Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Nascent polypeptide-associated complex subunit alpha, muscle-specific form

Gene

Naca

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cardiac- and muscle-specific transcription factor. May act to regulate the expression of genes involved in the development of myotubes. Plays a critical role in ventricular cardiomyocyte expansion and regulates postnatal skeletal muscle growth and regeneration. Involved in the organized assembly of thick and thin filaments of myofibril sarcomeres.3 Publications

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • TBP-class protein binding Source: MGI
  • transcription coactivator activity Source: MGI

GO - Biological processi

  • myoblast migration Source: MGI
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW
  • transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation, Transport

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Nascent polypeptide-associated complex subunit alpha, muscle-specific form
Alternative name(s):
Alpha-NAC, muscle-specific form
Short name:
skNAC
Gene namesi
Name:Naca
Synonyms:Gm1878
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:106095. Naca.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • extracellular exosome Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Partial embryonic lethality by embryonic day 12.5, with ventricular hypoplasia and decreased cardiomyocyte proliferation. Viable skNAC(-/-) adult mice have reduced postnatal skeletal muscle growth and impaired regenerative capacity after cardiotoxin-induced injury. Satellite cells have impaired survival compared with wild-type.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi1952 – 19521L → A: Loss of interaction with SMYD1. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 21872187Nascent polypeptide-associated complex subunit alpha, muscle-specific formPRO_0000135578Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei590 – 5901PhosphothreonineCombined sources
Modified residuei822 – 8221PhosphoserineCombined sources
Modified residuei1174 – 11741PhosphoserineCombined sources
Modified residuei1177 – 11771PhosphoserineCombined sources
Modified residuei1364 – 13641PhosphothreonineCombined sources
Modified residuei1368 – 13681PhosphoserineCombined sources
Modified residuei1392 – 13921PhosphoserineCombined sources
Modified residuei1398 – 13981PhosphothreonineCombined sources
Modified residuei1400 – 14001PhosphoserineCombined sources
Modified residuei1423 – 14231PhosphoserineCombined sources
Modified residuei1492 – 14921PhosphoserineCombined sources
Modified residuei2015 – 20151Phosphoserine; by ILK1Curated
Modified residuei2114 – 21141N6-acetyllysineCombined sources
Modified residuei2131 – 21311Phosphothreonine; by GSK3-betaCombined sourcesCurated
Modified residuei2133 – 21331PhosphothreonineBy similarity
Modified residuei2138 – 21381PhosphoserineCombined sources
Modified residuei2158 – 21581PhosphoserineBy similarity
Modified residuei2163 – 21631PhosphoserineBy similarity
Modified residuei2175 – 21751PhosphoserineBy similarity

Post-translational modificationi

Phosphorylation of Ser-2015 by ILK during cell adhesion may promote nuclear localization. Phosphorylation of Thr-2131 by GSK3B may promote proteasome mediated degradation.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP70670.
PaxDbiP70670.
PRIDEiP70670.

PTM databases

iPTMnetiP70670.
PhosphoSiteiP70670.

Expressioni

Tissue specificityi

Specifically expressed in heart and skeletal muscle: it is present in differentiated myotubes but not in myoblasts.1 Publication

Developmental stagei

Expressed concomitant with the onset of mineralization in ossification centers of developing bone.

Inductioni

Induced in muscle by wounding.

Gene expression databases

BgeeiP70670.
ExpressionAtlasiP70670. baseline.
GenevisibleiP70670. MM.

Interactioni

Subunit structurei

Interacts (via PXLXP motif) with the muscle-restricted histone methyltransferase SMYD1 (via MYND-type zinc finger).2 Publications

GO - Molecular functioni

  • TBP-class protein binding Source: MGI

Protein-protein interaction databases

BioGridi201682. 7 interactions.
IntActiP70670. 4 interactions.
MINTiMINT-1857727.
STRINGi10090.ENSMUSP00000089680.

Structurei

3D structure databases

ProteinModelPortaliP70670.
SMRiP70670. Positions 2051-2104.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2042 – 210766NAC-A/BPROSITE-ProRule annotationAdd
BLAST
Domaini2148 – 218538UBAAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2041 – 205212Required for DNA-bindingAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi1950 – 19545PXLXP

Sequence similaritiesi

Belongs to the NAC-alpha family.Curated
Contains 1 NAC-A/B (NAC-alpha/beta) domain.PROSITE-ProRule annotation
Contains 1 UBA domain.Curated

Phylogenomic databases

eggNOGiKOG2239. Eukaryota.
COG1308. LUCA.
GeneTreeiENSGT00440000033468.
HOGENOMiHOG000168224.
InParanoidiP70670.
KOiK03626.
OMAiFPSHLEN.
OrthoDBiEOG7RNK24.
TreeFamiTF313348.

