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P70699

- LYAG_MOUSE

UniProt

P70699 - LYAG_MOUSE

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Protein
Lysosomal alpha-glucosidase
Gene
Gaa
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Essential for the degradation of glygogen to glucose in lysosomes.

Catalytic activityi

Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei518 – 5181Nucleophile By similarity
Active sitei521 – 5211 By similarity

GO - Molecular functioni

  1. alpha-glucosidase activity Source: MGI
  2. carbohydrate binding Source: InterPro
  3. maltose alpha-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. cardiac muscle contraction Source: Ensembl
  2. diaphragm contraction Source: MGI
  3. glycogen catabolic process Source: MGI
  4. glycogen metabolic process Source: MGI
  5. heart morphogenesis Source: MGI
  6. locomotory behavior Source: MGI
  7. lysosome organization Source: MGI
  8. muscle cell cellular homeostasis Source: MGI
  9. neuromuscular process controlling balance Source: MGI
  10. neuromuscular process controlling posture Source: MGI
  11. regulation of the force of heart contraction Source: MGI
  12. striated muscle contraction Source: MGI
  13. tissue development Source: MGI
  14. tongue morphogenesis Source: Ensembl
  15. vacuolar sequestering Source: Ensembl
  16. ventricular cardiac muscle tissue morphogenesis Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH31. Glycoside Hydrolase Family 31.

Names & Taxonomyi

Protein namesi
Recommended name:
Lysosomal alpha-glucosidase (EC:3.2.1.20)
Alternative name(s):
Acid maltase
Gene namesi
Name:Gaa
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:95609. Gaa.

Subcellular locationi

GO - Cellular componenti

  1. lysosomal membrane Source: UniProtKB-SubCell
  2. lysosome Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727 Reviewed prediction
Add
BLAST
Propeptidei28 – 6942 By similarity
PRO_0000018569Add
BLAST
Chaini70 – 953884Lysosomal alpha-glucosidase
PRO_0000018570Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi82 ↔ 109 By similarity
Disulfide bondi92 ↔ 108 By similarity
Disulfide bondi103 ↔ 127 By similarity
Glycosylationi140 – 1401N-linked (GlcNAc...)1 Publication
Glycosylationi233 – 2331N-linked (GlcNAc...) Reviewed prediction
Glycosylationi390 – 3901N-linked (GlcNAc...) Reviewed prediction
Glycosylationi470 – 4701N-linked (GlcNAc...)1 Publication
Glycosylationi883 – 8831N-linked (GlcNAc...) Reviewed prediction
Glycosylationi926 – 9261N-linked (GlcNAc...) Reviewed prediction
Glycosylationi933 – 9331N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP70699.
PaxDbiP70699.
PRIDEiP70699.

PTM databases

PhosphoSiteiP70699.

Expressioni

Gene expression databases

ArrayExpressiP70699.
BgeeiP70699.
CleanExiMM_GAA.
GenevestigatoriP70699.

Interactioni

Protein-protein interaction databases

IntActiP70699. 1 interaction.
MINTiMINT-4100796.

Structurei

3D structure databases

ProteinModelPortaliP70699.
SMRiP70699. Positions 89-953.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini80 – 13152P-type
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1501.
GeneTreeiENSGT00740000115444.
HOGENOMiHOG000041175.
HOVERGENiHBG006297.
InParanoidiP70699.
KOiK12316.
OMAiSSEMGYT.
OrthoDBiEOG77HDD0.
PhylomeDBiP70699.
TreeFamiTF314577.

Family and domain databases

Gene3Di4.10.110.10. 1 hit.
InterProiIPR011013. Gal_mutarotase_SF_dom.
IPR000322. Glyco_hydro_31.
IPR025887. Glyco_hydro_31_N_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR000519. P_trefoil.
IPR017957. P_trefoil_CS.
[Graphical view]
PfamiPF13802. Gal_mutarotas_2. 1 hit.
PF01055. Glyco_hydro_31. 1 hit.
PF00088. Trefoil. 1 hit.
[Graphical view]
SMARTiSM00018. PD. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 2 hits.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00129. GLYCOSYL_HYDROL_F31_1. 1 hit.
PS00707. GLYCOSYL_HYDROL_F31_2. 1 hit.
PS00025. P_TREFOIL_1. 1 hit.
PS51448. P_TREFOIL_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P70699-1 [UniParc]FASTAAdd to Basket

