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P70673 (IRK11_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ATP-sensitive inward rectifier potassium channel 11
Alternative name(s):
BIR
Inward rectifier K(+) channel Kir6.2
Potassium channel, inwardly rectifying subfamily J member 11
Gene names
Name:Kcnj11
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length390 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

This receptor is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium. Can form cardiac and smooth muscle-type KATP channels with ABCC9. KCNJ11 forms the channel pore while ABCC9 is required for activation and regulation By similarity.

Subunit structure

Interacts with ABCC9/SUR2 By similarity. Interacts with ABCC8/SUR.

Subcellular location

Membrane; Multi-pass membrane protein.

Post-translational modification

Phosphorylation by MAPK1 results in changes in channel gating that destabilize the closed states and reduce the ATP sensitivity By similarity.

Sequence similarities

Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ11 subfamily. [View classification]

Ontologies

Keywords
   Biological processIon transport
Potassium transport
Transport
   Cellular componentMembrane
   DomainTransmembrane
Transmembrane helix
   LigandPotassium
   Molecular functionIon channel
Voltage-gated channel
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcellular response to glucose stimulus

Inferred from expression pattern PubMed 19720793. Source: RGD

cellular response to nicotine

Inferred from expression pattern PubMed 22403787. Source: RGD

cellular response to tumor necrosis factor

Inferred from expression pattern PubMed 19460779. Source: RGD

glucose metabolic process

Inferred from electronic annotation. Source: Ensembl

negative regulation of insulin secretion

Inferred from electronic annotation. Source: Ensembl

neurological system process

Inferred from electronic annotation. Source: Ensembl

positive regulation of cation channel activity

Inferred from mutant phenotype PubMed 23785408. Source: RGD

potassium ion import

Inferred from electronic annotation. Source: Ensembl

potassium ion transport

Inferred from direct assay PubMed 17097686. Source: RGD

regulation of membrane potential

Inferred from electronic annotation. Source: Ensembl

response to ATP

Inferred from electronic annotation. Source: Ensembl

response to drug

Inferred from expression pattern PubMed 15964031. Source: RGD

response to estradiol

Inferred from expression pattern PubMed 23032400. Source: RGD

response to ischemia

Inferred from expression pattern PubMed 20971764. Source: RGD

response to testosterone

Inferred from expression pattern PubMed 18950632. Source: RGD

   Cellular_componentATP-sensitive potassium channel complex

Inferred from direct assay PubMed 17097686. Source: RGD

T-tubule

Inferred from direct assay PubMed 15647111. Source: RGD

axolemma

Inferred from direct assay PubMed 20102598. Source: RGD

cell body fiber

Inferred from direct assay PubMed 20102598. Source: RGD

cytosol

Inferred from direct assay PubMed 20102598. Source: RGD

endoplasmic reticulum

Inferred from direct assay PubMed 15983113. Source: RGD

endosome

Inferred from direct assay PubMed 20971764. Source: RGD

intercalated disc

Inferred from direct assay PubMed 23066018. Source: RGD

intracellular membrane-bounded organelle

Inferred from direct assay PubMed 15983113. Source: RGD

mitochondrion

Inferred from direct assay PubMed 15983113PubMed 16050978. Source: RGD

myelin sheath

Inferred from direct assay PubMed 20102598. Source: RGD

neuronal cell body

Inferred from direct assay PubMed 20102598. Source: RGD

nuclear envelope

Inferred from direct assay PubMed 20102598. Source: RGD

plasma membrane

Inferred from direct assay PubMed 15983113. Source: RGD

sarcolemma

Inferred from direct assay PubMed 12738227PubMed 20971764. Source: RGD

voltage-gated potassium channel complex

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionATP binding

Inferred from electronic annotation. Source: Ensembl

ATP-activated inward rectifier potassium channel activity

Inferred from direct assay PubMed 17097686. Source: RGD

ankyrin binding

Inferred from physical interaction PubMed 19805355. Source: BHF-UCL

heat shock protein binding

Inferred from physical interaction PubMed 20427569. Source: RGD

protein C-terminus binding

Inferred from physical interaction PubMed 14672537. Source: RGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 390390ATP-sensitive inward rectifier potassium channel 11
PRO_0000154960

Regions

Topological domain1 – 6868Cytoplasmic By similarity
Transmembrane69 – 9325Helical; Name=M1; By similarity
Topological domain94 – 11623Extracellular By similarity
Intramembrane117 – 12812Helical; Pore-forming; Name=H5; By similarity
Intramembrane129 – 1357Pore-forming; By similarity
Topological domain136 – 1449Extracellular By similarity
Transmembrane145 – 16622Helical; Name=M2; By similarity
Topological domain167 – 390224Cytoplasmic By similarity
Motif130 – 1356Selectivity filter By similarity