Family and domain databases

InterProiIPR016641. EGD2/NACA.
IPR002715. Nas_poly-pep-assoc_cplx_dom.
[Graphical view]
PANTHERiPTHR21713. PTHR21713. 15 hits.
PfamiPF01849. NAC. 1 hit.
[Graphical view]
SMARTiSM01407. NAC. 1 hit.
[Graphical view]
PROSITEiPS51151. NAC_AB. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 2 (identifier: P70670-1) [UniParc]FASTAAdd to basket

Also known as: Gp220, skNAC

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPGEATETVP ATEQELPQPQ AETAVLPMSS ALKVAAVGQP GPTPPSSLGP
60 70 80 90 100
QQSPIVTAHQ PSPLPSSVSS TPFEVPFAQP ITAETALPSG TAPPTPTFLP
110 120 130 140 150
HLIGPPISPA ALALASPMIG LAQKGARSSS APLSLVALAP HSVQKSSVCP
160 170 180 190 200
PHPLTSPPSA AGAELGALTA SIPPLEPKTS TSQVPSQGTL NLKGTAPCPP
210 220 230 240 250
DVVRAFPSHL ENPLASVQPG LMSCPQTLSN TSPVKGVPIS SALTQSRLSL
260 270 280 290 300
NLKGPVSPPA RNTAAPSIPL APSTSLGCHL PLLHHSSVDS PIQPPGQSGL
310 320 330 340 350
AVSNPTSVGH SGIAASCPPE RCVVPALPSR LLAVDSGAAP SDDKGSSAVT
360 370 380 390 400
NELCSPPGSS NVAGTSLSPK ASLVPKGSNV ALQPLVTQVP ASQKTGLKEI
410 420 430 440 450
PVSCIGATHH ALDNPSAISV APATHVPPPT SSGLVSSKDP ASPVTSLVVP
460 470 480 490 500
AAHKQFPAPP ASATLGVPVS PLPATEGLKN LPISALVNVG APVSPAQAGL
510 520 530 540 550
PTRKDTTLQP LAPIALKESP SSQSASSLEV LSEDTVTKKT TGGPAPVVRP
560 570 580 590 600
AIAGVATTTS LRADSPPAVI RADSCVSPNT VSQPLKRSVT DPAMAPRTAK
610 620 630 640 650
NTAPSTTSPL VPLASEGCPV ASSMALSPQN ASVSETALAL SPEIPKSVPF
660 670 680 690 700
PDPPLAEISF SNARKVDAVS HMESSGSSRQ GHPDASVTAK GTVVCLADSS
710 720 730 740 750
LDTSVSASKG SALSGASSPL YPLEVSFLPE AGLAVQGPKG SLNKLSPTPP
760 770 780 790 800
SSKGAPVPST GAPPSPKGAP IVPTESSISS KQVPAEILPS PQKTPEVTAS
810 820 830 840 850
RLISAVQSPK VDPIMSDVTP TSPKKTSATA VPKDTSATLS LKSVPAVTSL
860 870 880 890 900
SPPKAPVAPS NEATIVPTEI PTSLKNALAA ATPKETLATS IPKVTSPSPQ
910 920 930 940 950
KTPKSVSLKG APAMTSKKAT EIAASKDVSP SQFPKEVPLL PHVPPTSPPK
960 970 980 990 1000
SPVSDTLSGA LTSPPPKGPP ATLAETPTYP KKSPKPAASK KTPATPSPEG
1010 1020 1030 1040 1050
VTAVPLEIPP CSKKAPKTAA PKESSATSSS KRAPKTAVSK EIPSKGVTAV
1060 1070 1080 1090 1100
PLEISLPLKE TSKSATPGEK SASSPKRSPK TAGPKETPPG GVTAVPPEIS
1110 1120 1130 1140 1150
LPPKETPQNA TPNESLAASS QKRSPKTSVP KETPPGGVTA MPLEIPSAPQ
1160 1170 1180 1190 1200
KAPKTAVPKQ IPTPEDAVTI LAGSPLSPKK ASKTAAPKEA PATPSVGVIA
1210 1220 1230 1240 1250
VSGEISPSPK KTSKTAAPKE NSATLPPKRS PKTAAPKETP ATSSEGVTAV
1260 1270 1280 1290 1300
PSEISPSPPT PASKGVPVTL TPKGAPNALA ESPASPKKVP KTAAPEETST
1310 1320 1330 1340 1350
