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Protein

DNA-directed RNA polymerase I subunit RPA2

Gene

Polr1b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol I is composed of mobile elements and RPA2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft (By similarity).By similarity

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1070 – 1101C4-typeSequence analysisAdd BLAST32

GO - Molecular functioni

GO - Biological processi

  • chromatin silencing at rDNA Source: Reactome
  • embryo implantation Source: MGI
  • nucleologenesis Source: MGI
  • rRNA transcription Source: MGI
  • transcription initiation from RNA polymerase I promoter Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Transcription

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-427413. NoRC negatively regulates rRNA expression.
R-MMU-5250924. B-WICH complex positively regulates rRNA expression.
R-MMU-573389. NoRC negatively regulates rRNA expression.
R-MMU-73762. RNA Polymerase I Transcription Initiation.
R-MMU-73772. RNA Polymerase I Promoter Escape.
R-MMU-73777. RNA Polymerase I Chain Elongation.
R-MMU-73863. RNA Polymerase I Transcription Termination.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase I subunit RPA2 (EC:2.7.7.6)
Short name:
RNA polymerase I subunit 2
Alternative name(s):
DNA-directed RNA polymerase I 135 kDa polypeptide
Short name:
RPA135
Gene namesi
Name:Polr1b
Synonyms:Rpa2, Rpo1-2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:108014. Polr1b.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • DNA-directed RNA polymerase I complex Source: MGI
  • nucleolus Source: MGI
  • nucleoplasm Source: Reactome
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000480731 – 1135DNA-directed RNA polymerase I subunit RPA2Add BLAST1135

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1051PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP70700.
MaxQBiP70700.
PaxDbiP70700.
PeptideAtlasiP70700.
PRIDEiP70700.

PTM databases

iPTMnetiP70700.
PhosphoSitePlusiP70700.

Expressioni

Gene expression databases

BgeeiENSMUSG00000027395.
CleanExiMM_RPA2.
ExpressionAtlasiP70700. baseline and differential.
GenevisibleiP70700. MM.

Interactioni

Subunit structurei

Component of the RNA polymerase I (Pol I) complex consisting of at least 13 subunits.By similarity

Protein-protein interaction databases

BioGridi202993. 7 interactors.
STRINGi10090.ENSMUSP00000099494.

Structurei

3D structure databases

ProteinModelPortaliP70700.
SMRiP70700.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RNA polymerase beta chain family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1070 – 1101C4-typeSequence analysisAdd BLAST32

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG0216. Eukaryota.
COG0085. LUCA.
GeneTreeiENSGT00760000119399.
HOGENOMiHOG000222963.
HOVERGENiHBG017742.
InParanoidiP70700.
KOiK03002.
OMAiRMTIAMM.
OrthoDBiEOG091G00TJ.
TreeFamiTF103055.

