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Protein

Steroid 17-alpha-hydroxylase/17,20 lyase

Gene

CYP17A1

Organism
Mesocricetus auratus (Golden hamster)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. Involved in sexual development during fetal life and at puberty.

Catalytic activityi

A C(21)-steroid + (reduced NADPH--hemoprotein reductase) + O2 = a 17-alpha-hydroxy-C(21)-steroid + (oxidized NADPH--hemoprotein reductase) + H2O.
17-alpha-hydroxyprogesterone = androst-4-ene-3,17-dione + acetaldehyde.

Cofactori

hemeBy similarity

Enzyme regulationi

Regulated predominantly by intracellular cAMP levels.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi442 – 4421Iron (heme axial ligand)By similarity

GO - Molecular functioni

  1. 17-alpha-hydroxyprogesterone aldolase activity Source: UniProtKB-EC
  2. heme binding Source: UniProtKB
  3. iron ion binding Source: InterPro
  4. steroid 17-alpha-monooxygenase activity Source: UniProtKB

GO - Biological processi

  1. hormone biosynthetic process Source: UniProtKB
  2. progesterone metabolic process Source: UniProtKB
  3. steroid biosynthetic process Source: UniProtKB-UniPathway
  4. steroid metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Lyase, Monooxygenase, Oxidoreductase

Keywords - Biological processi

Steroidogenesis

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00062.

Names & Taxonomyi

Protein namesi
Recommended name:
Steroid 17-alpha-hydroxylase/17,20 lyase (EC:1.14.99.9, EC:4.1.2.30)
Alternative name(s):
17-alpha-hydroxyprogesterone aldolase
CYPXVII
Cytochrome P450 17A1
Cytochrome P450-C17
Short name:
Cytochrome P450c17
Gene namesi
Name:CYP17A1
Synonyms:CYP17
OrganismiMesocricetus auratus (Golden hamster)
Taxonomic identifieri10036 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeMesocricetus

Subcellular locationi

Membrane Curated

GO - Cellular componenti

  1. membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 511511Steroid 17-alpha-hydroxylase/17,20 lyasePRO_0000051934Add
BLAST

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1JO9model-A48-501[»]
ProteinModelPortaliP70687.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

HOVERGENiHBG106944.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P70687-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWELVALLLL TLAYFFWSKS KTCGAKSPKS LPFLPLVGSL PFIPRHGHPH
60 70 80 90 100
VNFFKLQEKY GPIYSLRLGS TTTVIIGQYQ LAKEVLVKKG KEFSGRPHMV
110 120 130 140 150
TLGLLSDQGK GIAFADSGGS WQLHRKLALS SFALFRDGNQ KLEKIICQKA
160 170 180 190 200
SSLCDFLLTH NEESIDLSEP IFNSITNIIC IICFGISYEN RDPILATIKS
210 220 230 240 250
FTEGILNSLG NDHLVDIFPW LTIFPNKTVD MIKKNVKIRD EVLSGILEKC
260 270 280 290 300
KEKFNSDSIS SLMDLLIQAK TNADNNNTSE GQGSNAFSDM HILATIADIF
310 320 330 340 350
GAGIETTASV LSWIIAFLLH NPEVKKKIQK EIDQNIGFSR TPTFNDRNHL
360 370 380 390 400
LMLEATIREV LRIRPVAPML IPHRANSDMS IGEFSIPKFT PVIINLWALH
410 420 430 440 450
HSEKEWDQPD RFMPERFLDP TGSHLITPSL SYLPFGAGAR SCIGEVLARQ
460 470 480 490 500
ELFLFMAHLL QRFDLDVPDD EQPPCLKGNA NVVFLIDPFK VKITVRQAWK
510
DAQAEVNTWR P
Length:511
Mass (Da):57,459
Last modified:February 1, 1997 - v1
Checksum:i896E776A7457546C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U66494 mRNA. Translation: AAB07494.1.
RefSeqiNP_001268860.1. NM_001281931.1.

Genome annotation databases

GeneIDi101831170.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U66494 mRNA. Translation: AAB07494.1.
RefSeqiNP_001268860.1. NM_001281931.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1JO9model-A48-501[»]
ProteinModelPortaliP70687.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi101831170.

Organism-specific databases

CTDi1586.

Phylogenomic databases

HOVERGENiHBG106944.

Enzyme and pathway databases

UniPathwayiUPA00062.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and expression of hamster adrenal cytochrome P450C17 cDNA."
    Cloutier M., Fleury A., Courtemanche J., Ducharme L., Mason J.I., Lehoux J.-G.
    Ann. N. Y. Acad. Sci. 774:294-296(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Adrenal gland.

Entry informationi

Entry nameiCP17A_MESAU
AccessioniPrimary (citable) accession number: P70687
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: January 7, 2015
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.