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P70694 (DHB5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Estradiol 17 beta-dehydrogenase 5

EC=1.1.1.-
Alternative name(s):
17-beta-HSD 5
Gene names
Name:Akr1c6
Synonyms:Hsd17b5
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length323 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Active toward androgens, estrogens, and xenobiotic substrates. Also exhibits low 20 alpha-HSD activity. Shows a-stereospecificity in hydrogen transfer between cofactors and substrates (A-specific). Preferentially catalyzes the reduction of 4-androstenedione, 5-alpha-androstane-3,17-dione, androsterone and dehydroepiandrosterone to testosterone, dihydrotestosterone, 5-alpha-androstane-3-alpha,17-beta-diol and 5-androstene-3-beta,17-beta-diol, respectively. Ref.2

Subunit structure

Monomer.

Tissue specificity

Mainly found in liver. Also expressed weakly in kidney. Ref.2

Post-translational modification

Three forms are detected, probably due to post-translational modifications.

Sequence similarities

Belongs to the aldo/keto reductase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 323323Estradiol 17 beta-dehydrogenase 5
PRO_0000124652

Regions

Nucleotide binding20 – 245NADP By similarity
Nucleotide binding166 – 1672NADP By similarity
Nucleotide binding216 – 2216NADP By similarity
Nucleotide binding270 – 28011NADP By similarity

Sites

Active site551Proton donor By similarity
Binding site501NADP By similarity
Binding site1171Substrate By similarity
Binding site1901NADP By similarity
Site841Lowers pKa of active site Tyr By similarity

Sequences

Sequence LengthMass (Da)Tools
P70694 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 7A1B195E3074D36D

FASTA32337,048
        10         20         30         40         50         60 
MDSKQQTVRL SDGHFIPILG FGTYAPQEVP KSKATEATKI AIDAGFRHID SASMYQNEKE 

        70         80         90        100        110        120 
VGLAIRSKIA DGTVKREDIF YTSKVWCTFH RPELVRVCLE QSLKQLQLDY VDLYLIHFPM 

       130        140        150        160        170        180 
AMKPGENYLP KDENGKLIYD AVDICDTWEA MEKCKDAGLA KSIGVSNFNR RQLEKILKKP 

       190        200        210        220        230        240 
GLKYKPVCNQ VECHPYLNQG KLLDFCRSKD IVLVAYSALG SHREKQWVDQ SSPVLLDNPV 

       250        260        270        280        290        300 
LGSMAKKYNR TPALIALRYQ LQRGVVVLAK SFSEKRIKEN MQVFEFQLTS EDMKVLDDLN 

       310        320 
KNIRYISGSS FKDHPDFPFW DEY 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning and characterization of mouse estradiol 17 beta-dehydrogenase (A-specific), a member of the aldoketoreductase family."
Deyashiki Y., Ohshima K., Nakanishi M., Sato K., Matsuura K., Hara A.
J. Biol. Chem. 270:10461-10467(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF N-TERMINUS, PARTIAL PROTEIN SEQUENCE.
Strain: BALB/c.
Tissue: Liver.
[2]"Structure and activity of the murine type 5 17beta-hydroxysteroid dehydrogenase gene."
Rheault P., Charbonneau A., Luu-The V.
Biochim. Biophys. Acta 1447:17-24(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY.
Strain: BALB/c.
Tissue: Leukocyte.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D45850 mRNA. Translation: BAA08285.1.
AF110414 expand/collapse EMBL AC list , AF110408, AF110409, AF110410, AF110411, AF110412, AF110413 Genomic DNA. Translation: AAD41250.1.
BC056643 mRNA. Translation: AAH56643.1.
PIRA56424.
RefSeqNP_085114.1. NM_030611.3.
UniGeneMm.196666.

3D structure databases

ProteinModelPortalP70694.
SMRP70694. Positions 2-323.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP70694. 3 interactions.
MINTMINT-1866293.
STRING10090.ENSMUSP00000021630.

PTM databases

PhosphoSiteP70694.

2D gel databases

SWISS-2DPAGEP70694.

Proteomic databases

PaxDbP70694.
PRIDEP70694.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000021630; ENSMUSP00000021630; ENSMUSG00000021210.
GeneID83702.
KEGGmmu:83702.
UCSCuc007pjo.1. mouse.

Organism-specific databases

CTD83702.
MGIMGI:1933427. Akr1c6.

Phylogenomic databases

eggNOGCOG0656.
HOGENOMHOG000250272.
HOVERGENHBG000020.
InParanoidP70694.
OMARIASNID.
OrthoDBEOG70KGQF.
PhylomeDBP70694.
TreeFamTF106492.

Gene expression databases

BgeeP70694.
CleanExMM_AKR1C6.
GenevestigatorP70694.

Family and domain databases

Gene3D3.20.20.100. 1 hit.
InterProIPR001395. Aldo/ket_red.
IPR018170. Aldo/ket_reductase_CS.
IPR020471. Aldo/keto_reductase_subgr.
IPR023210. NADP_OxRdtase_dom.
[Graphical view]
PANTHERPTHR11732. PTHR11732. 1 hit.
PfamPF00248. Aldo_ket_red. 1 hit.
[Graphical view]
PIRSFPIRSF000097. AKR. 1 hit.
PRINTSPR00069. ALDKETRDTASE.
SUPFAMSSF51430. SSF51430. 1 hit.
PROSITEPS00798. ALDOKETO_REDUCTASE_1. 1 hit.
PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio350738.
PROP70694.
SOURCESearch...

Entry information

Entry nameDHB5_MOUSE
AccessionPrimary (citable) accession number: P70694
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: February 1, 1997
Last modified: April 16, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot