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P70694

- DHB5_MOUSE

UniProt

P70694 - DHB5_MOUSE

Protein

Estradiol 17 beta-dehydrogenase 5

Gene

Akr1c6

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
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    Functioni

    Active toward androgens, estrogens, and xenobiotic substrates. Also exhibits low 20 alpha-HSD activity. Shows a-stereospecificity in hydrogen transfer between cofactors and substrates (A-specific). Preferentially catalyzes the reduction of 4-androstenedione, 5-alpha-androstane-3,17-dione, androsterone and dehydroepiandrosterone to testosterone, dihydrotestosterone, 5-alpha-androstane-3-alpha,17-beta-diol and 5-androstene-3-beta,17-beta-diol, respectively.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei50 – 501NADPBy similarity
    Active sitei55 – 551Proton donorBy similarity
    Sitei84 – 841Lowers pKa of active site TyrBy similarity
    Binding sitei117 – 1171SubstrateBy similarity
    Binding sitei190 – 1901NADPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi20 – 245NADPBy similarity
    Nucleotide bindingi166 – 1672NADPBy similarity
    Nucleotide bindingi216 – 2216NADPBy similarity
    Nucleotide bindingi270 – 28011NADPBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. aldo-keto reductase (NADP) activity Source: MGI
    2. estradiol 17-beta-dehydrogenase activity Source: MGI

    GO - Biological processi

    1. steroid biosynthetic process Source: MGI
    2. steroid metabolic process Source: MGI

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis

    Keywords - Ligandi

    NADP

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Estradiol 17 beta-dehydrogenase 5 (EC:1.1.1.-)
    Alternative name(s):
    17-beta-HSD 5
    Gene namesi
    Name:Akr1c6
    Synonyms:Hsd17b5
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 13

    Organism-specific databases

    MGIiMGI:1933427. Akr1c6.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 323323Estradiol 17 beta-dehydrogenase 5PRO_0000124652Add
    BLAST

    Post-translational modificationi

    Three forms are detected, probably due to post-translational modifications.

    Proteomic databases

    MaxQBiP70694.
    PaxDbiP70694.
    PRIDEiP70694.

    2D gel databases

    SWISS-2DPAGEP70694.

    PTM databases

    PhosphoSiteiP70694.

    Expressioni

    Tissue specificityi

    Mainly found in liver. Also expressed weakly in kidney.1 Publication

    Gene expression databases

    BgeeiP70694.
    CleanExiMM_AKR1C6.
    GenevestigatoriP70694.

    Interactioni

    Subunit structurei

    Monomer.

    Protein-protein interaction databases

    IntActiP70694. 3 interactions.
    MINTiMINT-1866293.
    STRINGi10090.ENSMUSP00000021630.

    Structurei

    3D structure databases

    ProteinModelPortaliP70694.
    SMRiP70694. Positions 2-323.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the aldo/keto reductase family.Curated

    Phylogenomic databases

    eggNOGiCOG0656.
    HOGENOMiHOG000250272.
    HOVERGENiHBG000020.
    InParanoidiP70694.
    OMAiEMREDED.
    OrthoDBiEOG70KGQF.
    PhylomeDBiP70694.
    TreeFamiTF106492.

    Family and domain databases

    Gene3Di3.20.20.100. 1 hit.
    InterProiIPR001395. Aldo/ket_red.
    IPR018170. Aldo/ket_reductase_CS.
    IPR020471. Aldo/keto_reductase_subgr.
    IPR023210. NADP_OxRdtase_dom.
    [Graphical view]
    PANTHERiPTHR11732. PTHR11732. 1 hit.
    PfamiPF00248. Aldo_ket_red. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000097. AKR. 1 hit.
    PRINTSiPR00069. ALDKETRDTASE.
    SUPFAMiSSF51430. SSF51430. 1 hit.
    PROSITEiPS00798. ALDOKETO_REDUCTASE_1. 1 hit.
    PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
    PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P70694-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDSKQQTVRL SDGHFIPILG FGTYAPQEVP KSKATEATKI AIDAGFRHID    50
    SASMYQNEKE VGLAIRSKIA DGTVKREDIF YTSKVWCTFH RPELVRVCLE 100
    QSLKQLQLDY VDLYLIHFPM AMKPGENYLP KDENGKLIYD AVDICDTWEA 150
    MEKCKDAGLA KSIGVSNFNR RQLEKILKKP GLKYKPVCNQ VECHPYLNQG 200
    KLLDFCRSKD IVLVAYSALG SHREKQWVDQ SSPVLLDNPV LGSMAKKYNR 250
    TPALIALRYQ LQRGVVVLAK SFSEKRIKEN MQVFEFQLTS EDMKVLDDLN 300
    KNIRYISGSS FKDHPDFPFW DEY 323
    Length:323
    Mass (Da):37,048
    Last modified:February 1, 1997 - v1
    Checksum:i7A1B195E3074D36D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D45850 mRNA. Translation: BAA08285.1.
    AF110414
    , AF110408, AF110409, AF110410, AF110411, AF110412, AF110413 Genomic DNA. Translation: AAD41250.1.
    BC056643 mRNA. Translation: AAH56643.1.
    CCDSiCCDS26223.1.
    PIRiA56424.
    RefSeqiNP_085114.1. NM_030611.3.
    UniGeneiMm.196666.

