Reviewed, UniProtKB/Swiss-Prot P70700 (RPA2_MOUSE)

Names and origin

Protein names

Recommended name:
    DNA-directed RNA polymerase I subunit RPA2
      Short name=RNA polymerase I subunit 2
    EC=2.7.7.6
Alternative name(s):
    DNA-directed RNA polymerase I 135 kDa polypeptide
      Short name=RPA135

Gene names

Name:	Polr1b
Synonyms:	Rpa2, Rpo1-2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	1135 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol I is composed of mobile elements and RPA2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Component of the RNA polymerase I (Pol I) complex consisting of at least 13 subunits By similarity.
Subcellular location	Nucleus › nucleolus By similarity.
Sequence similarities	Belongs to the RNA polymerase beta chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase Nucleus
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Nucleotidyltransferase Transferase
Gene Ontology (GO)
Biological process	transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleolus Inferred from direct assay. Source: MGI
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: MGI ribonucleoside binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1135	1135	DNA-directed RNA polymerase I subunit RPA2		PRO_0000048073
Regions
	Zinc finger	1070 – 1101	32	C4-type Potential

Sequences

	Sequence		Length	Mass (Da)
	P70700-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 73CAE4BB877608C1	FASTA	1,135	127,976
	10 20 30 40 50 60 MDVDGRWRNL PSGPSLKHLT DPSYGIPPEQ QKAALQDLTR AHVDSFNYAA LEGLSHAVQA 70 80 90 100 110 120 IPPFEFAFKD ERISLTIVDA VISPPSVPKG TICKDLNVYP AECRGRKSTY RGRLTADISW 130 140 150 160 170 180 AVNGVPKGII KQFLGYVPIM VKSKLCNLYN LPPRVLIEHH EEAEEMGGYF IINGIEKVIR 190 200 210 220 230 240 MLIEPRRNFP VAMVRPKWKS RGLGYTQFGV SMRCVREEHS AVNMNLHYVE NGTVMLNFIY 250 260 270 280 290 300 RKELFFLPLG FALKALVSFS DYQIFQELIK GKEEDSFFRN SVSQMLRIVI EEGCHSQKQV 310 320 330 340 350 360 LNYLGECFRV KLSLPDWYPN VEAAEFLLNQ GICIHLQSNT DKFYLRCLMT RKLFALARGE 370 380 390 400 410 420 CMDDNPDSLV NQEVLSPGQL FLMFLKEKME NWLVSIKIVL DKRAQKANVS INNENLMKIF 430 440 450 460 470 480 SMGTELTRPF EYLLATGNLR SKTGLGFLED SGLCVVADKL NFLRYLSHFR CVHRGAAFAK 490 500 510 520 530 540 MRTTTVRRLL PESWGFLCPV HTPDGAPCGL LNHLTAVCEV VTKFGDTASI PALLCGLGVT 550 560 570 580 590 600 GADTAPCRPY SDCYPVLLDG VMVGWVDKDL APEVADTLRR FKVLREKRIP PWMEVALIPM 610 620 630 640 650 660 TGKPSLYPGL FLFTTPCRLV RPVQNLELGR EELIGTMEQL FMNVAIFEDE VFGGISTHQE 670 680 690 700 710 720 LFPHSLLSVI ANFIPFSDHN QSPRNMYQCQ MGKQTMGFPL LTYQNRSDNK LYRLQTPQSP 730 740 750 760 770 780 LVRPCMYDFY DMDNYPIGTN AIVAVISYTG YDMEDAMIVN KASWERGFAH GSVYKSEFID 790 800 810 820 830 840 LSEKFKQGED NLVFGVKPGD PRVMQKLDDD GLPSIGAKLE YGDPYYSYLN LNTGEGFVVY 850 860 870 880 890 900 YKSKENCVVD NIKVCSNDMG SGKFKCICIT VRIPRNPTIG DKFASRHGQK GILSRLWPAE 910 920 930 940 950 960 DMPFTESGMM PDILFNPHGF PSRMTIGMLI ESMAGKSAAL HGLCHDATPF IFSEENSALE 970 980 990 1000 1010 1020 YFGEMLKAAG YNFYGTERLY SGISGMELEA DIFIGVVYYQ RLRHMVSDKF QVRTTGARDK 1030 1040 1050 1060 1070 1080 VTNQPLGGRN VQGGIRFGEM ERDALLAHGT SFLLHDRLFN CSDRSVAHVC VECGSLLSPL 1090 1100 1110 1120 1130 LEKPPPSWSA MRNRKYNCTV CGRSDTIDTV SVPYVFRYFV AELAAMNIKV KLDVI

References

[1]	"Molecular cloning of RPA2, the gene encoding the second largest subunit of mouse RNA polymerase I." Seither P., Grummt I. Genomics 37:135-139(1996) [PubMed: 8921381] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U58280 mRNA. Translation: AAC52850.1.
IPI	IPI00112769.
PIR	T42723.
UniGene	Mm.259078
3D structure databases
HSSP	HSSP built from PDB template 1I50 based on UniProtKB P08518.
ModBase	Search...
PTM databases
PhosphoSite	P70700.
Proteomic databases
PRIDE	P70700.
Genome annotation databases
Ensembl	ENSMUSG00000027395. Mus musculus. [Contig view]
KEGG	mmu:20017.
Organism-specific databases
MGI	MGI:108014. Rpo1-2.
Phylogenomic databases
HOGENOM	P70700.
HOVERGEN	P70700.
Enzyme and pathway databases
BRENDA	2.7.7.6. 244.
Gene expression databases
ArrayExpress	P70700.
Bgee	P70700.
CleanEx	MM_RPA2.
GermOnline	ENSMUSG00000027395. Mus musculus.
Family and domain databases
InterPro	IPR015712. DNA-dir_RNA_pol_su2. IPR007120. DNA-dir_RNA_pol_su2_6. IPR007121. RNA_pol_bsu_CS. IPR007644. RNA_pol_bsu_protrusion. IPR009674. RNA_pol_Rpa2-specific. IPR007642. RNA_pol_Rpb2_2. IPR007645. RNA_pol_Rpb2_3. IPR007647. RNA_pol_Rpb2_5. IPR007641. RNA_pol_Rpb2_7. [Graphical view]
PANTHER	PTHR20856. RNA_pol_I_sub2. 1 hit.
Pfam	PF06883. RNA_pol_Rpa2_4. 1 hit. PF04563. RNA_pol_Rpb2_1. 1 hit. PF04561. RNA_pol_Rpb2_2. 1 hit. PF04565. RNA_pol_Rpb2_3. 1 hit. PF04567. RNA_pol_Rpb2_5. 1 hit. PF00562. RNA_pol_Rpb2_6. 1 hit. PF04560. RNA_pol_Rpb2_7. 1 hit. [Graphical view]
PROSITE	PS01166. RNA_POL_BETA. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	297521.
SOURCE	Search...

Entry information

Entry name

RPA2_MOUSE

Accession

Primary (citable) accession number: P70700

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	June 16, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)