« Hide

MNIRKPLCSN SVVGACTLIS LTTAVILGHL MLRELMLLPQ DLHESSSGLW    50
KTYRPHHQEG YKPGPLHIQE QTEQPKEAPT QCDVPPSSRF DCAPDKGISQ 100
EQCEARGCCY VPAGQVLKEP QIGQPWCFFP PSYPSYRLEN LSSTESGYTA 150
TLTRTSPTFF PKDVLTLQLE VLMETDSRLH FKIKDPASKR YEVPLETPRV 200
LSQAPSPLYS VEFSEEPFGV IVRRKLGGRV LLNTTVAPLF FADQFLQLST 250
SLPSQHITGL GEHLSPLMLS TDWARITLWN RDTPPSQGTN LYGSHPFYLA 300
LEDGGLAHGV FLLNSNAMDV ILQPSPALTW RSTGGILDVY VFLGPEPKSV 350
VQQYLDVVGY PFMPPYWGLG FHLCRWGYSS TAIVRQVVEN MTRTHFPLDV 400
QWNDLDYMDA RRDFTFNQDS FADFPDMVRE LHQDGRRYMM IVDPAISSAG 450
PAGSYRPYDE GLRRGVFITN ETGQPLIGKV WPGTTAFPDF TNPETLDWWQ 500
DMVSEFHAQV PFDGMWLDMN EPSNFVRGSQ QGCPNNELEN PPYVPGVVGG 550
ILQAATICAS SHQFLSTHYN LHNLYGLTEA IASSRALVKT RGTRPFVISR 600
STFSGHGRYA GHWTGDVRSS WEHLAYSVPD ILQFNLLGVP LVGADICGFI 650
GDTSEELCVR WTQLGAFYPF MRNHNDLNSV PQEPYRFSET AQQAMRKAFA 700
LRYALLPYLY TLFHRAHVRG DTVARPLFLE FPEDPSTWSV DRQLLWGPAL 750
LITPVLEPGK TEVTGYFPKG TWYNMQMVSV DSLGTLPSPS SASSFRSAVQ 800
SKGQWLTLEA PLDTINVHLR EGYIIPLQGP SLTTTESRKQ PMALAVALTA 850
SGEADGELFW DDGESLAVLE RGAYTLVTFS AKNNTIVNKL VRVTKEGAEL 900
QLREVTVLGV ATAPTQVLSN GIPVSNFTYS PDNKSLAIPV SLLMGELFQI 950
SWS 953
Length:953
Mass (Da):106,248
Last modified:August 16, 2004 - v2
Checksum:i956B89685FB5FF81
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti62 – 621K → E in AAB06943. 1 Publication
Sequence conflicti254 – 2541S → A in AAB06943. 1 Publication
Sequence conflicti430 – 4312EL → DV in AAB06943. 1 Publication
Sequence conflicti434 – 4341D → G1 Publication
Sequence conflicti434 – 4341D → G1 Publication
Sequence conflicti481 – 4811W → C in AAB06943. 1 Publication
Sequence conflicti615 – 6151G → E in AAB06943. 1 Publication
Sequence conflicti619 – 6191S → T in AAB06943. 1 Publication
Sequence conflicti732 – 7321P → R in AAB06943. 1 Publication
Sequence conflicti777 – 7771M → V1 Publication
Sequence conflicti777 – 7771M → V1 Publication
Sequence conflicti871 – 8711R → H in AAB06943. 1 Publication
Sequence conflicti903 – 9031R → K in AAB06943. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U49351 mRNA. Translation: AAB06943.1.
AK052211 mRNA. Translation: BAC34888.1.
AK088481 mRNA. Translation: BAC40382.1.
AK139333 mRNA. Translation: BAE23960.1.
AK146538 mRNA. Translation: BAE27243.1.
AK150970 mRNA. Translation: BAE30001.1.
BC010210 mRNA. Translation: AAH10210.1.
CCDSiCCDS25713.1.
RefSeqiNP_001152796.1. NM_001159324.1.
NP_032090.3. NM_008064.3.
UniGeneiMm.4793.

