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Protein

Metalloendopeptidase homolog PEX

Gene

Phex

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probably involved in bone and dentin mineralization and renal phosphate reabsorption.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi580Zinc; catalyticPROSITE-ProRule annotation1
Active sitei581PROSITE-ProRule annotation1
Metal bindingi584Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi642Zinc; catalyticPROSITE-ProRule annotation1
Active sitei646Proton donorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • bone mineralization Source: MGI
  • organophosphate metabolic process Source: MGI
  • proteolysis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Biological processi

Biomineralization

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.4.24.B15. 3474.

Protein family/group databases

MEROPSiM13.091.

Names & Taxonomyi

Protein namesi
Recommended name:
Metalloendopeptidase homolog PEX (EC:3.4.24.-)
Alternative name(s):
Phosphate regulating neutral endopeptidase
Vitamin D-resistant hypophosphatemic rickets protein
X-linked hypophosphatemia protein
Short name:
HYP
Gene namesi
Name:Phex
Synonyms:Hyp, Pex
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:107489. Phex.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 20CytoplasmicSequence analysisAdd BLAST20
Transmembranei21 – 37Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST17
Topological domaini38 – 749ExtracellularSequence analysisAdd BLAST712

GO - Cellular componenti

  • endoplasmic reticulum Source: MGI
  • Golgi apparatus Source: MGI
  • integral component of membrane Source: UniProtKB-KW
  • perinuclear region of cytoplasm Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000782291 – 749Metalloendopeptidase homolog PEXAdd BLAST749

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi71N-linked (GlcNAc...)Sequence analysis1
Glycosylationi238N-linked (GlcNAc...)Sequence analysis1
Glycosylationi263N-linked (GlcNAc...)Sequence analysis1
Glycosylationi290N-linked (GlcNAc...)Sequence analysis1
Glycosylationi301N-linked (GlcNAc...)Sequence analysis1
Glycosylationi377N-linked (GlcNAc...)Sequence analysis1
Glycosylationi484N-linked (GlcNAc...)Sequence analysis1
Glycosylationi736N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP70669.
PRIDEiP70669.

PTM databases

iPTMnetiP70669.
PhosphoSitePlusiP70669.

Expressioni

Tissue specificityi

Bone.

Gene expression databases

BgeeiENSMUSG00000057457.
ExpressionAtlasiP70669. baseline and differential.
GenevisibleiP70669. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000078863.

Structurei

3D structure databases

ProteinModelPortaliP70669.
SMRiP70669.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M13 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3624. Eukaryota.
COG3590. LUCA.
GeneTreeiENSGT00760000119162.
HOGENOMiHOG000245574.
HOVERGENiHBG005554.
InParanoidiP70669.
KOiK08636.
OMAiANYLVWR.
OrthoDBiEOG091G025Y.
PhylomeDBiP70669.
TreeFamiTF315192.