    Genome annotation databases

    EnsembliENSMUST00000021630; ENSMUSP00000021630; ENSMUSG00000021210.
    GeneIDi83702.
    KEGGimmu:83702.
    UCSCiuc007pjo.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D45850 mRNA. Translation: BAA08285.1 .
    AF110414
    , AF110408 , AF110409 , AF110410 , AF110411 , AF110412 , AF110413 Genomic DNA. Translation: AAD41250.1 .
    BC056643 mRNA. Translation: AAH56643.1 .
    CCDSi CCDS26223.1.
    PIRi A56424.
    RefSeqi NP_085114.1. NM_030611.3.
    UniGenei Mm.196666.

    3D structure databases

    ProteinModelPortali P70694.
    SMRi P70694. Positions 2-323.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P70694. 3 interactions.
    MINTi MINT-1866293.
    STRINGi 10090.ENSMUSP00000021630.

    PTM databases

    PhosphoSitei P70694.

    2D gel databases

    SWISS-2DPAGE P70694.

    Proteomic databases

    MaxQBi P70694.
    PaxDbi P70694.
    PRIDEi P70694.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000021630 ; ENSMUSP00000021630 ; ENSMUSG00000021210 .
    GeneIDi 83702.
    KEGGi mmu:83702.
    UCSCi uc007pjo.1. mouse.

    Organism-specific databases

    CTDi 83702.
    MGIi MGI:1933427. Akr1c6.

    Phylogenomic databases

    eggNOGi COG0656.
    HOGENOMi HOG000250272.
    HOVERGENi HBG000020.
    InParanoidi P70694.
    OMAi EMREDED.
    OrthoDBi EOG70KGQF.
    PhylomeDBi P70694.
    TreeFami TF106492.

    Miscellaneous databases

    NextBioi 350738.
    PROi P70694.
    SOURCEi Search...

    Gene expression databases

    Bgeei P70694.
    CleanExi MM_AKR1C6.
    Genevestigatori P70694.

    Family and domain databases

    Gene3Di 3.20.20.100. 1 hit.
    InterProi IPR001395. Aldo/ket_red.
    IPR018170. Aldo/ket_reductase_CS.
    IPR020471. Aldo/keto_reductase_subgr.
    IPR023210. NADP_OxRdtase_dom.
    [Graphical view ]
    PANTHERi PTHR11732. PTHR11732. 1 hit.
    Pfami PF00248. Aldo_ket_red. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000097. AKR. 1 hit.
    PRINTSi PR00069. ALDKETRDTASE.
    SUPFAMi SSF51430. SSF51430. 1 hit.
    PROSITEi PS00798. ALDOKETO_REDUCTASE_1. 1 hit.
    PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
    PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and characterization of mouse estradiol 17 beta-dehydrogenase (A-specific), a member of the aldoketoreductase family."
      Deyashiki Y., Ohshima K., Nakanishi M., Sato K., Matsuura K., Hara A.
      J. Biol. Chem. 270:10461-10467(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF N-TERMINUS, PARTIAL PROTEIN SEQUENCE.
      Strain: BALB/c.
      Tissue: Liver.
    2. "Structure and activity of the murine type 5 17beta-hydroxysteroid dehydrogenase gene."
      Rheault P., Charbonneau A., Luu-The V.
      Biochim. Biophys. Acta 1447:17-24(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY.
      Strain: BALB/c.
      Tissue: Leukocyte.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Liver.

    Entry informationi

    Entry nameiDHB5_MOUSE
    AccessioniPrimary (citable) accession number: P70694
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1999
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 113 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3