Family and domain databases

CDDicd00653. RNA_pol_B_RPB2. 1 hit.
Gene3Di2.40.270.10. 2 hits.
2.40.50.150. 1 hit.
3.90.1110.10. 1 hit.
InterProiIPR015712. DNA-dir_RNA_pol_su2.
IPR007120. DNA-dir_RNA_pol_su2_6.
IPR007121. RNA_pol_bsu_CS.
IPR007644. RNA_pol_bsu_protrusion.
IPR007642. RNA_pol_Rpb2_2.
IPR007645. RNA_pol_Rpb2_3.
IPR007641. RNA_pol_Rpb2_7.
IPR014724. RNA_pol_RPB2_OB-fold.
IPR009674. Rpa2_dom_4.
[Graphical view]
PfamiPF06883. RNA_pol_Rpa2_4. 1 hit.
PF04563. RNA_pol_Rpb2_1. 1 hit.
PF04561. RNA_pol_Rpb2_2. 1 hit.
PF04565. RNA_pol_Rpb2_3. 1 hit.
PF00562. RNA_pol_Rpb2_6. 1 hit.
PF04560. RNA_pol_Rpb2_7. 1 hit.
[Graphical view]
PROSITEiPS01166. RNA_POL_BETA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P70700-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDVDGRWRNL PSGPSLKHLT DPSYGIPPEQ QKAALQDLTR AHVDSFNYAA
60 70 80 90 100
LEGLSHAVQA IPPFEFAFKD ERISLTIVDA VISPPSVPKG TICKDLNVYP
110 120 130 140 150
AECRGRKSTY RGRLTADISW AVNGVPKGII KQFLGYVPIM VKSKLCNLYN
160 170 180 190 200
LPPRVLIEHH EEAEEMGGYF IINGIEKVIR MLIVPRRNFP IAMVRPKWKS
210 220 230 240 250
RGLGYTQFGV SMRCVREEHS AVNMNLHYVE NGTVMLNFIY RKELFFLPLG
260 270 280 290 300
FALKALVSFS DYQIFQELIK GKEEDSFFRN SVSQMLRIVI EEGCHSQKQV
310 320 330 340 350
LNYLGECFRV KLSLPDWYPN VEAAEFLLNQ CICIHLQSNT DKFYLLCLMT
360 370 380 390 400
RKLFALARGE CMDDNPDSLV NQEVLSPGQL FLMFLKEKME NWLVSIKIVL
410 420 430 440 450
DKRAQKANVS INNENLMKIF SMGTELTRPF EYLLATGNLR SKTGLGFLQD
460 470 480 490 500
SGLCVVADKL NFLRYLSHFR CVHRGAAFAK MRTTTVRRLL PESWGFLCPV
510 520 530 540 550
HTPDGAPCGL LNHLTAVCEV VTKFVYTASI PALLCGLGVT PVDTAPCRPY
560 570 580 590 600
SDCYPVLLDG VMVGWVDKDL APEVADTLRR FKVLREKRIP PWMEVALIPM
610 620 630 640 650
TGKPSLYPGL FLFTTPCRLV RPVQNLELGR EELIGTMEQL FMNVAIFEDE
660 670 680 690 700
VFGGISTHQE LFPHSLLSVI ANFIPFSDHN QSPRNMYQCQ MGKQTMGFPL
710 720 730 740 750
LTYQNRSDNK LYRLQTPQSP LVRPCMYDFY DMDNYPIGTN AIVAVISYTG
760 770 780 790 800
YDMEDAMIVN KASWERGFAH GSVYKSEFID LSEKFKQGED NLVFGVKPGD
810 820 830 840 850
PRVMQKLDDD GLPFIGAKLE YGDPYYSYLN LNTGEGFVVY YKSKENCVVD
860 870 880 890 900
NIKVCSNDMG SGKFKCICIT VRIPRNPTIG DKFASRHGQK GILSRLWPAE
910 920 930 940 950
DMPFTESGMM PDILFNPHGF PSRMTIGMLI ESMAGKSAAL HGLCHDATPF
960 970 980 990 1000
IFSEENSALE YFGEMLKAAG YNFYGTERLY SGISGMELEA DIFIGVVYYQ
1010 1020 1030 1040 1050
RLRHMVSDKF QVRTTGARDK VTNQPLGGRN VQGGIRFGEM ERDALLAHGT
1060 1070 1080 1090 1100
SFLLHDRLFN CSDRSVAHMC VECGSLLSPL LEKPPPSWSA MRNRKYNCTV
1110 1120 1130
CGRSDTIDTV SVPYVFRYFV AELAAMNIKV KLDVI
Length:1,135
Mass (Da):128,213
Last modified:July 27, 2011 - v2
Checksum:iAF0E1BE0A98116E6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti184V → E in AAC52850 (PubMed:8921381).Curated1
Sequence conflicti191I → V in AAC52850 (PubMed:8921381).Curated1
Sequence conflicti331C → G in AAC52850 (PubMed:8921381).Curated1
Sequence conflicti346L → R in AAC52850 (PubMed:8921381).Curated1
Sequence conflicti449Q → E in AAC52850 (PubMed:8921381).Curated1
Sequence conflicti525 – 526VY → GD in AAC52850 (PubMed:8921381).Curated2
Sequence conflicti541 – 542PV → GA in AAC52850 (PubMed:8921381).Curated2
Sequence conflicti814F → S in AAC52850 (PubMed:8921381).Curated1
Sequence conflicti1069M → V in AAC52850 (PubMed:8921381).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U58280 mRNA. Translation: AAC52850.1.
AK143501 mRNA. Translation: BAE25401.1.
AK144616 mRNA. Translation: BAE25971.1.
AL833780, BX000699 Genomic DNA. Translation: CAM18667.1.
BX000699, AL833780 Genomic DNA. Translation: CAM27884.1.
CH466519 Genomic DNA. Translation: EDL28227.1.
CCDSiCCDS16720.1.
PIRiT42723.
RefSeqiNP_033112.2. NM_009086.2.
UniGeneiMm.259078.