TPSPQKIPKV AGPKEASATP PSKKTPKTAV PKETSAPSEG VTAVPLEIPP
1360 1370 1380 1390 1400
SPRKAPKTAA PKETPAPSPE GATTAPVQIP PSPRKGSKKA GSKETPTTPS
1410 1420 1430 1440 1450
PEGVTAAPLE IPISSKKTSK MASPKETLVT PSSKKLSQTV GPKETSLEGA
1460 1470 1480 1490 1500
TAVPLEIPPS HKKAPKTVDP KQVPLTPSPK DAPTTLAESP SSPKKAPKTA
1510 1520 1530 1540 1550
APPSERVTTV PPEKPATPQK ASATTASKVP VPAETQEVAV SSRETPVTPA
1560 1570 1580 1590 1600
VPPVKNPSSH KKTSKTIELK EAPATLPPSP TKSPKIPSSK KAPRTSAPKE
1610 1620 1630 1640 1650
FPASPSIKPV TTSLAQTAPP SLQKAPSTTI PKENLAAPAV LPVSSKSPAA
1660 1670 1680 1690 1700
PAAASASLSP ATAAPQTAPK EATTIPSCKK AAATETPIET STAPSLEGAP
1710 1720 1730 1740 1750
KETSETSVSK VLMSSPPKKA SSSKRASTLP ATTLPSLKEA SVLSPTATSS
1760 1770 1780 1790 1800
GKDSHISPVS DACSTGTTTP QASEKLPSKK GPTAFTEMLA APAPESALAI
1810 1820 1830 1840 1850
TAPIQKSPGA NSNSASSPKC PDPSSKKDTK GLPSAVALAP QTVPVEKDTS
1860 1870 1880 1890 1900
KAIETLLVSP AKGSDCLHSP KGPVGSQVAT PLAAFTSDKV PPEAVSASVA
1910 1920 1930 1940 1950
PKPAPAASLT LAPSPVAPLP PKQPLLESAP GSVLESPSKL PVPAEEDELP
1960 1970 1980 1990 2000
PLIPPEAVSG GEPFQPILVN MPAPKPAGTP APAPSAKQPV LKNNKGSGTE
2010 2020 2030 2040 2050
SDSDESVPEL EEQDSTQTAT QQAQLAAAAE IDEEPVSKAK QSRSEKKARK
2060 2070 2080 2090 2100
AMSKLGLRQV TGVTRVTIRK SKNILFVITK PDVYKSPASD TYIVFGEAKI
2110 2120 2130 2140 2150
EDLSQQAQLA AAEKFKVQGE AVSNIQENTQ TPTVQEESEE EEVDETGVEV
2160 2170 2180
KDIELVMSQA NVSRAKAVRA LKNNSNDIVN AIMELTM
Length:2,187
Mass (Da):220,499
Last modified:July 27, 2011 - v2
Checksum:i7BB6EDE3538F0954
GO
Isoform 1 (identifier: Q60817-1) [UniParc]FASTAAdd to basket

The sequence of this isoform can be found in the external entry Q60817.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:215
Mass (Da):23,384
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti941 – 9411P → Q in AAB18734 (PubMed:8698236).Curated
Sequence conflicti941 – 9411P → Q in AAB18732 (PubMed:8698236).Curated
Sequence conflicti1523 – 15231A → G in AAB18734 (PubMed:8698236).Curated
Sequence conflicti1523 – 15231A → G in AAB18732 (PubMed:8698236).Curated
Sequence conflicti1653 – 16531A → R in AAB18734 (PubMed:8698236).Curated
Sequence conflicti1653 – 16531A → R in AAB18732 (PubMed:8698236).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U48364 mRNA. Translation: AAB18734.1.
U48363 Genomic DNA. Translation: AAB18732.1.
AC131120 Genomic DNA. No translation available.
CCDSiCCDS48723.1. [P70670-1]
PIRiT30826.
RefSeqiNP_001106670.1. NM_001113199.1. [P70670-1]
NP_038636.2. NM_013608.3.
UniGeneiMm.3746.