Sites

Site1601Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesium By similarity

Amino acid modifications

Modified residue3411Phosphothreonine; by MAPK1 By similarity
Modified residue3851Phosphoserine; by MAPK1 By similarity

Experimental info

Sequence conflict1911L → P in BAA96239. Ref.5
Sequence conflict3651R → Q in AAB01810. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P70673 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: C8E59AD298DE4F13

FASTA39043,608
        10         20         30         40         50         60 
MLSRKGIIPE EYVLTRLAED PTEPRYRTRE RRARFVSKKG NCNVAHKNIR EQGRFLQDVF 

        70         80         90        100        110        120 
TTLVDLKWPH TLLIFTMSFL CSWLLFAMVW WLIAFAHGDL APGEGTNVPC VTSIHSFSSA 

       130        140        150        160        170        180 
FLFSIEVQVT IGFGGRMVTE ECPLAILILI VQNIVGLMIN AIMLGCIFMK TAQAHRRAET 

       190        200        210        220        230        240 
LIFSKHAVIT LRHGRLCFML RVGDLRKSMI ISATIHMQVV RKTTSPEGEV VPLHQVDIPM 

       250        260        270        280        290        300 
ENGVGGNSIF LVAPLIIYHV IDSNSPLYDL APSDLHHHQD LEIIVILEGV VETTGITTQA 

       310        320        330        340        350        360 
RTSYLADEIL WGQRFVPIVA EEDGRYSVDY SKFGNTVKVP TPLCTARQLD EDRSLLDALT 

       370        380        390 
LASSRGPLRK RSVAVAKAKP KFSISPDSLS 

« Hide

References

[1]"A novel sulfonylurea receptor forms with BIR (Kir6.2) a smooth muscle type ATP-sensitive K+ channel."
Isomoto S., Kondo C., Yamada M., Matsumoto S., Higashiguchi O., Horio Y., Matsuzawa Y., Kurachi Y.
J. Biol. Chem. 271:24321-24324(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]Faure C., Partiseti M., Gouhier C., Graham D.
Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Pancreatic islet.
[3]"Rat inwardly rectifying potassium channel Kir6.2: cloning electrophysiological characterization, and decreased expression in pancreatic islets of male Zucker diabetic fatty rats."
Tokuyama Y., Fan Z., Furuta H., Makielski J.C., Polonsky K.S., Bell G.I., Yano H.
Biochem. Biophys. Res. Commun. 220:532-538(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Wistar.
Tissue: Pancreatic islet.
[4]Wada Y., Takao K., Nishi M., Takeshima H., Imai K., Yamashita T., Kokubun S., Yoshii M., Nukada T.
Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Wistar.
Tissue: Heart ventricle.
[5]Cao K., Wang R.
Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Vascular smooth muscle.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D86039 mRNA. Translation: BAA12971.1.
X97041 mRNA. Translation: CAA65754.1.
U44897 mRNA. Translation: AAB01810.1.
D61687 mRNA. Translation: BAA23630.1.
AB043638 mRNA. Translation: BAA96239.1.
PIRJC4689.
RefSeqNP_112648.2. NM_031358.3.
UniGeneRn.3985.

3D structure databases

ProteinModelPortalP70673.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-58641N.

Chemistry

BindingDBP70673.
GuidetoPHARMACOLOGY442.

Protein family/group databases

TCDB1.A.2.1.7. inward rectifier k(+) channel (irk-c) family.

PTM databases

PhosphoSiteP70673.

Proteomic databases

PRIDEP70673.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000028685; ENSRNOP00000028685; ENSRNOG00000021128.
GeneID83535.
KEGGrno:83535.
UCSCRGD:69247. rat.

Organism-specific databases

CTD3767.
RGD69247. Kcnj11.

Phylogenomic databases

eggNOGNOG72812.
GeneTreeENSGT00690000102074.
HOGENOMHOG000237325.
HOVERGENHBG006178.
InParanoidP70673.
KOK05004.
OMAHHQDLEI.
OrthoDBEOG7XPZ5K.
PhylomeDBP70673.
TreeFamTF313676.

Gene expression databases

GenevestigatorP70673.

Family and domain databases

Gene3D2.60.40.1400. 1 hit.
InterProIPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR003279. K_chnl_inward-rec_Kir6.2.
IPR013518. K_chnl_inward-rec_Kir_cyto.
[Graphical view]
PANTHERPTHR11767. PTHR11767. 1 hit.
PfamPF01007. IRK. 1 hit.
[Graphical view]
PIRSFPIRSF005465. GIRK_kir. 1 hit.
PRINTSPR01332. KIR62CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMSSF81296. SSF81296. 1 hit.
ProtoNetSearch...

Other

NextBio616041.
PROP70673.

Entry information

Entry nameIRK11_RAT
AccessionPrimary (citable) accession number: P70673
Secondary accession number(s): Q62906, Q9JM48
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: April 16, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families