Genome annotation databases

EnsembliENSMUST00000103205; ENSMUSP00000099494; ENSMUSG00000027395.
GeneIDi20017.
KEGGimmu:20017.
UCSCiuc008mhf.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U58280 mRNA. Translation: AAC52850.1.
AK143501 mRNA. Translation: BAE25401.1.
AK144616 mRNA. Translation: BAE25971.1.
AL833780, BX000699 Genomic DNA. Translation: CAM18667.1.
BX000699, AL833780 Genomic DNA. Translation: CAM27884.1.
CH466519 Genomic DNA. Translation: EDL28227.1.
CCDSiCCDS16720.1.
PIRiT42723.
RefSeqiNP_033112.2. NM_009086.2.
UniGeneiMm.259078.

3D structure databases

ProteinModelPortaliP70700.
SMRiP70700.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202993. 7 interactors.
STRINGi10090.ENSMUSP00000099494.

PTM databases

iPTMnetiP70700.
PhosphoSitePlusiP70700.

Proteomic databases

EPDiP70700.
MaxQBiP70700.
PaxDbiP70700.
PeptideAtlasiP70700.
PRIDEiP70700.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000103205; ENSMUSP00000099494; ENSMUSG00000027395.
GeneIDi20017.
KEGGimmu:20017.
UCSCiuc008mhf.2. mouse.

Organism-specific databases

CTDi84172.
MGIiMGI:108014. Polr1b.

Phylogenomic databases

eggNOGiKOG0216. Eukaryota.
COG0085. LUCA.
GeneTreeiENSGT00760000119399.
HOGENOMiHOG000222963.
HOVERGENiHBG017742.
InParanoidiP70700.
KOiK03002.
OMAiRMTIAMM.
OrthoDBiEOG091G00TJ.
TreeFamiTF103055.

Enzyme and pathway databases

ReactomeiR-MMU-427413. NoRC negatively regulates rRNA expression.
R-MMU-5250924. B-WICH complex positively regulates rRNA expression.
R-MMU-573389. NoRC negatively regulates rRNA expression.
R-MMU-73762. RNA Polymerase I Transcription Initiation.
R-MMU-73772. RNA Polymerase I Promoter Escape.
R-MMU-73777. RNA Polymerase I Chain Elongation.
R-MMU-73863. RNA Polymerase I Transcription Termination.

Miscellaneous databases

PROiP70700.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027395.
CleanExiMM_RPA2.
ExpressionAtlasiP70700. baseline and differential.
GenevisibleiP70700. MM.

Family and domain databases

CDDicd00653. RNA_pol_B_RPB2. 1 hit.
Gene3Di2.40.270.10. 2 hits.
2.40.50.150. 1 hit.
3.90.1110.10. 1 hit.
InterProiIPR015712. DNA-dir_RNA_pol_su2.
IPR007120. DNA-dir_RNA_pol_su2_6.
IPR007121. RNA_pol_bsu_CS.
IPR007644. RNA_pol_bsu_protrusion.
IPR007642. RNA_pol_Rpb2_2.
IPR007645. RNA_pol_Rpb2_3.
IPR007641. RNA_pol_Rpb2_7.
IPR014724. RNA_pol_RPB2_OB-fold.
IPR009674. Rpa2_dom_4.
[Graphical view]
PfamiPF06883. RNA_pol_Rpa2_4. 1 hit.
PF04563. RNA_pol_Rpb2_1. 1 hit.
PF04561. RNA_pol_Rpb2_2. 1 hit.
PF04565. RNA_pol_Rpb2_3. 1 hit.
PF00562. RNA_pol_Rpb2_6. 1 hit.
PF04560. RNA_pol_Rpb2_7. 1 hit.
[Graphical view]
PROSITEiPS01166. RNA_POL_BETA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRPA2_MOUSE
AccessioniPrimary (citable) accession number: P70700
Secondary accession number(s): Q3UMX7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.