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P70683

- PAFA_CAVPO

UniProt

P70683 - PAFA_CAVPO

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Protein

Platelet-activating factor acetylhydrolase

Gene

PLA2G7

Organism
Cavia porcellus (Guinea pig)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Modulates the action of platelet-activating factor (PAF) by hydrolyzing the sn-2 ester bond to yield the biologically inactive lyso-PAF. Has a specificity for substrates with a short residue at the sn-2 position. It is inactive against long-chain phospholipids.

Catalytic activityi

1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = 1-alkyl-sn-glycero-3-phosphocholine + acetate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei271 – 2711NucleophileBy similarity
Active sitei294 – 2941Charge relay systemPROSITE-ProRule annotation
Active sitei349 – 3491Charge relay systemPROSITE-ProRule annotation

GO - Molecular functioni

  1. 1-alkyl-2-acetylglycerophosphocholine esterase activity Source: UniProtKB-EC
  2. calcium-independent phospholipase A2 activity Source: Ensembl
  3. phospholipid binding Source: Ensembl

GO - Biological processi

  1. lipid catabolic process Source: UniProtKB-KW
  2. lipid oxidation Source: Ensembl
  3. low-density lipoprotein particle remodeling Source: Ensembl
  4. plasma lipoprotein particle oxidation Source: Ensembl
  5. positive regulation of monocyte chemotaxis Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Platelet-activating factor acetylhydrolase (EC:3.1.1.47)
Short name:
PAF acetylhydrolase
Alternative name(s):
1-alkyl-2-acetylglycerophosphocholine esterase
2-acetyl-1-alkylglycerophosphocholine esterase
LDL-associated phospholipase A2
Short name:
LDL-PLA(2)
PAF 2-acylhydrolase
Gene namesi
Name:PLA2G7
Synonyms:PAFAH
OrganismiCavia porcellus (Guinea pig)
Taxonomic identifieri10141 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaHystricognathiCaviidaeCavia
ProteomesiUP000005447: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. low-density lipoprotein particle Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121By similarityAdd
BLAST
Chaini22 – 436415Platelet-activating factor acetylhydrolasePRO_0000017832Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi76 – 761N-linked (GlcNAc...)Sequence Analysis
Glycosylationi200 – 2001N-linked (GlcNAc...)Sequence Analysis
Glycosylationi324 – 3241N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Expressioni

Tissue specificityi

Plasma.

Interactioni

Protein-protein interaction databases

STRINGi10141.ENSCPOP00000011506.

Structurei

3D structure databases

ProteinModelPortaliP70683.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AB hydrolase superfamily. Lipase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG4188.
GeneTreeiENSGT00390000005233.
HOGENOMiHOG000008053.
HOVERGENiHBG001322.
InParanoidiP70683.
OMAiKEYFFGL.
OrthoDBiEOG7MSMQ5.
TreeFamiTF313831.

Family and domain databases

Gene3Di3.40.50.1820. 3 hits.
InterProiIPR029058. AB_hydrolase.
IPR005065. PAF_acetylhydro.
IPR016715. PAF_acetylhydro_eukaryote.
[Graphical view]
PANTHERiPTHR10272. PTHR10272. 1 hit.
PfamiPF03403. PAF-AH_p_II. 1 hit.
[Graphical view]
PIRSFiPIRSF018169. PAF_acetylhydrolase. 1 hit.
SUPFAMiSSF53474. SSF53474. 3 hits.
PROSITEiPS00120. LIPASE_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P70683-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAPPKLHTLF CLSGFLALVH PFDWRDLDPV TYIQSSVWIQ RIQSELLITS
60 70 80 90 100
FGHTTIPKGN GPYSVGCTDL MSGYTNQSSF LRLYYPSQDN DFPDALWIPN
110 120 130 140 150
EEYFQGLTET LGASSFLGKL LKLLYGSVKV PAKWNSPLKT GEKYPLIIFS
160 170 180 190 200
HGLGAFRSIY SAIGIELASH GFIVAAVEHR DESAAATYYF QDAPAAESGN
210 220 230 240 250
RSWIYYKVGN LETEERKRQL RQRGEECSQA LSWLLSIDEG EPVKNVLDLN
260 270 280 290 300
FDIQQLKGSL DRSKVAIIGH SFGGATVIQT LSEDQRFRCG IALDPWMFPV
310 320 330 340 350
GEDVHSKIPQ PLFFINSEYF QSANDTKKIE KFYQPQKERK MIAVKGSVHH
360 370 380 390 400
NFVDFTFATG KIIGQMLSLK GKIDSEVAMD LINKASLAFL QKYLGLDKNF
410 420 430
DQWNSLMEGD DENLIPEFTI PTTMQSSTGT EQRNPD
Length:436
Mass (Da):49,063
Last modified:February 1, 1997 - v1
Checksum:iC359D96E392FFE11
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D67037 mRNA. Translation: BAA11054.1.
PIRiJC5021.
RefSeqiNP_001166357.1. NM_001172886.1.
XP_005005344.1. XM_005005287.1.

Genome annotation databases

EnsembliENSCPOT00000012911; ENSCPOP00000011506; ENSCPOG00000012786.
GeneIDi100135600.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D67037 mRNA. Translation: BAA11054.1 .
PIRi JC5021.
RefSeqi NP_001166357.1. NM_001172886.1.
XP_005005344.1. XM_005005287.1.

3D structure databases

ProteinModelPortali P70683.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10141.ENSCPOP00000011506.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSCPOT00000012911 ; ENSCPOP00000011506 ; ENSCPOG00000012786 .
GeneIDi 100135600.

Organism-specific databases

CTDi 7941.

Phylogenomic databases

eggNOGi COG4188.
GeneTreei ENSGT00390000005233.
HOGENOMi HOG000008053.
HOVERGENi HBG001322.
InParanoidi P70683.
OMAi KEYFFGL.
OrthoDBi EOG7MSMQ5.
TreeFami TF313831.

Family and domain databases

Gene3Di 3.40.50.1820. 3 hits.
InterProi IPR029058. AB_hydrolase.
IPR005065. PAF_acetylhydro.
IPR016715. PAF_acetylhydro_eukaryote.
[Graphical view ]
PANTHERi PTHR10272. PTHR10272. 1 hit.
Pfami PF03403. PAF-AH_p_II. 1 hit.
[Graphical view ]
PIRSFi PIRSF018169. PAF_acetylhydrolase. 1 hit.
SUPFAMi SSF53474. SSF53474. 3 hits.
PROSITEi PS00120. LIPASE_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning, expression and characterization of plasma platelet-activating factor-acetylhydrolase from guinea pig."
    Karasawa K., Kuge O., Kawasaki K., Nishijima M., Nakano Y., Tomita M., Yokoyama K., Setaka M., Nojima S.
    J. Biochem. 120:838-844(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Hartley.
    Tissue: Liver.

Entry informationi

Entry nameiPAFA_CAVPO
AccessioniPrimary (citable) accession number: P70683
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: October 1, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3