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P70699

- LYAG_MOUSE

UniProt

P70699 - LYAG_MOUSE

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Protein

Lysosomal alpha-glucosidase

Gene

Gaa

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Essential for the degradation of glygogen to glucose in lysosomes.

Catalytic activityi

Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei518 – 5181NucleophilePROSITE-ProRule annotation
Active sitei521 – 5211By similarity

GO - Molecular functioni

  1. alpha-1,4-glucosidase activity Source: MGI
  2. carbohydrate binding Source: InterPro
  3. maltose alpha-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. cardiac muscle contraction Source: Ensembl
  2. diaphragm contraction Source: MGI
  3. glycogen catabolic process Source: MGI
  4. glycogen metabolic process Source: MGI
  5. heart morphogenesis Source: MGI
  6. locomotory behavior Source: MGI
  7. lysosome organization Source: MGI
  8. muscle cell cellular homeostasis Source: MGI
  9. neuromuscular process controlling balance Source: MGI
  10. neuromuscular process controlling posture Source: MGI
  11. regulation of the force of heart contraction Source: MGI
  12. striated muscle contraction Source: MGI
  13. tissue development Source: MGI
  14. vacuolar sequestering Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH31. Glycoside Hydrolase Family 31.

Names & Taxonomyi

Protein namesi
Recommended name:
Lysosomal alpha-glucosidase (EC:3.2.1.20)
Alternative name(s):
Acid maltase
Gene namesi
Name:Gaa
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:95609. Gaa.

Subcellular locationi

GO - Cellular componenti

  1. extracellular vesicular exosome Source: Ensembl
  2. lysosomal membrane Source: Ensembl
  3. lysosome Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727Sequence AnalysisAdd
BLAST
Propeptidei28 – 6942By similarityPRO_0000018569Add
BLAST
Chaini70 – 953884Lysosomal alpha-glucosidasePRO_0000018570Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi82 ↔ 109PROSITE-ProRule annotation
Disulfide bondi92 ↔ 108PROSITE-ProRule annotation
Disulfide bondi103 ↔ 127PROSITE-ProRule annotation
Glycosylationi140 – 1401N-linked (GlcNAc...)1 Publication
Glycosylationi233 – 2331N-linked (GlcNAc...)Sequence Analysis
Glycosylationi390 – 3901N-linked (GlcNAc...)Sequence Analysis
Glycosylationi470 – 4701N-linked (GlcNAc...)1 Publication
Glycosylationi883 – 8831N-linked (GlcNAc...)Sequence Analysis
Glycosylationi926 – 9261N-linked (GlcNAc...)Sequence Analysis
Glycosylationi933 – 9331N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP70699.
PaxDbiP70699.
PRIDEiP70699.

PTM databases

PhosphoSiteiP70699.

Expressioni

Gene expression databases

BgeeiP70699.
CleanExiMM_GAA.
ExpressionAtlasiP70699. baseline and differential.
GenevestigatoriP70699.

Interactioni

Protein-protein interaction databases

IntActiP70699. 1 interaction.
MINTiMINT-4100796.

Structurei

3D structure databases

ProteinModelPortaliP70699.
SMRiP70699. Positions 89-953.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini80 – 13152P-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 31 family.Curated
Contains 1 P-type (trefoil) domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1501.
GeneTreeiENSGT00760000119229.
HOGENOMiHOG000041175.
HOVERGENiHBG006297.
InParanoidiP70699.
KOiK12316.
OMAiSSEMGYT.
OrthoDBiEOG77HDD0.
PhylomeDBiP70699.
TreeFamiTF314577.