Family and domain databases

CDDicd08662. M13. 1 hit.
Gene3Di3.40.390.10. 2 hits.
InterProiIPR024079. MetalloPept_cat_dom.
IPR000718. Peptidase_M13.
IPR018497. Peptidase_M13_C.
IPR008753. Peptidase_M13_N.
[Graphical view]
PANTHERiPTHR11733. PTHR11733. 1 hit.
PfamiPF01431. Peptidase_M13. 1 hit.
PF05649. Peptidase_M13_N. 1 hit.
[Graphical view]
PRINTSiPR00786. NEPRILYSIN.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P70669-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEAETGSTME TGKGTNRGIR IALALFIGGT LVLGTLLFLV SQGLLSFQAK
60 70 80 90 100
QEYCLKPECI EAAAAIMSKV NLSVDPCENF FRFACDGWIS NNPIPEDMPS
110 120 130 140 150
YGVYPWLRHN VDLKLKALLE KSVSRRRDTE AVQKAKILYS SCMNEKAIEK
160 170 180 190 200
ADAKPLLHIL RHSPFRWPVL EANIGPEGVW SERKFSLLQT LATFRGQYSN
210 220 230 240 250
SVFIRLYVSP DDKASNEHIL KLDQATLSLA VREDFLDNTT EAKSYRDALY
260 270 280 290 300
KFMVDTAVLL GANSSRAEHD MKSVLRLEIK IAEIMIPHEN RTSEAMYNKM
310 320 330 340 350
NISELSAMIP QFDWLGYIKK VIDTRLYPHL KDIGPSENVV VRVPQYFKDL
360 370 380 390 400
FRILGAERKK TIANYLVWRM VYSRIPNLSR RFQYRWLEFS RVIQGTTTLL
410 420 430 440 450
PQWDKCVNFI ESALPYVVGK MFVNVHFQED KKEMMEELIE GVRWAFIDML
460 470 480 490 500
EKENEWMDAG TKRKAQEKAR AVLAKVGYPE FIMNDTYVNE DLKAIKFSES
510 520 530 540 550
DYFGNVLQTR KYLAQSDFFW LRKAVPKTEW FTNPTTVNAF YSASTNQIRF
560 570 580 590 600
PAGELQKPFF WGTEYPRSLS YGAIGVIVGH EFTHGFDNNG RKYDKNGNLD
610 620 630 640 650
PWWSVESEEK FKEKTKCMIN QYSNYYWKKA GLNVKGKRTL GENIADNGGL
660 670 680 690 700
REAFRAYRKW INDRRQGVEE PLLPGITFTN NQLFFLSYAH VRCNSYRPEA
710 720 730 740
AREQVQIGAH SPPQFRVNGA ISNFEEFQKA FNCPRNSTMN RGADSCRLW
Length:749
Mass (Da):86,419
Last modified:February 1, 1997 - v1
Checksum:iA88FA481C376C18A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti430D → V in AAB47750 (PubMed:9077527).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U49908 mRNA. Translation: AAC36502.1.
U73910 mRNA. Translation: AAC25962.1.
U73912 Genomic DNA. Translation: AAC25964.1.
U73913 Genomic DNA. Translation: AAC25965.1.
U73914 Genomic DNA. Translation: AAC25966.1.
U73911 Genomic DNA. Translation: AAC25963.1.
U73915 Genomic DNA. Translation: AAC25967.1.
U75646 mRNA. Translation: AAB47750.1.
CCDSiCCDS30497.1.
RefSeqiNP_035207.1. NM_011077.2.
UniGeneiMm.2529.

Genome annotation databases

EnsembliENSMUST00000079945; ENSMUSP00000078863; ENSMUSG00000057457.
GeneIDi18675.
KEGGimmu:18675.
UCSCiuc009ury.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U49908 mRNA. Translation: AAC36502.1.
U73910 mRNA. Translation: AAC25962.1.
U73912 Genomic DNA. Translation: AAC25964.1.
U73913 Genomic DNA. Translation: AAC25965.1.
U73914 Genomic DNA. Translation: AAC25966.1.
U73911 Genomic DNA. Translation: AAC25963.1.
U73915 Genomic DNA. Translation: AAC25967.1.
U75646 mRNA. Translation: AAB47750.1.
CCDSiCCDS30497.1.
RefSeqiNP_035207.1. NM_011077.2.
UniGeneiMm.2529.

3D structure databases

ProteinModelPortaliP70669.
SMRiP70669.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000078863.

Protein family/group databases

MEROPSiM13.091.

PTM databases

iPTMnetiP70669.
PhosphoSitePlusiP70669.

Proteomic databases

PaxDbiP70669.
PRIDEiP70669.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000079945; ENSMUSP00000078863; ENSMUSG00000057457.
GeneIDi18675.
KEGGimmu:18675.
UCSCiuc009ury.1. mouse.

Organism-specific databases

CTDi5251.
MGIiMGI:107489. Phex.

Phylogenomic databases

eggNOGiKOG3624. Eukaryota.
COG3590. LUCA.
GeneTreeiENSGT00760000119162.
HOGENOMiHOG000245574.
HOVERGENiHBG005554.
InParanoidiP70669.
KOiK08636.
OMAiANYLVWR.
OrthoDBiEOG091G025Y.
PhylomeDBiP70669.
TreeFamiTF315192.

Enzyme and pathway databases

BRENDAi3.4.24.B15. 3474.

Miscellaneous databases

PROiP70669.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000057457.
ExpressionAtlasiP70669. baseline and differential.
GenevisibleiP70669. MM.

Family and domain databases

CDDicd08662. M13. 1 hit.
Gene3Di3.40.390.10. 2 hits.
InterProiIPR024079. MetalloPept_cat_dom.
IPR000718. Peptidase_M13.
IPR018497. Peptidase_M13_C.
IPR008753. Peptidase_M13_N.
[Graphical view]
PANTHERiPTHR11733. PTHR11733. 1 hit.
PfamiPF01431. Peptidase_M13. 1 hit.
PF05649. Peptidase_M13_N. 1 hit.
[Graphical view]
PRINTSiPR00786. NEPRILYSIN.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPHEX_MOUSE
AccessioniPrimary (citable) accession number: P70669
Secondary accession number(s): P97439
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: November 2, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.