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Protein

CTP synthase 1

Gene

Ctps1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This enzyme is involved in the de novo synthesis of CTP, a precursor of DNA, RNA and phospholipids. Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as a source of nitrogen. This enzyme and its product, CTP, play a crucial role in the proliferation of activated lymphocytes and therefore in immunity.By similarity

Catalytic activityi

ATP + UTP + L-glutamine = ADP + phosphate + CTP + L-glutamate.By similarity

Enzyme regulationi

Activated by GTP and inhibited by CTP.

Pathwayi: CTP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes CTP from UDP.By similarity
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. CTP synthase 2 (Ctps2), CTP synthase 1 (Ctps1)
This subpathway is part of the pathway CTP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CTP from UDP, the pathway CTP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei399 – 3991For GATase activityBy similarity
Active sitei526 – 5261For GATase activityBy similarity
Active sitei528 – 5281For GATase activityBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Immunity, Pyrimidine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.3.4.2. 3474.
ReactomeiR-MMU-499943. Synthesis and interconversion of nucleotide di- and triphosphates.
UniPathwayiUPA00159; UER00277.

Names & Taxonomyi

Protein namesi
Recommended name:
CTP synthase 1Curated (EC:6.3.4.2By similarity)
Alternative name(s):
CTP synthetase 1
UTP--ammonia ligase 1
Gene namesi
Name:Ctps1
Synonyms:CtpsImported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1858304. Ctps.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 591591CTP synthase 1PRO_0000138276Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei100 – 1001N6-acetyllysineBy similarity
Modified residuei562 – 5621PhosphoserineBy similarity
Modified residuei568 – 5681PhosphoserineBy similarity
Modified residuei571 – 5711PhosphoserineBy similarity
Modified residuei573 – 5731PhosphoserineBy similarity
Modified residuei574 – 5741PhosphoserineCombined sources
Modified residuei575 – 5751PhosphoserineCombined sources
Modified residuei578 – 5781PhosphoserineCombined sources
Modified residuei587 – 5871PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP70698.
MaxQBiP70698.
PaxDbiP70698.
PeptideAtlasiP70698.
PRIDEiP70698.

PTM databases

iPTMnetiP70698.
PhosphoSiteiP70698.

Expressioni

Gene expression databases

BgeeiENSMUSG00000028633.
CleanExiMM_CTPS.
GenevisibleiP70698. MM.

Interactioni

Protein-protein interaction databases

BioGridi206180. 3 interactions.
IntActiP70698. 3 interactions.
MINTiMINT-4109278.
STRINGi10090.ENSMUSP00000030381.

Structurei

3D structure databases

ProteinModelPortaliP70698.
SMRiP70698. Positions 1-273, 296-562.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini300 – 554255Glutamine amidotransferase type-1Add
BLAST

