Reviewed, UniProtKB/Swiss-Prot P70695 (F16P2_MOUSE)

Names and origin

Protein names

Recommended name:
    Fructose-1,6-bisphosphatase isozyme 2
      Short name=FBPase 2
    EC=3.1.3.11
Alternative name(s):
    D-fructose-1,6-bisphosphate 1-phosphohydrolase 2
    RAE-30

Gene names

Name:

Fbp2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May be involved in early differentiation.
Catalytic activity	D-fructose 1,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate.
Cofactor	Binds 3 magnesium ions per subunit By similarity.
Enzyme regulation	Subject to complex allosteric regulation. The enzyme can assume an active R-state, or an inactive T-state. Intermediate conformations may exist. AMP acts as allosteric inhibitor. Fructose-2,6-biphosphate acts as competitive inhibitor By similarity.
Pathway	Carbohydrate biosynthesis; gluconeogenesis.
Subunit structure	Homotetramer By similarity.
Tissue specificity	Placenta and adult intestine.
Induction	By retinoic acid.
Sequence similarities	Belongs to the FBPase class 1 family.
Sequence caution	The sequence BAA07678.1 differs from that shown. Reason: Frameshift at position 321.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Gluconeogenesis
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Allosteric enzyme
Gene Ontology (GO)
Biological process	gluconeogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	fructose 1,6-bisphosphate 1-phosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 339	339	Fructose-1,6-bisphosphatase isozyme 2		PRO_0000200505
Regions
	Nucleotide binding	18 – 22	5	AMP By similarity
	Nucleotide binding	28 – 32	5	AMP By similarity
	Nucleotide binding	113 – 114	2	AMP By similarity
	Region	122 – 125	4	Substrate binding By similarity
	Region	213 – 216	4	Substrate binding By similarity
	Region	244 – 249	6	Substrate binding By similarity
	Region	275 – 277	3	Substrate binding By similarity
Sites
	Metal binding	69	1	Magnesium 1 By similarity
	Metal binding	98	1	Magnesium 1 By similarity
	Metal binding	98	1	Magnesium 2 By similarity
	Metal binding	119	1	Magnesium 2 By similarity
	Metal binding	119	1	Magnesium 3 By similarity
	Metal binding	121	1	Magnesium 2; via carbonyl oxygen By similarity
	Metal binding	122	1	Magnesium 3 By similarity
	Metal binding	281	1	Magnesium 3 By similarity
	Binding site	141	1	AMP By similarity
	Binding site	265	1	Substrate By similarity
Amino acid modifications
	Modified residue	216	1	Phosphotyrosine By similarity
Experimental info
	Sequence conflict	171	1	A → R in BAA07678. Ref.1
	Sequence conflict	239	1	E → A in AAH12720. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P70695-1 [UniParc]. Last modified September 27, 2005. Version 2. Checksum: 442A3C5D09017415	FASTA	339	36,947
	10 20 30 40 50 60 MTDRSPFETD MLTLTRYVME KGRQAKGTGE LTQLLNSMLT AIKAISSAVR KAGLANLYGI 70 80 90 100 110 120 SGSVNVTGDE VKKLDVLSNS LVINMLQSSY STCVLVSEEN KEAVITAQER RGKYVVCFDP 130 140 150 160 170 180 LDGSSNIDCL ASIGTIFAIY RKTTEDEPSE KDALQPGRNI VAAGYALYGS ATLVALSTGQ 190 200 210 220 230 240 GVDLFMLDPA LGEFVLVEKD VRIKKKGKIF SLNEGYAKYF DAATAEYVQK KKFPEDGSEP 250 260 270 280 290 300 YGARYVGSMV ADVHRTLVYG GIFMYPANQK SPNGKLRLLY ECNPVAYIIE QAGGMATTGT 310 320 330 QPVLDVKPES IHQRVPLILG SPEDVQEYLS CVQRNQAGR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"One of the retinoic acid-inducible cDNA clones in mouse embryonal carcinoma F9 cells encodes a novel isoenzyme of fructose 1,6-bisphosphatase." Nomura M., Takihara Y., Yasunaga T., Shimada K. FEBS Lett. 348:201-205(1994) [PubMed: 8034042] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c.
[2]	"Structure and chromosomal localization of the human and mouse muscle fructose-1,6-bisphosphatase genes." Tillmann H., Stein S., Liehr T., Eschrich K. Gene 247:241-253(2000) [PubMed: 10773464] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Tissue: Skeletal muscle.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Colon.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D42083 mRNA. Translation: BAA07678.1. Frameshift. AJ132692 mRNA. Translation: CAB65243.1. AJ243020 Genomic DNA. Translation: CAB90667.1. AJ243021 Genomic DNA. Translation: CAB90668.1. AJ243022 Genomic DNA. Translation: CAB90669.1. AJ243023 Genomic DNA. Translation: CAB90670.1. AJ243024 Genomic DNA. Translation: CAB90671.1. AJ243025 Genomic DNA. Translation: CAB90672.1. AJ243026 Genomic DNA. Translation: CAB90673.1. AJ243027 Genomic DNA. Translation: CAB90674.1. AJ243028 Genomic DNA. Translation: CAB90675.1. AJ245381 Genomic DNA. Translation: CAB65260.1. AJ245382 Genomic DNA. Translation: CAB65261.1. AJ245383 Genomic DNA. Translation: CAB65262.1. BC012720 mRNA. Translation: AAH12720.1.
IPI	IPI00111954.
PIR	S46245.
RefSeq	NP_032020.2.
UniGene	Mm.391871
3D structure databases
SMR	P70695. Positions 7-337.
ModBase	Search...
Protein-protein interaction databases
STRING	P70695.
PTM databases
PhosphoSite	P70695.
Proteomic databases
PRIDE	P70695.
Genome annotation databases
Ensembl	ENSMUST00000021907; ENSMUSP00000021907; ENSMUSG00000021456; Mus musculus. [Genome view]
GeneID	14120.
KEGG	mmu:14120.
UCSC	uc007qxf.1. mouse.
Organism-specific databases
CTD	14120.
MGI	MGI:95491. Fbp2.
Phylogenomic databases
eggNOG	roNOG08567.
HOVERGEN	P70695.
InParanoid	P70695.
OMA	ITAKEKR.
OrthoDB	EOG9Z3905.
PhylomeDB	P70695.
Enzyme and pathway databases
BRENDA	3.1.3.11. 244.
Gene expression databases
ArrayExpress	P70695.
Bgee	P70695.
CleanEx	MM_FBP2.
Genevestigator	P70695.
GermOnline	ENSMUSG00000021456. Mus musculus.
Family and domain databases
InterPro	IPR000146. Fructose_bisphosphatase. IPR020548. Fructose_bisphosphatase_AS. [Graphical view]
PANTHER	PTHR11556. In_FB_phphtase. 1 hit.
Pfam	PF00316. FBPase. 1 hit. [Graphical view]
PRINTS	PR00115. F16BPHPHTASE.
PROSITE	PS00124. FBPASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	285188.
SOURCE	Search...

Entry information

Entry name

F16P2_MOUSE

Accession

Primary (citable) accession number: P70695
Secondary accession number(s): Q91X26 , Q9JK01, Q9JK02, Q9JK03, Q9JK04, Q9JK05, Q9JK06, Q9JK07, Q9JK08, Q9JK09, Q9QXB4, Q9QXB5, Q9QXB6, Q9QXD7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	September 27, 2005
Last modified:	January 19, 2010
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)