
RefSeqiNP_620257.1. NM_138902.1.
XP_002728238.1. XM_002728192.3.
UniGeneiRn.10626.

3D structure databases

ProteinModelPortaliP70709.
SMRiP70709. Positions 26-155.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000066375.

Proteomic databases

PaxDbiP70709.
PRIDEiP70709.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000040857; ENSRNOP00000044390; ENSRNOG00000031445.
ENSRNOT00000086535; ENSRNOP00000072837; ENSRNOG00000053457.
GeneIDi100361866.
192264.
KEGGirno:100361866.
rno:192264.

Organism-specific databases

CTDi192264.
RGDi621580. Rnase3.

Phylogenomic databases

eggNOGiENOG410JDT3. Eukaryota.
ENOG411136R. LUCA.
HOGENOMiHOG000276882.
HOVERGENiHBG008396.
InParanoidiP70709.
KOiK10787.
OMAiKNCHASS.
OrthoDBiEOG7KDFCP.
PhylomeDBiP70709.
TreeFamiTF333393.

Miscellaneous databases

PROiP70709.

Gene expression databases

ExpressionAtlasiP70709. baseline and differential.
GenevisibleiP70709. RN.

Family and domain databases

Gene3Di3.10.130.10. 1 hit.
InterProiIPR001427. RNaseA.
IPR023411. RNaseA_AS.
IPR023412. RNaseA_domain.
[Graphical view]
PANTHERiPTHR11437. PTHR11437. 1 hit.
PfamiPF00074. RnaseA. 1 hit.
[Graphical view]
PRINTSiPR00794. RIBONUCLEASE.
ProDomiPD000535. RNaseA. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00092. RNAse_Pc. 1 hit.
[Graphical view]
SUPFAMiSSF54076. SSF54076. 1 hit.
PROSITEiPS00127. RNASE_PANCREATIC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Identification of cDNA encoding rat eosinophil cationic protein/eosinophil-associated ribonuclease."
    Nittoh T., Hirakata M., Mue S., Ohuchi K.
    Biochim. Biophys. Acta 1351:42-46(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Bone marrow.

Entry informationi

Entry nameiECP_RAT
AccessioniPrimary (citable) accession number: P70709
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: June 8, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.