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Protein

Metalloendopeptidase homolog PEX

Gene

Phex

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Probably involved in bone and dentin mineralization and renal phosphate reabsorption.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi580 – 5801Zinc; catalyticPROSITE-ProRule annotation
Active sitei581 – 5811PROSITE-ProRule annotation
Metal bindingi584 – 5841Zinc; catalyticPROSITE-ProRule annotation
Metal bindingi642 – 6421Zinc; catalyticPROSITE-ProRule annotation
Active sitei646 – 6461Proton donorPROSITE-ProRule annotation

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. metalloendopeptidase activity Source: InterPro

GO - Biological processi

  1. bone mineralization Source: MGI
  2. organophosphate metabolic process Source: MGI
  3. proteolysis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Biological processi

Biomineralization

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM13.091.

Names & Taxonomyi

Protein namesi
Recommended name:
Metalloendopeptidase homolog PEX (EC:3.4.24.-)
Alternative name(s):
Phosphate regulating neutral endopeptidase
Vitamin D-resistant hypophosphatemic rickets protein
X-linked hypophosphatemia protein
Short name:
HYP
Gene namesi
Name:Phex
Synonyms:Hyp, Pex
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome X

Organism-specific databases

MGIiMGI:107489. Phex.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2020CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei21 – 3717Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini38 – 749712ExtracellularSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum Source: MGI
  2. Golgi apparatus Source: MGI
  3. integral component of membrane Source: UniProtKB-KW
  4. perinuclear region of cytoplasm Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 749749Metalloendopeptidase homolog PEXPRO_0000078229Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi71 – 711N-linked (GlcNAc...)Sequence Analysis
Glycosylationi238 – 2381N-linked (GlcNAc...)Sequence Analysis
Glycosylationi263 – 2631N-linked (GlcNAc...)Sequence Analysis
Glycosylationi290 – 2901N-linked (GlcNAc...)Sequence Analysis
Glycosylationi301 – 3011N-linked (GlcNAc...)Sequence Analysis
Glycosylationi377 – 3771N-linked (GlcNAc...)Sequence Analysis
Glycosylationi484 – 4841N-linked (GlcNAc...)Sequence Analysis
Glycosylationi736 – 7361N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP70669.
PRIDEiP70669.

PTM databases

PhosphoSiteiP70669.

Expressioni

Tissue specificityi

Bone.

Gene expression databases

BgeeiP70669.
ExpressionAtlasiP70669. baseline and differential.
GenevestigatoriP70669.

Structurei

3D structure databases

ProteinModelPortaliP70669.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M13 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG3590.
HOGENOMiHOG000245574.
HOVERGENiHBG005554.
InParanoidiP70669.
KOiK08636.
OMAiEFIMNDT.
PhylomeDBiP70669.
TreeFamiTF315192.

Family and domain databases

Gene3Di3.40.390.10. 2 hits.
InterProiIPR024079. MetalloPept_cat_dom.
IPR000718. Peptidase_M13.
IPR018497. Peptidase_M13_C.
IPR008753. Peptidase_M13_N.
[Graphical view]
PANTHERiPTHR11733. PTHR11733. 1 hit.
PfamiPF01431. Peptidase_M13. 1 hit.
PF05649. Peptidase_M13_N. 1 hit.
[Graphical view]
PRINTSiPR00786. NEPRILYSIN.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P70669-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEAETGSTME TGKGTNRGIR IALALFIGGT LVLGTLLFLV SQGLLSFQAK
60 70 80 90 100
QEYCLKPECI EAAAAIMSKV NLSVDPCENF FRFACDGWIS NNPIPEDMPS
110 120 130 140 150
YGVYPWLRHN VDLKLKALLE KSVSRRRDTE AVQKAKILYS SCMNEKAIEK
160 170 180 190 200
ADAKPLLHIL RHSPFRWPVL EANIGPEGVW SERKFSLLQT LATFRGQYSN
210 220 230 240 250
SVFIRLYVSP DDKASNEHIL KLDQATLSLA VREDFLDNTT EAKSYRDALY
260 270 280 290 300
KFMVDTAVLL GANSSRAEHD MKSVLRLEIK IAEIMIPHEN RTSEAMYNKM
310 320 330 340 350
NISELSAMIP QFDWLGYIKK VIDTRLYPHL KDIGPSENVV VRVPQYFKDL
360 370 380 390 400
FRILGAERKK TIANYLVWRM VYSRIPNLSR RFQYRWLEFS RVIQGTTTLL
410 420 430 440 450
PQWDKCVNFI ESALPYVVGK MFVNVHFQED KKEMMEELIE GVRWAFIDML
460 470 480 490 500
EKENEWMDAG TKRKAQEKAR AVLAKVGYPE FIMNDTYVNE DLKAIKFSES
510 520 530 540 550
DYFGNVLQTR KYLAQSDFFW LRKAVPKTEW FTNPTTVNAF YSASTNQIRF
560 570 580 590 600
PAGELQKPFF WGTEYPRSLS YGAIGVIVGH EFTHGFDNNG RKYDKNGNLD
610 620 630 640 650
PWWSVESEEK FKEKTKCMIN QYSNYYWKKA GLNVKGKRTL GENIADNGGL
660 670 680 690 700
REAFRAYRKW INDRRQGVEE PLLPGITFTN NQLFFLSYAH VRCNSYRPEA
710 720 730 740
AREQVQIGAH SPPQFRVNGA ISNFEEFQKA FNCPRNSTMN RGADSCRLW
Length:749
Mass (Da):86,419
Last modified:February 1, 1997 - v1
Checksum:iA88FA481C376C18A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti430 – 4301D → V in AAB47750 (PubMed:9077527).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U49908 mRNA. Translation: AAC36502.1.
U73910 mRNA. Translation: AAC25962.1.
U73912 Genomic DNA. Translation: AAC25964.1.
U73913 Genomic DNA. Translation: AAC25965.1.
U73914 Genomic DNA. Translation: AAC25966.1.
U73911 Genomic DNA. Translation: AAC25963.1.
U73915 Genomic DNA. Translation: AAC25967.1.
U75646 mRNA. Translation: AAB47750.1.
CCDSiCCDS30497.1.
RefSeqiNP_035207.1. NM_011077.2.
UniGeneiMm.2529.