Family and domain databases

Gene3Di4.10.110.10. 1 hit.
InterProiIPR011013. Gal_mutarotase_SF_dom.
IPR000322. Glyco_hydro_31.
IPR025887. Glyco_hydro_31_N_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR000519. P_trefoil.
IPR017957. P_trefoil_CS.
[Graphical view]
PfamiPF13802. Gal_mutarotas_2. 1 hit.
PF01055. Glyco_hydro_31. 1 hit.
PF00088. Trefoil. 1 hit.
[Graphical view]
SMARTiSM00018. PD. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 2 hits.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00129. GLYCOSYL_HYDROL_F31_1. 1 hit.
PS00707. GLYCOSYL_HYDROL_F31_2. 1 hit.
PS00025. P_TREFOIL_1. 1 hit.
PS51448. P_TREFOIL_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P70699-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNIRKPLCSN SVVGACTLIS LTTAVILGHL MLRELMLLPQ DLHESSSGLW
60 70 80 90 100
KTYRPHHQEG YKPGPLHIQE QTEQPKEAPT QCDVPPSSRF DCAPDKGISQ
110 120 130 140 150
EQCEARGCCY VPAGQVLKEP QIGQPWCFFP PSYPSYRLEN LSSTESGYTA
160 170 180 190 200
TLTRTSPTFF PKDVLTLQLE VLMETDSRLH FKIKDPASKR YEVPLETPRV
210 220 230 240 250
LSQAPSPLYS VEFSEEPFGV IVRRKLGGRV LLNTTVAPLF FADQFLQLST
260 270 280 290 300
SLPSQHITGL GEHLSPLMLS TDWARITLWN RDTPPSQGTN LYGSHPFYLA
310 320 330 340 350
LEDGGLAHGV FLLNSNAMDV ILQPSPALTW RSTGGILDVY VFLGPEPKSV
360 370 380 390 400
VQQYLDVVGY PFMPPYWGLG FHLCRWGYSS TAIVRQVVEN MTRTHFPLDV
410 420 430 440 450
QWNDLDYMDA RRDFTFNQDS FADFPDMVRE LHQDGRRYMM IVDPAISSAG
460 470 480 490 500
PAGSYRPYDE GLRRGVFITN ETGQPLIGKV WPGTTAFPDF TNPETLDWWQ
510 520 530 540 550
DMVSEFHAQV PFDGMWLDMN EPSNFVRGSQ QGCPNNELEN PPYVPGVVGG
560 570 580 590 600
ILQAATICAS SHQFLSTHYN LHNLYGLTEA IASSRALVKT RGTRPFVISR
610 620 630 640 650
STFSGHGRYA GHWTGDVRSS WEHLAYSVPD ILQFNLLGVP LVGADICGFI
660 670 680 690 700
GDTSEELCVR WTQLGAFYPF MRNHNDLNSV PQEPYRFSET AQQAMRKAFA
710 720 730 740 750
LRYALLPYLY TLFHRAHVRG DTVARPLFLE FPEDPSTWSV DRQLLWGPAL
760 770 780 790 800
LITPVLEPGK TEVTGYFPKG TWYNMQMVSV DSLGTLPSPS SASSFRSAVQ
810 820 830 840 850
SKGQWLTLEA PLDTINVHLR EGYIIPLQGP SLTTTESRKQ PMALAVALTA
860 870 880 890 900
SGEADGELFW DDGESLAVLE RGAYTLVTFS AKNNTIVNKL VRVTKEGAEL
910 920 930 940 950
QLREVTVLGV ATAPTQVLSN GIPVSNFTYS PDNKSLAIPV SLLMGELFQI

SWS
Length:953
Mass (Da):106,248
Last modified:August 16, 2004 - v2
Checksum:i956B89685FB5FF81
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti62 – 621K → E in AAB06943. 1 PublicationCurated
Sequence conflicti254 – 2541S → A in AAB06943. 1 PublicationCurated
Sequence conflicti430 – 4312EL → DV in AAB06943. 1 PublicationCurated
Sequence conflicti434 – 4341D → G1 PublicationCurated
Sequence conflicti434 – 4341D → G(PubMed:15489334)Curated
Sequence conflicti481 – 4811W → C in AAB06943. 1 PublicationCurated
Sequence conflicti615 – 6151G → E in AAB06943. 1 PublicationCurated
Sequence conflicti619 – 6191S → T in AAB06943. 1 PublicationCurated
Sequence conflicti732 – 7321P → R in AAB06943. 1 PublicationCurated
Sequence conflicti777 – 7771M → V1 PublicationCurated
Sequence conflicti777 – 7771M → V(PubMed:15489334)Curated
Sequence conflicti871 – 8711R → H in AAB06943. 1 PublicationCurated
Sequence conflicti903 – 9031R → K in AAB06943. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U49351 mRNA. Translation: AAB06943.1.
AK052211 mRNA. Translation: BAC34888.1.
AK088481 mRNA. Translation: BAC40382.1.
AK139333 mRNA. Translation: BAE23960.1.
AK146538 mRNA. Translation: BAE27243.1.
AK150970 mRNA. Translation: BAE30001.1.
BC010210 mRNA. Translation: AAH10210.1.
CCDSiCCDS25713.1.
RefSeqiNP_001152796.1. NM_001159324.1.
NP_032090.3. NM_008064.3.
UniGeneiMm.4793.