Sequence similaritiesi

Belongs to the CTP synthase family.Curated

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiKOG2387. Eukaryota.
COG0504. LUCA.
GeneTreeiENSGT00390000012473.
HOGENOMiHOG000077514.
HOVERGENiHBG002243.
InParanoidiP70698.
KOiK01937.
OMAiSCSERHR.
OrthoDBiEOG091G03OK.
PhylomeDBiP70698.
TreeFamiTF300379.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
3.40.50.880. 1 hit.
HAMAPiMF_01227. PyrG. 1 hit.
InterProiIPR029062. Class_I_gatase-like.
IPR004468. CTP_synthase.
IPR017456. CTP_synthase_N.
IPR017926. GATASE.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11550. PTHR11550. 1 hit.
PfamiPF06418. CTP_synth_N. 1 hit.
PF00117. GATase. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00337. PyrG. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P70698-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKYILVTGGV ISGIGKGVIA SSVGTILKSC GLHVTSIKID PYINIDAGTF
60 70 80 90 100
SPYEHGEVFV LDDGGEVDLD LGNYERFLDI RLTKDNNLTT GKIYQYVINK
110 120 130 140 150
ERKGDYLGKT VQVVPHITDA IQEWVMRQAL IPVDEDGLEP QVCVIELGGT
160 170 180 190 200
VGDIESMPFI EAFRQFQFKV KRENFCNIHV SLVPQPSSTG EQKTKPTQNS
210 220 230 240 250
VRELRGLGLS PDLVVCRCSN PLDTSVKEKI SMFCHVEPEQ VICVHDVSSI
260 270 280 290 300
YRVPLLLEEQ GVVDYFLRRL DLPIERQSRK MLMKWKEMAD RYDRLLETCS
310 320 330 340 350
IALVGKYTKF SDSYASVIKA LEHSALAINH KLEIKYIDST DLEPSTLQEE
360 370 380 390 400
PVRYHEAWQK LCSAHGVLVP GGFGVRGTEG KIQAIAWARK QKKPFLGVCL
410 420 430 440 450
GMQLAVVEFS RNVLGWQDAN STEFDPKTSH PVVIDMPEHN PGQMGGTMRL
460 470 480 490 500
GKRRTLFQTK NSVMRKLYGD TDYLEERHRH RFEVNPVLKK CLEEQGLKFV
510 520 530 540 550
GQDVEGERME IVELEDHPFF VGVQYHPEFL SRPIKPSPPY FGLLLASVGR
560 570 580 590
LPHYLQKGCR LSPRDTYSDR SGSSSPDSEI TELKFPSISQ D
Length:591
Mass (Da):66,682
Last modified:July 25, 2006 - v2
Checksum:i81083429870BF0DE
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti37 – 371I → F in AAB06942 (Ref. 1) Curated
Sequence conflicti308 – 3081T → P in AAB06942 (Ref. 1) Curated
Sequence conflicti388 – 3881A → S in AAB06942 (Ref. 1) Curated
Sequence conflicti402 – 4021M → I in AAB06942 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U49350 mRNA. Translation: AAB06942.1.
AK148259 mRNA. Translation: BAE28443.1.
BC006698 mRNA. Translation: AAH06698.1.
CCDSiCCDS18591.1.
RefSeqiNP_058028.2. NM_016748.2.
XP_006503288.1. XM_006503225.2.
UniGeneiMm.1815.

Genome annotation databases

EnsembliENSMUST00000030381; ENSMUSP00000030381; ENSMUSG00000028633.
GeneIDi51797.
KEGGimmu:51797.
UCSCiuc008unj.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U49350 mRNA. Translation: AAB06942.1.
AK148259 mRNA. Translation: BAE28443.1.
BC006698 mRNA. Translation: AAH06698.1.
CCDSiCCDS18591.1.
RefSeqiNP_058028.2. NM_016748.2.
XP_006503288.1. XM_006503225.2.
UniGeneiMm.1815.

3D structure databases

ProteinModelPortaliP70698.
SMRiP70698. Positions 1-273, 296-562.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi206180. 3 interactions.
IntActiP70698. 3 interactions.
MINTiMINT-4109278.
STRINGi10090.ENSMUSP00000030381.

PTM databases

iPTMnetiP70698.
PhosphoSiteiP70698.

Proteomic databases

EPDiP70698.
MaxQBiP70698.
PaxDbiP70698.
PeptideAtlasiP70698.
PRIDEiP70698.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030381; ENSMUSP00000030381; ENSMUSG00000028633.
GeneIDi51797.
KEGGimmu:51797.
UCSCiuc008unj.2. mouse.

Organism-specific databases

CTDi51797.
MGIiMGI:1858304. Ctps.

Phylogenomic databases

eggNOGiKOG2387. Eukaryota.
COG0504. LUCA.
GeneTreeiENSGT00390000012473.
HOGENOMiHOG000077514.
HOVERGENiHBG002243.
InParanoidiP70698.
KOiK01937.
OMAiSCSERHR.
OrthoDBiEOG091G03OK.
PhylomeDBiP70698.
TreeFamiTF300379.

Enzyme and pathway databases

UniPathwayiUPA00159; UER00277.
BRENDAi6.3.4.2. 3474.
ReactomeiR-MMU-499943. Synthesis and interconversion of nucleotide di- and triphosphates.

Miscellaneous databases

PROiP70698.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028633.
CleanExiMM_CTPS.
GenevisibleiP70698. MM.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
3.40.50.880. 1 hit.
HAMAPiMF_01227. PyrG. 1 hit.
InterProiIPR029062. Class_I_gatase-like.
IPR004468. CTP_synthase.
IPR017456. CTP_synthase_N.
IPR017926. GATASE.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11550. PTHR11550. 1 hit.
PfamiPF06418. CTP_synth_N. 1 hit.
PF00117. GATase. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00337. PyrG. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPYRG1_MOUSE
AccessioniPrimary (citable) accession number: P70698
Secondary accession number(s): Q922Y4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 25, 2006
Last modified: September 7, 2016
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.