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P70695 (F16P2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Fructose-1,6-bisphosphatase isozyme 2

Short name=FBPase 2
EC=3.1.3.11
Alternative name(s):
D-fructose-1,6-bisphosphate 1-phosphohydrolase 2
RAE-30
Gene names
Name:Fbp2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length339 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

May be involved in early differentiation. HAMAP-Rule MF_01855

Catalytic activity

D-fructose 1,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate. HAMAP-Rule MF_01855

Cofactor

Binds 3 magnesium ions per subunit By similarity. HAMAP-Rule MF_01855

Enzyme regulation

Subject to complex allosteric regulation. The enzyme can assume an active R-state, or an inactive T-state. Intermediate conformations may exist. AMP acts as allosteric inhibitor. Fructose 2,6-bisphosphate acts as competitive inhibitor By similarity. HAMAP-Rule MF_01855

Pathway

Carbohydrate biosynthesis; gluconeogenesis. HAMAP-Rule MF_01855

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_01855

Tissue specificity

Placenta and adult intestine.

Induction

By retinoic acid. HAMAP-Rule MF_01855

Sequence similarities

Belongs to the FBPase class 1 family.

Sequence caution

The sequence BAA07678.1 differs from that shown. Reason: Frameshift at position 321.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Gluconeogenesis
   LigandMagnesium
Metal-binding
   Molecular functionHydrolase
   Technical termAllosteric enzyme
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processgluconeogenesis

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionfructose 1,6-bisphosphate 1-phosphatase activity

Inferred from electronic annotation. Source: UniProtKB-EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 339339Fructose-1,6-bisphosphatase isozyme 2 HAMAP-Rule MF_01855
PRO_0000200505

Regions

Nucleotide binding18 – 225AMP By similarity
Nucleotide binding28 – 325AMP By similarity
Nucleotide binding113 – 1142AMP By similarity
Region122 – 1254Substrate binding By similarity
Region213 – 2164Substrate binding By similarity
Region244 – 2496Substrate binding By similarity
Region275 – 2773Substrate binding By similarity

Sites

Metal binding691Magnesium 1 By similarity
Metal binding981Magnesium 1 By similarity
Metal binding981Magnesium 2 By similarity
Metal binding1191Magnesium 2 By similarity
Metal binding1191Magnesium 3 By similarity
Metal binding1211Magnesium 2; via carbonyl oxygen By similarity
Metal binding1221Magnesium 3 By similarity
Metal binding2811Magnesium 3 By similarity
Binding site1411AMP By similarity
Binding site2651Substrate By similarity

Experimental info

Sequence conflict1711A → R in BAA07678. Ref.1
Sequence conflict2391E → A in AAH12720. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P70695 [UniParc].

Last modified September 27, 2005. Version 2.
Checksum: 442A3C5D09017415

FASTA33936,947
        10         20         30         40         50         60 
MTDRSPFETD MLTLTRYVME KGRQAKGTGE LTQLLNSMLT AIKAISSAVR KAGLANLYGI 

        70         80         90        100        110        120 
SGSVNVTGDE VKKLDVLSNS LVINMLQSSY STCVLVSEEN KEAVITAQER RGKYVVCFDP 

       130        140        150        160        170        180 
LDGSSNIDCL ASIGTIFAIY RKTTEDEPSE KDALQPGRNI VAAGYALYGS ATLVALSTGQ 

       190        200        210        220        230        240 
GVDLFMLDPA LGEFVLVEKD VRIKKKGKIF SLNEGYAKYF DAATAEYVQK KKFPEDGSEP 

       250        260        270        280        290        300 
YGARYVGSMV ADVHRTLVYG GIFMYPANQK SPNGKLRLLY ECNPVAYIIE QAGGMATTGT 

       310        320        330 
QPVLDVKPES IHQRVPLILG SPEDVQEYLS CVQRNQAGR 

« Hide

References

« Hide 'large scale' references
[1]"One of the retinoic acid-inducible cDNA clones in mouse embryonal carcinoma F9 cells encodes a novel isoenzyme of fructose 1,6-bisphosphatase."
Nomura M., Takihara Y., Yasunaga T., Shimada K.
FEBS Lett. 348:201-205(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
[2]"Structure and chromosomal localization of the human and mouse muscle fructose-1,6-bisphosphatase genes."
Tillmann H., Stein S., Liehr T., Eschrich K.
Gene 247:241-253(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
Tissue: Skeletal muscle.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Colon.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D42083 mRNA. Translation: BAA07678.1. Frameshift.
AJ132692 mRNA. Translation: CAB65243.1.
AJ243020 Genomic DNA. Translation: CAB90667.1.
AJ243021 Genomic DNA. Translation: CAB90668.1.
AJ243022 Genomic DNA. Translation: CAB90669.1.
AJ243023 Genomic DNA. Translation: CAB90670.1.
AJ243024 Genomic DNA. Translation: CAB90671.1.
AJ243025 Genomic DNA. Translation: CAB90672.1.
AJ243026 Genomic DNA. Translation: CAB90673.1.
AJ243027 Genomic DNA. Translation: CAB90674.1.
AJ243028 Genomic DNA. Translation: CAB90675.1.
AJ245381 Genomic DNA. Translation: CAB65260.1.
AJ245382 Genomic DNA. Translation: CAB65261.1.
AJ245383 Genomic DNA. Translation: CAB65262.1.
BC012720 mRNA. Translation: AAH12720.1.
PIRS46245.
RefSeqNP_032020.2. NM_007994.3.
UniGeneMm.391871.

3D structure databases

ProteinModelPortalP70695.
SMRP70695. Positions 9-337.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP70695. 1 interaction.
MINTMINT-4094831.

PTM databases

PhosphoSiteP70695.

Proteomic databases

PaxDbP70695.
PRIDEP70695.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000021907; ENSMUSP00000021907; ENSMUSG00000021456.
GeneID14120.
KEGGmmu:14120.
UCSCuc007qxf.1. mouse.

Organism-specific databases

CTD8789.
MGIMGI:95491. Fbp2.

Phylogenomic databases

eggNOGCOG0158.
HOVERGENHBG005627.
InParanoidP70695.
KOK03841.
OMAVWTLTRF.
OrthoDBEOG7GJ6D9.
PhylomeDBP70695.
TreeFamTF314824.

Enzyme and pathway databases

UniPathwayUPA00138.

Gene expression databases

ArrayExpressP70695.
BgeeP70695.
CleanExMM_FBP2.
GenevestigatorP70695.

Family and domain databases

HAMAPMF_01855. FBPase_class1.
InterProIPR000146. FBPase_class-1/SBPase.
IPR028343. FBPtase.
IPR020548. Fructose_bisphosphatase_AS.
[Graphical view]
PANTHERPTHR11556. PTHR11556. 1 hit.
PfamPF00316. FBPase. 1 hit.
[Graphical view]
PIRSFPIRSF500210. FBPtase. 1 hit.
PIRSF000904. FBPtase_SBPase. 1 hit.
PRINTSPR00115. F16BPHPHTASE.
PROSITEPS00124. FBPASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio285188.
PROP70695.
SOURCESearch...

Entry information

Entry nameF16P2_MOUSE
AccessionPrimary (citable) accession number: P70695
Secondary accession number(s): Q91X26 expand/collapse secondary AC list , Q9JK01, Q9JK02, Q9JK03, Q9JK04, Q9JK05, Q9JK06, Q9JK07, Q9JK08, Q9JK09, Q9QXB4, Q9QXB5, Q9QXB6, Q9QXD7
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: September 27, 2005
Last modified: April 16, 2014
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot