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Protein

Estradiol 17 beta-dehydrogenase 5

Gene

Akr1c6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Active toward androgens, estrogens, and xenobiotic substrates. Also exhibits low 20 alpha-HSD activity. Shows a-stereospecificity in hydrogen transfer between cofactors and substrates (A-specific). Preferentially catalyzes the reduction of 4-androstenedione, 5-alpha-androstane-3,17-dione, androsterone and dehydroepiandrosterone to testosterone, dihydrotestosterone, 5-alpha-androstane-3-alpha,17-beta-diol and 5-androstene-3-beta,17-beta-diol, respectively.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei50NADPBy similarity1
Active sitei55Proton donorBy similarity1
Sitei84Lowers pKa of active site TyrBy similarity1
Binding sitei117SubstrateBy similarity1
Binding sitei190NADPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi20 – 24NADPBy similarity5
Nucleotide bindingi166 – 167NADPBy similarity2
Nucleotide bindingi216 – 221NADPBy similarity6
Nucleotide bindingi270 – 280NADPBy similarityAdd BLAST11

GO - Molecular functioni

GO - Biological processi

  • cellular response to jasmonic acid stimulus Source: MGI
  • daunorubicin metabolic process Source: MGI
  • doxorubicin metabolic process Source: MGI
  • negative regulation of mRNA splicing, via spliceosome Source: MGI
  • steroid biosynthetic process Source: MGI
  • steroid metabolic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

ReactomeiR-MMU-193368. Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.
R-MMU-193775. Synthesis of bile acids and bile salts via 24-hydroxycholesterol.
R-MMU-193807. Synthesis of bile acids and bile salts via 27-hydroxycholesterol.
R-MMU-2162123. Synthesis of Prostaglandins (PG) and Thromboxanes (TX).
R-MMU-5365859. RA biosynthesis pathway.
R-MMU-975634. Retinoid metabolism and transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Estradiol 17 beta-dehydrogenase 5 (EC:1.1.1.-)
Alternative name(s):
17-beta-HSD 5
Gene namesi
Name:Akr1c6
Synonyms:Hsd17b5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:1933427. Akr1c6.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001246521 – 323Estradiol 17 beta-dehydrogenase 5Add BLAST323

Post-translational modificationi

Three forms are detected, probably due to post-translational modifications.

Proteomic databases

PaxDbiP70694.
PeptideAtlasiP70694.
PRIDEiP70694.

2D gel databases

SWISS-2DPAGEP70694.

PTM databases

iPTMnetiP70694.
PhosphoSitePlusiP70694.

Expressioni

Tissue specificityi

Mainly found in liver. Also expressed weakly in kidney.1 Publication

Gene expression databases

BgeeiENSMUSG00000021210.
CleanExiMM_AKR1C6.
ExpressionAtlasiP70694. baseline and differential.
GenevisibleiP70694. MM.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

IntActiP70694. 3 interactors.
MINTiMINT-1866293.
STRINGi10090.ENSMUSP00000021630.

Structurei

3D structure databases

ProteinModelPortaliP70694.
SMRiP70694.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aldo/keto reductase family.Curated

Phylogenomic databases

eggNOGiKOG1577. Eukaryota.
COG0656. LUCA.
GeneTreeiENSGT00760000119041.
HOGENOMiHOG000250272.
HOVERGENiHBG000020.
InParanoidiP70694.
OMAiEWVNPNL.
OrthoDBiEOG091G0D69.
PhylomeDBiP70694.
TreeFamiTF106492.

