Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

ATP-sensitive inward rectifier potassium channel 11

Gene

Kcnj11

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

This receptor is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium. Can form cardiac and smooth muscle-type KATP channels with ABCC9. KCNJ11 forms the channel pore while ABCC9 is required for activation and regulation (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei160 – 1601Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesiumBy similarity

GO - Molecular functioni

  1. ankyrin binding Source: BHF-UCL
  2. ATP-activated inward rectifier potassium channel activity Source: RGD
  3. ATP binding Source: Ensembl
  4. heat shock protein binding Source: RGD
  5. protein C-terminus binding Source: RGD

GO - Biological processi

  1. cellular response to glucose stimulus Source: RGD
  2. cellular response to nicotine Source: RGD
  3. cellular response to tumor necrosis factor Source: RGD
  4. glucose metabolic process Source: Ensembl
  5. negative regulation of insulin secretion Source: Ensembl
  6. neurological system process Source: Ensembl
  7. positive regulation of cation channel activity Source: RGD
  8. potassium ion import Source: GO_Central
  9. potassium ion transport Source: RGD
  10. regulation of membrane potential Source: Ensembl
  11. response to ATP Source: Ensembl
  12. response to drug Source: RGD
  13. response to estradiol Source: RGD
  14. response to ischemia Source: RGD
  15. response to testosterone Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Enzyme and pathway databases

ReactomeiREACT_302029. ATP sensitive Potassium channels.
REACT_345047. Regulation of insulin secretion.

Protein family/group databases

TCDBi1.A.2.1.7. inward rectifier k(+) channel (irk-c) family.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-sensitive inward rectifier potassium channel 11
Alternative name(s):
BIR
Inward rectifier K(+) channel Kir6.2
Potassium channel, inwardly rectifying subfamily J member 11
Gene namesi
Name:Kcnj11
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi69247. Kcnj11.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 6868CytoplasmicBy similarityAdd
BLAST
Transmembranei69 – 9325Helical; Name=M1By similarityAdd
BLAST
Topological domaini94 – 11623ExtracellularBy similarityAdd
BLAST
Intramembranei117 – 12812Helical; Pore-forming; Name=H5By similarityAdd
BLAST
Intramembranei129 – 1357Pore-formingBy similarity
Topological domaini136 – 1449ExtracellularBy similarity
Transmembranei145 – 16622Helical; Name=M2By similarityAdd
BLAST
Topological domaini167 – 390224CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  1. ATP-sensitive potassium channel complex Source: RGD
  2. axolemma Source: RGD
  3. cell body fiber Source: RGD
  4. cytosol Source: RGD
  5. endoplasmic reticulum Source: RGD
  6. endosome Source: RGD
  7. intercalated disc Source: RGD
  8. intracellular membrane-bounded organelle Source: RGD
  9. mitochondrion Source: RGD
  10. myelin sheath Source: RGD
  11. neuronal cell body Source: RGD
  12. nuclear envelope Source: RGD
  13. plasma membrane Source: RGD
  14. sarcolemma Source: RGD
  15. T-tubule Source: RGD
  16. voltage-gated potassium channel complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 390390ATP-sensitive inward rectifier potassium channel 11PRO_0000154960Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei341 – 3411Phosphothreonine; by MAPK1By similarity
Modified residuei385 – 3851Phosphoserine; by MAPK1By similarity

Post-translational modificationi

Phosphorylation by MAPK1 results in changes in channel gating that destabilize the closed states and reduce the ATP sensitivity.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP70673.

PTM databases

PhosphoSiteiP70673.

Expressioni

Gene expression databases

GenevestigatoriP70673.

Interactioni

Subunit structurei

Interacts with ABCC9/SUR2 (By similarity). Interacts with ABCC8/SUR.By similarity

Protein-protein interaction databases

DIPiDIP-58641N.

Structurei

3D structure databases

ProteinModelPortaliP70673.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi130 – 1356Selectivity filterBy similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG72812.
GeneTreeiENSGT00760000118842.
HOGENOMiHOG000237325.
HOVERGENiHBG006178.
InParanoidiP70673.
KOiK05004.
OMAiGNCNVAH.
OrthoDBiEOG7XPZ5K.
PhylomeDBiP70673.
TreeFamiTF313676.

Family and domain databases

Gene3Di2.60.40.1400. 1 hit.
InterProiIPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR003279. K_chnl_inward-rec_Kir6.2.
IPR013518. K_chnl_inward-rec_Kir_cyto.
[Graphical view]
PANTHERiPTHR11767. PTHR11767. 1 hit.
PTHR11767:SF44. PTHR11767:SF44. 1 hit.
PfamiPF01007. IRK. 1 hit.
[Graphical view]
PIRSFiPIRSF005465. GIRK_kir. 1 hit.
PRINTSiPR01332. KIR62CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMiSSF81296. SSF81296. 1 hit.

Sequencei

Sequence statusi: Complete.

