Reviewed, UniProtKB/Swiss-Prot P70673 (IRK11_RAT)

Names and origin

Protein names

Recommended name:
    ATP-sensitive inward rectifier potassium channel 11
Alternative name(s):
    Potassium channel, inwardly rectifying subfamily J member 11
    Inward rectifier K(+) channel Kir6.2
    BIR

Gene names

Name:

Kcnj11

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	390 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	This receptor is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium By similarity.
Subunit structure	Associates with SUR.
Subcellular location	Membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the inward rectifier-type potassium channel family.

Ontologies

Keywords
Biological process	Ion transport Potassium transport Transport
Cellular component	Membrane
Domain	Transmembrane
Ligand	Potassium
Molecular function	Ionic channel Voltage-gated channel
Gene Ontology (GO)
Biological process	response to drug Inferred from expression pattern. Source: RGD response to testosterone stimulus Inferred from expression pattern. Source: RGD
Cellular component	ATP-sensitive potassium channel complex Inferred from direct assay. Source: RGD T-tubule Inferred from direct assay. Source: RGD endoplasmic reticulum Inferred from direct assay. Source: RGD microsome Inferred from direct assay. Source: RGD mitochondrion Inferred from direct assay. Source: RGD
Molecular function	ATP-activated inward rectifier potassium channel activity Inferred from direct assay. Source: RGD potassium ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein C-terminus binding Inferred from physical interaction. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 390	390	ATP-sensitive inward rectifier potassium channel 11		PRO_0000154960
Regions
	Topological domain	1 – 68	68	Cytoplasmic By similarity
	Transmembrane	69 – 93	25	M1 By similarity
	Topological domain	94 – 116	23	Extracellular By similarity
	Topological domain	136 – 144	9	Extracellular By similarity
	Transmembrane	145 – 166	22	M2 By similarity
	Topological domain	167 – 390	224	Cytoplasmic By similarity
	Region	117 – 128	12	H5 (pore-forming helix) By similarity
	Motif	130 – 135	6	Selectivity filter By similarity
Sites
	Site	160	1	Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesium By similarity
Experimental info
	Sequence conflict	191	1	L → P in BAA96239. Ref.5
	Sequence conflict	365	1	R → Q in AAB01810. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P70673-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: C8E59AD298DE4F13	FASTA	390	43,608
	10 20 30 40 50 60 MLSRKGIIPE EYVLTRLAED PTEPRYRTRE RRARFVSKKG NCNVAHKNIR EQGRFLQDVF 70 80 90 100 110 120 TTLVDLKWPH TLLIFTMSFL CSWLLFAMVW WLIAFAHGDL APGEGTNVPC VTSIHSFSSA 130 140 150 160 170 180 FLFSIEVQVT IGFGGRMVTE ECPLAILILI VQNIVGLMIN AIMLGCIFMK TAQAHRRAET 190 200 210 220 230 240 LIFSKHAVIT LRHGRLCFML RVGDLRKSMI ISATIHMQVV RKTTSPEGEV VPLHQVDIPM 250 260 270 280 290 300 ENGVGGNSIF LVAPLIIYHV IDSNSPLYDL APSDLHHHQD LEIIVILEGV VETTGITTQA 310 320 330 340 350 360 RTSYLADEIL WGQRFVPIVA EEDGRYSVDY SKFGNTVKVP TPLCTARQLD EDRSLLDALT 370 380 390 LASSRGPLRK RSVAVAKAKP KFSISPDSLS

References

[1]	"A novel sulfonylurea receptor forms with BIR (Kir6.2) a smooth muscle type ATP-sensitive K+ channel." Isomoto S., Kondo C., Yamada M., Matsumoto S., Higashiguchi O., Horio Y., Matsuzawa Y., Kurachi Y. J. Biol. Chem. 271:24321-24324(1996) [PubMed: 8798681] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	Faure C., Partiseti M., Gouhier C., Graham D. Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Pancreatic islet.
[3]	"Rat inwardly rectifying potassium channel Kir6.2: cloning electrophysiological characterization, and decreased expression in pancreatic islets of male Zucker diabetic fatty rats." Tokuyama Y., Fan Z., Furuta H., Makielski J.C., Polonsky K.S., Bell G.I., Yano H. Biochem. Biophys. Res. Commun. 220:532-538(1996) [PubMed: 8607800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar. Tissue: Pancreatic islet.
[4]	Wada Y., Takao K., Nishi M., Takeshima H., Imai K., Yamashita T., Kokubun S., Yoshii M., Nukada T. Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar. Tissue: Heart ventricle.
[5]	Cao K., Wang R. Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Vascular smooth muscle.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D86039 mRNA. Translation: BAA12971.1. X97041 mRNA. Translation: CAA65754.1. U44897 mRNA. Translation: AAB01810.1. D61687 mRNA. Translation: BAA23630.1. AB043638 mRNA. Translation: BAA96239.1.
IPI	IPI00190663.
PIR	JC4689.
RefSeq	NP_112648.2.
UniGene	Rn.3985
3D structure databases
HSSP	HSSP built from PDB template 1N9P based on UniProtKB P35562.
ModBase	Search...
Protein-protein interaction databases
STRING	P70673.
Protein family/group databases
TCDB	1.A.2.1.7. inward rectifier K+ channel (IRK-C) family.
PTM databases
PhosphoSite	P70673.
Proteomic databases
PRIDE	P70673.
Genome annotation databases
Ensembl	ENSRNOT00000028685; ENSRNOP00000028685; ENSRNOG00000021128; Rattus norvegicus. [Genome view]
GeneID	83535.
KEGG	rno:83535.
UCSC	NM_031358. rat.
Organism-specific databases
CTD	83535.
RGD	69247. Kcnj11.
Phylogenomic databases
HOVERGEN	P70673.
OMA	WLIAFAH.
Gene expression databases
ArrayExpress	P70673.
Genevestigator	P70673.
GermOnline	ENSRNOG00000021128. Rattus norvegicus.
Family and domain databases
InterPro	IPR016449. K_chnl_inward-rec_Kir. IPR001838. K_chnl_inward-rec_Kir-like. IPR003279. K_chnl_inward-rec_Kir6.2. IPR013521. K_chnl_inward-rec_Kir_Cr2. IPR013518. K_chnl_inward-rec_Kir_cyto. [Graphical view]
Gene3D	G3DSA:2.60.40.1400. IR_K+channel_cytopl. 1 hit.
PANTHER	PTHR11767. K+channel_IR. 1 hit.
Pfam	PF01007. IRK. 1 hit. [Graphical view]
PIRSF	PIRSF005465. GIRK_kir. 1 hit.
PRINTS	PR01332. KIR62CHANNEL. PR01320. KIRCHANNEL.
ProDom	PD001103. K+channel_IR. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...
Other Resources
NextBio	616041.

Entry information

Entry name

IRK11_RAT

Accession

Primary (citable) accession number: P70673
Secondary accession number(s): Q62906, Q9JM48

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	November 3, 2009
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)