Family and domain databases

CDDicd06660. Aldo_ket_red. 1 hit.
Gene3Di3.20.20.100. 1 hit.
InterProiIPR001395. Aldo/ket_red/Kv-b.
IPR018170. Aldo/ket_reductase_CS.
IPR020471. Aldo/keto_reductase.
IPR023210. NADP_OxRdtase_dom.
[Graphical view]
PANTHERiPTHR11732. PTHR11732. 2 hits.
PfamiPF00248. Aldo_ket_red. 1 hit.
[Graphical view]
PIRSFiPIRSF000097. AKR. 1 hit.
PRINTSiPR00069. ALDKETRDTASE.
SUPFAMiSSF51430. SSF51430. 1 hit.
PROSITEiPS00798. ALDOKETO_REDUCTASE_1. 1 hit.
PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P70694-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDSKQQTVRL SDGHFIPILG FGTYAPQEVP KSKATEATKI AIDAGFRHID
60 70 80 90 100
SASMYQNEKE VGLAIRSKIA DGTVKREDIF YTSKVWCTFH RPELVRVCLE
110 120 130 140 150
QSLKQLQLDY VDLYLIHFPM AMKPGENYLP KDENGKLIYD AVDICDTWEA
160 170 180 190 200
MEKCKDAGLA KSIGVSNFNR RQLEKILKKP GLKYKPVCNQ VECHPYLNQG
210 220 230 240 250
KLLDFCRSKD IVLVAYSALG SHREKQWVDQ SSPVLLDNPV LGSMAKKYNR
260 270 280 290 300
TPALIALRYQ LQRGVVVLAK SFSEKRIKEN MQVFEFQLTS EDMKVLDDLN
310 320
KNIRYISGSS FKDHPDFPFW DEY
Length:323
Mass (Da):37,048
Last modified:February 1, 1997 - v1
Checksum:i7A1B195E3074D36D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D45850 mRNA. Translation: BAA08285.1.
AF110414
, AF110408, AF110409, AF110410, AF110411, AF110412, AF110413 Genomic DNA. Translation: AAD41250.1.
BC056643 mRNA. Translation: AAH56643.1.
CCDSiCCDS26223.1.
PIRiA56424.
RefSeqiNP_085114.1. NM_030611.3.
UniGeneiMm.196666.

Genome annotation databases

EnsembliENSMUST00000021630; ENSMUSP00000021630; ENSMUSG00000021210.
GeneIDi83702.
KEGGimmu:83702.
UCSCiuc007pjo.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D45850 mRNA. Translation: BAA08285.1.
AF110414
, AF110408, AF110409, AF110410, AF110411, AF110412, AF110413 Genomic DNA. Translation: AAD41250.1.
BC056643 mRNA. Translation: AAH56643.1.
CCDSiCCDS26223.1.
PIRiA56424.
RefSeqiNP_085114.1. NM_030611.3.
UniGeneiMm.196666.

3D structure databases

ProteinModelPortaliP70694.
SMRiP70694.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP70694. 3 interactors.
MINTiMINT-1866293.
STRINGi10090.ENSMUSP00000021630.

PTM databases

iPTMnetiP70694.
PhosphoSitePlusiP70694.

2D gel databases

SWISS-2DPAGEP70694.

Proteomic databases

PaxDbiP70694.
PeptideAtlasiP70694.
PRIDEiP70694.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021630; ENSMUSP00000021630; ENSMUSG00000021210.
GeneIDi83702.
KEGGimmu:83702.
UCSCiuc007pjo.1. mouse.

Organism-specific databases

CTDi83702.
MGIiMGI:1933427. Akr1c6.

Phylogenomic databases

eggNOGiKOG1577. Eukaryota.
COG0656. LUCA.
GeneTreeiENSGT00760000119041.
HOGENOMiHOG000250272.
HOVERGENiHBG000020.
InParanoidiP70694.
OMAiEWVNPNL.
OrthoDBiEOG091G0D69.
PhylomeDBiP70694.
TreeFamiTF106492.

Enzyme and pathway databases

ReactomeiR-MMU-193368. Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.
R-MMU-193775. Synthesis of bile acids and bile salts via 24-hydroxycholesterol.
R-MMU-193807. Synthesis of bile acids and bile salts via 27-hydroxycholesterol.
R-MMU-2162123. Synthesis of Prostaglandins (PG) and Thromboxanes (TX).
R-MMU-5365859. RA biosynthesis pathway.
R-MMU-975634. Retinoid metabolism and transport.

Miscellaneous databases

PROiP70694.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021210.
CleanExiMM_AKR1C6.
ExpressionAtlasiP70694. baseline and differential.
GenevisibleiP70694. MM.

Family and domain databases

CDDicd06660. Aldo_ket_red. 1 hit.
Gene3Di3.20.20.100. 1 hit.
InterProiIPR001395. Aldo/ket_red/Kv-b.
IPR018170. Aldo/ket_reductase_CS.
IPR020471. Aldo/keto_reductase.
IPR023210. NADP_OxRdtase_dom.
[Graphical view]
PANTHERiPTHR11732. PTHR11732. 2 hits.
PfamiPF00248. Aldo_ket_red. 1 hit.
[Graphical view]
PIRSFiPIRSF000097. AKR. 1 hit.
PRINTSiPR00069. ALDKETRDTASE.
SUPFAMiSSF51430. SSF51430. 1 hit.
PROSITEiPS00798. ALDOKETO_REDUCTASE_1. 1 hit.
PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDHB5_MOUSE
AccessioniPrimary (citable) accession number: P70694
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: February 1, 1997
Last modified: November 2, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.