Reviewed, UniProtKB/Swiss-Prot P70694 (DHB5_MOUSE)

Names and origin

Protein names

Recommended name:
    Estradiol 17 beta-dehydrogenase 5
    EC=1.1.1.-
Alternative name(s):
    17-beta-HSD 5

Gene names

Name:	Akr1c6
Synonyms:	Hsd17b5

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	323 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Active toward androgens, estrogens, and xenobiotic substrates. Also exhibits low 20 alpha-HSD activity. Shows a-stereospecificity in hydrogen transfer between cofactors and substrates (A-specific). Preferentially catalyzes the reduction of 4-androstenedione, 5-alpha-androstane-3,17-dione, androsterone and dehydroepiandrosterone to testosterone, dihydrotestosterone, 5-alpha-androstane-3-alpha,17-beta-diol and 5-androstene-3-beta,17-beta-diol, respectively. Ref.2
Subunit structure	Monomer.
Tissue specificity	Mainly found in liver. Also expressed weakly in kidney. Ref.2
Post-translational modification	Three forms are detected, probably due to post-translational modifications.
Sequence similarities	Belongs to the aldo/keto reductase family.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW steroid biosynthetic process Inferred from direct assay. Source: MGI
Molecular function	aldo-keto reductase activity Inferred from direct assay. Source: MGI estradiol 17-beta-dehydrogenase activity Ref.1 Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 323	323	Estradiol 17 beta-dehydrogenase 5		PRO_0000124652
Regions
	Nucleotide binding	13 – 22	10	NADP Potential
	Nucleotide binding	217 – 280	64	NADP By similarity
Sites
	Active site	55	1	Proton donor By similarity
	Binding site	117	1	Substrate By similarity
	Site	84	1	Lowers pKa of active site Tyr By similarity
Amino acid modifications
	Modified residue	310	1	Phosphoserine Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P70694-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 7A1B195E3074D36D	FASTA	323	37,048
	10 20 30 40 50 60 MDSKQQTVRL SDGHFIPILG FGTYAPQEVP KSKATEATKI AIDAGFRHID SASMYQNEKE 70 80 90 100 110 120 VGLAIRSKIA DGTVKREDIF YTSKVWCTFH RPELVRVCLE QSLKQLQLDY VDLYLIHFPM 130 140 150 160 170 180 AMKPGENYLP KDENGKLIYD AVDICDTWEA MEKCKDAGLA KSIGVSNFNR RQLEKILKKP 190 200 210 220 230 240 GLKYKPVCNQ VECHPYLNQG KLLDFCRSKD IVLVAYSALG SHREKQWVDQ SSPVLLDNPV 250 260 270 280 290 300 LGSMAKKYNR TPALIALRYQ LQRGVVVLAK SFSEKRIKEN MQVFEFQLTS EDMKVLDDLN 310 320 KNIRYISGSS FKDHPDFPFW DEY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of mouse estradiol 17 beta-dehydrogenase (A-specific), a member of the aldoketoreductase family." Deyashiki Y., Ohshima K., Nakanishi M., Sato K., Matsuura K., Hara A. J. Biol. Chem. 270:10461-10467(1995) [PubMed: 7737980] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF N-TERMINUS, PARTIAL PROTEIN SEQUENCE. Strain: BALB/c. Tissue: Liver.
[2]	"Structure and activity of the murine type 5 17beta-hydroxysteroid dehydrogenase gene." Rheault P., Charbonneau A., Luu-The V. Biochim. Biophys. Acta 1447:17-24(1999) [PubMed: 10500239] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY. Strain: BALB/c. Tissue: Leukocyte.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Liver.
[4]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed: 17242355] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-310, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D45850 mRNA. Translation: BAA08285.1. AF110414 , AF110408, AF110409, AF110410, AF110411, AF110412, AF110413 Genomic DNA. Translation: AAD41250.1. BC056643 mRNA. Translation: AAH56643.1.
IPI	IPI00111950.
PIR	A56424.
RefSeq	NP_085114.1.
UniGene	Mm.196666
3D structure databases
SMR	P70694. Positions 2-323.
ModBase	Search...
Protein-protein interaction databases
STRING	P70694.
PTM databases
PhosphoSite	P70694.
2-D gel databases
SWISS-2DPAGE	P70694.
Proteomic databases
PRIDE	P70694.
Genome annotation databases
Ensembl	ENSMUST00000021630; ENSMUSP00000021630; ENSMUSG00000021210; Mus musculus. [Genome view]
GeneID	83702.
KEGG	mmu:83702.
UCSC	uc007pjo.1. mouse.
Organism-specific databases
CTD	83702.
MGI	MGI:1933427. Akr1c6.
Phylogenomic databases
eggNOG	roNOG15132.
HOGENOM	HBG605727.
HOVERGEN	P70694.
InParanoid	P70694.
OMA	IDSSHAY.
OrthoDB	EOG9B2WGJ.
PhylomeDB	P70694.
Gene expression databases
ArrayExpress	P70694.
Bgee	P70694.
CleanEx	MM_AKR1C6.
Genevestigator	P70694.
GermOnline	ENSMUSG00000021210. Mus musculus.
Family and domain databases
InterPro	IPR001395. Aldo/ket_red. IPR018170. Aldo/ket_reductase_CS. IPR020471. Aldo/keto_reductase_sg. [Graphical view]
Gene3D	G3DSA:3.20.20.100. Aldo/ket_red. 1 hit.
PANTHER	PTHR11732. Aldo/ket_red. 1 hit.
Pfam	PF00248. Aldo_ket_red. 1 hit. [Graphical view]
PRINTS	PR00069. ALDKETRDTASE.
PROSITE	PS00798. ALDOKETO_REDUCTASE_1. 1 hit. PS00062. ALDOKETO_REDUCTASE_2. 1 hit. PS00063. ALDOKETO_REDUCTASE_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	350738.
SOURCE	Search...

Entry information

Entry name

DHB5_MOUSE

Accession

Primary (citable) accession number: P70694

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	February 1, 1997
Last modified:	January 19, 2010
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)