ID   CXB6_MOUSE              Reviewed;         261 AA.
AC   P70689;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   24-JAN-2024, entry version 164.
DE   RecName: Full=Gap junction beta-6 protein;
DE   AltName: Full=Connexin-30;
DE            Short=Cx30;
GN   Name=Gjb6; Synonyms=Cxn-30;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=C57BL/6J;
RX   PubMed=8663509; DOI=10.1074/jbc.271.30.17903;
RA   Dahl E., Manthey D., Chen Y., Schwarz H.-J., Chang Y.S., Lalley P.A.,
RA   Nicholson B.J., Willecke K.;
RT   "Molecular cloning and functional expression of mouse connexin-30, a gap
RT   junction gene highly expressed in adult brain and skin.";
RL   J. Biol. Chem. 271:17903-17910(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J; TISSUE=Bone, and Mammary gland;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [3]
RP   INTERACTION WITH CNST.
RX   PubMed=19864490; DOI=10.1093/hmg/ddp490;
RA   del Castillo F.J., Cohen-Salmon M., Charollais A., Caille D., Lampe P.D.,
RA   Chavrier P., Meda P., Petit C.;
RT   "Consortin, a trans-Golgi network cargo receptor for the plasma membrane
RT   targeting and recycling of connexins.";
RL   Hum. Mol. Genet. 19:262-275(2010).
CC   -!- FUNCTION: One gap junction consists of a cluster of closely packed
CC       pairs of transmembrane channels, the connexons, through which materials
CC       of low MW diffuse from one cell to a neighboring cell.
CC   -!- SUBUNIT: A connexon is composed of a hexamer of connexins. Interacts
CC       with CNST. {ECO:0000269|PubMed:19864490}.
CC   -!- INTERACTION:
CC       P70689; P39447: Tjp1; NbExp=3; IntAct=EBI-2615416, EBI-79508;
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Cell
CC       junction, gap junction.
CC   -!- TISSUE SPECIFICITY: Highly expressed in adult brain and skin. Less in
CC       uterus, lung and eye. Very low in testis and sciatic nerve. No
CC       expression before birth.
CC   -!- SIMILARITY: Belongs to the connexin family. Beta-type (group I)
CC       subfamily. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; Z70023; CAA93845.1; -; Genomic_DNA.
DR   EMBL; BC013811; AAH13811.1; -; mRNA.
DR   EMBL; BC016507; AAH16507.1; -; mRNA.
DR   EMBL; BC063762; AAH63762.1; -; mRNA.
DR   CCDS; CCDS27154.1; -.
DR   RefSeq; NP_001010937.1; NM_001010937.2.
DR   RefSeq; NP_001258592.1; NM_001271663.1.
DR   RefSeq; XP_006518623.1; XM_006518560.2.
DR   RefSeq; XP_006518624.1; XM_006518561.3.
DR   AlphaFoldDB; P70689; -.
DR   SMR; P70689; -.
DR   BioGRID; 199936; 4.
DR   IntAct; P70689; 2.
DR   MINT; P70689; -.
DR   STRING; 10090.ENSMUSP00000035630; -.
DR   GlyGen; P70689; 3 sites, 1 O-linked glycan (3 sites).
DR   iPTMnet; P70689; -.
DR   PhosphoSitePlus; P70689; -.
DR   PaxDb; 10090-ENSMUSP00000035630; -.
DR   ProteomicsDB; 279219; -.
DR   Antibodypedia; 6442; 282 antibodies from 29 providers.
DR   DNASU; 14623; -.
DR   Ensembl; ENSMUST00000039380.9; ENSMUSP00000035630.9; ENSMUSG00000040055.10.
DR   GeneID; 14623; -.
DR   KEGG; mmu:14623; -.
DR   UCSC; uc007ucy.2; mouse.
DR   AGR; MGI:107588; -.
DR   CTD; 10804; -.
DR   MGI; MGI:107588; Gjb6.
DR   VEuPathDB; HostDB:ENSMUSG00000040055; -.
DR   eggNOG; ENOG502QWM8; Eukaryota.
DR   GeneTree; ENSGT01030000234513; -.
DR   HOGENOM; CLU_037388_4_1_1; -.
DR   InParanoid; P70689; -.
DR   OMA; PNHAIKE; -.
DR   OrthoDB; 5301774at2759; -.
DR   PhylomeDB; P70689; -.
DR   TreeFam; TF329606; -.
