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P70699 (LYAG_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lysosomal alpha-glucosidase
EC=3.2.1.20
Alternative name(s):
Acid maltase
Gene names
Name:Gaa
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length953 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Essential for the degradation of glygogen to glucose in lysosomes.

Catalytic activity

Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose.

Subcellular location

Lysosome. Lysosome membrane By similarity.

Sequence similarities

Belongs to the glycosyl hydrolase 31 family.

Contains 1 P-type (trefoil) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727 Potential
Propeptide28 – 6942 By similarity
PRO_0000018569
Chain70 – 953884Lysosomal alpha-glucosidase
PRO_0000018570

Regions

Domain80 – 13152P-type

Sites

Active site5181Nucleophile By similarity
Active site5211 By similarity

Amino acid modifications

Glycosylation1401N-linked (GlcNAc...) Ref.5
Glycosylation2331N-linked (GlcNAc...) Potential
Glycosylation3901N-linked (GlcNAc...) Potential
Glycosylation4701N-linked (GlcNAc...) Ref.5
Glycosylation8831N-linked (GlcNAc...) Potential
Glycosylation9261N-linked (GlcNAc...) Potential
Glycosylation9331N-linked (GlcNAc...) Potential
Disulfide bond82 ↔ 109 By similarity
Disulfide bond92 ↔ 108 By similarity
Disulfide bond103 ↔ 127 By similarity

Experimental info

Sequence conflict621K → E in AAB06943. Ref.1
Sequence conflict2541S → A in AAB06943. Ref.1
Sequence conflict430 – 4312EL → DV in AAB06943. Ref.1
Sequence conflict4341D → G Ref.1
Sequence conflict4341D → G Ref.3
Sequence conflict4811W → C in AAB06943. Ref.1
Sequence conflict6151G → E in AAB06943. Ref.1
Sequence conflict6191S → T in AAB06943. Ref.1
Sequence conflict7321P → R in AAB06943. Ref.1
Sequence conflict7771M → V Ref.1
Sequence conflict7771M → V Ref.3
Sequence conflict8711R → H in AAB06943. Ref.1
Sequence conflict9031R → K in AAB06943. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P70699 [UniParc].

Last modified August 16, 2004. Version 2.
Checksum: 956B89685FB5FF81

FASTA953106,248
        10         20         30         40         50         60 
MNIRKPLCSN SVVGACTLIS LTTAVILGHL MLRELMLLPQ DLHESSSGLW KTYRPHHQEG 

        70         80         90        100        110        120 
YKPGPLHIQE QTEQPKEAPT QCDVPPSSRF DCAPDKGISQ EQCEARGCCY VPAGQVLKEP 

       130        140        150        160        170        180 
QIGQPWCFFP PSYPSYRLEN LSSTESGYTA TLTRTSPTFF PKDVLTLQLE VLMETDSRLH 

       190        200        210        220        230        240 
FKIKDPASKR YEVPLETPRV LSQAPSPLYS VEFSEEPFGV IVRRKLGGRV LLNTTVAPLF 

       250        260        270        280        290        300 
FADQFLQLST SLPSQHITGL GEHLSPLMLS TDWARITLWN RDTPPSQGTN LYGSHPFYLA 

       310        320        330        340        350        360 
LEDGGLAHGV FLLNSNAMDV ILQPSPALTW RSTGGILDVY VFLGPEPKSV VQQYLDVVGY 

       370        380        390        400        410        420 
PFMPPYWGLG FHLCRWGYSS TAIVRQVVEN MTRTHFPLDV QWNDLDYMDA RRDFTFNQDS 

       430        440        450        460        470        480 
FADFPDMVRE LHQDGRRYMM IVDPAISSAG PAGSYRPYDE GLRRGVFITN ETGQPLIGKV 

       490        500        510        520        530        540 
WPGTTAFPDF TNPETLDWWQ DMVSEFHAQV PFDGMWLDMN EPSNFVRGSQ QGCPNNELEN 

       550        560        570        580        590        600 
PPYVPGVVGG ILQAATICAS SHQFLSTHYN LHNLYGLTEA IASSRALVKT RGTRPFVISR 

       610        620        630        640        650        660 
STFSGHGRYA GHWTGDVRSS WEHLAYSVPD ILQFNLLGVP LVGADICGFI GDTSEELCVR 

       670        680        690        700        710        720 
WTQLGAFYPF MRNHNDLNSV PQEPYRFSET AQQAMRKAFA LRYALLPYLY TLFHRAHVRG 