P70673-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSRKGIIPE EYVLTRLAED PTEPRYRTRE RRARFVSKKG NCNVAHKNIR
60 70 80 90 100
EQGRFLQDVF TTLVDLKWPH TLLIFTMSFL CSWLLFAMVW WLIAFAHGDL
110 120 130 140 150
APGEGTNVPC VTSIHSFSSA FLFSIEVQVT IGFGGRMVTE ECPLAILILI
160 170 180 190 200
VQNIVGLMIN AIMLGCIFMK TAQAHRRAET LIFSKHAVIT LRHGRLCFML
210 220 230 240 250
RVGDLRKSMI ISATIHMQVV RKTTSPEGEV VPLHQVDIPM ENGVGGNSIF
260 270 280 290 300
LVAPLIIYHV IDSNSPLYDL APSDLHHHQD LEIIVILEGV VETTGITTQA
310 320 330 340 350
RTSYLADEIL WGQRFVPIVA EEDGRYSVDY SKFGNTVKVP TPLCTARQLD
360 370 380 390
EDRSLLDALT LASSRGPLRK RSVAVAKAKP KFSISPDSLS
Length:390
Mass (Da):43,608
Last modified:January 31, 1997 - v1
Checksum:iC8E59AD298DE4F13
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti191 – 1911L → P in BAA96239 (Ref. 5) Curated
Sequence conflicti365 – 3651R → Q in AAB01810 (PubMed:8607800).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D86039 mRNA. Translation: BAA12971.1.
X97041 mRNA. Translation: CAA65754.1.
U44897 mRNA. Translation: AAB01810.1.
D61687 mRNA. Translation: BAA23630.1.
AB043638 mRNA. Translation: BAA96239.1.
PIRiJC4689.
RefSeqiNP_112648.2. NM_031358.3.
UniGeneiRn.3985.

Genome annotation databases

EnsembliENSRNOT00000028685; ENSRNOP00000028685; ENSRNOG00000021128.
GeneIDi83535.
KEGGirno:83535.
UCSCiRGD:69247. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D86039 mRNA. Translation: BAA12971.1.
X97041 mRNA. Translation: CAA65754.1.
U44897 mRNA. Translation: AAB01810.1.
D61687 mRNA. Translation: BAA23630.1.
AB043638 mRNA. Translation: BAA96239.1.
PIRiJC4689.
RefSeqiNP_112648.2. NM_031358.3.
UniGeneiRn.3985.

3D structure databases

ProteinModelPortaliP70673.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-58641N.

Chemistry

ChEMBLiCHEMBL3038487.
GuidetoPHARMACOLOGYi442.

Protein family/group databases

TCDBi1.A.2.1.7. inward rectifier k(+) channel (irk-c) family.

PTM databases

PhosphoSiteiP70673.

Proteomic databases

PRIDEiP70673.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000028685; ENSRNOP00000028685; ENSRNOG00000021128.
GeneIDi83535.
KEGGirno:83535.
UCSCiRGD:69247. rat.

Organism-specific databases

CTDi3767.
RGDi69247. Kcnj11.

Phylogenomic databases

eggNOGiNOG72812.
GeneTreeiENSGT00760000118842.
HOGENOMiHOG000237325.
HOVERGENiHBG006178.
InParanoidiP70673.
KOiK05004.
OMAiGNCNVAH.
OrthoDBiEOG7XPZ5K.
PhylomeDBiP70673.
TreeFamiTF313676.

Enzyme and pathway databases

ReactomeiREACT_302029. ATP sensitive Potassium channels.
REACT_345047. Regulation of insulin secretion.

Miscellaneous databases

NextBioi616041.
PROiP70673.

Gene expression databases

GenevestigatoriP70673.

Family and domain databases

Gene3Di2.60.40.1400. 1 hit.
InterProiIPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR003279. K_chnl_inward-rec_Kir6.2.
IPR013518. K_chnl_inward-rec_Kir_cyto.
[Graphical view]
PANTHERiPTHR11767. PTHR11767. 1 hit.
PTHR11767:SF44. PTHR11767:SF44. 1 hit.
PfamiPF01007. IRK. 1 hit.
[Graphical view]
PIRSFiPIRSF005465. GIRK_kir. 1 hit.
PRINTSiPR01332. KIR62CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMiSSF81296. SSF81296. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "A novel sulfonylurea receptor forms with BIR (Kir6.2) a smooth muscle type ATP-sensitive K+ channel."
    Isomoto S., Kondo C., Yamada M., Matsumoto S., Higashiguchi O., Horio Y., Matsuzawa Y., Kurachi Y.
    J. Biol. Chem. 271:24321-24324(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Faure C., Partiseti M., Gouhier C., Graham D.
    Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Pancreatic islet.
  3. "Rat inwardly rectifying potassium channel Kir6.2: cloning electrophysiological characterization, and decreased expression in pancreatic islets of male Zucker diabetic fatty rats."
    Tokuyama Y., Fan Z., Furuta H., Makielski J.C., Polonsky K.S., Bell G.I., Yano H.
    Biochem. Biophys. Res. Commun. 220:532-538(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
    Tissue: Pancreatic islet.
  4. Wada Y., Takao K., Nishi M., Takeshima H., Imai K., Yamashita T., Kokubun S., Yoshii M., Nukada T.
    Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
    Tissue: Heart ventricle.
  5. Cao K., Wang R.
    Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Vascular smooth muscle.

Entry informationi

Entry nameiKCJ11_RAT
AccessioniPrimary (citable) accession number: P70673
Secondary accession number(s): Q62906, Q9JM48
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 1997
Last sequence update: January 31, 1997
Last modified: March 31, 2015
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.