P70670 (NACAM_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nascent polypeptide-associated complex subunit alpha, muscle-specific form

Alternative name(s):
Alpha-NAC, muscle-specific form

Gene names

Name:	Naca
Synonyms:	Gm1878

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	2187 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. Also reduces the inherent affinity of ribosomes for protein translocation sites in the ER membrane (M sites) By similarity. Isoform 1 and isoform 2 appear to bind DNA and play roles in transcription. May act to regulate the expression of genes involved in the development of myotubes. Ref.1
Subunit structure	Part of the nascent polypeptide-associated complex (NAC), consisting of NACA and BTF3. NAC associates with ribosomes through the BTF3 subunit. Both subunits can contact nascent polypeptide chains By similarity. Interacts with TBP and JUN.
Subcellular location	Cytoplasm. Nucleus.
Tissue specificity	Specifically expressed in heart and skeletal muscle: it is present in differentiated myotubes but not in myoblasts. Ref.1
Developmental stage	Expressed concomitant with the onset of mineralization in ossification centers of developing bone.
Induction	Induced in muscle by wounding.
Post-translational modification	Phosphorylation of Ser-2015 by ILK during cell adhesion may promote nuclear localization. Phosphorylation of Thr-2131 by GSK3B may promote proteasome mediated degradation.
Sequence similarities	Belongs to the NAC-alpha family. Contains 1 NAC-A/B (NAC-alpha/beta) domain. Contains 1 UBA domain.

Ontologies

Keywords
Biological process	Protein transport Transcription Transcription regulation Transport
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing
Ligand	DNA-binding
Molecular function	Activator
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein transport Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from direct assay PubMed 9488445. Source: MGI nucleus Inferred from direct assay PubMed 9488445. Source: MGI
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW TBP-class protein binding Inferred from direct assay PubMed 9488445. Source: MGI transcription coactivator activity Inferred from direct assay PubMed 9488445. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 2 (identifier: P70670-1)

Also known as: Gp220; skNAC;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 1 (identifier: Q60817-1)

The sequence of this isoform can be found in the external entry Q60817.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2187	2187	Nascent polypeptide-associated complex subunit alpha, muscle-specific form		PRO_0000135578
Regions
	Domain	2042 – 2107	66	NAC-A/B
	Domain	2148 – 2185	38	UBA
	Region	2041 – 2052	12	Required for DNA-binding
Amino acid modifications
	Modified residue	1024	1	Phosphoserine Ref.4
	Modified residue	2015	1	Phosphoserine; by ILK1 Probable
	Modified residue	2131	1	Phosphothreonine; by GSK3-beta Probable
	Modified residue	2133	1	Phosphothreonine Ref.3
	Modified residue	2138	1	Phosphoserine Ref.3
Experimental info
	Sequence conflict	941	1	P → Q in AAB18734. Ref.1
	Sequence conflict	941	1	P → Q in AAB18732. Ref.1
	Sequence conflict	1523	1	A → G in AAB18734. Ref.1
	Sequence conflict	1523	1	A → G in AAB18732. Ref.1
	Sequence conflict	1653	1	A → R in AAB18734. Ref.1
	Sequence conflict	1653	1	A → R in AAB18732. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 2 (Gp220) (skNAC) [UniParc]. Last modified July 27, 2011. Version 2. Checksum: 7BB6EDE3538F0954	FASTA	2,187	220,499
	10 20 30 40 50 60 MPGEATETVP ATEQELPQPQ AETAVLPMSS ALKVAAVGQP GPTPPSSLGP QQSPIVTAHQ 70 80 90 100 110 120 PSPLPSSVSS TPFEVPFAQP ITAETALPSG TAPPTPTFLP HLIGPPISPA ALALASPMIG 130 140 150 160 170 180 LAQKGARSSS APLSLVALAP HSVQKSSVCP PHPLTSPPSA AGAELGALTA SIPPLEPKTS 190 200 210 220 230 240 TSQVPSQGTL NLKGTAPCPP DVVRAFPSHL ENPLASVQPG LMSCPQTLSN TSPVKGVPIS 250 260 270 280 290 300 SALTQSRLSL NLKGPVSPPA RNTAAPSIPL APSTSLGCHL PLLHHSSVDS PIQPPGQSGL 310 320 330 340 350 360 AVSNPTSVGH SGIAASCPPE RCVVPALPSR LLAVDSGAAP SDDKGSSAVT NELCSPPGSS 370 380 390 400 410 420 NVAGTSLSPK ASLVPKGSNV ALQPLVTQVP ASQKTGLKEI PVSCIGATHH ALDNPSAISV 430 440 450 460 470 480 APATHVPPPT SSGLVSSKDP ASPVTSLVVP AAHKQFPAPP ASATLGVPVS PLPATEGLKN 490 500 510 520 530 540 LPISALVNVG APVSPAQAGL PTRKDTTLQP LAPIALKESP SSQSASSLEV LSEDTVTKKT 550 560 570 580 590 600 TGGPAPVVRP AIAGVATTTS LRADSPPAVI RADSCVSPNT VSQPLKRSVT DPAMAPRTAK 610 620 630 640 650 660 NTAPSTTSPL VPLASEGCPV ASSMALSPQN ASVSETALAL SPEIPKSVPF PDPPLAEISF 670 680 690 700 710 720 SNARKVDAVS HMESSGSSRQ GHPDASVTAK GTVVCLADSS LDTSVSASKG SALSGASSPL 730 740 750 760 770 780 YPLEVSFLPE AGLAVQGPKG SLNKLSPTPP SSKGAPVPST GAPPSPKGAP IVPTESSISS 790 800 810 820 830 840 KQVPAEILPS PQKTPEVTAS RLISAVQSPK VDPIMSDVTP TSPKKTSATA VPKDTSATLS 850 860 870 880 890 900 LKSVPAVTSL SPPKAPVAPS NEATIVPTEI PTSLKNALAA ATPKETLATS IPKVTSPSPQ 910 920 930 940 950 960 KTPKSVSLKG APAMTSKKAT EIAASKDVSP SQFPKEVPLL PHVPPTSPPK SPVSDTLSGA 970 980 990 1000 1010 1020 LTSPPPKGPP ATLAETPTYP KKSPKPAASK KTPATPSPEG VTAVPLEIPP CSKKAPKTAA 1030 1040 1050 1060 1070 1080 PKESSATSSS KRAPKTAVSK EIPSKGVTAV PLEISLPLKE TSKSATPGEK SASSPKRSPK 1090 1100 1110 1120 1130 1140 TAGPKETPPG GVTAVPPEIS LPPKETPQNA TPNESLAASS QKRSPKTSVP KETPPGGVTA 1150 1160 1170 1180 1190 1200 MPLEIPSAPQ KAPKTAVPKQ IPTPEDAVTI LAGSPLSPKK ASKTAAPKEA PATPSVGVIA 1210 1220 1230 1240 1250 1260 VSGEISPSPK KTSKTAAPKE NSATLPPKRS PKTAAPKETP ATSSEGVTAV PSEISPSPPT 1270 1280 1290 1300 1310 1320 PASKGVPVTL TPKGAPNALA ESPASPKKVP KTAAPEETST TPSPQKIPKV AGPKEASATP 1330 1340 1350 1360 1370 1380 PSKKTPKTAV PKETSAPSEG VTAVPLEIPP SPRKAPKTAA PKETPAPSPE GATTAPVQIP 1390 1400 1410 1420 1430 1440 PSPRKGSKKA GSKETPTTPS PEGVTAAPLE IPISSKKTSK MASPKETLVT PSSKKLSQTV 1450 1460 1470 1480 1490 1500 GPKETSLEGA TAVPLEIPPS HKKAPKTVDP KQVPLTPSPK DAPTTLAESP SSPKKAPKTA 1510 1520 1530 1540 1550 1560 APPSERVTTV PPEKPATPQK ASATTASKVP VPAETQEVAV SSRETPVTPA VPPVKNPSSH 1570 1580 1590 1600 1610 1620 KKTSKTIELK EAPATLPPSP TKSPKIPSSK KAPRTSAPKE FPASPSIKPV TTSLAQTAPP 1630 1640 1650 1660 1670 1680 SLQKAPSTTI PKENLAAPAV LPVSSKSPAA PAAASASLSP ATAAPQTAPK EATTIPSCKK 1690 1700 1710 1720 1730 1740 AAATETPIET STAPSLEGAP KETSETSVSK VLMSSPPKKA SSSKRASTLP ATTLPSLKEA 1750 1760 1770 1780 1790 1800 SVLSPTATSS GKDSHISPVS DACSTGTTTP QASEKLPSKK GPTAFTEMLA APAPESALAI 1810 1820 1830 1840 1850 1860 TAPIQKSPGA NSNSASSPKC PDPSSKKDTK GLPSAVALAP QTVPVEKDTS KAIETLLVSP 1870 1880 1890 1900 1910 1920 AKGSDCLHSP KGPVGSQVAT PLAAFTSDKV PPEAVSASVA PKPAPAASLT LAPSPVAPLP 1930 1940 1950 1960 1970 1980 PKQPLLESAP GSVLESPSKL PVPAEEDELP PLIPPEAVSG GEPFQPILVN MPAPKPAGTP 1990 2000 2010 2020 2030 2040 APAPSAKQPV LKNNKGSGTE SDSDESVPEL EEQDSTQTAT QQAQLAAAAE IDEEPVSKAK 2050 2060 2070 2080 2090 2100 QSRSEKKARK AMSKLGLRQV TGVTRVTIRK SKNILFVITK PDVYKSPASD TYIVFGEAKI 2110 2120 2130 2140 2150 2160 EDLSQQAQLA AAEKFKVQGE AVSNIQENTQ TPTVQEESEE EEVDETGVEV KDIELVMSQA 2170 2180 NVSRAKAVRA LKNNSNDIVN AIMELTM
	Isoform 1 [UniParc]. See Q60817.		–	–

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Differential splicing-in of a proline-rich exon converts alphaNAC into a muscle-specific transcription factor." Yotov W.V., St Arnaud R. Genes Dev. 10:1763-1772(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 2), FUNCTION, ALTERNATIVE SPLICING, TISSUE SPECIFICITY.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-2133 AND SER-2138, MASS SPECTROMETRY. Tissue: Liver.
[4]	"The phagosomal proteome in interferon-gamma-activated macrophages." Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P. Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1024, MASS SPECTROMETRY. Tissue: Macrophage.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U48364 mRNA. Translation: AAB18734.1. U48363 Genomic DNA. Translation: AAB18732.1. AC131120 Genomic DNA. No translation available.
IPI	IPI00111831.
PIR	T30826.
RefSeq	NP_001106670.1. NM_001113199.1. NP_038636.2. NM_013608.3.
UniGene	Mm.3746.
3D structure databases
ProteinModelPortal	P70670.
SMR	P70670. Positions 2051-2104.
ModBase	Search...
Protein-protein interaction databases
IntAct	P70670. 3 interactions.
PTM databases
PhosphoSite	P70670.
Proteomic databases
PaxDb	P70670.
PRIDE	P70670.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000092048; ENSMUSP00000089680; ENSMUSG00000061315.
GeneID	17938.
KEGG	mmu:17938.
UCSC	uc007hlc.2. mouse.
Organism-specific databases
CTD	4666.
MGI	MGI:106095. Naca.
Phylogenomic databases
eggNOG	COG1308.
GeneTree	ENSGT00440000033468.
HOGENOM	HOG000168224.
InParanoid	P70670.
KO	K03626.
OMA	KESQATP.
OrthoDB	EOG41RPW6.
Gene expression databases
ArrayExpress	P70670.
Bgee	P70670.
Genevestigator	P70670.
GermOnline	ENSMUSG00000061315. Mus musculus.
Family and domain databases
InterPro	IPR016641. EGD2/NACA. IPR002715. Nas_poly-pep-assoc_cplx_dom. [Graphical view]
PANTHER	PTHR21713. PTHR21713. 1 hit.
Pfam	PF01849. NAC. 1 hit. [Graphical view]
PROSITE	PS51151. NAC_AB. 1 hit. PS50030. UBA. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	292841.
SOURCE	Search...

Entry information

Entry name

NACAM_MOUSE

Accession

Primary (citable) accession number: P70670
Secondary accession number(s): E9QMB1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	July 27, 2011
Last modified:	May 1, 2013
This is version 99 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program