Genome annotation databases

EnsembliENSMUST00000026666; ENSMUSP00000026666; ENSMUSG00000025579.
ENSMUST00000106259; ENSMUSP00000101866; ENSMUSG00000025579.
GeneIDi14387.
KEGGimmu:14387.
UCSCiuc007mqg.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U49351 mRNA. Translation: AAB06943.1 .
AK052211 mRNA. Translation: BAC34888.1 .
AK088481 mRNA. Translation: BAC40382.1 .
AK139333 mRNA. Translation: BAE23960.1 .
AK146538 mRNA. Translation: BAE27243.1 .
AK150970 mRNA. Translation: BAE30001.1 .
BC010210 mRNA. Translation: AAH10210.1 .
CCDSi CCDS25713.1.
RefSeqi NP_001152796.1. NM_001159324.1.
NP_032090.3. NM_008064.3.
UniGenei Mm.4793.

3D structure databases

ProteinModelPortali P70699.
SMRi P70699. Positions 89-953.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P70699. 1 interaction.
MINTi MINT-4100796.

Chemistry

BindingDBi P70699.
ChEMBLi CHEMBL1667668.

Protein family/group databases

CAZyi GH31. Glycoside Hydrolase Family 31.

PTM databases

PhosphoSitei P70699.

Proteomic databases

MaxQBi P70699.
PaxDbi P70699.
PRIDEi P70699.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000026666 ; ENSMUSP00000026666 ; ENSMUSG00000025579 .
ENSMUST00000106259 ; ENSMUSP00000101866 ; ENSMUSG00000025579 .
GeneIDi 14387.
KEGGi mmu:14387.
UCSCi uc007mqg.2. mouse.

Organism-specific databases

CTDi 2548.
MGIi MGI:95609. Gaa.

Phylogenomic databases

eggNOGi COG1501.
GeneTreei ENSGT00740000115444.
HOGENOMi HOG000041175.
HOVERGENi HBG006297.
InParanoidi P70699.
KOi K12316.
OMAi SSEMGYT.
OrthoDBi EOG77HDD0.
PhylomeDBi P70699.
TreeFami TF314577.

Miscellaneous databases

NextBioi 285901.
PROi P70699.
SOURCEi Search...

Gene expression databases

ArrayExpressi P70699.
Bgeei P70699.
CleanExi MM_GAA.
Genevestigatori P70699.

Family and domain databases

Gene3Di 4.10.110.10. 1 hit.
InterProi IPR011013. Gal_mutarotase_SF_dom.
IPR000322. Glyco_hydro_31.
IPR025887. Glyco_hydro_31_N_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR000519. P_trefoil.
IPR017957. P_trefoil_CS.
[Graphical view ]
Pfami PF13802. Gal_mutarotas_2. 1 hit.
PF01055. Glyco_hydro_31. 1 hit.
PF00088. Trefoil. 1 hit.
[Graphical view ]
SMARTi SM00018. PD. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 2 hits.
SSF74650. SSF74650. 1 hit.
PROSITEi PS00129. GLYCOSYL_HYDROL_F31_1. 1 hit.
PS00707. GLYCOSYL_HYDROL_F31_2. 1 hit.
PS00025. P_TREFOIL_1. 1 hit.
PS51448. P_TREFOIL_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of the mouse liver cDNA encoding lysosomal alpha-glucosidase."
    Ding J.H., Yang B.Z., Reuser A.J.J., Roe C.R.
    Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
    Tissue: Liver.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Bone marrow, Brain cortex, Heart and Thymus.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.
  4. Lubec G., Sunyer B., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 376-385, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: OF1.
    Tissue: Hippocampus.
  5. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
    Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
    Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-140 AND ASN-470.

Entry informationi

Entry nameiLYAG_MOUSE
AccessioniPrimary (citable) accession number: P70699
Secondary accession number(s): Q3UJB2, Q8BGI6, Q91Z45
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: August 16, 2004
Last modified: July 9, 2014
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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