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P70683

- PAFA_CAVPO

UniProt

P70683 - PAFA_CAVPO

Protein

Platelet-activating factor acetylhydrolase

Gene

PLA2G7

Organism
Cavia porcellus (Guinea pig)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
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    Functioni

    Modulates the action of platelet-activating factor (PAF) by hydrolyzing the sn-2 ester bond to yield the biologically inactive lyso-PAF. Has a specificity for substrates with a short residue at the sn-2 position. It is inactive against long-chain phospholipids.

    Catalytic activityi

    1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = 1-alkyl-sn-glycero-3-phosphocholine + acetate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei271 – 2711NucleophileBy similarity
    Active sitei294 – 2941Charge relay systemPROSITE-ProRule annotation
    Active sitei349 – 3491Charge relay systemPROSITE-ProRule annotation

    GO - Molecular functioni

    1. 1-alkyl-2-acetylglycerophosphocholine esterase activity Source: UniProtKB-EC
    2. calcium-independent phospholipase A2 activity Source: Ensembl
    3. phospholipid binding Source: Ensembl

    GO - Biological processi

    1. lipid catabolic process Source: UniProtKB-KW
    2. lipid oxidation Source: Ensembl
    3. low-density lipoprotein particle remodeling Source: Ensembl
    4. plasma lipoprotein particle oxidation Source: Ensembl
    5. positive regulation of monocyte chemotaxis Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Lipid degradation, Lipid metabolism

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Platelet-activating factor acetylhydrolase (EC:3.1.1.47)
    Short name:
    PAF acetylhydrolase
    Alternative name(s):
    1-alkyl-2-acetylglycerophosphocholine esterase
    2-acetyl-1-alkylglycerophosphocholine esterase
    LDL-associated phospholipase A2
    Short name:
    LDL-PLA(2)
    PAF 2-acylhydrolase
    Gene namesi
    Name:PLA2G7
    Synonyms:PAFAH
    OrganismiCavia porcellus (Guinea pig)
    Taxonomic identifieri10141 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaHystricognathiCaviidaeCavia
    ProteomesiUP000005447: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. low-density lipoprotein particle Source: Ensembl

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2121By similarityAdd
    BLAST
    Chaini22 – 436415Platelet-activating factor acetylhydrolasePRO_0000017832Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi76 – 761N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi200 – 2001N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi324 – 3241N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Expressioni

    Tissue specificityi

    Plasma.

    Interactioni

    Protein-protein interaction databases

    STRINGi10141.ENSCPOP00000011506.

    Structurei

    3D structure databases

    ProteinModelPortaliP70683.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the AB hydrolase superfamily. Lipase family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG4188.
    GeneTreeiENSGT00390000005233.
    HOGENOMiHOG000008053.
    HOVERGENiHBG001322.
    InParanoidiP70683.
    OMAiKEYFFGL.
    OrthoDBiEOG7MSMQ5.
    TreeFamiTF313831.

    Family and domain databases

    Gene3Di3.40.50.1820. 3 hits.
    InterProiIPR029058. AB_hydrolase.
    IPR005065. PAF_acetylhydro.
    IPR016715. PAF_acetylhydro_eukaryote.
    [Graphical view]
    PANTHERiPTHR10272. PTHR10272. 1 hit.
    PfamiPF03403. PAF-AH_p_II. 1 hit.
    [Graphical view]
    PIRSFiPIRSF018169. PAF_acetylhydrolase. 1 hit.
    SUPFAMiSSF53474. SSF53474. 3 hits.
    PROSITEiPS00120. LIPASE_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P70683-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAPPKLHTLF CLSGFLALVH PFDWRDLDPV TYIQSSVWIQ RIQSELLITS    50
    FGHTTIPKGN GPYSVGCTDL MSGYTNQSSF LRLYYPSQDN DFPDALWIPN 100
    EEYFQGLTET LGASSFLGKL LKLLYGSVKV PAKWNSPLKT GEKYPLIIFS 150
    HGLGAFRSIY SAIGIELASH GFIVAAVEHR DESAAATYYF QDAPAAESGN 200
    RSWIYYKVGN LETEERKRQL RQRGEECSQA LSWLLSIDEG EPVKNVLDLN 250
    FDIQQLKGSL DRSKVAIIGH SFGGATVIQT LSEDQRFRCG IALDPWMFPV 300
    GEDVHSKIPQ PLFFINSEYF QSANDTKKIE KFYQPQKERK MIAVKGSVHH 350
    NFVDFTFATG KIIGQMLSLK GKIDSEVAMD LINKASLAFL QKYLGLDKNF 400
    DQWNSLMEGD DENLIPEFTI PTTMQSSTGT EQRNPD 436
    Length:436
    Mass (Da):49,063
    Last modified:February 1, 1997 - v1
    Checksum:iC359D96E392FFE11
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D67037 mRNA. Translation: BAA11054.1.
    PIRiJC5021.
    RefSeqiNP_001166357.1. NM_001172886.1.
    XP_005005344.1. XM_005005287.1.

    Genome annotation databases

    EnsembliENSCPOT00000012911; ENSCPOP00000011506; ENSCPOG00000012786.
    GeneIDi100135600.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D67037 mRNA. Translation: BAA11054.1 .
    PIRi JC5021.
    RefSeqi NP_001166357.1. NM_001172886.1.
    XP_005005344.1. XM_005005287.1.

    3D structure databases

    ProteinModelPortali P70683.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10141.ENSCPOP00000011506.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSCPOT00000012911 ; ENSCPOP00000011506 ; ENSCPOG00000012786 .
    GeneIDi 100135600.

    Organism-specific databases

    CTDi 7941.

    Phylogenomic databases

    eggNOGi COG4188.
    GeneTreei ENSGT00390000005233.
    HOGENOMi HOG000008053.
    HOVERGENi HBG001322.
    InParanoidi P70683.
    OMAi KEYFFGL.
    OrthoDBi EOG7MSMQ5.
    TreeFami TF313831.

    Family and domain databases

    Gene3Di 3.40.50.1820. 3 hits.
    InterProi IPR029058. AB_hydrolase.
    IPR005065. PAF_acetylhydro.
    IPR016715. PAF_acetylhydro_eukaryote.
    [Graphical view ]
    PANTHERi PTHR10272. PTHR10272. 1 hit.
    Pfami PF03403. PAF-AH_p_II. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF018169. PAF_acetylhydrolase. 1 hit.
    SUPFAMi SSF53474. SSF53474. 3 hits.
    PROSITEi PS00120. LIPASE_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, expression and characterization of plasma platelet-activating factor-acetylhydrolase from guinea pig."
      Karasawa K., Kuge O., Kawasaki K., Nishijima M., Nakano Y., Tomita M., Yokoyama K., Setaka M., Nojima S.
      J. Biochem. 120:838-844(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Hartley.
      Tissue: Liver.

    Entry informationi

    Entry nameiPAFA_CAVPO
    AccessioniPrimary (citable) accession number: P70683
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 88 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3