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Protein

DNA-directed RNA polymerase I subunit RPA2

Gene

Polr1b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol I is composed of mobile elements and RPA2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft (By similarity).By similarity

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1070 – 1101C4-typeSequence analysisAdd BLAST32

GO - Molecular functioni

GO - Biological processi

  • chromatin silencing at rDNA Source: Reactome
  • embryo implantation Source: MGI
  • nucleologenesis Source: MGI
  • rRNA transcription Source: MGI
  • transcription by RNA polymerase I Source: GOC

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
Biological processTranscription
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-5250924 B-WICH complex positively regulates rRNA expression
R-MMU-573389 NoRC negatively regulates rRNA expression
R-MMU-73762 RNA Polymerase I Transcription Initiation
R-MMU-73772 RNA Polymerase I Promoter Escape
R-MMU-73777 RNA Polymerase I Chain Elongation
R-MMU-73863 RNA Polymerase I Transcription Termination

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase I subunit RPA2 (EC:2.7.7.6)
Short name:
RNA polymerase I subunit 2
Alternative name(s):
DNA-directed RNA polymerase I 135 kDa polypeptide
Short name:
RPA135
Gene namesi
Name:Polr1b
Synonyms:Rpa2, Rpo1-2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:108014 Polr1b

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000480731 – 1135DNA-directed RNA polymerase I subunit RPA2Add BLAST1135

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1051PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP70700
MaxQBiP70700
PaxDbiP70700
PeptideAtlasiP70700
PRIDEiP70700

PTM databases

iPTMnetiP70700
PhosphoSitePlusiP70700

Expressioni

Gene expression databases

BgeeiENSMUSG00000027395
CleanExiMM_RPA2
ExpressionAtlasiP70700 baseline and differential
GenevisibleiP70700 MM

Interactioni

Subunit structurei

Component of the RNA polymerase I (Pol I) complex consisting of at least 13 subunits.By similarity

Protein-protein interaction databases

BioGridi202993, 7 interactors
STRINGi10090.ENSMUSP00000099494

Structurei

3D structure databases

ProteinModelPortaliP70700
SMRiP70700
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RNA polymerase beta chain family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1070 – 1101C4-typeSequence analysisAdd BLAST32

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG0216 Eukaryota
COG0085 LUCA
GeneTreeiENSGT00760000119399
HOGENOMiHOG000222963
HOVERGENiHBG017742
InParanoidiP70700
KOiK03002
OMAiAMNIKVK
OrthoDBiEOG091G00TJ
TreeFamiTF103055

Family and domain databases

CDDicd00653 RNA_pol_B_RPB2, 1 hit
Gene3Di2.40.270.10, 3 hits
2.40.50.150, 2 hits
3.90.1110.10, 1 hit
InterProiView protein in InterPro
IPR015712 DNA-dir_RNA_pol_su2
IPR007120 DNA-dir_RNAP_su2_dom
IPR037033 DNA-dir_RNAP_su2_hyb_sf
IPR007121 RNA_pol_bsu_CS
IPR007644 RNA_pol_bsu_protrusion
IPR007642 RNA_pol_Rpb2_2
IPR037034 RNA_pol_Rpb2_2_sf
IPR007645 RNA_pol_Rpb2_3
IPR007641 RNA_pol_Rpb2_7
IPR014724 RNA_pol_RPB2_OB-fold
IPR009674 Rpa2_dom_4
PANTHERiPTHR20856 PTHR20856, 1 hit
PfamiView protein in Pfam
PF06883 RNA_pol_Rpa2_4, 1 hit
PF04563 RNA_pol_Rpb2_1, 1 hit
PF04561 RNA_pol_Rpb2_2, 1 hit
PF04565 RNA_pol_Rpb2_3, 1 hit
PF00562 RNA_pol_Rpb2_6, 1 hit
PF04560 RNA_pol_Rpb2_7, 1 hit
PROSITEiView protein in PROSITE
PS01166 RNA_POL_BETA, 1 hit

Sequencei

Sequence statusi: Complete.

