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P70683 (PAFA_CAVPO) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Platelet-activating factor acetylhydrolase

Short name=PAF acetylhydrolase
EC=3.1.1.47
Alternative name(s):
1-alkyl-2-acetylglycerophosphocholine esterase
2-acetyl-1-alkylglycerophosphocholine esterase
LDL-associated phospholipase A2
Short name=LDL-PLA(2)
PAF 2-acylhydrolase
Gene names
Name:PLA2G7
Synonyms:PAFAH
OrganismCavia porcellus (Guinea pig) [Reference proteome]
Taxonomic identifier10141 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaHystricognathiCaviidaeCavia

Protein attributes

Sequence length436 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Modulates the action of platelet-activating factor (PAF) by hydrolyzing the sn-2 ester bond to yield the biologically inactive lyso-PAF. Has a specificity for substrates with a short residue at the sn-2 position. It is inactive against long-chain phospholipids.

Catalytic activity

1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = 1-alkyl-sn-glycero-3-phosphocholine + acetate.

Subcellular location

Secretedextracellular space.

Tissue specificity

Plasma.

Sequence similarities

Belongs to the AB hydrolase superfamily. Lipase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 By similarity
Chain22 – 436415Platelet-activating factor acetylhydrolase
PRO_0000017832

Sites

Active site2711Nucleophile By similarity
Active site2941Charge relay system By similarity
Active site3491Charge relay system By similarity

Amino acid modifications

Glycosylation761N-linked (GlcNAc...) Potential
Glycosylation2001N-linked (GlcNAc...) Potential
Glycosylation3241N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
P70683 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: C359D96E392FFE11

FASTA43649,063
        10         20         30         40         50         60 
MAPPKLHTLF CLSGFLALVH PFDWRDLDPV TYIQSSVWIQ RIQSELLITS FGHTTIPKGN 

        70         80         90        100        110        120 
GPYSVGCTDL MSGYTNQSSF LRLYYPSQDN DFPDALWIPN EEYFQGLTET LGASSFLGKL 

       130        140        150        160        170        180 
LKLLYGSVKV PAKWNSPLKT GEKYPLIIFS HGLGAFRSIY SAIGIELASH GFIVAAVEHR 

       190        200        210        220        230        240 
DESAAATYYF QDAPAAESGN RSWIYYKVGN LETEERKRQL RQRGEECSQA LSWLLSIDEG 

       250        260        270        280        290        300 
EPVKNVLDLN FDIQQLKGSL DRSKVAIIGH SFGGATVIQT LSEDQRFRCG IALDPWMFPV 

       310        320        330        340        350        360 
GEDVHSKIPQ PLFFINSEYF QSANDTKKIE KFYQPQKERK MIAVKGSVHH NFVDFTFATG 

       370        380        390        400        410        420 
KIIGQMLSLK GKIDSEVAMD LINKASLAFL QKYLGLDKNF DQWNSLMEGD DENLIPEFTI 

       430 
PTTMQSSTGT EQRNPD 

« Hide

References

[1]"Cloning, expression and characterization of plasma platelet-activating factor-acetylhydrolase from guinea pig."
Karasawa K., Kuge O., Kawasaki K., Nishijima M., Nakano Y., Tomita M., Yokoyama K., Setaka M., Nojima S.
J. Biochem. 120:838-844(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Hartley.
Tissue: Liver.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D67037 mRNA. Translation: BAA11054.1.
PIRJC5021.
RefSeqNP_001166357.1. NM_001172886.1.
XP_005005344.1. XM_005005287.1.

3D structure databases

ProteinModelPortalP70683.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10141.ENSCPOP00000011506.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSCPOT00000012911; ENSCPOP00000011506; ENSCPOG00000012786.
GeneID100135600.

Organism-specific databases

CTD7941.

Phylogenomic databases

eggNOGCOG4188.
GeneTreeENSGT00390000005233.
HOGENOMHOG000008053.
HOVERGENHBG001322.
InParanoidP70683.
OMAKEYFFGL.
OrthoDBEOG7MSMQ5.
TreeFamTF313831.

Family and domain databases

InterProIPR005065. PAF_acetylhydro.
IPR016715. PAF_acetylhydro_eukaryote.
[Graphical view]
PANTHERPTHR10272. PTHR10272. 1 hit.
PfamPF03403. PAF-AH_p_II. 1 hit.
[Graphical view]
PIRSFPIRSF018169. PAF_acetylhydrolase. 1 hit.
PROSITEPS00120. LIPASE_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePAFA_CAVPO
AccessionPrimary (citable) accession number: P70683
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: February 19, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families