Genome annotation databases

EnsembliENSMUST00000079945; ENSMUSP00000078863; ENSMUSG00000057457.
GeneIDi18675.
KEGGimmu:18675.
UCSCiuc009ury.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U49908 mRNA. Translation: AAC36502.1.
U73910 mRNA. Translation: AAC25962.1.
U73912 Genomic DNA. Translation: AAC25964.1.
U73913 Genomic DNA. Translation: AAC25965.1.
U73914 Genomic DNA. Translation: AAC25966.1.
U73911 Genomic DNA. Translation: AAC25963.1.
U73915 Genomic DNA. Translation: AAC25967.1.
U75646 mRNA. Translation: AAB47750.1.
CCDSiCCDS30497.1.
RefSeqiNP_035207.1. NM_011077.2.
UniGeneiMm.2529.

3D structure databases

ProteinModelPortaliP70669.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiM13.091.

PTM databases

PhosphoSiteiP70669.

Proteomic databases

PaxDbiP70669.
PRIDEiP70669.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000079945; ENSMUSP00000078863; ENSMUSG00000057457.
GeneIDi18675.
KEGGimmu:18675.
UCSCiuc009ury.1. mouse.

Organism-specific databases

CTDi5251.
MGIiMGI:107489. Phex.

Phylogenomic databases

eggNOGiCOG3590.
HOGENOMiHOG000245574.
HOVERGENiHBG005554.
InParanoidiP70669.
KOiK08636.
OMAiEFIMNDT.
PhylomeDBiP70669.
TreeFamiTF315192.

Miscellaneous databases

NextBioi294698.
PROiP70669.
SOURCEiSearch...

Gene expression databases

BgeeiP70669.
ExpressionAtlasiP70669. baseline and differential.
GenevestigatoriP70669.

Family and domain databases

Gene3Di3.40.390.10. 2 hits.
InterProiIPR024079. MetalloPept_cat_dom.
IPR000718. Peptidase_M13.
IPR018497. Peptidase_M13_C.
IPR008753. Peptidase_M13_N.
[Graphical view]
PANTHERiPTHR11733. PTHR11733. 1 hit.
PfamiPF01431. Peptidase_M13. 1 hit.
PF05649. Peptidase_M13_N. 1 hit.
[Graphical view]
PRINTSiPR00786. NEPRILYSIN.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "cDNA cloning of the murine Pex gene implicated in X-linked hypophosphatemia and evidence for expression in bone."
    Du L., Desbarats M., Viel J., Glorieux F.H., Cawthorn C., Ecarot B.
    Genomics 36:22-28(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Pex gene deletions in Gy and Hyp mice provide mouse models for X-linked hypophosphatemia."
    Strom T.M., Francis F., Lorenz B., Boeddrich A., Econs M.J., Lehrach H., Meitinger T.
    Hum. Mol. Genet. 6:165-171(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
  3. "Pex/PEX tissue distribution and evidence for a deletion in the 3' region of the Pex gene in X-linked hypophosphatemic mice."
    Beck L., Soumounou Y., Martel J., Krishnamurthy G., Gauthier C., Goodyer C.G., Tenenhouse H.S.
    J. Clin. Invest. 99:1200-1209(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6J.

Entry informationi

Entry nameiPHEX_MOUSE
AccessioniPrimary (citable) accession number: P70669
Secondary accession number(s): P97439
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: February 4, 2015
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.