       730        740        750        760        770        780 
DTVARPLFLE FPEDPSTWSV DRQLLWGPAL LITPVLEPGK TEVTGYFPKG TWYNMQMVSV 

       790        800        810        820        830        840 
DSLGTLPSPS SASSFRSAVQ SKGQWLTLEA PLDTINVHLR EGYIIPLQGP SLTTTESRKQ 

       850        860        870        880        890        900 
PMALAVALTA SGEADGELFW DDGESLAVLE RGAYTLVTFS AKNNTIVNKL VRVTKEGAEL 

       910        920        930        940        950 
QLREVTVLGV ATAPTQVLSN GIPVSNFTYS PDNKSLAIPV SLLMGELFQI SWS

« Hide

References

« Hide 'large scale' references
[1]"Cloning and characterization of the mouse liver cDNA encoding lysosomal alpha-glucosidase."
Ding J.H., Yang B.Z., Reuser A.J.J., Roe C.R.
Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
Tissue: Liver.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J and NOD.
Tissue: Bone marrow, Brain cortex, Heart and Thymus.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Mammary tumor.
[4]Lubec G., Sunyer B., Chen W.-Q.
Submitted (JAN-2009) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 376-385, MASS SPECTROMETRY.
Strain: OF1.
Tissue: Hippocampus.
[5]"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
Nat. Biotechnol. 27:378-386(2009) [PubMed: 19349973] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-140 AND ASN-470, MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U49351 mRNA. Translation: AAB06943.1.
AK052211 mRNA. Translation: BAC34888.1.
AK088481 mRNA. Translation: BAC40382.1.
AK139333 mRNA. Translation: BAE23960.1.
AK146538 mRNA. Translation: BAE27243.1.
AK150970 mRNA. Translation: BAE30001.1.
BC010210 mRNA. Translation: AAH10210.1.
IPIIPI00111960.
RefSeqNP_001152796.1. NM_001159324.1.
NP_032090.3. NM_008064.3.
UniGeneMm.4793.

3D structure databases

ProteinModelPortalP70699.
SMRP70699. Positions 84-953.
ModBaseSearch...

Protein-protein interaction databases

STRINGP70699.

Protein family/group databases

CAZyGH31. Glycoside Hydrolase Family 31.

PTM databases

PhosphoSiteP70699.

Proteomic databases

PRIDEP70699.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000026666; ENSMUSP00000026666; ENSMUSG00000025579.
ENSMUST00000106259; ENSMUSP00000101866; ENSMUSG00000025579.
GeneID14387.
KEGGmmu:14387.

Organism-specific databases

CTD2548.
MGIMGI:95609. Gaa.

Phylogenomic databases

GeneTreeENSGT00550000074344.
HOGENOMHBG738610.
HOVERGENHBG006297.
InParanoidP70699.
OMATICASSR.
OrthoDBEOG4MKNFR.
PhylomeDBP70699.

Gene expression databases

ArrayExpressP70699.
BgeeP70699.
CleanExMM_GAA.
GenevestigatorP70699.
GermOnlineENSMUSG00000025579. Mus musculus.

Family and domain databases

InterProIPR011013. Glyco_hydro-type_carb-bd.
IPR000322. Glyco_hydro_31.
IPR017853. Glycoside_hydrolase_SF.
IPR000519. P_trefoil.
IPR017957. P_trefoil_CS.
[Graphical view]
Gene3DG3DSA:4.10.110.10. P_trefoil. 1 hit.
KOK12316.
PANTHERPTHR22762. Glyco_hydro_31. 1 hit.
PfamPF01055. Glyco_hydro_31. 1 hit.
PF00088. Trefoil. 1 hit.
[Graphical view]
SMARTSM00018. PD. 1 hit.
[Graphical view]
SUPFAMSSF74650. Gal_mut_like. 1 hit.
SSF51445. Glyco_hydro_cat. 1 hit.
PROSITEPS00129. GLYCOSYL_HYDROL_F31_1. 1 hit.
PS00707. GLYCOSYL_HYDROL_F31_2. 1 hit.
PS00025. P_TREFOIL_1. 1 hit.
PS51448. P_TREFOIL_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio285901.
SOURCESearch...

Entry information

Entry nameLYAG_MOUSE
AccessionPrimary (citable) accession number: P70699
Secondary accession number(s): Q3UJB2, Q8BGI6, Q91Z45
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: August 16, 2004
Last modified: November 16, 2011
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents