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P70669

- PHEX_MOUSE

UniProt

P70669 - PHEX_MOUSE

Protein

Metalloendopeptidase homolog PEX

Gene

Phex

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
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    Functioni

    Probably involved in bone and dentin mineralization and renal phosphate reabsorption.

    Cofactori

    Binds 1 zinc ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi580 – 5801Zinc; catalyticPROSITE-ProRule annotation
    Active sitei581 – 5811PROSITE-ProRule annotation
    Metal bindingi584 – 5841Zinc; catalyticPROSITE-ProRule annotation
    Metal bindingi642 – 6421Zinc; catalyticPROSITE-ProRule annotation
    Active sitei646 – 6461Proton donorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. metalloendopeptidase activity Source: InterPro

    GO - Biological processi

    1. bone mineralization Source: MGI
    2. organophosphate metabolic process Source: MGI

    Keywords - Molecular functioni

    Hydrolase, Metalloprotease, Protease

    Keywords - Biological processi

    Biomineralization

    Keywords - Ligandi

    Metal-binding, Zinc

    Protein family/group databases

    MEROPSiM13.091.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Metalloendopeptidase homolog PEX (EC:3.4.24.-)
    Alternative name(s):
    Phosphate regulating neutral endopeptidase
    Vitamin D-resistant hypophosphatemic rickets protein
    X-linked hypophosphatemia protein
    Short name:
    HYP
    Gene namesi
    Name:Phex
    Synonyms:Hyp, Pex
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome X

    Organism-specific databases

    MGIiMGI:107489. Phex.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum Source: MGI
    2. Golgi apparatus Source: MGI
    3. integral component of membrane Source: UniProtKB-KW
    4. perinuclear region of cytoplasm Source: MGI

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 749749Metalloendopeptidase homolog PEXPRO_0000078229Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi71 – 711N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi238 – 2381N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi263 – 2631N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi290 – 2901N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi301 – 3011N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi377 – 3771N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi484 – 4841N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi736 – 7361N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiP70669.
    PRIDEiP70669.

    PTM databases

    PhosphoSiteiP70669.

    Expressioni

    Tissue specificityi

    Bone.

    Gene expression databases

    ArrayExpressiP70669.
    BgeeiP70669.
    GenevestigatoriP70669.

    Structurei

    3D structure databases

    ProteinModelPortaliP70669.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 2020CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini38 – 749712ExtracellularSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei21 – 3717Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase M13 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG3590.
    HOGENOMiHOG000245574.
    HOVERGENiHBG005554.
    InParanoidiP70669.
    KOiK08636.
    OMAiKVGYPEF.
    PhylomeDBiP70669.
    TreeFamiTF315192.

    Family and domain databases

    Gene3Di3.40.390.10. 2 hits.
    InterProiIPR024079. MetalloPept_cat_dom.
    IPR000718. Peptidase_M13.
    IPR018497. Peptidase_M13_C.
    IPR008753. Peptidase_M13_N.
    [Graphical view]
    PANTHERiPTHR11733. PTHR11733. 1 hit.
    PfamiPF01431. Peptidase_M13. 1 hit.
    PF05649. Peptidase_M13_N. 1 hit.
    [Graphical view]
    PRINTSiPR00786. NEPRILYSIN.
    PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P70669-1 [UniParc]FASTAAdd to Basket

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    MEAETGSTME TGKGTNRGIR IALALFIGGT LVLGTLLFLV SQGLLSFQAK    50
    QEYCLKPECI EAAAAIMSKV NLSVDPCENF FRFACDGWIS NNPIPEDMPS 100
    YGVYPWLRHN VDLKLKALLE KSVSRRRDTE AVQKAKILYS SCMNEKAIEK 150
    ADAKPLLHIL RHSPFRWPVL EANIGPEGVW SERKFSLLQT LATFRGQYSN 200
    SVFIRLYVSP DDKASNEHIL KLDQATLSLA VREDFLDNTT EAKSYRDALY 250
    KFMVDTAVLL GANSSRAEHD MKSVLRLEIK IAEIMIPHEN RTSEAMYNKM 300
    NISELSAMIP QFDWLGYIKK VIDTRLYPHL KDIGPSENVV VRVPQYFKDL 350
    FRILGAERKK TIANYLVWRM VYSRIPNLSR RFQYRWLEFS RVIQGTTTLL 400
    PQWDKCVNFI ESALPYVVGK MFVNVHFQED KKEMMEELIE GVRWAFIDML 450
    EKENEWMDAG TKRKAQEKAR AVLAKVGYPE FIMNDTYVNE DLKAIKFSES 500
    DYFGNVLQTR KYLAQSDFFW LRKAVPKTEW FTNPTTVNAF YSASTNQIRF 550
    PAGELQKPFF WGTEYPRSLS YGAIGVIVGH EFTHGFDNNG RKYDKNGNLD 600
    PWWSVESEEK FKEKTKCMIN QYSNYYWKKA GLNVKGKRTL GENIADNGGL 650
    REAFRAYRKW INDRRQGVEE PLLPGITFTN NQLFFLSYAH VRCNSYRPEA 700
    AREQVQIGAH SPPQFRVNGA ISNFEEFQKA FNCPRNSTMN RGADSCRLW 749
    Length:749
    Mass (Da):86,419
    Last modified:February 1, 1997 - v1
    Checksum:iA88FA481C376C18A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti430 – 4301D → V in AAB47750. (PubMed:9077527)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U49908 mRNA. Translation: AAC36502.1.
    U73910 mRNA. Translation: AAC25962.1.
    U73912 Genomic DNA. Translation: AAC25964.1.
    U73913 Genomic DNA. Translation: AAC25965.1.
    U73914 Genomic DNA. Translation: AAC25966.1.
    U73911 Genomic DNA. Translation: AAC25963.1.
    U73915 Genomic DNA. Translation: AAC25967.1.
    U75646 mRNA. Translation: AAB47750.1.
    CCDSiCCDS30497.1.
    RefSeqiNP_035207.1. NM_011077.2.
    UniGeneiMm.2529.

    Genome annotation databases

    EnsembliENSMUST00000079945; ENSMUSP00000078863; ENSMUSG00000057457.
    GeneIDi18675.
    KEGGimmu:18675.
    UCSCiuc009ury.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U49908 mRNA. Translation: AAC36502.1 .
    U73910 mRNA. Translation: AAC25962.1 .
    U73912 Genomic DNA. Translation: AAC25964.1 .
    U73913 Genomic DNA. Translation: AAC25965.1 .
    U73914 Genomic DNA. Translation: AAC25966.1 .
    U73911 Genomic DNA. Translation: AAC25963.1 .
    U73915 Genomic DNA. Translation: AAC25967.1 .
    U75646 mRNA. Translation: AAB47750.1 .
    CCDSi CCDS30497.1.
    RefSeqi NP_035207.1. NM_011077.2.
    UniGenei Mm.2529.

    3D structure databases

    ProteinModelPortali P70669.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi M13.091.

    PTM databases

    PhosphoSitei P70669.

    Proteomic databases

    PaxDbi P70669.
    PRIDEi P70669.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000079945 ; ENSMUSP00000078863 ; ENSMUSG00000057457 .
    GeneIDi 18675.
    KEGGi mmu:18675.
    UCSCi uc009ury.1. mouse.

    Organism-specific databases

    CTDi 5251.
    MGIi MGI:107489. Phex.

    Phylogenomic databases

    eggNOGi COG3590.
    HOGENOMi HOG000245574.
    HOVERGENi HBG005554.
    InParanoidi P70669.
    KOi K08636.
    OMAi KVGYPEF.
    PhylomeDBi P70669.
    TreeFami TF315192.

    Miscellaneous databases

    NextBioi 294698.
    PROi P70669.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P70669.
    Bgeei P70669.
    Genevestigatori P70669.

    Family and domain databases

    Gene3Di 3.40.390.10. 2 hits.
    InterProi IPR024079. MetalloPept_cat_dom.
    IPR000718. Peptidase_M13.
    IPR018497. Peptidase_M13_C.
    IPR008753. Peptidase_M13_N.
    [Graphical view ]
    PANTHERi PTHR11733. PTHR11733. 1 hit.
    Pfami PF01431. Peptidase_M13. 1 hit.
    PF05649. Peptidase_M13_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00786. NEPRILYSIN.
    PROSITEi PS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "cDNA cloning of the murine Pex gene implicated in X-linked hypophosphatemia and evidence for expression in bone."
      Du L., Desbarats M., Viel J., Glorieux F.H., Cawthorn C., Ecarot B.
      Genomics 36:22-28(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Pex gene deletions in Gy and Hyp mice provide mouse models for X-linked hypophosphatemia."
      Strom T.M., Francis F., Lorenz B., Boeddrich A., Econs M.J., Lehrach H., Meitinger T.
      Hum. Mol. Genet. 6:165-171(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    3. "Pex/PEX tissue distribution and evidence for a deletion in the 3' region of the Pex gene in X-linked hypophosphatemic mice."
      Beck L., Soumounou Y., Martel J., Krishnamurthy G., Gauthier C., Goodyer C.G., Tenenhouse H.S.
      J. Clin. Invest. 99:1200-1209(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6J.

    Entry informationi

    Entry nameiPHEX_MOUSE
    AccessioniPrimary (citable) accession number: P70669
    Secondary accession number(s): P97439
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 115 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3