P70700-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDVDGRWRNL PSGPSLKHLT DPSYGIPPEQ QKAALQDLTR AHVDSFNYAA
60 70 80 90 100
LEGLSHAVQA IPPFEFAFKD ERISLTIVDA VISPPSVPKG TICKDLNVYP
110 120 130 140 150
AECRGRKSTY RGRLTADISW AVNGVPKGII KQFLGYVPIM VKSKLCNLYN
160 170 180 190 200
LPPRVLIEHH EEAEEMGGYF IINGIEKVIR MLIVPRRNFP IAMVRPKWKS
210 220 230 240 250
RGLGYTQFGV SMRCVREEHS AVNMNLHYVE NGTVMLNFIY RKELFFLPLG
260 270 280 290 300
FALKALVSFS DYQIFQELIK GKEEDSFFRN SVSQMLRIVI EEGCHSQKQV
310 320 330 340 350
LNYLGECFRV KLSLPDWYPN VEAAEFLLNQ CICIHLQSNT DKFYLLCLMT
360 370 380 390 400
RKLFALARGE CMDDNPDSLV NQEVLSPGQL FLMFLKEKME NWLVSIKIVL
410 420 430 440 450
DKRAQKANVS INNENLMKIF SMGTELTRPF EYLLATGNLR SKTGLGFLQD
460 470 480 490 500
SGLCVVADKL NFLRYLSHFR CVHRGAAFAK MRTTTVRRLL PESWGFLCPV
510 520 530 540 550
HTPDGAPCGL LNHLTAVCEV VTKFVYTASI PALLCGLGVT PVDTAPCRPY
560 570 580 590 600
SDCYPVLLDG VMVGWVDKDL APEVADTLRR FKVLREKRIP PWMEVALIPM
610 620 630 640 650
TGKPSLYPGL FLFTTPCRLV RPVQNLELGR EELIGTMEQL FMNVAIFEDE
660 670 680 690 700
VFGGISTHQE LFPHSLLSVI ANFIPFSDHN QSPRNMYQCQ MGKQTMGFPL
710 720 730 740 750
LTYQNRSDNK LYRLQTPQSP LVRPCMYDFY DMDNYPIGTN AIVAVISYTG
760 770 780 790 800
YDMEDAMIVN KASWERGFAH GSVYKSEFID LSEKFKQGED NLVFGVKPGD
810 820 830 840 850
PRVMQKLDDD GLPFIGAKLE YGDPYYSYLN LNTGEGFVVY YKSKENCVVD
860 870 880 890 900
NIKVCSNDMG SGKFKCICIT VRIPRNPTIG DKFASRHGQK GILSRLWPAE
910 920 930 940 950
DMPFTESGMM PDILFNPHGF PSRMTIGMLI ESMAGKSAAL HGLCHDATPF
960 970 980 990 1000
IFSEENSALE YFGEMLKAAG YNFYGTERLY SGISGMELEA DIFIGVVYYQ
1010 1020 1030 1040 1050
RLRHMVSDKF QVRTTGARDK VTNQPLGGRN VQGGIRFGEM ERDALLAHGT
1060 1070 1080 1090 1100
SFLLHDRLFN CSDRSVAHMC VECGSLLSPL LEKPPPSWSA MRNRKYNCTV
1110 1120 1130
CGRSDTIDTV SVPYVFRYFV AELAAMNIKV KLDVI
Length:1,135
Mass (Da):128,213
Last modified:July 27, 2011 - v2
Checksum:iAF0E1BE0A98116E6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti184V → E in AAC52850 (PubMed:8921381).Curated1
Sequence conflicti191I → V in AAC52850 (PubMed:8921381).Curated1
Sequence conflicti331C → G in AAC52850 (PubMed:8921381).Curated1
Sequence conflicti346L → R in AAC52850 (PubMed:8921381).Curated1
Sequence conflicti449Q → E in AAC52850 (PubMed:8921381).Curated1
Sequence conflicti525 – 526VY → GD in AAC52850 (PubMed:8921381).Curated2
Sequence conflicti541 – 542PV → GA in AAC52850 (PubMed:8921381).Curated2
Sequence conflicti814F → S in AAC52850 (PubMed:8921381).Curated1
Sequence conflicti1069M → V in AAC52850 (PubMed:8921381).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U58280 mRNA Translation: AAC52850.1
AK143501 mRNA Translation: BAE25401.1
AK144616 mRNA Translation: BAE25971.1
AL833780, BX000699 Genomic DNA Translation: CAM18667.1
BX000699, AL833780 Genomic DNA Translation: CAM27884.1
CH466519 Genomic DNA Translation: EDL28227.1
CCDSiCCDS16720.1
PIRiT42723
RefSeqiNP_033112.2, NM_009086.2
UniGeneiMm.259078

Genome annotation databases

EnsembliENSMUST00000103205; ENSMUSP00000099494; ENSMUSG00000027395
GeneIDi20017
KEGGimmu:20017
UCSCiuc008mhf.2 mouse

Similar proteinsi

Entry informationi

Entry nameiRPA2_MOUSE
AccessioniPrimary (citable) accession number: P70700
Secondary accession number(s): Q3UMX7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 27, 2011
Last modified: March 28, 2018
This is version 141 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health