Genome annotation databases

EnsembliENSMUST00000092048; ENSMUSP00000089680; ENSMUSG00000061315. [P70670-1]
GeneIDi17938.
KEGGimmu:17938.
UCSCiuc007hlc.2. mouse. [P70670-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U48364 mRNA. Translation: AAB18734.1.
U48363 Genomic DNA. Translation: AAB18732.1.
AC131120 Genomic DNA. No translation available.
CCDSiCCDS48723.1. [P70670-1]
PIRiT30826.
RefSeqiNP_001106670.1. NM_001113199.1. [P70670-1]
NP_038636.2. NM_013608.3.
UniGeneiMm.3746.

3D structure databases

ProteinModelPortaliP70670.
SMRiP70670. Positions 2051-2104.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201682. 7 interactions.
IntActiP70670. 4 interactions.
MINTiMINT-1857727.
STRINGi10090.ENSMUSP00000089680.

PTM databases

iPTMnetiP70670.
PhosphoSiteiP70670.

Proteomic databases

EPDiP70670.
PaxDbiP70670.
PRIDEiP70670.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000092048; ENSMUSP00000089680; ENSMUSG00000061315. [P70670-1]
GeneIDi17938.
KEGGimmu:17938.
UCSCiuc007hlc.2. mouse. [P70670-1]

Organism-specific databases

CTDi4666.
MGIiMGI:106095. Naca.

Phylogenomic databases

eggNOGiKOG2239. Eukaryota.
COG1308. LUCA.
GeneTreeiENSGT00440000033468.
HOGENOMiHOG000168224.
InParanoidiP70670.
KOiK03626.
OMAiFPSHLEN.
OrthoDBiEOG7RNK24.
TreeFamiTF313348.

Miscellaneous databases

NextBioi292841.
SOURCEiSearch...

Gene expression databases

BgeeiP70670.
ExpressionAtlasiP70670. baseline.
GenevisibleiP70670. MM.

Family and domain databases

InterProiIPR016641. EGD2/NACA.
IPR002715. Nas_poly-pep-assoc_cplx_dom.
[Graphical view]
PANTHERiPTHR21713. PTHR21713. 15 hits.
PfamiPF01849. NAC. 1 hit.
[Graphical view]
SMARTiSM01407. NAC. 1 hit.
[Graphical view]
PROSITEiPS51151. NAC_AB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Differential splicing-in of a proline-rich exon converts alphaNAC into a muscle-specific transcription factor."
    Yotov W.V., St Arnaud R.
    Genes Dev. 10:1763-1772(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 2), FUNCTION, ALTERNATIVE SPLICING, TISSUE SPECIFICITY.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "m-Bop, a repressor protein essential for cardiogenesis, interacts with skNAC, a heart- and muscle-specific transcription factor."
    Sims R.J. III, Weihe E.K., Zhu L., O'Malley S., Harriss J.V., Gottlieb P.D.
    J. Biol. Chem. 277:26524-26529(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SMYD1, MUTAGENESIS OF LEU-1952, SUBCELLULAR LOCATION.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2138, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic brain.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2138, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  6. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2138, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-590; SER-822; SER-1174; SER-1177; THR-1364; SER-1368; SER-1392; THR-1398; SER-1400; SER-1423; SER-1492; THR-2131 AND SER-2138, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.
  8. "skNAC, a Smyd1-interacting transcription factor, is involved in cardiac development and skeletal muscle growth and regeneration."
    Park C.Y., Pierce S.A., von Drehle M., Ivey K.N., Morgan J.A., Blau H.M., Srivastava D.
    Proc. Natl. Acad. Sci. U.S.A. 107:20750-20755(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SMYD1, DISRUPTION PHENOTYPE.
  9. "skNAC depletion stimulates myoblast migration and perturbs sarcomerogenesis by enhancing calpain 1 and 3 activity."
    Berkholz J., Zakrzewicz A., Munz B.
    Biochem. J. 453:303-310(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-2114, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiNACAM_MOUSE
AccessioniPrimary (citable) accession number: P70670
Secondary accession number(s): E9QMB1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 27, 2011
Last modified: May 11, 2016
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.