DR   Reactome; R-MMU-190861; Gap junction assembly.
DR   BioGRID-ORCS; 14623; 0 hits in 78 CRISPR screens.
DR   PRO; PR:P70689; -.
DR   Proteomes; UP000000589; Chromosome 14.
DR   RNAct; P70689; Protein.
DR   Bgee; ENSMUSG00000040055; Expressed in utricle of membranous labyrinth and 139 other cell types or tissues.
DR   ExpressionAtlas; P70689; baseline and differential.
DR   GO; GO:0005884; C:actin filament; ISO:MGI.
DR   GO; GO:0016324; C:apical plasma membrane; ISO:MGI.
DR   GO; GO:0030054; C:cell junction; ISO:MGI.
DR   GO; GO:0005922; C:connexin complex; IBA:GO_Central.
DR   GO; GO:0005921; C:gap junction; IDA:MGI.
DR   GO; GO:0051015; F:actin filament binding; ISO:MGI.
DR   GO; GO:0048487; F:beta-tubulin binding; ISO:MGI.
DR   GO; GO:0005243; F:gap junction channel activity; ISO:MGI.
DR   GO; GO:1903763; F:gap junction channel activity involved in cell communication by electrical coupling; ISO:MGI.
DR   GO; GO:0008017; F:microtubule binding; ISO:MGI.
DR   GO; GO:0007267; P:cell-cell signaling; IBA:GO_Central.
DR   GO; GO:0071333; P:cellular response to glucose stimulus; IEA:Ensembl.
DR   GO; GO:0042471; P:ear morphogenesis; IMP:MGI.
DR   GO; GO:0016264; P:gap junction assembly; ISO:MGI.
DR   GO; GO:1990349; P:gap junction-mediated intercellular transport; ISO:MGI.
DR   GO; GO:0048839; P:inner ear development; IEA:Ensembl.
DR   GO; GO:0008285; P:negative regulation of cell population proliferation; ISO:MGI.
DR   GO; GO:0051602; P:response to electrical stimulus; IEA:Ensembl.
DR   GO; GO:0032496; P:response to lipopolysaccharide; IEA:Ensembl.
DR   GO; GO:0007605; P:sensory perception of sound; IMP:MGI.
DR   GO; GO:0055085; P:transmembrane transport; ISO:MGI.
DR   Gene3D; 1.20.1440.80; Gap junction channel protein cysteine-rich domain; 1.
DR   InterPro; IPR000500; Connexin.
DR   InterPro; IPR019570; Connexin_CCC.
DR   InterPro; IPR017990; Connexin_CS.
DR   InterPro; IPR013092; Connexin_N.
DR   InterPro; IPR038359; Connexin_N_sf.
DR   PANTHER; PTHR11984; CONNEXIN; 1.
DR   PANTHER; PTHR11984:SF23; GAP JUNCTION BETA-6 PROTEIN; 1.
DR   Pfam; PF00029; Connexin; 1.
DR   PRINTS; PR00206; CONNEXIN.
DR   SMART; SM00037; CNX; 1.
DR   SMART; SM01089; Connexin_CCC; 1.
DR   PROSITE; PS00407; CONNEXINS_1; 1.
DR   PROSITE; PS00408; CONNEXINS_2; 1.
DR   Genevisible; P70689; MM.
PE   1: Evidence at protein level;
KW   Cell junction; Cell membrane; Gap junction; Hearing; Membrane;
KW   Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..261
FT                   /note="Gap junction beta-6 protein"
FT                   /id="PRO_0000057872"
FT   TOPO_DOM        1..22
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        23..45
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        46..75
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        76..98
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        99..131
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        132..154
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        155..192
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        193..215
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        216..261
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   261 AA;  30366 MW;  AC6D8AA234AA47A6 CRC64;
     MDWGTLHTVI GGVNKHSTSI GKVWITVIFI FRVMILVVAA QEVWGDEQED FVCNTLQPGC
     KNVCYDHFFP VSHIRLWALQ LIFVSTPALL VAMHVAYYRH ETARKFIRGE KRNEFKDLED
     IKRQKVRIEG SLWWTYTSSI FFRIIFEAAF MYVFYFLYNG YHLPWVLKCG IDPCPNLVDC
     FISRPTEKTV FTVFMISASV ICMLLNVAEL CYLLLKLCFR RSKRTQAQRN HPNHALKESK
     QNEMNELISD SGQNAITSFP S
//