Genome annotation databases

EnsembliENSMUST00000026666; ENSMUSP00000026666; ENSMUSG00000025579.
ENSMUST00000106259; ENSMUSP00000101866; ENSMUSG00000025579.
GeneIDi14387.
KEGGimmu:14387.
UCSCiuc007mqg.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U49351 mRNA. Translation: AAB06943.1 .
AK052211 mRNA. Translation: BAC34888.1 .
AK088481 mRNA. Translation: BAC40382.1 .
AK139333 mRNA. Translation: BAE23960.1 .
AK146538 mRNA. Translation: BAE27243.1 .
AK150970 mRNA. Translation: BAE30001.1 .
BC010210 mRNA. Translation: AAH10210.1 .
CCDSi CCDS25713.1.
RefSeqi NP_001152796.1. NM_001159324.1.
NP_032090.3. NM_008064.3.
UniGenei Mm.4793.

3D structure databases

ProteinModelPortali P70699.
SMRi P70699. Positions 89-953.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P70699. 1 interaction.
MINTi MINT-4100796.

Chemistry

BindingDBi P70699.
ChEMBLi CHEMBL1667668.

Protein family/group databases

CAZyi GH31. Glycoside Hydrolase Family 31.

PTM databases

PhosphoSitei P70699.

Proteomic databases

MaxQBi P70699.
PaxDbi P70699.
PRIDEi P70699.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000026666 ; ENSMUSP00000026666 ; ENSMUSG00000025579 .
ENSMUST00000106259 ; ENSMUSP00000101866 ; ENSMUSG00000025579 .
GeneIDi 14387.
KEGGi mmu:14387.
UCSCi uc007mqg.2. mouse.

Organism-specific databases

CTDi 2548.
MGIi MGI:95609. Gaa.

Phylogenomic databases

eggNOGi COG1501.
GeneTreei ENSGT00760000119229.
HOGENOMi HOG000041175.
HOVERGENi HBG006297.
InParanoidi P70699.
KOi K12316.
OMAi SSEMGYT.
OrthoDBi EOG77HDD0.
PhylomeDBi P70699.
TreeFami TF314577.

Miscellaneous databases

ChiTaRSi Gaa. mouse.
NextBioi 285901.
PROi P70699.
SOURCEi Search...

Gene expression databases

Bgeei P70699.
CleanExi MM_GAA.
ExpressionAtlasi P70699. baseline and differential.
Genevestigatori P70699.

Family and domain databases

Gene3Di 4.10.110.10. 1 hit.
InterProi IPR011013. Gal_mutarotase_SF_dom.
IPR000322. Glyco_hydro_31.
IPR025887. Glyco_hydro_31_N_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR000519. P_trefoil.
IPR017957. P_trefoil_CS.
[Graphical view ]
Pfami PF13802. Gal_mutarotas_2. 1 hit.
PF01055. Glyco_hydro_31. 1 hit.
PF00088. Trefoil. 1 hit.
[Graphical view ]
SMARTi SM00018. PD. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 2 hits.
SSF74650. SSF74650. 1 hit.
PROSITEi PS00129. GLYCOSYL_HYDROL_F31_1. 1 hit.
PS00707. GLYCOSYL_HYDROL_F31_2. 1 hit.
PS00025. P_TREFOIL_1. 1 hit.
PS51448. P_TREFOIL_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of the mouse liver cDNA encoding lysosomal alpha-glucosidase."
    Ding J.H., Yang B.Z., Reuser A.J.J., Roe C.R.
    Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
    Tissue: Liver.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Bone marrow, Brain cortex, Heart and Thymus.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.
  4. Lubec G., Sunyer B., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 376-385, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: OF1.
    Tissue: Hippocampus.
  5. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
    Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
    Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-140 AND ASN-470.

Entry informationi

Entry nameiLYAG_MOUSE
AccessioniPrimary (citable) accession number: P70699
Secondary accession number(s): Q3UJB2, Q8BGI6, Q91Z45
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: August 16, 2004
Last modified: November 26, 2014
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3